Click here to switch to the map view.

The map label for this gene is hisN

Identifier: 148662992

GI number: 148662992

Start: 3515549

End: 3516331

Strand: Direct

Name: hisN

Synonym: MRA_3170

Alternate gene names: 148662992

Gene position: 3515549-3516331 (Clockwise)

Preceding gene: 148662990

Following gene: 148662993

Centisome position: 79.54

GC content: 67.18

Gene sequence:

>783_bases
GTGAGCCACGACGATCTAATGCTTGCGCTGGCTCTGGCCGACCGTGCGGACGAATTGACGCGGGTCCGGTTCGGGGCGCT
CGATCTGCGCATCGACACCAAACCGGATTTGACGCCGGTGACCGACGCCGATCGGGCGGTCGAATCCGACGTGCGCCAGA
CGCTGGGCCGCGACCGGCCCGGCGACGGCGTCTTGGGCGAGGAGTTCGGCGGATCAACGACCTTCACCGGACGGCAGTGG
ATCGTAGACCCGATCGACGGCACCAAAAACTTTGTGCGCGGGGTGCCGGTGTGGGCCAGTTTGATCGCGCTGCTTGAAGA
TGGCGTCCCGTCGGTCGGTGTGGTGAGTGCGCCGGCGCTGCAACGGCGGTGGTGGGCGGCACGCGGCCGGGGCGCGTTCG
CATCCGTCGATGGTGCGCGTCCACACCGGCTGTCGGTTTCCTCTGTGGCAGAGCTGCATTCGGCGAGCTTGTCGTTTTCC
AGTCTGTCCGGGTGGGCGCGGCCGGGTCTACGTGAACGCTTCATCGGGTTGACCGATACCGTGTGGCGCGTGCGTGCTTA
CGGCGACTTTCTGTCTTACTGCCTGGTGGCCGAGGGCGCCGTCGATATTGCCGCCGAACCGCAAGTGTCGGTATGGGATC
TGGCGGCACTGGACATCGTGGTGCGTGAGGCGGGCGGGCGGCTCACCAGCCTGGACGGCGTCGCCGGCCCACACGGGGGC
AGCGCCGTTGCAACCAACGGTCTGTTGCACGACGAGGTGCTGACACGGCTCAACGCCGGGTAA

Upstream 100 bases:

>100_bases
CTGCTGGCATGAGCCGTTATTACGCCGAGCGTGAACTCAGTGCAAGAACGCACGCGAAAAATCGCACTGGGTACACGCTC
GGCGAAAGGATGGTGCACCA

Downstream 100 bases:

>100_bases
CCTGGCGCTCGAGAGCGCCATGAGCGACCCGTTCACCATCGCAACCAAACACTGGCACCGACTGCACGACAGCCGGATCC
AGTGCGATGTATGTCCACGC

Product: putative monophosphatase

Products: NA

Alternate protein names: HolPase; Histidinol-phosphate phosphatase

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSHDDLMLALALADRADELTRVRFGALDLRIDTKPDLTPVTDADRAVESDVRQTLGRDRPGDGVLGEEFGGSTTFTGRQW
IVDPIDGTKNFVRGVPVWASLIALLEDGVPSVGVVSAPALQRRWWAARGRGAFASVDGARPHRLSVSSVAELHSASLSFS
SLSGWARPGLRERFIGLTDTVWRVRAYGDFLSYCLVAEGAVDIAAEPQVSVWDLAALDIVVREAGGRLTSLDGVAGPHGG
SAVATNGLLHDEVLTRLNAG

Sequences:

>Translated_260_residues
MSHDDLMLALALADRADELTRVRFGALDLRIDTKPDLTPVTDADRAVESDVRQTLGRDRPGDGVLGEEFGGSTTFTGRQW
IVDPIDGTKNFVRGVPVWASLIALLEDGVPSVGVVSAPALQRRWWAARGRGAFASVDGARPHRLSVSSVAELHSASLSFS
SLSGWARPGLRERFIGLTDTVWRVRAYGDFLSYCLVAEGAVDIAAEPQVSVWDLAALDIVVREAGGRLTSLDGVAGPHGG
SAVATNGLLHDEVLTRLNAG
>Mature_259_residues
SHDDLMLALALADRADELTRVRFGALDLRIDTKPDLTPVTDADRAVESDVRQTLGRDRPGDGVLGEEFGGSTTFTGRQWI
VDPIDGTKNFVRGVPVWASLIALLEDGVPSVGVVSAPALQRRWWAARGRGAFASVDGARPHRLSVSSVAELHSASLSFSS
LSGWARPGLRERFIGLTDTVWRVRAYGDFLSYCLVAEGAVDIAAEPQVSVWDLAALDIVVREAGGRLTSLDGVAGPHGGS
AVATNGLLHDEVLTRLNAG

Specific function: Catalyzes the dephosphorylation of histidinol-phosphate to histidinol, the direct precursor of histidine

COG id: COG0483

COG function: function code G; Archaeal fructose-1,6-bisphosphatase and related enzymes of inositol monophosphatase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inositol monophosphatase family

Homologues:

Organism=Homo sapiens, GI7657236, Length=240, Percent_Identity=30.4166666666667, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI5031789, Length=241, Percent_Identity=28.6307053941909, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI221625487, Length=241, Percent_Identity=28.6307053941909, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788882, Length=204, Percent_Identity=30.8823529411765, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790659, Length=190, Percent_Identity=30, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193202572, Length=237, Percent_Identity=30.379746835443, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193202570, Length=240, Percent_Identity=31.25, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320493, Length=198, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21357329, Length=200, Percent_Identity=34, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24664918, Length=212, Percent_Identity=29.7169811320755, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357957, Length=239, Percent_Identity=28.4518828451883, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24664926, Length=203, Percent_Identity=26.6009852216749, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24664922, Length=237, Percent_Identity=25.7383966244726, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISN_MYCTU (P95189)

Other databases:

- EMBL:   BX842582
- PIR:   B70646
- RefSeq:   NP_217653.1
- HSSP:   P32179
- ProteinModelPortal:   P95189
- SMR:   P95189
- EnsemblBacteria:   EBMYCT00000001771
- GeneID:   888827
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv3137
- TubercuList:   Rv3137
- GeneTree:   EBGT00050000015694
- HOGENOM:   HBG730251
- OMA:   KRDDTPV
- ProtClustDB:   CLSK872142
- GO:   GO:0040007
- InterPro:   IPR011809
- InterPro:   IPR020583
- InterPro:   IPR000760
- PANTHER:   PTHR20854
- PRINTS:   PR00377
- TIGRFAMs:   TIGR02067

Pfam domain/function: PF00459 Inositol_P

EC number: =3.1.3.15

Molecular weight: Translated: 27693; Mature: 27562

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00629 IMP_1; PS00630 IMP_2

Important sites: BINDING 67-67 BINDING 185-185 BINDING 213-213

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDDLMLALALADRADELTRVRFGALDLRIDTKPDLTPVTDADRAVESDVRQTLGRDRP
CCCCHHEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GDGVLGEEFGGSTTFTGRQWIVDPIDGTKNFVRGVPVWASLIALLEDGVPSVGVVSAPAL
CCCCCCCCCCCCCEECCCEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCEECCHHH
QRRWWAARGRGAFASVDGARPHRLSVSSVAELHSASLSFSSLSGWARPGLRERFIGLTDT
HHHHHHHCCCCCEEECCCCCCCEECHHHHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHH
VWRVRAYGDFLSYCLVAEGAVDIAAEPQVSVWDLAALDIVVREAGGRLTSLDGVAGPHGG
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
SAVATNGLLHDEVLTRLNAG
CHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SHDDLMLALALADRADELTRVRFGALDLRIDTKPDLTPVTDADRAVESDVRQTLGRDRP
CCCHHEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GDGVLGEEFGGSTTFTGRQWIVDPIDGTKNFVRGVPVWASLIALLEDGVPSVGVVSAPAL
CCCCCCCCCCCCCEECCCEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCEECCHHH
QRRWWAARGRGAFASVDGARPHRLSVSSVAELHSASLSFSSLSGWARPGLRERFIGLTDT
HHHHHHHCCCCCEEECCCCCCCEECHHHHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHH
VWRVRAYGDFLSYCLVAEGAVDIAAEPQVSVWDLAALDIVVREAGGRLTSLDGVAGPHGG
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
SAVATNGLLHDEVLTRLNAG
CHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230