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The map label for this gene is gadB [H]

Identifier: 148663297

GI number: 148663297

Start: 3859050

End: 3860432

Strand: Reverse

Name: gadB [H]

Synonym: MRA_3473

Alternate gene names: 148663297

Gene position: 3860432-3859050 (Counterclockwise)

Preceding gene: 148663298

Following gene: 148663296

Centisome position: 87.34

GC content: 65.87

Gene sequence:

>1383_bases
GTGTCTCGCAGTCACCCGTCCGTGCCCGCGCATTCGATCGCTCCGGCCTACACCGGTCGGATGTTCACCGCACCGGTGCC
GGCGCTGCGGATGCCCGATGAGTCGATGGATCCCGAGGCGGCCTATCGCTTCATCCACGACGAGCTGATGCTCGACGGTA
GCTCGCGGCTGAATCTGGCCACCTTCGTGACCACCTGGATGGACCCGGAGGCCGAAAAGCTGATGGCCGAGACGTTCGAC
AAGAACATGATCGATAAGGACGAATACCCGGCGACCGCGGCCATCGAGGCGCGCTGTGTGTCCATGGTCGCCGACCTGTT
TCACGCCGAGGGTCTGCGCGACCACGACCCCACCAGCGCCACCGGGGTGTCCACCATCGGCTCCAGCGAGGCGGTGATGC
TGGGTGGGCTGGCCCTGAAATGGCGTTGGCGGCAACGGGTGGGGTCTTGGAAGGGGCGCATGCCCAATCTGGTGATGGGT
TCGAACGTCCAGGTGGTGTGGGAGAAGTTCTGCCGCTACTTCGACGTCGAACCCCGTTACCTGCCGATGGAGCGGGGCCG
CTACGTCATCACCCCCGAGCAGGTGCTCGCCGCCGTCGACGAGAACACCATCGGCGTGGTGGCGATCTTGGGCACCACCT
ATACCGGTGAACTCGAACCCATCGCCGAGATCTGCGCCGCGCTGGACAAACTGGCGGCTGGCGGGGGTGTGGACGTCCCG
GTACACGTCGACGCGGCCAGTGGGGGCTTTGTGGTGCCGTTTTTGCATCCGGACCTGGTATGGGATTTTCGGCTGCCCCG
CGTGGTGTCGATCAACGTCAGCGGCCACAAGTATGGGCTGACCTATCCCGGCGTCGGGTTTGTCGTGTGGCGCGGGCCCG
AGCACCTGCCGGAGGATCTGGTTTTCCGGGTCAACTACCTCGGCGGCGACATGCCGACCTTCACCCTGAACTTCTCCCGT
CCCGGTAACCAGGTGGTGGGCCAGTACTACAACTTCCTGCGGCTGGGGCGCGACGGCTATACCAAGGTGATGCAGGCGCT
GTCGCACACCGCCCGGTGGCTGGGTGACCAGCTGCGCGAGGTGGATCATTGCGAGGTGATCTCGGATGGTTCGGCGATCC
CGGTGGTCAGCTTCCGGCTCGCCGGCGACCGCGGGTACACGGAGTTCGACGTCTCCCATGAGCTGCGGACCTTCGGGTGG
CAGGTGCCCGCCTACACCATGCCGGACAACGCCACCGACGTGGCGGTGCTGCGGATCGTGGTTCGGGAAGGACTCTCCGC
CGACCTGGCGCGGGCCCTGCACGACGACGCGGTCACCGCGTTGGCTGCCCTGGACAAGGTCAAGCCGGGTGGCCACTTCG
ACGCACAGCATTTCGCGCACTAG

Upstream 100 bases:

>100_bases
GATGCGCCCACGTCGGCGTCGCGCATCAGCGGCCACATTCGGGCCGCTCTGGCTGCCCTGTAGTCCTGCCAGTCCGCAAC
AAGCCCGGAAAGGATCCGCC

Downstream 100 bases:

>100_bases
TACTGCAGCCGGTGCGCCCAGTGATCAGGAGGTGATGTCTTAGGTCATGACCTGATCAAAGCTCGTCGTCGCCTGCCGGT
GGGAGTCCGGTCCGGGTAGC

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSRSHPSVPAHSIAPAYTGRMFTAPVPALRMPDESMDPEAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFD
KNMIDKDEYPATAAIEARCVSMVADLFHAEGLRDHDPTSATGVSTIGSSEAVMLGGLALKWRWRQRVGSWKGRMPNLVMG
SNVQVVWEKFCRYFDVEPRYLPMERGRYVITPEQVLAAVDENTIGVVAILGTTYTGELEPIAEICAALDKLAAGGGVDVP
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDLVFRVNYLGGDMPTFTLNFSR
PGNQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLREVDHCEVISDGSAIPVVSFRLAGDRGYTEFDVSHELRTFGW
QVPAYTMPDNATDVAVLRIVVREGLSADLARALHDDAVTALAALDKVKPGGHFDAQHFAH

Sequences:

>Translated_460_residues
MSRSHPSVPAHSIAPAYTGRMFTAPVPALRMPDESMDPEAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFD
KNMIDKDEYPATAAIEARCVSMVADLFHAEGLRDHDPTSATGVSTIGSSEAVMLGGLALKWRWRQRVGSWKGRMPNLVMG
SNVQVVWEKFCRYFDVEPRYLPMERGRYVITPEQVLAAVDENTIGVVAILGTTYTGELEPIAEICAALDKLAAGGGVDVP
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDLVFRVNYLGGDMPTFTLNFSR
PGNQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLREVDHCEVISDGSAIPVVSFRLAGDRGYTEFDVSHELRTFGW
QVPAYTMPDNATDVAVLRIVVREGLSADLARALHDDAVTALAALDKVKPGGHFDAQHFAH
>Mature_459_residues
SRSHPSVPAHSIAPAYTGRMFTAPVPALRMPDESMDPEAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAEKLMAETFDK
NMIDKDEYPATAAIEARCVSMVADLFHAEGLRDHDPTSATGVSTIGSSEAVMLGGLALKWRWRQRVGSWKGRMPNLVMGS
NVQVVWEKFCRYFDVEPRYLPMERGRYVITPEQVLAAVDENTIGVVAILGTTYTGELEPIAEICAALDKLAAGGGVDVPV
HVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDLVFRVNYLGGDMPTFTLNFSRP
GNQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLREVDHCEVISDGSAIPVVSFRLAGDRGYTEFDVSHELRTFGWQ
VPAYTMPDNATDVAVLRIVVREGLSADLARALHDDAVTALAALDKVKPGGHFDAQHFAH

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=335, Percent_Identity=24.1791044776119, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1787769, Length=440, Percent_Identity=50.2272727272727, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1789934, Length=440, Percent_Identity=50.2272727272727, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25148342, Length=351, Percent_Identity=29.3447293447293, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17543922, Length=398, Percent_Identity=24.8743718592965, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557272, Length=346, Percent_Identity=23.9884393063584, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323905, Length=466, Percent_Identity=39.9141630901288, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6320500, Length=332, Percent_Identity=25.6024096385542, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355963, Length=395, Percent_Identity=24.8101265822785, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24654344, Length=395, Percent_Identity=24.8101265822785, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 50780; Mature: 50649

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSHPSVPAHSIAPAYTGRMFTAPVPALRMPDESMDPEAAYRFIHDELMLDGSSRLNLA
CCCCCCCCCCHHCCHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEE
TFVTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIEARCVSMVADLFHAEGLRDHDPTSA
EEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TGVSTIGSSEAVMLGGLALKWRWRQRVGSWKGRMPNLVMGSNVQVVWEKFCRYFDVEPRY
CCCCCCCCCCEEEECCEEEEEHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCE
LPMERGRYVITPEQVLAAVDENTIGVVAILGTTYTGELEPIAEICAALDKLAAGGGVDVP
EHHHCCCEEECHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDL
EEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCEECCCCCCCEEEEECCCCCCCHHH
VFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLRE
EEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
VDHCEVISDGSAIPVVSFRLAGDRGYTEFDVSHELRTFGWQVPAYTMPDNATDVAVLRIV
CCCCEEECCCCCCEEEEEEEECCCCCEEEECCHHHHHHCCCCCEEECCCCCHHHHHHHHH
VREGLSADLARALHDDAVTALAALDKVKPGGHFDAQHFAH
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
SRSHPSVPAHSIAPAYTGRMFTAPVPALRMPDESMDPEAAYRFIHDELMLDGSSRLNLA
CCCCCCCCCHHCCHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEE
TFVTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIEARCVSMVADLFHAEGLRDHDPTSA
EEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TGVSTIGSSEAVMLGGLALKWRWRQRVGSWKGRMPNLVMGSNVQVVWEKFCRYFDVEPRY
CCCCCCCCCCEEEECCEEEEEHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCE
LPMERGRYVITPEQVLAAVDENTIGVVAILGTTYTGELEPIAEICAALDKLAAGGGVDVP
EHHHCCCEEECHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
VHVDAASGGFVVPFLHPDLVWDFRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDL
EEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCEECCCCCCCEEEEECCCCCCCHHH
VFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLRE
EEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
VDHCEVISDGSAIPVVSFRLAGDRGYTEFDVSHELRTFGWQVPAYTMPDNATDVAVLRIV
CCCCEEECCCCCCEEEEEEEECCCCCEEEECCHHHHHHCCCCCEEECCCCCHHHHHHHHH
VREGLSADLARALHDDAVTALAALDKVKPGGHFDAQHFAH
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]