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The map label for this gene is hemN

Identifier: 148662221

GI number: 148662221

Start: 2693983

End: 2695110

Strand: Reverse

Name: hemN

Synonym: MRA_2412

Alternate gene names: 148662221

Gene position: 2695110-2693983 (Counterclockwise)

Preceding gene: 148662222

Following gene: 148662218

Centisome position: 60.98

GC content: 69.06

Gene sequence:

>1128_bases
ATGCCCGGACAACCGTTCGGGGTCTACCTGCACGTCCCGTTCTGCCTGACCCGTTGCGGGTACTGCGACTTCAATACCTA
CACCCCGGCCCAGCTGGGTGGCGTCAGCCCGGACCGTTGGCTGCTGGCGCTGCGGGCGGAGCTCGAACTGGCGGCCGCCA
AGCTGGACGCACCGACGGTGCATACCGTGTATGTGGGTGGCGGGACGCCATCGCTGCTCGGGGGGGAGCGCCTGGCCACG
TTGCTGGACATGGTGCGGGACCACTTTGTGCTGGCGCCCGACGCCGAAGTCAGCACCGAGGCCAACCCCGAGTCGACGTG
GCCGGAGTTCTTCGCCACGATCCGCGCGGCCGGTTACACGCGGGTGTCGCTCGGCATGCAGTCGGTGGCCCCGAGGGTGC
TGGCAACCTTGGACCGGGTGCACTCGCCGGGCCGGGCGGCGGCCGCGGCCACCGAGGCGATAGCGGAGGGCTTCACACAC
GTCAACCTCGACCTGATCTATGGAACCCCGGGGGAGTCCGACGACGACCTGGTGCGCTCAGTGGACGCCGCGGTGCAGGC
CGGTGTGGATCATGTGTCCGCGTATGCCTTGGTCGTCGAGCACGGCACGGCACTGGCTCGCCGGGTTCGGCGTGGTGAGC
TGGCCGCGCCCGATGACGACGTGCTGGCGCATCGCTACGAGTTGGTCGATGCTCGGCTGTCGGCGGCCGGGTTTGCCTGG
TACGAGGTGTCCAATTGGTGTCGACCGGGCGGCGAGTGCCGGCACAACCTGGGTTATTGGGACGGTGGCCAGTGGTGGGG
CGCAGGTCCGGGCGCGCACGGCTACATTGGCGTGACACGCTGGTGGAATGTCAAGCACCCCAACACTTACGCCGAGATAC
TGGCCGGGGCGACGTTGCCGGTAGCGGGCTTCGAGCAGCTGGGCGCCGACGCTTTGCACACCGAAGACGTGTTGCTGAAG
GTCCGGCTGCGCCAGGGGCTACCGCTCGCCCGGCTAGGGGCTGCCGAACGGGAACGTGCCGAGGCGGTGTTGGCCGACGG
ATTGCTGGACTACCACGGCGACCGACTGGTCCTCACCGGCCGGGGGCGATTGCTGGCCGACGCGGTGGTGCGCACTCTGC
TGGGCTAA

Upstream 100 bases:

>100_bases
GCCTGCGTTACATTGCTGGCCACCCTAGCTGGTACGCGCTATGCGATGATTTGGCATGGTATTTCGGCAGGCACCAGTTG
AGCTGCCCGGCCTGGCGCCC

Downstream 100 bases:

>100_bases
CCGATCGAAGCCCCGGTGGCAGGGAACCATTGCCCGACGATGCGGGCGATCTCGATGTACAGCGGGATCCCGATCGTGAC
GTTGTAGGAGAACGTCAAAC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MPGQPFGVYLHVPFCLTRCGYCDFNTYTPAQLGGVSPDRWLLALRAELELAAAKLDAPTVHTVYVGGGTPSLLGGERLAT
LLDMVRDHFVLAPDAEVSTEANPESTWPEFFATIRAAGYTRVSLGMQSVAPRVLATLDRVHSPGRAAAAATEAIAEGFTH
VNLDLIYGTPGESDDDLVRSVDAAVQAGVDHVSAYALVVEHGTALARRVRRGELAAPDDDVLAHRYELVDARLSAAGFAW
YEVSNWCRPGGECRHNLGYWDGGQWWGAGPGAHGYIGVTRWWNVKHPNTYAEILAGATLPVAGFEQLGADALHTEDVLLK
VRLRQGLPLARLGAAERERAEAVLADGLLDYHGDRLVLTGRGRLLADAVVRTLLG

Sequences:

>Translated_375_residues
MPGQPFGVYLHVPFCLTRCGYCDFNTYTPAQLGGVSPDRWLLALRAELELAAAKLDAPTVHTVYVGGGTPSLLGGERLAT
LLDMVRDHFVLAPDAEVSTEANPESTWPEFFATIRAAGYTRVSLGMQSVAPRVLATLDRVHSPGRAAAAATEAIAEGFTH
VNLDLIYGTPGESDDDLVRSVDAAVQAGVDHVSAYALVVEHGTALARRVRRGELAAPDDDVLAHRYELVDARLSAAGFAW
YEVSNWCRPGGECRHNLGYWDGGQWWGAGPGAHGYIGVTRWWNVKHPNTYAEILAGATLPVAGFEQLGADALHTEDVLLK
VRLRQGLPLARLGAAERERAEAVLADGLLDYHGDRLVLTGRGRLLADAVVRTLLG
>Mature_374_residues
PGQPFGVYLHVPFCLTRCGYCDFNTYTPAQLGGVSPDRWLLALRAELELAAAKLDAPTVHTVYVGGGTPSLLGGERLATL
LDMVRDHFVLAPDAEVSTEANPESTWPEFFATIRAAGYTRVSLGMQSVAPRVLATLDRVHSPGRAAAAATEAIAEGFTHV
NLDLIYGTPGESDDDLVRSVDAAVQAGVDHVSAYALVVEHGTALARRVRRGELAAPDDDVLAHRYELVDARLSAAGFAWY
EVSNWCRPGGECRHNLGYWDGGQWWGAGPGAHGYIGVTRWWNVKHPNTYAEILAGATLPVAGFEQLGADALHTEDVLLKV
RLRQGLPLARLGAAERERAEAVLADGLLDYHGDRLVLTGRGRLLADAVVRTLLG

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI8922911, Length=273, Percent_Identity=38.0952380952381, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1789325, Length=382, Percent_Identity=33.2460732984293, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI87082341, Length=306, Percent_Identity=26.1437908496732, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMN_MYCTU (P71756)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   G70681
- RefSeq:   NP_216904.1
- RefSeq:   NP_336938.1
- ProteinModelPortal:   P71756
- SMR:   P71756
- EnsemblBacteria:   EBMYCT00000001339
- EnsemblBacteria:   EBMYCT00000072179
- GeneID:   885300
- GeneID:   925891
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2457
- KEGG:   mtu:Rv2388c
- TIGR:   MT2457
- TubercuList:   Rv2388c
- GeneTree:   EBGT00050000017191
- HOGENOM:   HBG529648
- OMA:   RTHSACD
- ProtClustDB:   PRK05628
- BRENDA:   1.3.99.22
- GO:   GO:0005737
- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197
- SMART:   SM00729
- TIGRFAMs:   TIGR00539

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM

EC number: =1.3.99.22

Molecular weight: Translated: 40334; Mature: 40203

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: BINDING 9-9 BINDING 21-21 BINDING 67-67 BINDING 100-100 BINDING 127-127 BINDING 139-139 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQPFGVYLHVPFCLTRCGYCDFNTYTPAQLGGVSPDRWLLALRAELELAAAKLDAPTV
CCCCCEEEEEECHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCE
HTVYVGGGTPSLLGGERLATLLDMVRDHFVLAPDAEVSTEANPESTWPEFFATIRAAGYT
EEEEECCCCCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCE
RVSLGMQSVAPRVLATLDRVHSPGRAAAAATEAIAEGFTHVNLDLIYGTPGESDDDLVRS
EEHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHH
VDAAVQAGVDHVSAYALVVEHGTALARRVRRGELAAPDDDVLAHRYELVDARLSAAGFAW
HHHHHHHCHHHHHHHHHEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
YEVSNWCRPGGECRHNLGYWDGGQWWGAGPGAHGYIGVTRWWNVKHPNTYAEILAGATLP
EEHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCC
VAGFEQLGADALHTEDVLLKVRLRQGLPLARLGAAERERAEAVLADGLLDYHGDRLVLTG
CCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEC
RGRLLADAVVRTLLG
CCCHHHHHHHHHHCC
>Mature Secondary Structure 
PGQPFGVYLHVPFCLTRCGYCDFNTYTPAQLGGVSPDRWLLALRAELELAAAKLDAPTV
CCCCEEEEEECHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCE
HTVYVGGGTPSLLGGERLATLLDMVRDHFVLAPDAEVSTEANPESTWPEFFATIRAAGYT
EEEEECCCCCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCE
RVSLGMQSVAPRVLATLDRVHSPGRAAAAATEAIAEGFTHVNLDLIYGTPGESDDDLVRS
EEHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHH
VDAAVQAGVDHVSAYALVVEHGTALARRVRRGELAAPDDDVLAHRYELVDARLSAAGFAW
HHHHHHHCHHHHHHHHHEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
YEVSNWCRPGGECRHNLGYWDGGQWWGAGPGAHGYIGVTRWWNVKHPNTYAEILAGATLP
EEHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCC
VAGFEQLGADALHTEDVLLKVRLRQGLPLARLGAAERERAEAVLADGLLDYHGDRLVLTG
CCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEC
RGRLLADAVVRTLLG
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036