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The map label for this gene is gcvH [H]

Identifier: 148661632

GI number: 148661632

Start: 2081884

End: 2082288

Strand: Direct

Name: gcvH [H]

Synonym: MRA_1838

Alternate gene names: 148661632

Gene position: 2081884-2082288 (Clockwise)

Preceding gene: 148661631

Following gene: 148661633

Centisome position: 47.1

GC content: 61.48

Gene sequence:

>405_bases
GTGAGCGATATCCCGTCCGATCTGCACTACACCGCCGAACACGAGTGGATTCGCCGCAGTGGCGACGACACCGTCCGGGT
GGGGATCACCGACTATGCACAGTCGGCGCTTGGCGACGTCGTTTTCGTTCAGCTACCCGTTATCGGCACCGCGGTCACCG
CCGGCGAGACCTTCGGCGAAGTGGAATCGACGAAATCTGTGTCGGATCTCTATGCGCCCATTTCGGGTAAGGTGTCTGAG
GTCAACAGCGATCTGGACGGCACTCCGCAATTGGTGAATTCCGACCCCTACGGAGCCGGCTGGCTGCTGGACATCCAGGT
CGACAGCTCGGATGTCGCTGCCCTGGAGTCAGCTTTGACGACACTGCTCGACGCTGAGGCCTACCGCGGCACACTGACCG
AGTGA

Upstream 100 bases:

>100_bases
CGTGTGGACGTGACCGCCTTGCGGCAACCAAAACAGCACCAATACGCTCAGCCCGTCAAGTGAACTAGCCCAACTCCGAG
CCGACCAGAATAGGATTACC

Downstream 100 bases:

>100_bases
CGATTGCTAAGGTCCCTGCCAGCGTCACGTGGGAGGTCGCGGGTCTGCACGGATCCGGGCCGGGCAGGGCAATCGAGCCT
GGGATCCGCTGGGGTGCGCA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSE
VNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE

Sequences:

>Translated_134_residues
MSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSE
VNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE
>Mature_133_residues
SDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSEV
NSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=41.3223140495868, Blast_Score=95, Evalue=2e-20,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=40.495867768595, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789271, Length=132, Percent_Identity=48.4848484848485, Blast_Score=134, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=45, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=125, Percent_Identity=40.8, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=41.8604651162791, Blast_Score=112, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=43.6974789915966, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14238; Mature: 14106

Theoretical pI: Translated: 3.74; Mature: 3.74

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGE
CCCCCCCCEECHHHHHHHCCCCCEEEEEEHHHHHHHHCCEEEEEECCCCEEEECCCCCCH
VESTKSVSDLYAPISGKVSEVNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALT
HHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
TLLDAEAYRGTLTE
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGE
CCCCCCCEECHHHHHHHCCCCCEEEEEEHHHHHHHHCCEEEEEECCCCEEEECCCCCCH
VESTKSVSDLYAPISGKVSEVNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALT
HHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
TLLDAEAYRGTLTE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]