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The map label for this gene is sseB

Identifier: 148662113

GI number: 148662113

Start: 2573078

End: 2573932

Strand: Direct

Name: sseB

Synonym: MRA_2308

Alternate gene names: 148662113

Gene position: 2573078-2573932 (Clockwise)

Preceding gene: 148662112

Following gene: 148662116

Centisome position: 58.21

GC content: 65.85

Gene sequence:

>855_bases
GTGCAAGCCCGTGGTCAGGTCCTGATCACCGCCGCGGAACTGGCTGGCATGATCCAGGCCGGCGATCCGGTGTCGATCCT
GGATGTGCGCTGGCGGCTTGATGAACCTGACGGGCATGCGGCCTACCTACAGGGTCACCTGCCGGGAGCGGTATTTGTGT
CACTCGAGGACGAACTGAGCGATCATACGATCGCCGGCCGGGGCCGGCACCCGCTGCCGTCGGGGGCTAGTCTGCAAGCC
ACCGTCCGCCGATGCGGAATCCGACACGATGTGCCGGTCGTGGTCTACGACGACTGGAATCGAGCCGGTTCCGCGCGAGC
GTGGTGGGTGTTAACTGCGGCTGGGATCGCGAATGTACGCATTCTAGACGGCGGCTTGCCCGCGTGGCGGTCCGCAGGCG
GCAGCATCGAGACCGGCCAGGTCAGCCCGCAGCTCGGGAATGTGACTGTGCTGCACGATGATTTGTATGCCGGACAGCGG
CTAACCCTAACGGCGCAGCAAGCCGGTGCGGGTGGTGTGACGCTGCTCGATGCGCGCGTACCGGAACGTTTCCGCGGCGA
TGTCGAGCCCGTGGATGCGGTTGCCGGTCACATCCCCGGCGCCATCAACGTTCCCAGCGGTAGTGTCCTGGCCGACGACG
GCACGTTCCTTGGCAATGGCGCCCTTAACGCACTGCTGTCCGACCACGGCATCGATCACGGTGGCCGCGTGGGTGTCTAC
TGCGGCTCGGGTGTCAGCGCAGCTGTCATCGTCGCGGCACTGGCAGTGATCGGCCAGGATGCGGAGCTGTTTCCAGGGTC
ATGGTCGGAGTGGAGTTCGGATCCGACCCGTCCCGTCGGCCGTGGCACTGCATAG

Upstream 100 bases:

>100_bases
TGCGTGAGCTGCCGTTCGGGGTACATGTAACCTGTCCGTAACTACACTGATTGCGCGACAAGGGAATTAGCCGCGTTGGC
AGGCAACACGGAGGTGACCG

Downstream 100 bases:

>100_bases
TCAGACGCCGGCCCAGTTCTGCAGGAAGGCTTCGGTGACCCGGGCGGCGTTGTTGGCCGCAATCTGCTTGTAAACGAAGA
ACTGGACGGGGAAGCCCGGC

Product: putative thiosulfate sulfurtransferase SseB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELSDHTIAGRGRHPLPSGASLQA
TVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVRILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQR
LTLTAQQAGAGGVTLLDARVPERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVY
CGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA

Sequences:

>Translated_284_residues
MQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELSDHTIAGRGRHPLPSGASLQA
TVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVRILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQR
LTLTAQQAGAGGVTLLDARVPERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVY
CGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA
>Mature_284_residues
MQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELSDHTIAGRGRHPLPSGASLQA
TVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVRILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQR
LTLTAQQAGAGGVTLLDARVPERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVY
CGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains

Homologues:

Organism=Homo sapiens, GI194473668, Length=267, Percent_Identity=28.8389513108614, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI61835204, Length=267, Percent_Identity=28.8389513108614, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI194473681, Length=267, Percent_Identity=28.8389513108614, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI17402865, Length=282, Percent_Identity=29.7872340425532, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87082121, Length=241, Percent_Identity=35.6846473029046, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI87081967, Length=255, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71997283, Length=252, Percent_Identity=32.5396825396825, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17561888, Length=243, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115534702, Length=287, Percent_Identity=24.0418118466899, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): THT3_MYCTU (Q59570)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   G70732
- RefSeq:   NP_216807.1
- RefSeq:   NP_336819.1
- ProteinModelPortal:   Q59570
- SMR:   Q59570
- EnsemblBacteria:   EBMYCT00000001622
- EnsemblBacteria:   EBMYCT00000070448
- GeneID:   887174
- GeneID:   924074
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2348
- KEGG:   mtu:Rv2291
- TIGR:   MT2348
- TubercuList:   Rv2291
- GeneTree:   EBGT00050000015573
- HOGENOM:   HBG709927
- OMA:   LARITTD
- ProtClustDB:   CLSK791756
- BRENDA:   2.8.1.1
- InterPro:   IPR001763
- InterPro:   IPR001307
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 29400; Mature: 29400

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3

Important sites: ACT_SITE 241-241 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELS
CCCCCCEEEEHHHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEEEECCCCC
DHTIAGRGRHPLPSGASLQATVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVR
CCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCEEE
ILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQRLTLTAQQAGAGGVTLLDARV
EEECCCCHHHCCCCCEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEECCC
PERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVY
CHHHCCCCCHHHHHHHCCCCEEECCCCCEEECCCCEECCHHHHHHHHHCCCCCCCEEEEE
CGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA
ECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELS
CCCCCCEEEEHHHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEEECCCCEEEEEECCCCC
DHTIAGRGRHPLPSGASLQATVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVR
CCEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCEEE
ILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQRLTLTAQQAGAGGVTLLDARV
EEECCCCHHHCCCCCEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEECCC
PERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVY
CHHHCCCCCHHHHHHHCCCCEEECCCCCEEECCCCEECCHHHHHHHHHCCCCCCCEEEEE
CGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA
ECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036