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The map label for this gene is ligB [H]

Identifier: 148662915

GI number: 148662915

Start: 3437740

End: 3439263

Strand: Direct

Name: ligB [H]

Synonym: MRA_3094

Alternate gene names: 148662915

Gene position: 3437740-3439263 (Clockwise)

Preceding gene: 148662912

Following gene: 148662916

Centisome position: 77.78

GC content: 68.04

Gene sequence:

>1524_bases
GTGCTCCTTCACGACGTGGCCATCACCTCAATGGACGTGGCCGCCACCTCGTCGCGGCTGACCAAGGTCGCGCGCATCGC
CGCCCTGTTGCACCGCGCCGCGCCAGACACACAGCTGGTCACGATCATCGTGTCGTGGCTCTCCGGCGAGCTGCCGCAAC
GCCATATCGGTGTCGGGTGGGCGGCATTGCGGTCCCTACCGCCGCCCGCGCCGCAACCGGCGTTGACCGTCACCGGTGTC
GACGCCACCCTCTCTAAGATCGGCACTCTACCGGGCAAAGGGTCTCAGGCGCAGCGCGCGGCACTCGTTGCGGAATTGTT
CTCCGCCGCAACCGAAGCTGAGCAAACCTTTTTGTTGCGACTGCTCGGCGGTGAACTGCGCCAGGGCGCAAAGGGCGGGA
TCATGGCCGATGCGGTCGCCCAGGCCGCCGGGCTCCCGGCCGCGACGGTCCAACGCGCCGCGATGCTAGGCGGCGACCTG
GCGGCAGCGGCGGCGGCCGGCCTGTCCGGCGCGGCGCTGGACACCTTCACCCTGCGAGTGGGCCGACCGATAGGCCCGAT
GCTGGCACAGACCGCGACCAGCGTCCATGATGCACTCGAACGTCACGGCGGCACAACCATTTTCGAGGCTAAACTAGACG
GCGCGCGAGTGCAGATCCACCGGGCAAACGACCAGGTCAGGATCTACACCCGAAGCCTGGACGACGTCACTGCCCGGCTG
CCCGAGGTGGTGGAGGCAACACTGGCACTGCCGGTCCGGGATCTAGTGGCCGACGGCGAGGCGATCGCGCTGTGCCCGGA
CAACCGGCCGCAGCGTTTCCAGGTCACCGCATCACGGTTCGGCCGATCGGTCGATGTTGCGGCTGCCCGCGCGACGCAGC
CACTTTCGGTGTTCTTCTTCGACATCCTGCATCGGGATGGTACCGACTTGCTCGAAGCGCCGACCACCGAGCGGCTGGCC
GCCCTGGACGCACTGGTGCCGGCTCGGCACCGCGTGGACCGGCTGATCACGTCCGATCCAACGGACGCGGCCAACTTCCT
GGATGCGACGCTGGCCGCCGGCCACGAGGGGGTGATGGCCAAGGCACCGGCCGCTCGTTACCTTGCGGGTCGCCGCGGAG
CGGGCTGGCTGAAGGTCAAGCCGGTGCACACACTCGACTTGGTGGTGCTCGCGGTGGAATGGGGCTCGGGACGCCGGCGC
GGCAAGCTCTCCAATATTCACCTGGGCGCACGCGATCCGGCTACCGGTGGATTCGTGATGGTGGGCAAGACCTTCAAAGG
AATGACCGACGCCATGCTGGACTGGCAGACCACCAGGTTTCACGAGATCGCGGTGGGTCCGACAGACGGCTACGTCGTCC
AACTTAGGCCCGAGCAGGTGGTCGAGGTAGCCCTCGACGGCGTGCAAAGGTCGTCGCGCTACCCGGGCGGGCTGGCATTG
CGGTTTGCCCGCGTGGTGCGCTACCGCGCCGACAAGGACCCGGCCGAGGCCGACACCATCGATGCCGTGCGCGCGCTCTA
CTGA

Upstream 100 bases:

>100_bases
GAGTATACTAATTGTCTGTCGCGGCCGCGAGACACGGCTTGCTCGGGCACGCCAGCCTTGCCCTCGCCAACGATGTCGGC
GAGACATGCCAAGCTGAACC

Downstream 100 bases:

>100_bases
TCGCACGGCGAGAGTGACTCCTGCGACGGGACACGCCGGCTGGGCGTCGCCAGATTCACGCTCGTCGACCAAGCGGGCGG
GACAAGCAGCTGCAAGGATC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGV
DATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDL
AAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLA
ALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRR
GKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
RFARVVRYRADKDPAEADTIDAVRALY

Sequences:

>Translated_507_residues
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGV
DATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDL
AAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLA
ALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRR
GKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
RFARVVRYRADKDPAEADTIDAVRALY
>Mature_507_residues
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGV
DATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDL
AAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLA
ALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRR
GKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
RFARVVRYRADKDPAEADTIDAVRALY

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=507, Percent_Identity=28.0078895463511, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI46255052, Length=494, Percent_Identity=23.6842105263158, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI23199993, Length=494, Percent_Identity=23.6842105263158, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI148539894, Length=494, Percent_Identity=23.6842105263158, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71985269, Length=496, Percent_Identity=26.4112903225806, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320038, Length=522, Percent_Identity=28.1609195402299, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24762424, Length=506, Percent_Identity=26.4822134387352, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI85815841, Length=501, Percent_Identity=25.3493013972056, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 53737; Mature: 53737

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGW
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCHH
AALRSLPPPAPQPALTVTGVDATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLR
HHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDLAAAAAAGLSGAALDTFTLRV
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEC
GRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEEEEECHHHHHHHH
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFF
HHHHHHHHHCCHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCEEHHHHHCCCCHHHHHH
DILHRDGTDLLEAPTTERLAALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMA
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
KAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHLGARDPATGGFVM
CCCHHHHHHCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
VGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
ECCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHH
RFARVVRYRADKDPAEADTIDAVRALY
HHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGW
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCHH
AALRSLPPPAPQPALTVTGVDATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLR
HHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDLAAAAAAGLSGAALDTFTLRV
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEC
GRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARL
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEEEEECHHHHHHHH
PEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFF
HHHHHHHHHCCHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCEEHHHHHCCCCHHHHHH
DILHRDGTDLLEAPTTERLAALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMA
HHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEE
KAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHLGARDPATGGFVM
CCCHHHHHHCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
VGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLAL
ECCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCHHHH
RFARVVRYRADKDPAEADTIDAVRALY
HHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]