Click here to switch to the map view.

The map label for this gene is nuoI [H]

Identifier: 148663008

GI number: 148663008

Start: 3532663

End: 3533298

Strand: Direct

Name: nuoI [H]

Synonym: MRA_3186

Alternate gene names: 148663008

Gene position: 3532663-3533298 (Clockwise)

Preceding gene: 148663007

Following gene: 148663009

Centisome position: 79.92

GC content: 64.31

Gene sequence:

>636_bases
GTGGCTAACACTGATCGTCCGGCTCTCCCCCACAAGCGGGCGGTACCCCCATCTCGGGCTGACTCCGGCCCGCGTCGTCG
CCGGACTAAGTTACTGGACGCCGTAGCCGGATTCGGGGTAACGCTTGGTTCGATGTTCAAAAAGACGGTCACCGAGGAGT
ATCCGGAAAGGCCCGGTCCGGTAGCAGCGCGCTACCACGGCCGTCATCAGCTCAACCGGTATCCGGACGGCCTGGAGAAA
TGCATCGGCTGCGAGTTGTGCGCCTGGGCCTGCCCGGCCGACGCAATCTATGTCGAGGGCGCGGACAATACCGAAGAGGA
GCGGTTTTCGCCGGGCGAACGCTACGGCCGGGTGTACCAGATTAACTATTTGCGTTGCATCGGTTGCGGTTTGTGCATCG
AGGCGTGCCCGACGCGGGCGCTGACGATGACCTATGATTACGAACTGGCCGACGACAACCGCGCCGACCTGATCTACGAG
AAGGACCGGCTGCTGGCCCCGCTGCTGCCCGAGATGGCCGCGCCGCCGCATCCGCGGACGCCCGGTGCCACCGATAAGGA
CTACTACCTAGGCAATGTGACCGCCGAGGGCTTGCGGGGCGTGCGTGAGAGCCAGACCACCGGAGATTCCCGATGA

Upstream 100 bases:

>100_bases
GCTCGCGCATCGGCACGGCAACGCGGGGACGAAGGCACCAGCCCTGAACCGGCATTTCCGACACCACCGCTGCTAGCCGG
TGCAACCAAGGAGAATGCAG

Downstream 100 bases:

>100_bases
CCGCGGTGCTGGCTTCAGATGTCATCGTCCGCACCTCCACCGGGGAAGCGGTGATGTTCTGGGTGCTCAGTGCGTTGGCG
CTGCTGGGCGCGGTCGGGGT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MANTDRPALPHKRAVPPSRADSGPRRRRTKLLDAVAGFGVTLGSMFKKTVTEEYPERPGPVAARYHGRHQLNRYPDGLEK
CIGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTYDYELADDNRADLIYE
KDRLLAPLLPEMAAPPHPRTPGATDKDYYLGNVTAEGLRGVRESQTTGDSR

Sequences:

>Translated_211_residues
MANTDRPALPHKRAVPPSRADSGPRRRRTKLLDAVAGFGVTLGSMFKKTVTEEYPERPGPVAARYHGRHQLNRYPDGLEK
CIGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTYDYELADDNRADLIYE
KDRLLAPLLPEMAAPPHPRTPGATDKDYYLGNVTAEGLRGVRESQTTGDSR
>Mature_210_residues
ANTDRPALPHKRAVPPSRADSGPRRRRTKLLDAVAGFGVTLGSMFKKTVTEEYPERPGPVAARYHGRHQLNRYPDGLEKC
IGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTYDYELADDNRADLIYEK
DRLLAPLLPEMAAPPHPRTPGATDKDYYLGNVTAEGLRGVRESQTTGDSR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=142, Percent_Identity=38.7323943661972, Blast_Score=104, Evalue=5e-23,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=42.2535211267606, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789075, Length=112, Percent_Identity=33.0357142857143, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=129, Percent_Identity=41.8604651162791, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864306, Length=147, Percent_Identity=40.1360544217687, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 23467; Mature: 23336

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTDRPALPHKRAVPPSRADSGPRRRRTKLLDAVAGFGVTLGSMFKKTVTEEYPERPGP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VAARYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQ
EEHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCHHHCCCEEE
INYLRCIGCGLCIEACPTRALTMTYDYELADDNRADLIYEKDRLLAPLLPEMAAPPHPRT
ECHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCCC
PGATDKDYYLGNVTAEGLRGVRESQTTGDSR
CCCCCCCEEECCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ANTDRPALPHKRAVPPSRADSGPRRRRTKLLDAVAGFGVTLGSMFKKTVTEEYPERPGP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VAARYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQ
EEHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCHHHCCCEEE
INYLRCIGCGLCIEACPTRALTMTYDYELADDNRADLIYEKDRLLAPLLPEMAAPPHPRT
ECHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCCCCC
PGATDKDYYLGNVTAEGLRGVRESQTTGDSR
CCCCCCCEEECCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]