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The map label for this gene is bioD [H]

Identifier: 148661366

GI number: 148661366

Start: 1779376

End: 1780056

Strand: Direct

Name: bioD [H]

Synonym: MRA_1582

Alternate gene names: 148661366

Gene position: 1779376-1780056 (Clockwise)

Preceding gene: 148661365

Following gene: 148661367

Centisome position: 40.26

GC content: 72.54

Gene sequence:

>681_bases
TTGACGATCCTGGTCGTCACCGGGACCGGCACGGGGGTCGGCAAGACGGTCGTCTGCGCGGCGCTGGCGTCGGCCGCACG
TCAGGCCGGCATCGACGTGGCGGTGTGCAAGCCCGTTCAGACCGGCACCGCCCGCGGTGACGACGACCTCGCCGAGGTCG
GCCGGTTGGCCGGGGTGACCCAGCTGGCCGGCTTGGCGCGATATCCGCAGCCGATGGCCCCGGCCGCCGCCGCCGAACAC
GCCGGGATGGCGTTGCCCGCCCGCGATCAGATCGTGCGGCTGATCGCAGACCTGGACCGTCCCGGGCGGTTGACCCTCGT
CGAGGGGGCGGGCGGGCTGCTGGTCGAACTCGCCGAGCCGGGCGTCACGCTGCGCGATGTCGCCGTCGACGTGGCCGCCG
CGGCTTTGGTGGTGGTCACCGCGGACCTGGGCACCCTCAACCACACCAAGTTGACGTTGGAAGCGCTTGCTGCACAACAG
GTTTCATGTGCAGGGCTGGTGATCGGCAGCTGGCCGGACCCGCCCGGGTTGGTGGCAGCCTCGAATCGGTCCGCGCTGGC
GCGCATTGCTATGGTGCGGGCCGCTCTGCCCGCCGGGGCCGCGTCGCTGGATGCCGGGGACTTCGCGGCGATGAGCGCGG
CGGCGTTCGACCGCAACTGGGTTGCCGGGCTGGTCGGCTGA

Upstream 100 bases:

>100_bases
GTACGTCGCGGCTGCGGCTGACCGCGCGCGCATCGCTGAACGCCGGCGAGCTCGAGCTGGCCCGGCGGGTGCTGACGGAT
GTTCTCGCCGTGGCGCGCCG

Downstream 100 bases:

>100_bases
TGGTGCATTCGATCGAGCTGGTCTTCGACAGCGATACCGAGGCGGCGATCCGGCGCATCTGGGCGGGGTTGGCCGCCGCC
GGCATACCCAGCCAGGCGCC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTILVVTGTGTGVGKTVVCAALASAARQAGIDVAVCKPVQTGTARGDDDLAEVGRLAGVTQLAGLARYPQPMAPAAAAEH
AGMALPARDQIVRLIADLDRPGRLTLVEGAGGLLVELAEPGVTLRDVAVDVAAAALVVVTADLGTLNHTKLTLEALAAQQ
VSCAGLVIGSWPDPPGLVAASNRSALARIAMVRAALPAGAASLDAGDFAAMSAAAFDRNWVAGLVG

Sequences:

>Translated_226_residues
MTILVVTGTGTGVGKTVVCAALASAARQAGIDVAVCKPVQTGTARGDDDLAEVGRLAGVTQLAGLARYPQPMAPAAAAEH
AGMALPARDQIVRLIADLDRPGRLTLVEGAGGLLVELAEPGVTLRDVAVDVAAAALVVVTADLGTLNHTKLTLEALAAQQ
VSCAGLVIGSWPDPPGLVAASNRSALARIAMVRAALPAGAASLDAGDFAAMSAAAFDRNWVAGLVG
>Mature_225_residues
TILVVTGTGTGVGKTVVCAALASAARQAGIDVAVCKPVQTGTARGDDDLAEVGRLAGVTQLAGLARYPQPMAPAAAAEHA
GMALPARDQIVRLIADLDRPGRLTLVEGAGGLLVELAEPGVTLRDVAVDVAAAALVVVTADLGTLNHTKLTLEALAAQQV
SCAGLVIGSWPDPPGLVAASNRSALARIAMVRAALPAGAASLDAGDFAAMSAAAFDRNWVAGLVG

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=198, Percent_Identity=31.3131313131313, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472 [H]

Pfam domain/function: NA

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 22456; Mature: 22325

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILVVTGTGTGVGKTVVCAALASAARQAGIDVAVCKPVQTGTARGDDDLAEVGRLAGVT
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
QLAGLARYPQPMAPAAAAEHAGMALPARDQIVRLIADLDRPGRLTLVEGAGGLLVELAEP
HHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCC
GVTLRDVAVDVAAAALVVVTADLGTLNHTKLTLEALAAQQVSCAGLVIGSWPDPPGLVAA
CCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEE
SNRSALARIAMVRAALPAGAASLDAGDFAAMSAAAFDRNWVAGLVG
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TILVVTGTGTGVGKTVVCAALASAARQAGIDVAVCKPVQTGTARGDDDLAEVGRLAGVT
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
QLAGLARYPQPMAPAAAAEHAGMALPARDQIVRLIADLDRPGRLTLVEGAGGLLVELAEP
HHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCC
GVTLRDVAVDVAAAALVVVTADLGTLNHTKLTLEALAAQQVSCAGLVIGSWPDPPGLVAA
CCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEE
SNRSALARIAMVRAALPAGAASLDAGDFAAMSAAAFDRNWVAGLVG
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]