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The map label for this gene is ubiF [H]

Identifier: 148661550

GI number: 148661550

Start: 1981139

End: 1982521

Strand: Direct

Name: ubiF [H]

Synonym: MRA_1762

Alternate gene names: 148661550

Gene position: 1981139-1982521 (Clockwise)

Preceding gene: 148661547

Following gene: 148661551

Centisome position: 44.82

GC content: 65.22

Gene sequence:

>1383_bases
ATGATCGCCACGATGCCATCAATGGCCAGGAGGTCGCGACATGACAACAAGATCACCACGCCGGCAGTGGATTGCCTCAC
GATCGAACGTCTAGATTCTCCCGCGTCCGGCGCCCCTCAGGTCACCCCTTATGCTAGGGCGCTAATGGGCGAGACAACCA
CGTGCGCGATCATCGGCGGCGGCCCGGCCGGGATGGTTCTGGGCCTGCTGTTGGCGCGGGCAGGTGTGCAGGTCACCCTG
TTGGAGAAGCACGGAGACTTCCTGCGCGACTTTCGTGGCGACACGGTGCATCCGACGACGATGCGGCTACTCGACGAGCT
TGGGCTGTGGGAACGCTTTGCGGCTTTGCCCTACAGCGAGGTCCGCACGGCCACATTGCATTCGAATGGTCGCGCGGTGA
CCTACATCGACTTCGAGCGACTGCATCAGCCCTACCCCTATGTCGCAATGGTGCCGCAATGGGACCTGCTGAACCTGCTG
GCGGAGGCCGCCCAAGCGGAACCGAGCTTTACGCTGCGGATGAAAACCGAGGTGACCGGGTTGCTGCGGGAGGGCGGCAA
AGTTACGGGGGTGCGCTATCAAGGAGCCGAGGGCCCGGGTGAATTGCGGGCGGAATTGACCGTGGCGTGCGACGGCCGAT
GGTCGATCGCCCGGCACGAGGCTGGACTGAAGGCGCGTGAATTCCCGGTGAACTTTGACGTGTGGTGGTTCAAGCTGCCA
CGTGAAGGTGACGCCGAGTTCTCGTTCCTGCCGCGATTCTCCCCGGGCAAGGGGCTCGGCGTGATCCCACGCGAAGGTTA
TTTCCAGATCGCCTACCTCGGGCCCAAGGGAACCGACGCTCAGTTGCGCGAGCGAGGTATCGAGGAATTCCGTCGGGACG
TCAGCGAACTGCTGCCCGAAGCGACGGCATCGGTGGCGGCGCTAGCGTCCATGGACGAGGTCAAGCACCTCAACGTCAAG
GTGAATCGGTTGCGTCGTTGGCACATTGATGGGCTGCTGTGCATCGGCGACGCGGCGCACGCGATGTCACCGGTGGCGGG
AGTCGGCATCAACCTAGCGGTCCAAGATGCGGTCGCGGCAGCGACCATCTTGGCCGAACCGCTGCGTGAGCATCGAGTCA
GCAGCCGCCACCTGGCAGCGGTACGGCGTCGTCGCGCATTTCCCACCGCGGTGACCCAAGCGGTGCAGCGGGTGTTGCAC
CGAAGGCTGCTCGGCCCGCTGCTGCAGGGCCGGGACCCCACGCCGCCGGCGGCCCTGCTTGGCCTGGTCGAACGGCTGCC
ATGGCTCTCGGCGGTGCCCGCCTACTTTGTGGGAGTTGGAGTCCGGCCTGAGCATGCTCCGGCCTTCGCACGTCGCGGGC
CCGGCAACCGCAAAGGCCCTTGA

Upstream 100 bases:

>100_bases
GGATCGGATACGTGCTGGCGCAAGAGCGATTGAATCGTGTCGGTCATGAATTCGCTCCCATGTCGAGTCGCGGGCTTTGG
CCGCGACGCTGTCATCCAGC

Downstream 100 bases:

>100_bases
GCCGACATGCGCGCCGCCGCGAATCGGCGTCTTGGGTATAGCCCGGATAGCGCCGTTGGCGCTCATCAAGCCGGTCAGCG
GGAGCGTCGTGGTGGCAGCA

Product: hypothetical protein

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMGETTTCAIIGGGPAGMVLGLLLARAGVQVTL
LEKHGDFLRDFRGDTVHPTTMRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLHQPYPYVAMVPQWDLLNLL
AEAAQAEPSFTLRMKTEVTGLLREGGKVTGVRYQGAEGPGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLP
REGDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRRDVSELLPEATASVAALASMDEVKHLNVK
VNRLRRWHIDGLLCIGDAAHAMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFPTAVTQAVQRVLH
RRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVGVGVRPEHAPAFARRGPGNRKGP

Sequences:

>Translated_460_residues
MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMGETTTCAIIGGGPAGMVLGLLLARAGVQVTL
LEKHGDFLRDFRGDTVHPTTMRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLHQPYPYVAMVPQWDLLNLL
AEAAQAEPSFTLRMKTEVTGLLREGGKVTGVRYQGAEGPGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLP
REGDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRRDVSELLPEATASVAALASMDEVKHLNVK
VNRLRRWHIDGLLCIGDAAHAMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFPTAVTQAVQRVLH
RRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVGVGVRPEHAPAFARRGPGNRKGP
>Mature_460_residues
MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMGETTTCAIIGGGPAGMVLGLLLARAGVQVTL
LEKHGDFLRDFRGDTVHPTTMRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLHQPYPYVAMVPQWDLLNLL
AEAAQAEPSFTLRMKTEVTGLLREGGKVTGVRYQGAEGPGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLP
REGDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRRDVSELLPEATASVAALASMDEVKHLNVK
VNRLRRWHIDGLLCIGDAAHAMSPVAGVGINLAVQDAVAAATILAEPLREHRVSSRHLAAVRRRRAFPTAVTQAVQRVLH
RRLLGPLLQGRDPTPPAALLGLVERLPWLSAVPAYFVGVGVRPEHAPAFARRGPGNRKGP

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Escherichia coli, GI1786883, Length=330, Percent_Identity=24.2424242424242, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 50413; Mature: 50413

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMGETTTCAIIGG
CCCCCHHHHHHHCCCCCCCCCHHHHEEHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEECC
GPAGMVLGLLLARAGVQVTLLEKHGDFLRDFRGDTVHPTTMRLLDELGLWERFAALPYSE
CHHHHHHHHHHHHCCCEEEEEHHCCHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHCCHHH
VRTATLHSNGRAVTYIDFERLHQPYPYVAMVPQWDLLNLLAEAAQAEPSFTLRMKTEVTG
HEEEEEECCCCEEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEHHHHHH
LLREGGKVTGVRYQGAEGPGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLP
HHHCCCEEEEEEEECCCCCCCEEEEEEEEECCCEEEHHHHCCCCCCCCCCCCEEEEEECC
REGDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRRDVSELLPE
CCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ATASVAALASMDEVKHLNVKVNRLRRWHIDGLLCIGDAAHAMSPVAGVGINLAVQDAVAA
HHHHHHHHHHHHHHHHCCEEEEHHHEECCCCEEEECCHHHHHCCHHCCCCEEEHHHHHHH
ATILAEPLREHRVSSRHLAAVRRRRAFPTAVTQAVQRVLHRRLLGPLLQGRDPTPPAALL
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
GLVERLPWLSAVPAYFVGVGVRPEHAPAFARRGPGNRKGP
HHHHHCCHHHHCHHHHHCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMGETTTCAIIGG
CCCCCHHHHHHHCCCCCCCCCHHHHEEHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEECC
GPAGMVLGLLLARAGVQVTLLEKHGDFLRDFRGDTVHPTTMRLLDELGLWERFAALPYSE
CHHHHHHHHHHHHCCCEEEEEHHCCHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHCCHHH
VRTATLHSNGRAVTYIDFERLHQPYPYVAMVPQWDLLNLLAEAAQAEPSFTLRMKTEVTG
HEEEEEECCCCEEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEEHHHHHH
LLREGGKVTGVRYQGAEGPGELRAELTVACDGRWSIARHEAGLKAREFPVNFDVWWFKLP
HHHCCCEEEEEEEECCCCCCCEEEEEEEEECCCEEEHHHHCCCCCCCCCCCCEEEEEECC
REGDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRRDVSELLPE
CCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ATASVAALASMDEVKHLNVKVNRLRRWHIDGLLCIGDAAHAMSPVAGVGINLAVQDAVAA
HHHHHHHHHHHHHHHHCCEEEEHHHEECCCCEEEECCHHHHHCCHHCCCCEEEHHHHHHH
ATILAEPLREHRVSSRHLAAVRRRRAFPTAVTQAVQRVLHRRLLGPLLQGRDPTPPAALL
HHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
GLVERLPWLSAVPAYFVGVGVRPEHAPAFARRGPGNRKGP
HHHHHCCHHHHCHHHHHCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]