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The map label for this gene is alkB [H]

Identifier: 148663115

GI number: 148663115

Start: 3641521

End: 3642771

Strand: Reverse

Name: alkB [H]

Synonym: MRA_3293

Alternate gene names: 148663115

Gene position: 3642771-3641521 (Counterclockwise)

Preceding gene: 148663116

Following gene: 148663114

Centisome position: 82.42

GC content: 64.11

Gene sequence:

>1251_bases
ATGACCACGCAAATCGGCTCTGGTGGACCCGAAGCCCCCCGGCCGCCCGAAGTCGAGGAGTGGCGGGACAAGAAGCGTTA
CCTGTGGCTTATGGGCCTCATCGCCCCGACGGCCTTGGTGGTGATGCTGCCGCTGATCTGGGGGATGAACCAGCTCGGCT
GGCACGCCGCCGCGCAGGTGCCGCTGTGGATCGGACCGATCCTGCTCTACGTCTTGTTGCCGCTTCTTGACCTACGCTTC
GGGCCCGACGGGCAGAACCCGCCCGACGAGGTGACCGACCGGCTGGAGAATGACAAGTACTACCGCTACTGCACCTACAT
CTACATCCCGTTCCAGTACCTCAGCGTGGTGCTGGGCGCATACCTGTTCACCGCCGCAAACCTCAGTTGGCTCGGCTTTG
ACGGCGCCTTGAGCTGGGCGGGCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCC
CACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGTCCAAGATCACCCTCGCCCAGACCTGCTACGGGCACTT
CTACATCGAGCACAACCGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCGAGACGT
TGTGGGAGTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTC
GGCGTCAGCCCCTGGAATCCCATGACGTATCTGCGCAACGACGTGCTCAACGCGTGGCTGATGTCGGTGGTGTTGTGGGG
TGGGCTGATCGCGGTCTTCGGCCCGGCGCTGATCCCGTTCGTCATCATCCAGGCAGTCTTCGGCTTCAGCCTGTTAGAGG
CCGTCAACTACCTCGAACACTACGGACTGCTACGGCAGAAGAGCGCCAACGGCCGCTACGAGCGCTGCGCCCCGGTGCAC
AGCTGGAACTCCGACCACATCGTCACCAACCTGTTCCTCTACCACCTGCAGCGACACAGCGATCACCACGCCAACCCCAC
TCGCCGCTATCAGACACTGCGGAGCATGGCGGGTGCGCCCAACCTGCCCAGTGGGTATGCATCGATGATCTCGCTGACCT
ACTTCCCGCCGTTGTGGCGCAAGGTAATGGATCACCGGGTGCTGGAGCACTACGGCGGCGATATCACTCGGGTTAACCTG
CACCCGCGCGTGCGGGAGAAGGCACTCGCCCGTTACGGAGCCTCGGCATGA

Upstream 100 bases:

>100_bases
CGCCGCGTATTTACAAATTGCGTTATATGTCTAGACATAAGACGCAAACTGCTCTATTGTCAAATCCCAACGTGGTGTGG
CTCATGAGGAGGTTTGGCAT

Downstream 100 bases:

>100_bases
TGGCCGCCTACCGGTGCCCGGTCTGCGACTACGTCTACGACGAGGCCAACGGTGATGCCAGGGAAGGCTTCCCAGCCGGC
ACAGGCTGGGATCAAATTCC

Product: transmembrane alkane 1-monooxygenase AlkB

Products: NA

Alternate protein names: Alkane hydroxylase; AHs; Terminal alkane hydroxylase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTTQIGSGGPEAPRPPEVEEWRDKKRYLWLMGLIAPTALVVMLPLIWGMNQLGWHAAAQVPLWIGPILLYVLLPLLDLRF
GPDGQNPPDEVTDRLENDKYYRYCTYIYIPFQYLSVVLGAYLFTAANLSWLGFDGALSWAGKLGVALSVGVLGGVGINTA
HEMGHKKDSLERWLSKITLAQTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLPRSVIGGLRSAVHLEAQRLRRL
GVSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGFSLLEAVNYLEHYGLLRQKSANGRYERCAPVH
SWNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGAPNLPSGYASMISLTYFPPLWRKVMDHRVLEHYGGDITRVNL
HPRVREKALARYGASA

Sequences:

>Translated_416_residues
MTTQIGSGGPEAPRPPEVEEWRDKKRYLWLMGLIAPTALVVMLPLIWGMNQLGWHAAAQVPLWIGPILLYVLLPLLDLRF
GPDGQNPPDEVTDRLENDKYYRYCTYIYIPFQYLSVVLGAYLFTAANLSWLGFDGALSWAGKLGVALSVGVLGGVGINTA
HEMGHKKDSLERWLSKITLAQTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLPRSVIGGLRSAVHLEAQRLRRL
GVSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGFSLLEAVNYLEHYGLLRQKSANGRYERCAPVH
SWNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGAPNLPSGYASMISLTYFPPLWRKVMDHRVLEHYGGDITRVNL
HPRVREKALARYGASA
>Mature_415_residues
TTQIGSGGPEAPRPPEVEEWRDKKRYLWLMGLIAPTALVVMLPLIWGMNQLGWHAAAQVPLWIGPILLYVLLPLLDLRFG
PDGQNPPDEVTDRLENDKYYRYCTYIYIPFQYLSVVLGAYLFTAANLSWLGFDGALSWAGKLGVALSVGVLGGVGINTAH
EMGHKKDSLERWLSKITLAQTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLPRSVIGGLRSAVHLEAQRLRRLG
VSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGFSLLEAVNYLEHYGLLRQKSANGRYERCAPVHS
WNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGAPNLPSGYASMISLTYFPPLWRKVMDHRVLEHYGGDITRVNLH
PRVREKALARYGASA

Specific function: Catalyzes the hydroxylation of n-alkanes in the presence of a NADH-rubredoxin reductase and rubredoxin. It preferably hydroxylases long-chain-length alkanes with at least 12 carbon atoms [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family. AlkB subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005804 [H]

Pfam domain/function: PF00487 FA_desaturase [H]

EC number: =1.14.15.3 [H]

Molecular weight: Translated: 47015; Mature: 46884

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQIGSGGPEAPRPPEVEEWRDKKRYLWLMGLIAPTALVVMLPLIWGMNQLGWHAAAQV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCC
PLWIGPILLYVLLPLLDLRFGPDGQNPPDEVTDRLENDKYYRYCTYIYIPFQYLSVVLGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHH
YLFTAANLSWLGFDGALSWAGKLGVALSVGVLGGVGINTAHEMGHKKDSLERWLSKITLA
HHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHH
QTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLPRSVIGGLRSAVHLEAQRLRRL
HHHHHEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGFSLLEAVNYLEH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLLRQKSANGRYERCAPVHSWNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGAP
HHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
NLPSGYASMISLTYFPPLWRKVMDHRVLEHYGGDITRVNLHPRVREKALARYGASA
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTQIGSGGPEAPRPPEVEEWRDKKRYLWLMGLIAPTALVVMLPLIWGMNQLGWHAAAQV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCC
PLWIGPILLYVLLPLLDLRFGPDGQNPPDEVTDRLENDKYYRYCTYIYIPFQYLSVVLGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHH
YLFTAANLSWLGFDGALSWAGKLGVALSVGVLGGVGINTAHEMGHKKDSLERWLSKITLA
HHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHH
QTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLPRSVIGGLRSAVHLEAQRLRRL
HHHHHEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGFSLLEAVNYLEH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLLRQKSANGRYERCAPVHSWNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGAP
HHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
NLPSGYASMISLTYFPPLWRKVMDHRVLEHYGGDITRVNLHPRVREKALARYGASA
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]