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The map label for this gene is hisH [H]

Identifier: 148661397

GI number: 148661397

Start: 1804182

End: 1804802

Strand: Direct

Name: hisH [H]

Synonym: MRA_1612

Alternate gene names: 148661397

Gene position: 1804182-1804802 (Clockwise)

Preceding gene: 148661396

Following gene: 148661398

Centisome position: 40.82

GC content: 64.41

Gene sequence:

>621_bases
GTGACAGCAAAATCGGTTGTAGTCCTTGACTACGGCTCAGGAAACCTGCGGTCGGCCCAACGTGCGCTGCAACGAGTAGG
CGCCGAGGTCGAAGTAACCGCCGATACCGACGCCGCAATGACCGCTGACGGACTGGTGGTGCCGGGCGTCGGTGCTTTCG
CGGCGTGCATGGCGGGCCTGCGCAAGATCAGCGGAGAGCGAATCATCGCCGAGCGGGTGGCCGCCGGCCGCCCGGTGCTG
GGGGTCTGTGTCGGTATGCAGATTCTGTTTGCTTGCGGGGTCGAATTCGGTGTGCAGACGCCAGGCTGCGGGCACTGGCC
GGGGGCGGTCATTCGACTTGAGGCCCCGGTGATTCCGCACATGGGCTGGAATGTCGTGGATTCCGCTGCGGGCAGCGCGC
TGTTCAAAGGGTTGGACGTCGACGCCCGGTTTTATTTCGTGCATTCCTATGCCGCGCAGCGATGGGAAGGCTCACCCGAC
GCGCTGCTGACCTGGGCCACATATCGGGCGCCGTTCCTCGCTGCGGTGGAGGACGGCGCATTGGCCGCCACCCAGTTTCA
TCCGGAGAAGAGTGGCGATGCCGGTGCAGCCGTACTGAGCAGCTGGGTTGATGGACTTTAA

Upstream 100 bases:

>100_bases
ATATCACCGAAGCTCAATACAAGGCCGTCGCGCGCGCGTTGCGTCAAGCGGTCGAGCCAGATCCTCGGGTGTCAGGCGTG
CCGTCCACCAAAGGTGCTCT

Downstream 100 bases:

>100_bases
AGGATACTGGTGATGCCGCTGATACTTTTGCCCGCCGTCGACGTGGTCGAGGGTCGTGCCGTGCGCCTCGTTCAAGGGAA
GGCCGGCAGCCAAACCGAGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGLRKISGERIIAERVAAGRPVL
GVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPHMGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPD
ALLTWATYRAPFLAAVEDGALAATQFHPEKSGDAGAAVLSSWVDGL

Sequences:

>Translated_206_residues
MTAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGLRKISGERIIAERVAAGRPVL
GVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPHMGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPD
ALLTWATYRAPFLAAVEDGALAATQFHPEKSGDAGAAVLSSWVDGL
>Mature_205_residues
TAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGLRKISGERIIAERVAAGRPVLG
VCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPHMGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPDA
LLTWATYRAPFLAAVEDGALAATQFHPEKSGDAGAAVLSSWVDGL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=38.8625592417062, Blast_Score=125, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=36.6666666666667, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21419; Mature: 21287

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGL
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHH
RKISGERIIAERVAAGRPVLGVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPH
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEECCCCCC
MGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPDALLTWATYRAPFLAAVEDGA
CCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCEEEEEHHCCCEEEEECCCC
LAATQFHPEKSGDAGAAVLSSWVDGL
EEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TAKSVVVLDYGSGNLRSAQRALQRVGAEVEVTADTDAAMTADGLVVPGVGAFAACMAGL
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHH
RKISGERIIAERVAAGRPVLGVCVGMQILFACGVEFGVQTPGCGHWPGAVIRLEAPVIPH
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEECCCCCC
MGWNVVDSAAGSALFKGLDVDARFYFVHSYAAQRWEGSPDALLTWATYRAPFLAAVEDGA
CCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCEEEEEHHCCCEEEEECCCC
LAATQFHPEKSGDAGAAVLSSWVDGL
EEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]