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The map label for this gene is ansA [H]

Identifier: 148661334

GI number: 148661334

Start: 1742729

End: 1743709

Strand: Reverse

Name: ansA [H]

Synonym: MRA_1550

Alternate gene names: 148661334

Gene position: 1743709-1742729 (Counterclockwise)

Preceding gene: 148661337

Following gene: 148661328

Centisome position: 39.45

GC content: 69.62

Gene sequence:

>981_bases
ATGGGAGCAAACCACGTGCGCAATGATCCCATCATGGCCCGCCTCACCGTCATCACTACTGGAGGGACAATCTCGACCAC
CGCCGGCCCCGATGGGGTGCTACGGCCAACCCATTGCGGGGCGACGCTGATCGCCGGTCTCGACATGGACTCCGACATCG
AAGTAGTCGACCTCATGGCGCTGGACAGCTCAAAGTTGACGCCGGCCGATTGGGACCGGATAGGCGCTGCAGTGCAGGAG
GCATTCCGCGGCGGCGCAGATGGGGTGGTCATCACTCACGGCACCGATACGCTCGAAGAGACCGCTCTGTGGCTGGACCT
CACCTATGCGGGTAGCCGCCCGGTCGTGTTGACCGGGGCCATGCTCAGCGCCGATGCCCCCGGCGCCGATGGCCCGGCCA
ATCTGCGCGACGCGCTTGCGGTCGCCGCCGACCCAGCCGCCCGCGACCTCGGGGTGCTGGTGAGTTTCGGCGGCCGGGTG
TTACAGCCTTTGGGCCTGCACAAGGTGGCCAACCCGGACCTGTGCGGATTCGCCGGCGAGTCGCTTGGCTTCACGTCGGG
CGGGGTGCGGCTGACGCGCACCAAGACGCGGCCCTATCTCGGCGATCTGGGCGCTGCCGTGGCGCCACGAGTCGACATCG
TCGCCGTCTATCCGGGAAGTGACGCGGTGGCGATGGACGCCTGTGTGGCAGCCGGCGCGCGCGCCGTGGTGCTGGAGGCG
CTGGGCTCGGGCAATGCCGGGGCCGCCGTGATCGAGGGAGTCCGTCGCCACTGTCGAGACGGGAGCGATCCGGTGGTGAT
CGCGGTGTCTACCCGGGTCGCCGGCGCGCGGGTCGGCGCAGGCTATGGTCCCGGGCACGACCTGGTGGAGGCTGGCGCGG
TGATGGTACCGCGACTGCCGCCGTCCCAGGCCCGGGTGCTGCTAATGGCCGCGCTGGCGGCCAACTCACCGGTCGCCGAC
GTCATCGACCGTTGGGGCTGA

Upstream 100 bases:

>100_bases
CCTCGGTCGAGGTCAGCGGATCAGCCGATCCTGTTGGTTCGTCAGGCACACCATCATCATCCCCTAGGGCCGATATGGCC
CGCCCAGACCCCGCGGCCGG

Downstream 100 bases:

>100_bases
GGCGCCGGCGGACCCCTCGACCGATAGCTGGCCGATGTAGTCCGGCCAGTCCAAGCTGTCAAGCGGGCTGGCGTTGCTGA
TGTCGCCGGTGTCGACGGGG

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGANHVRNDPIMARLTVITTGGTISTTAGPDGVLRPTHCGATLIAGLDMDSDIEVVDLMALDSSKLTPADWDRIGAAVQE
AFRGGADGVVITHGTDTLEETALWLDLTYAGSRPVVLTGAMLSADAPGADGPANLRDALAVAADPAARDLGVLVSFGGRV
LQPLGLHKVANPDLCGFAGESLGFTSGGVRLTRTKTRPYLGDLGAAVAPRVDIVAVYPGSDAVAMDACVAAGARAVVLEA
LGSGNAGAAVIEGVRRHCRDGSDPVVIAVSTRVAGARVGAGYGPGHDLVEAGAVMVPRLPPSQARVLLMAALAANSPVAD
VIDRWG

Sequences:

>Translated_326_residues
MGANHVRNDPIMARLTVITTGGTISTTAGPDGVLRPTHCGATLIAGLDMDSDIEVVDLMALDSSKLTPADWDRIGAAVQE
AFRGGADGVVITHGTDTLEETALWLDLTYAGSRPVVLTGAMLSADAPGADGPANLRDALAVAADPAARDLGVLVSFGGRV
LQPLGLHKVANPDLCGFAGESLGFTSGGVRLTRTKTRPYLGDLGAAVAPRVDIVAVYPGSDAVAMDACVAAGARAVVLEA
LGSGNAGAAVIEGVRRHCRDGSDPVVIAVSTRVAGARVGAGYGPGHDLVEAGAVMVPRLPPSQARVLLMAALAANSPVAD
VIDRWG
>Mature_325_residues
GANHVRNDPIMARLTVITTGGTISTTAGPDGVLRPTHCGATLIAGLDMDSDIEVVDLMALDSSKLTPADWDRIGAAVQEA
FRGGADGVVITHGTDTLEETALWLDLTYAGSRPVVLTGAMLSADAPGADGPANLRDALAVAADPAARDLGVLVSFGGRVL
QPLGLHKVANPDLCGFAGESLGFTSGGVRLTRTKTRPYLGDLGAAVAPRVDIVAVYPGSDAVAMDACVAAGARAVVLEAL
GSGNAGAAVIEGVRRHCRDGSDPVVIAVSTRVAGARVGAGYGPGHDLVEAGAVMVPRLPPSQARVLLMAALAANSPVADV
IDRWG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=236, Percent_Identity=35.1694915254237, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788065, Length=243, Percent_Identity=28.3950617283951, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320527, Length=327, Percent_Identity=27.5229357798165, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323189, Length=242, Percent_Identity=29.7520661157025, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=242, Percent_Identity=29.7520661157025, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=242, Percent_Identity=29.7520661157025, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=242, Percent_Identity=29.7520661157025, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645530, Length=285, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 32947; Mature: 32816

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGANHVRNDPIMARLTVITTGGTISTTAGPDGVLRPTHCGATLIAGLDMDSDIEVVDLMA
CCCCCCCCCCEEEEEEEEEECCEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEE
LDSSKLTPADWDRIGAAVQEAFRGGADGVVITHGTDTLEETALWLDLTYAGSRPVVLTGA
ECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEEEEEEECCCCCEEEEEE
MLSADAPGADGPANLRDALAVAADPAARDLGVLVSFGGRVLQPLGLHKVANPDLCGFAGE
EEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHCCCCCHHCCCCCCCCCCCC
SLGFTSGGVRLTRTKTRPYLGDLGAAVAPRVDIVAVYPGSDAVAMDACVAAGARAVVLEA
CCCCCCCCEEEEEECCCCCHHHCCHHHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEH
LGSGNAGAAVIEGVRRHCRDGSDPVVIAVSTRVAGARVGAGYGPGHDLVEAGAVMVPRLP
HCCCCCCHHHHHHHHHHCCCCCCCEEEEEECEECCEECCCCCCCCHHHHHCCCEEECCCC
PSQARVLLMAALAANSPVADVIDRWG
CHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
GANHVRNDPIMARLTVITTGGTISTTAGPDGVLRPTHCGATLIAGLDMDSDIEVVDLMA
CCCCCCCCCEEEEEEEEEECCEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEE
LDSSKLTPADWDRIGAAVQEAFRGGADGVVITHGTDTLEETALWLDLTYAGSRPVVLTGA
ECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEEEEEEECCCCCEEEEEE
MLSADAPGADGPANLRDALAVAADPAARDLGVLVSFGGRVLQPLGLHKVANPDLCGFAGE
EEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHCCCCCHHCCCCCCCCCCCC
SLGFTSGGVRLTRTKTRPYLGDLGAAVAPRVDIVAVYPGSDAVAMDACVAAGARAVVLEA
CCCCCCCCEEEEEECCCCCHHHCCHHHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEH
LGSGNAGAAVIEGVRRHCRDGSDPVVIAVSTRVAGARVGAGYGPGHDLVEAGAVMVPRLP
HCCCCCCHHHHHHHHHHCCCCCCCEEEEEECEECCEECCCCCCCCHHHHHCCCEEECCCC
PSQARVLLMAALAANSPVADVIDRWG
CHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]