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The map label for this gene is pdtaS

Identifier: 148663081

GI number: 148663081

Start: 3606812

End: 3608317

Strand: Reverse

Name: pdtaS

Synonym: MRA_3259

Alternate gene names: 148663081

Gene position: 3608317-3606812 (Counterclockwise)

Preceding gene: 148663082

Following gene: 148663078

Centisome position: 81.64

GC content: 64.74

Gene sequence:

>1506_bases
ATGTCCACACTCGGTGATCTGCTCGCCGAACACACGGTGCTGCCGGGCAGCGCGGTGGACCACCTGCATGCGGTGGTCGG
GGAGTGGCAGCTCCTTGCCGACTTGTCGTTTGCCGATTACCTGATGTGGGTTCGCCGCGACGACGGTGTCCTGGTGTGCG
TTGCGCAATGCCGGCCGAACACCGGGCCGACGGTGGTGCATACCGACGCGGTAGGCACCGTCGTCGCCGCCAATAGCATG
CCGCTGGTCGCCGCGACCTTCTCCGGTGGTGTCCCGGGACGGGAAGGCGCTGTCGGCCAACAGAATTCATGTCAACACGA
CGGCCACAGTGTCGAAGTCTCCCCGGTGCGCTTTGGCGATCAGGTGGTGGCGGTGCTGACACGGCATCAACCCGAACTGG
CGGCGCGACGTAGATCCGGCCACCTGGAGACCGCCTATCGGTTGTGCGCCACAGATCTTCTCCGGATGCTGGCGGAGGGC
ACCTTTCCCGACGCAGGGGACGTGGCGATGTCGCGATCTAGCCCGCGCGCGGGTGACGGCTTCATCCGTCTCGATGTCGA
CGGTGTGGTCTCTTACGCCAGCCCCAATGCCCTATCGGCTTACCACCGAATGGGTTTGACCACCGAGTTGGAGGGCGTCA
ATCTCATTGACGCGACGCGCCCGCTGATCTCGGACCCGTTCGAGGCGCACGAGGTAGACGAGCATGTGCAGGACTTGCTG
GCCGGGGATGGAAAGGGTATGCGGATGGAGGTCGACGCCGGCGGCGCCACGGTGCTGCTGCGGACTCTGCCGCTGGTGGT
AGCTGGTCGCAATGTCGGCGCCGCGATATTGATCCGCGACGTGACCGAGGTGAAGCGGCGCGACCGAGCCCTGATATCCA
AGGACGCCACGATCCGGGAAATCCATCATCGGGTTAAGAACAACCTGCAGACGGTGGCCGCGCTGTTGCGGCTGCAGGCT
CGCCGGACGTCCAACGCCGAGGGGCGGGAAGCGCTGATCGAGTCGGTGCGCCGAGTGTCGTCGATTGCCTTGGTCCACGA
TGCGTTGTCGATGTCGGTGGACGAGCAGGTGAACCTTGACGAGGTCATCGACCGGATTCTGCCGATCATGAACGATGTGG
CATCGGTGGACAGGCCGATCCGGATAAATCGGGTTGGCGACCTCGGTGTGCTCGACTCCGACCGCGCCACGGCGCTGATC
ATGGTGATCACCGAGCTGGTGCAGAACGCGATCGAGCATGCGTTCGACCCGGCGGCGGCGGAAGGGTCCGTGACGATTCG
AGCGGAACGCTCTGCGCGTTGGCTCGATGTCGTCGTGCACGACGACGGGCTTGGTCTGCCGCAAGGTTTCAGCCTGGAGA
AGTCGGACAGCCTGGGCCTGCAGATCGTGCGGACCTTGGTCTCTGCGGAATTGGACGGCTCGTTAGGTATGCGGGACGCC
CGCGAACGTGGCACCGATGTGGTGCTACGGGTACCGGTCGGACGCCGGGGACGGTTGATGCTGTAG

Upstream 100 bases:

>100_bases
GAAGCTGCCGGAACGGTCAGCAAGGTGGCGGTATCGGTGGGCGATGTCATTCAGGCCGGCGACCTTATCGCGGTGATCAG
CTAGTCGTTGATAGTCACTC

Downstream 100 bases:

>100_bases
CGCGCTACGCAACAGTGCGGCCCCGACGTATGTCGGGGCCGCACTGTGCAGAACTGAGTCGTCAGACCCCGGTACGGGCT
TTCGTGCGGGCGTTGCGACG

Product: putative two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSTLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPNTGPTVVHTDAVGTVVAANSM
PLVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGDQVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEG
TFPDAGDVAMSRSSPRAGDGFIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLL
AGDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIREIHHRVKNNLQTVAALLRLQA
RRTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALI
MVITELVQNAIEHAFDPAAAEGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDA
RERGTDVVLRVPVGRRGRLML

Sequences:

>Translated_501_residues
MSTLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPNTGPTVVHTDAVGTVVAANSM
PLVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGDQVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEG
TFPDAGDVAMSRSSPRAGDGFIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLL
AGDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIREIHHRVKNNLQTVAALLRLQA
RRTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALI
MVITELVQNAIEHAFDPAAAEGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDA
RERGTDVVLRVPVGRRGRLML
>Mature_500_residues
STLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPNTGPTVVHTDAVGTVVAANSMP
LVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGDQVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEGT
FPDAGDVAMSRSSPRAGDGFIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLLA
GDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIREIHHRVKNNLQTVAALLRLQAR
RTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALIM
VITELVQNAIEHAFDPAAAEGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDAR
ERGTDVVLRVPVGRRGRLML

Specific function: Member of the two-component regulatory system pdtaR/pdtaS. Autophosphorylates, probably on a histidine residue, and transfers its phosphate group to pdtaR

COG id: COG3920

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDTAS_MYCTU (O05846)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842582
- PIR:   E70596
- RefSeq:   NP_217736.1
- RefSeq:   NP_337844.1
- ProteinModelPortal:   O05846
- EnsemblBacteria:   EBMYCT00000000416
- EnsemblBacteria:   EBMYCT00000069061
- GeneID:   888801
- GeneID:   922934
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3316
- KEGG:   mtu:Rv3220c
- TIGR:   MT3316
- TubercuList:   Rv3220c
- GeneTree:   EBGT00050000014828
- HOGENOM:   HBG292517
- OMA:   ITKDATI
- ProtClustDB:   CLSK872156
- InterPro:   IPR003594
- InterPro:   IPR022066
- InterPro:   IPR011495
- InterPro:   IPR005467
- Gene3D:   G3DSA:3.30.565.10
- SMART:   SM00387

Pfam domain/function: PF12282 H_kinase_N; PF02518 HATPase_c; PF07568 HisKA_2; SSF55874 ATP_bd_ATPase

EC number: =2.7.13.3

Molecular weight: Translated: 54013; Mature: 53882

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPN
CCCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHEEEEECCCCEEEEEEECCCC
TGPTVVHTDAVGTVVAANSMPLVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGD
CCCEEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCH
QVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEGTFPDAGDVAMSRSSPRAGDG
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCC
FIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLL
EEEEEHHHEEECCCCCHHHHHHHCCCCEECCCCEEECCCCHHHCCCHHHHHHHHHHHHHH
AGDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIRE
HCCCCCEEEEECCCCCEEHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHH
IHHRVKNNLQTVAALLRLQARRTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALIMVITELVQNAIEHAFDPAAA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
EGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDA
CCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
RERGTDVVLRVPVGRRGRLML
HHCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
STLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPN
CCHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCHHHHEEEEECCCCEEEEEEECCCC
TGPTVVHTDAVGTVVAANSMPLVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGD
CCCEEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCH
QVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEGTFPDAGDVAMSRSSPRAGDG
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCC
FIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLL
EEEEEHHHEEECCCCCHHHHHHHCCCCEECCCCEEECCCCHHHCCCHHHHHHHHHHHHHH
AGDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIRE
HCCCCCEEEEECCCCCEEHHHHHHHHEECCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHH
IHHRVKNNLQTVAALLRLQARRTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLD
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALIMVITELVQNAIEHAFDPAAA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
EGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDA
CCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
RERGTDVVLRVPVGRRGRLML
HHCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036