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The map label for this gene is fadD26

Identifier: 148662776

GI number: 148662776

Start: 3255689

End: 3257440

Strand: Direct

Name: fadD26

Synonym: MRA_2956

Alternate gene names: 148662776

Gene position: 3255689-3257440 (Clockwise)

Preceding gene: 148662775

Following gene: 148662777

Centisome position: 73.66

GC content: 60.9

Gene sequence:

>1752_bases
ATGCCGGTGACCGACCGTTCAGTGCCCTCTTTGCTGCAAGAGAGGGCCGACCAGCAGCCTGACAGCACTGCATATACGTA
CATCGACTACGGATCCGACCCCAAGGGATTTGCTGACAGCTTGACTTGGTCGCAGGTCTACAGTCGTGCATGCATCATTG
CTGAAGAACTCAAGTTATGCGGGTTACCCGGAGATCGAGTGGCGGTTTTAGCGCCACAAGGACTGGAATATGTCCTTGCA
TTCCTGGGCGCACTTCAGGCTGGATTTATCGCGGTTCCGCTGTCAACTCCACAGTATGGCATTCACGATGACCGCGTTTC
TGCGGTGTTGCAGGATTCCAAGCCGGTAGCCATTCTCACGACTTCGTCCGTGGTAGGCGATGTAACGAAATACGCAGCCA
GCCACGACGGGCAGCCTGCCCCGGTCGTAGTTGAGGTTGATCTGCTTGATTTGGACTCGCCGCGACAGATGCCGGCTTTC
TCTCGTCAGCACACCGGGGCGGCTTATCTCCAATACACGTCCGGATCGACGCGTACGCCGGCCGGAGTCATTGTGTCGCA
CACGAATGTCATTGCCAATGTGACACAAAGTATGTACGGCTATTTCGGCGATCCCGCAAAGATTCCGACCGGGACTGTGG
TGTCGTGGCTGCCTTTGTATCACGATATGGGCCTGATTCTCGGAATTTGCGCACCGCTGGTGGCCCGACGCCGCGCGATG
TTGATGAGCCCAATGTCATTTTTGCGCCGTCCGGCCCGCTGGATGCAACTGCTTGCCACCAGCGGCCGGTGCTTTTCTGC
GGCACCGAATTTCGCCTTCGAGCTGGCCGTGCGCAGAACATCTGACCAGGACATGGCGGGGCTCGACCTGCGCGACGTGG
TCGGCATCGTCAGTGGCAGTGAGCGAATCCATGTGGCAACCGTGCGGCGGTTCATCGAGCGGTTCGCGCCGTACAATCTC
AGCCCCACCGCGATACGGCCGTCGTACGGGCTCGCGGAAGCGACCTTATATGTGGCAGCTCCCGAAGCCGGCGCCGCGCC
CAAGACGGTCCGTTTTGACTACGAGCAGCTGACCGCCGGGCAGGCTCGGCCCTGCGGAACCGATGGGTCGGTCGGCACCG
AACTGATCAGCTACGGCTCCCCCGACCCATCGTCTGTGCGAATCGTCAACCCGGAGACCATGGTTGAGAATCCGCCTGGA
GTGGTCGGTGAGATCTGGGTGCATGGCGACCACGTGACTATGGGGTATTGGCAGAAGCCGAAGCAGACCGCGCAGGTCTT
CGACGCCAAGCTGGTCGATCCCGCGCCGGCAGCCCCGGAGGGGCCGTGGCTGCGCACCGGCGACCTGGGCGTCATTTCCG
ATGGTGAGCTGTTCATCATGGGCCGCATCAAAGACCTGCTCATCGTGGACGGGCGCAACCACTACCCCGACGACATCGAG
GCAACGATCCAGGAGATCACCGGTGGACGGGCCGCGGCGATCGCAGTGCCCGACGACATCACCGAACAACTGGTGGCGAT
CATCGAATTCAAGCGACGCGGTAGTACCGCCGAAGAGGTCATGCTCAAGCTCCGCTCGGTGAAGCGTGAGGTCACCTCCG
CGATATCGAAGTCACACAGCCTGCGGGTGGCCGATCTCGTTCTGGTGTCACCTGGTTCGATTCCCATCACCACCAGCGGC
AAGATCCGGCGGTCAGCCTGCGTCGAACGCTATCGCAGCGACGGCTTCAAGCGGCTGGACGTAGCCGTATGA

Upstream 100 bases:

>100_bases
TCAGAGCGGTGGGGATGTCCCGACGTGCGATCCGACAGGGGTTCGCAGGGTCCGCAAAAAACATAGTGAACGCCAGAAAG
CCGAATGGGAGTACAAGGCG

Downstream 100 bases:

>100_bases
CGGGAAGCATCAGTGGTGAAGCCGACCTTCGCCACTGGCTAATCGACTACCTAGTAACCAATATCGGCTGCACACCTGAC
GAGGTGGACCCCGATCTGTC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLA
FLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAF
SRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAM
LMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNL
SPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPG
VVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIE
ATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSG
KIRRSACVERYRSDGFKRLDVAV

Sequences:

>Translated_583_residues
MPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLA
FLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAF
SRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAM
LMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNL
SPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPG
VVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIE
ATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSG
KIRRSACVERYRSDGFKRLDVAV
>Mature_582_residues
PVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAF
LGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFS
RQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAML
MSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLS
PTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGV
VGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEA
TIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGK
IRRSACVERYRSDGFKRLDVAV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI44888818, Length=613, Percent_Identity=26.2642740619902, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI55749758, Length=605, Percent_Identity=26.1157024793388, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI225735629, Length=604, Percent_Identity=24.1721854304636, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI45827692, Length=604, Percent_Identity=24.1721854304636, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI225735625, Length=609, Percent_Identity=25.7799671592775, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI187761345, Length=573, Percent_Identity=24.956369982548, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI187761343, Length=573, Percent_Identity=24.956369982548, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1788107, Length=586, Percent_Identity=25.7679180887372, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=598, Percent_Identity=22.5752508361204, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI193202679, Length=620, Percent_Identity=24.3548387096774, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=498, Percent_Identity=26.7068273092369, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654656, Length=475, Percent_Identity=23.5789473684211, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281365686, Length=171, Percent_Identity=30.9941520467836, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FAD26_MYCTU (Q10976)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   B70749
- RefSeq:   NP_217446.2
- RefSeq:   NP_337513.1
- ProteinModelPortal:   Q10976
- EnsemblBacteria:   EBMYCT00000002263
- EnsemblBacteria:   EBMYCT00000069971
- GeneID:   887603
- GeneID:   925276
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2999
- KEGG:   mtu:Rv2930
- TIGR:   MT2999
- TubercuList:   Rv2930
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   PARWIQQ
- ProtClustDB:   PRK05850
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63045; Mature: 62913

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLC
CCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHC
GLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILT
CCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEE
TSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTP
CCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
AGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAM
CCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGS
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCC
ERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAG
CEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHCCC
QARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKP
CCCCCCCCCCHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCEEEEEEECCEEEECCCCCC
KQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIE
HHHHHHHHHHCCCCCCCCCCCCEEECCCEEEECCCCEEEEECCCEEEEECCCCCCCCHHH
ATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHS
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRLDVAV
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
PVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLC
CCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHC
GLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILT
CCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEE
TSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTP
CCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
AGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAM
CCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGS
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCC
ERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAG
CEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHCCC
QARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKP
CCCCCCCCCCHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCEEEEEEECCEEEECCCCCC
KQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIE
HHHHHHHHHHCCCCCCCCCCCCEEECCCEEEECCCCEEEEECCCEEEEECCCCCCCCHHH
ATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHS
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRLDVAV
EEEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036