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The map label for this gene is glnA3 [H]

Identifier: 148661684

GI number: 148661684

Start: 2137954

End: 2139306

Strand: Direct

Name: glnA3 [H]

Synonym: MRA_1889

Alternate gene names: 148661684

Gene position: 2137954-2139306 (Clockwise)

Preceding gene: 148661683

Following gene: 148661685

Centisome position: 48.37

GC content: 64.67

Gene sequence:

>1353_bases
ATGACAGCCACACCGCTTGCCGCGGCCGCGATCGCCCAATTGGAGGCAGAGGGCGTCGACACCGTCATCGGCACCGTCGT
GAACCCCGCCGGACTCACCCAGGCCAAGACCGTGCCGATACGCCGGACCAACACATTCGCCAATCCTGGCCTCGGCGCCA
GTCCGGTGTGGCATACCTTCTGTATCGACCAATGCAGTATTGCATTCACCGCAGACATCAGTGTGGTCGGCGATCAACGT
CTCCGCATCGATCTGTCCGCCTTGCGCATCATCGGCGACGGGTTGGCGTGGGCGCCCGCCGGGTTCTTCGAGCAGGACGG
CACACCGGTCCCCGCCTGCAGCCGAGGAACACTGAGCCGGATCGAGGCCGCGCTTGCTGATGCCGGCATCGACGCGGTAA
TCGGCCACGAAGTCGAATTCCTCTTGGTCGACGCGGACGGCCAGCGGCTGCCTTCGACGCTGTGGGCGCAGTACGGTGTC
GCCGGGGTGCTCGAGCACGAGGCGTTCGTCCGCGATGTCAACGCCGCGGCAACGGCAGCAGGCATCGCTATCGAGCAGTT
CCATCCCGAATACGGTGCCAACCAATTCGAGATCTCGTTAGCGCCGCAGCCGCCGGTCGCGGCCGCCGATCAGCTGGTGC
TGACCCGCCTCATCATCGGCCGTACCGCCCGCCGGCACGGGTTACGCGTGAGCCTATCGCCAGCGCCCTTCGCCGGAAGT
ATCGGATCCGGTGCCCACCAACACTTCTCGCTGACTATGTCGGAAGGGATGCTGTTCTCCGGTGGGACTGGAGCAGCTGG
CATGACCTCGGCCGGGGAGGCCGCGGTGGCAGGAGTGCTTCGCGGACTACCGGACGCCCAAGGCATCCTGTGCGGATCGA
TCGTGTCCGGTCTGCGAATGCGACCCGGTAACTGGGCCGGAATCTATGCATGCTGGGGTACCGAAAACCGGGAAGCGGCG
GTGCGATTCGTCAAGGGCGGGGCTGGCAGCGCGTACGGCGGGAACGTGGAGGTGAAGGTCGTCGACCCGTCGGCCAACCC
GTATCTCGCGTCGGCGGCGATCCTCGGACTGGCACTCGACGGCATGAAGACCAAGGCGGTGTTGCCGTCGGAAACGACCG
TAGACCCGACACAGCTGTCTGACGTGGATCGTGACCGTGCCGGCATTCTGCGACTTGCTGCCGATCAGGCGGATGCAATT
GCTGTACTGGATAGTTCGAAACTGCTTCGGTGCATCCTTGGCGATCCCGTGGTAGATGCCGTGGTCGCGGTACGCCAGTT
AGAGCATGAGCGCTACGGTGACCTCGATCCTGCGCAGCTGGCCGACAAGTTCCGGATGGCTTGGAGTGTGTAA

Upstream 100 bases:

>100_bases
TCTGGGCGACCTGTCGGAATCACGCCAACTCGCGGCTACAACGTAGCGATGCTTGCCGCGCGTAGCCGCGCGAGCTGATC
CGCGCTGCAGAATGACTGCC

Downstream 100 bases:

>100_bases
CGATGGCCGACTCCGCCGGTTCGGACCTGACGCGGCACACGGCCGAAGTGCCGTTGATCGATCAGCACGTCCACGGATGC
TGGCTGACCGAGGGGAACCG

Product: putative glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFANPGLGASPVWHTFCIDQCSIAFTADISVVGDQR
LRIDLSALRIIGDGLAWAPAGFFEQDGTPVPACSRGTLSRIEAALADAGIDAVIGHEVEFLLVDADGQRLPSTLWAQYGV
AGVLEHEAFVRDVNAAATAAGIAIEQFHPEYGANQFEISLAPQPPVAAADQLVLTRLIIGRTARRHGLRVSLSPAPFAGS
IGSGAHQHFSLTMSEGMLFSGGTGAAGMTSAGEAAVAGVLRGLPDAQGILCGSIVSGLRMRPGNWAGIYACWGTENREAA
VRFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALDGMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAI
AVLDSSKLLRCILGDPVVDAVVAVRQLEHERYGDLDPAQLADKFRMAWSV

Sequences:

>Translated_450_residues
MTATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFANPGLGASPVWHTFCIDQCSIAFTADISVVGDQR
LRIDLSALRIIGDGLAWAPAGFFEQDGTPVPACSRGTLSRIEAALADAGIDAVIGHEVEFLLVDADGQRLPSTLWAQYGV
AGVLEHEAFVRDVNAAATAAGIAIEQFHPEYGANQFEISLAPQPPVAAADQLVLTRLIIGRTARRHGLRVSLSPAPFAGS
IGSGAHQHFSLTMSEGMLFSGGTGAAGMTSAGEAAVAGVLRGLPDAQGILCGSIVSGLRMRPGNWAGIYACWGTENREAA
VRFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALDGMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAI
AVLDSSKLLRCILGDPVVDAVVAVRQLEHERYGDLDPAQLADKFRMAWSV
>Mature_449_residues
TATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFANPGLGASPVWHTFCIDQCSIAFTADISVVGDQRL
RIDLSALRIIGDGLAWAPAGFFEQDGTPVPACSRGTLSRIEAALADAGIDAVIGHEVEFLLVDADGQRLPSTLWAQYGVA
GVLEHEAFVRDVNAAATAAGIAIEQFHPEYGANQFEISLAPQPPVAAADQLVLTRLIIGRTARRHGLRVSLSPAPFAGSI
GSGAHQHFSLTMSEGMLFSGGTGAAGMTSAGEAAVAGVLRGLPDAQGILCGSIVSGLRMRPGNWAGIYACWGTENREAAV
RFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALDGMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAIA
VLDSSKLLRCILGDPVVDAVVAVRQLEHERYGDLDPAQLADKFRMAWSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=256, Percent_Identity=28.90625, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081870, Length=341, Percent_Identity=26.9794721407625, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 46721; Mature: 46589

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFANPGLGASPVWHTF
CCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEECEEECCCCCCCCCCCCCHHHHH
CIDQCSIAFTADISVVGDQRLRIDLSALRIIGDGLAWAPAGFFEQDGTPVPACSRGTLSR
HHHCCCEEEEEEEEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
IEAALADAGIDAVIGHEVEFLLVDADGQRLPSTLWAQYGVAGVLEHEAFVRDVNAAATAA
HHHHHHHCCCCEEECCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GIAIEQFHPEYGANQFEISLAPQPPVAAADQLVLTRLIIGRTARRHGLRVSLSPAPFAGS
HEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
IGSGAHQHFSLTMSEGMLFSGGTGAAGMTSAGEAAVAGVLRGLPDAQGILCGSIVSGLRM
CCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCC
RPGNWAGIYACWGTENREAAVRFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALD
CCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHC
GMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAIAVLDSSKLLRCILGDPVVDA
CCCCEEECCCCCCCCCHHHCCCCCCHHCEEEEECCCCCEEEEECCHHHHHHHHCCHHHHH
VVAVRQLEHERYGDLDPAQLADKFRMAWSV
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TATPLAAAAIAQLEAEGVDTVIGTVVNPAGLTQAKTVPIRRTNTFANPGLGASPVWHTF
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEECEEECCCCCCCCCCCCCHHHHH
CIDQCSIAFTADISVVGDQRLRIDLSALRIIGDGLAWAPAGFFEQDGTPVPACSRGTLSR
HHHCCCEEEEEEEEEECCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
IEAALADAGIDAVIGHEVEFLLVDADGQRLPSTLWAQYGVAGVLEHEAFVRDVNAAATAA
HHHHHHHCCCCEEECCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GIAIEQFHPEYGANQFEISLAPQPPVAAADQLVLTRLIIGRTARRHGLRVSLSPAPFAGS
HEEHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
IGSGAHQHFSLTMSEGMLFSGGTGAAGMTSAGEAAVAGVLRGLPDAQGILCGSIVSGLRM
CCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCC
RPGNWAGIYACWGTENREAAVRFVKGGAGSAYGGNVEVKVVDPSANPYLASAAILGLALD
CCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHC
GMKTKAVLPSETTVDPTQLSDVDRDRAGILRLAADQADAIAVLDSSKLLRCILGDPVVDA
CCCCEEECCCCCCCCCHHHCCCCCCHHCEEEEECCCCCEEEEECCHHHHHHHHCCHHHHH
VVAVRQLEHERYGDLDPAQLADKFRMAWSV
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]