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The map label for this gene is narK1 [H]

Identifier: 148662156

GI number: 148662156

Start: 2611818

End: 2613365

Strand: Reverse

Name: narK1 [H]

Synonym: MRA_2349

Alternate gene names: 148662156

Gene position: 2613365-2611818 (Counterclockwise)

Preceding gene: 148662157

Following gene: 148662153

Centisome position: 59.13

GC content: 61.76

Gene sequence:

>1548_bases
ATGGAACAGCACACGCTGCTGCAACGCGAGGAGTCGCCGCGCAGTCCCGCGGCCCCTTCGCTCAGGCGCCTGGGTGGCTC
ACGTCACATCACGCATTGGGATCCCGAGGATCTAGGAGCGTGGGAGGCAGGCAACAAGGGCATTGCCCGACGCAATCTGC
TTTGGTCGGTCGTGACGGTTCACCTGGGTTACTCGGTGTGGACGCTCTGGCCGGTGCTGGAGTTGCTGATGCCCCAGGAC
GTCTACGGCTTTTCAACCAGTGACAAGTTTCTGCTCGGCACCATCGCGACGCTGTTTGGCGCGTTCCTGCGGATGCCCTA
CGCGTTGGCCAGCGCGATCTTTGGCGGTCGCAACTGGGCGACTTTTTCGGCAATCGTGTTGCTAATTCCGGCCATTGGCA
CAACGGTGTTGTTGACCCATCCGGGGCTACCGCTGTGGCCGTATCTGGTGTGTGCGGCCCTGACCGGCTTAGGCGGCGGC
AACTTCGCGTCGTCAATGAGCAACGCCAATGCTTTCTATCCACATCGGCTCAAGGGTTCGGCGCTCGGAATTGCCGGTGG
GGTAGGCAATCTCGGGGTGCCGGCGATCCAGCTAGTTGGGTTGTTGGCAATCGCCACTGTCGGTGAGCGGAAGCCTTACC
TGGTCTGCGCACTGTATGTGGTTCTGGTGGCAATCGCGGTAATCGGAGTGTCGCTGTTCATGAACAATGTCGAACAGCAC
CGGGTGCAGGTGAACCGGCTTCGGCCGATCGTTTCCGCGGTTCTGTCGACCCGCGACACGTGGTTGCTCTCGCTGCTCTA
CCTCGGCACTTTCGGCTCATTCATCGGCTTCTCCTTCGTGTTTGGCCAGGTGTTGCAGACCAACTTCCTGGCGTGCGGAC
AAAGCCCGGCGCGCGCGACGCTGCATGCCGTCGAGTTGGCGTTTGTCGGGCCGTTGCTGGCGGCGGTGGCCCGGATTTAC
GGTGGCCGGCTGGCCGATCGAGTCGGTGGAAGCCGCTTGACCCTTATAGTCTTTGTGGCGATGACGCTCGCCGCTGGGCT
GCTGATCAGTGCCAGCACCCTCGAAGGCCGACATGTCGGCCAGCATCGGGGCGCTACCATGGTCGGCTACTTCGTCTGCT
TCGTCGCGTTGTTCGTCCTATCCGGGTTGGGCAACGGGTCTGTGTACAAGATGATTCCGACGATTTTTGAGGCGTGCAGC
CGCTCCCTGGATCTCAGTGAAGCCGAACGCCGCGACTGGTCACGCATCATCTCAGGAGTAGTCATCGGGTTTGTGGCCGC
CTTCGGCGCGCTCGGCGGGGTCGGAATCAATATGGCGCTGCGTGAGTCCTACCTCAGCACCGGCAGTGGGACCGATGCGT
TCTGGATCTTCATGATGTGCTACGCCGCTGCCGCCGTTCTGACTTGGAAAGTGTACGACCGCCGAACGGTCACCGACATG
GGGATGTTGCAGGCGGCCTTGGTGCGGCAGCCGGCATCGACGCCAGCCGAACTTATCGGTCCCAGAACTCAATCGGACCG
ATTCTCCGGGTGCAGCATCTCGGCGTAA

Upstream 100 bases:

>100_bases
TACAGGAGTTTCAGGCTGCCAGGAAACAACTTCGTACACGCAAGGCTGGCACCCTGGTAGGCCGGCCCGCATTCGCACCA
GGGTGGGAACAAGGAGGGTG

Downstream 100 bases:

>100_bases
CGTAGCGGCTCCGGGATGAAGCTCGACCGCCCGGCTAGGAGATCAGCACCGTGACAGCGGCATTGCCAGCCGTCGTCAGG
TGGCCAATGCCTTGAGCAAA

Product: nitrite extrusion protein 1 NarK1

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MEQHTLLQREESPRSPAAPSLRRLGGSRHITHWDPEDLGAWEAGNKGIARRNLLWSVVTVHLGYSVWTLWPVLELLMPQD
VYGFSTSDKFLLGTIATLFGAFLRMPYALASAIFGGRNWATFSAIVLLIPAIGTTVLLTHPGLPLWPYLVCAALTGLGGG
NFASSMSNANAFYPHRLKGSALGIAGGVGNLGVPAIQLVGLLAIATVGERKPYLVCALYVVLVAIAVIGVSLFMNNVEQH
RVQVNRLRPIVSAVLSTRDTWLLSLLYLGTFGSFIGFSFVFGQVLQTNFLACGQSPARATLHAVELAFVGPLLAAVARIY
GGRLADRVGGSRLTLIVFVAMTLAAGLLISASTLEGRHVGQHRGATMVGYFVCFVALFVLSGLGNGSVYKMIPTIFEACS
RSLDLSEAERRDWSRIISGVVIGFVAAFGALGGVGINMALRESYLSTGSGTDAFWIFMMCYAAAAVLTWKVYDRRTVTDM
GMLQAALVRQPASTPAELIGPRTQSDRFSGCSISA

Sequences:

>Translated_515_residues
MEQHTLLQREESPRSPAAPSLRRLGGSRHITHWDPEDLGAWEAGNKGIARRNLLWSVVTVHLGYSVWTLWPVLELLMPQD
VYGFSTSDKFLLGTIATLFGAFLRMPYALASAIFGGRNWATFSAIVLLIPAIGTTVLLTHPGLPLWPYLVCAALTGLGGG
NFASSMSNANAFYPHRLKGSALGIAGGVGNLGVPAIQLVGLLAIATVGERKPYLVCALYVVLVAIAVIGVSLFMNNVEQH
RVQVNRLRPIVSAVLSTRDTWLLSLLYLGTFGSFIGFSFVFGQVLQTNFLACGQSPARATLHAVELAFVGPLLAAVARIY
GGRLADRVGGSRLTLIVFVAMTLAAGLLISASTLEGRHVGQHRGATMVGYFVCFVALFVLSGLGNGSVYKMIPTIFEACS
RSLDLSEAERRDWSRIISGVVIGFVAAFGALGGVGINMALRESYLSTGSGTDAFWIFMMCYAAAAVLTWKVYDRRTVTDM
GMLQAALVRQPASTPAELIGPRTQSDRFSGCSISA
>Mature_515_residues
MEQHTLLQREESPRSPAAPSLRRLGGSRHITHWDPEDLGAWEAGNKGIARRNLLWSVVTVHLGYSVWTLWPVLELLMPQD
VYGFSTSDKFLLGTIATLFGAFLRMPYALASAIFGGRNWATFSAIVLLIPAIGTTVLLTHPGLPLWPYLVCAALTGLGGG
NFASSMSNANAFYPHRLKGSALGIAGGVGNLGVPAIQLVGLLAIATVGERKPYLVCALYVVLVAIAVIGVSLFMNNVEQH
RVQVNRLRPIVSAVLSTRDTWLLSLLYLGTFGSFIGFSFVFGQVLQTNFLACGQSPARATLHAVELAFVGPLLAAVARIY
GGRLADRVGGSRLTLIVFVAMTLAAGLLISASTLEGRHVGQHRGATMVGYFVCFVALFVLSGLGNGSVYKMIPTIFEACS
RSLDLSEAERRDWSRIISGVVIGFVAAFGALGGVGINMALRESYLSTGSGTDAFWIFMMCYAAAAVLTWKVYDRRTVTDM
GMLQAALVRQPASTPAELIGPRTQSDRFSGCSISA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=468, Percent_Identity=35.042735042735, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1787743, Length=480, Percent_Identity=35.4166666666667, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55137; Mature: 55137

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQHTLLQREESPRSPAAPSLRRLGGSRHITHWDPEDLGAWEAGNKGIARRNLLWSVVTV
CCCCHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHH
HLGYSVWTLWPVLELLMPQDVYGFSTSDKFLLGTIATLFGAFLRMPYALASAIFGGRNWA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TFSAIVLLIPAIGTTVLLTHPGLPLWPYLVCAALTGLGGGNFASSMSNANAFYPHRLKGS
HHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
ALGIAGGVGNLGVPAIQLVGLLAIATVGERKPYLVCALYVVLVAIAVIGVSLFMNNVEQH
CCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVQVNRLRPIVSAVLSTRDTWLLSLLYLGTFGSFIGFSFVFGQVLQTNFLACGQSPARAT
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LHAVELAFVGPLLAAVARIYGGRLADRVGGSRLTLIVFVAMTLAAGLLISASTLEGRHVG
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QHRGATMVGYFVCFVALFVLSGLGNGSVYKMIPTIFEACSRSLDLSEAERRDWSRIISGV
HHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VIGFVAAFGALGGVGINMALRESYLSTGSGTDAFWIFMMCYAAAAVLTWKVYDRRTVTDM
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GMLQAALVRQPASTPAELIGPRTQSDRFSGCSISA
HHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEQHTLLQREESPRSPAAPSLRRLGGSRHITHWDPEDLGAWEAGNKGIARRNLLWSVVTV
CCCCHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHH
HLGYSVWTLWPVLELLMPQDVYGFSTSDKFLLGTIATLFGAFLRMPYALASAIFGGRNWA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TFSAIVLLIPAIGTTVLLTHPGLPLWPYLVCAALTGLGGGNFASSMSNANAFYPHRLKGS
HHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
ALGIAGGVGNLGVPAIQLVGLLAIATVGERKPYLVCALYVVLVAIAVIGVSLFMNNVEQH
CCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVQVNRLRPIVSAVLSTRDTWLLSLLYLGTFGSFIGFSFVFGQVLQTNFLACGQSPARAT
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LHAVELAFVGPLLAAVARIYGGRLADRVGGSRLTLIVFVAMTLAAGLLISASTLEGRHVG
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QHRGATMVGYFVCFVALFVLSGLGNGSVYKMIPTIFEACSRSLDLSEAERRDWSRIISGV
HHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VIGFVAAFGALGGVGINMALRESYLSTGSGTDAFWIFMMCYAAAAVLTWKVYDRRTVTDM
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
GMLQAALVRQPASTPAELIGPRTQSDRFSGCSISA
HHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]