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The map label for this gene is fadD25

Identifier: 148661317

GI number: 148661317

Start: 1713819

End: 1715570

Strand: Direct

Name: fadD25

Synonym: MRA_1533

Alternate gene names: 148661317

Gene position: 1713819-1715570 (Clockwise)

Preceding gene: 148661316

Following gene: 148661319

Centisome position: 38.77

GC content: 59.99

Gene sequence:

>1752_bases
ATGTCCGTGGTCGAATCCTCTCTTCCTGGTGTGCTGCGTGAACGCGCCAGTTTTCAGCCCAACGACAAAGCGCTCACCTT
TATCGATTACGAGCGGTCCTGGGATGGTGTTGAAGAAACTCTGACGTGGTCGCAGTTATATCGGCGAACGCTTAACCTCG
CCGCACAGCTAAGAGAACATGGGTCGACCGGCGATCGGGCATTAATTCTGGCGCCACAAAGCCTCGACTATGTCGTTAGC
TTTATTGCCTCGCTGCAGGCCGGAATTGTCGCGGTTCCGCTTTCGATTCCCCAGGGTGGTGCCCACGACGAGCGCACCGT
TTCCGTGTTCGCCGATACCGCACCGGCGATCGTTCTCACGGCGTCCTCGGTCGTCGACAATGTCGTCGAATACGTCCAGC
CGCAGCCCGGCCAAAACGCACCGGCGGTGATCGAAGTCGATCGGCTGGATCTTGATGCTCGGCCGAGCTCCGGTTCTCGT
TCTGCCGCTCACGGCCATCCGGATATCTTGTACTTGCAGTACACCTCGGGTTCCACGCGCACGCCGGCCGGTGTCATGGT
CTCGAATAAGAATCTTTTCGCCAATTTCGAACAAATTATGACCAGTTACTACGGCGTCTATGGCAAGGTCGCCCCGCCAG
GCTCCACCGTGGTGTCGTGGTTGCCGTTCTATCACGACATGGGTTTCGTCTTGGGACTGATATTGCCGATTCTGGCTGGC
ATCCCCGCCGTGCTGACCAGCCCGATCGGTTTCCTGCAGCGCCCGGCTCGCTGGATACAGATGTTGGCAAGCAACACTCT
TGCGTTTACCGCCGCGCCGAACTTCGCATTCGATCTGGCGTCTCGTAAGACCAAAGACGAGGACATGGAGGGCCTCGATC
TCGGTGGCGTACACGGCATCCTCAACGGCAGCGAACGGGTGCAGCCGGTGACGCTGAAGCGCTTCATCGACCGGTTCGCC
CCGTTCAATCTTGACCCCAAGGCGATACGTCCGTCGTACGGAATGGCAGAGGCCACGGTATATGTGGCCACCCGCAAGGC
GGGTCAACCGCCAAAGATAGTGCAATTCGATCCCCAGAAGCTGCCGGACGGCCAAGCTGAGCGGACCGAAAGCGACGGCG
GCACACCGCTGGTCAGCTACGGCATCGTCGACACCCAGCTGGTGCGCATCGTCGACCCGGACACCGGCATCGAGCGCCCC
GCGGGAACGATCGGTGAGATTTGGGTGCACGGCGACAACGTCGCCATCGGCTATTGGCAGAAACCCGAGGCGACCGAACG
CACCTTTAGCGCAACGATCGTCAATCCCTCCGAAGGCACACCCGCAGGACCATGGCTGCGGACGGGAGATTCGGGTTTCC
TCTCCGAGGGTGAGCTGTTCATCATGGGGCGCATCAAGGACCTCTTGATCGTGTACGGGCGCAACCACTCTCCCGACGAT
ATCGAGGCGACGATTCAGACGATCAGTCCGGGCCGCTGTGCGGCGATCGCTGTTTCCGAGCATGGTGCTGAGAAGCTGGT
TGCCATTATTGAACTCAAGAAGAAGGACGAGTCCGACGACGAGGCGGCGGAACGACTGGGTTTCGTGAAACGCGAAGTGA
CCTCGGCAATCTCGAAGTCGCACGGGTTGAGCGTGGCGGATCTTGTGCTCGTCTCCCCGGGCTCAATCCCAATCACCACC
AGCGGCAAGATCCGGCGAGCACAGTGTGTGGAGCTGTACCGTCAGGACGAGTTCACTCGCCTGGACGCATAG

Upstream 100 bases:

>100_bases
CTGATGTCCTCCAGGCTCTGGTCGGGATGAGCTAGTGCGGTTCCCGAACTCGGCATCTTCGTCAGTCCTGGAGAGAAACA
ACACCAGCGAAGGTAGTGTG

Downstream 100 bases:

>100_bases
CACCCACAGGCGAGGCTCCCGCAATGGGGCGCAATGGGGATCGTCACACCAGTAGCACCAGCCCCTGGAGGGGCAACAGG
GGAAAACTGAGTTGAGCGCC

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: Acyl-CoA synthetase

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MSVVESSLPGVLRERASFQPNDKALTFIDYERSWDGVEETLTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVS
FIASLQAGIVAVPLSIPQGGAHDERTVSVFADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVIEVDRLDLDARPSSGSR
SAAHGHPDILYLQYTSGSTRTPAGVMVSNKNLFANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAG
IPAVLTSPIGFLQRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEGLDLGGVHGILNGSERVQPVTLKRFIDRFA
PFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQKLPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERP
AGTIGEIWVHGDNVAIGYWQKPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGELFIMGRIKDLLIVYGRNHSPDD
IEATIQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKSHGLSVADLVLVSPGSIPITT
SGKIRRAQCVELYRQDEFTRLDA

Sequences:

>Translated_583_residues
MSVVESSLPGVLRERASFQPNDKALTFIDYERSWDGVEETLTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVS
FIASLQAGIVAVPLSIPQGGAHDERTVSVFADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVIEVDRLDLDARPSSGSR
SAAHGHPDILYLQYTSGSTRTPAGVMVSNKNLFANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAG
IPAVLTSPIGFLQRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEGLDLGGVHGILNGSERVQPVTLKRFIDRFA
PFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQKLPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERP
AGTIGEIWVHGDNVAIGYWQKPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGELFIMGRIKDLLIVYGRNHSPDD
IEATIQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKSHGLSVADLVLVSPGSIPITT
SGKIRRAQCVELYRQDEFTRLDA
>Mature_582_residues
SVVESSLPGVLRERASFQPNDKALTFIDYERSWDGVEETLTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSF
IASLQAGIVAVPLSIPQGGAHDERTVSVFADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVIEVDRLDLDARPSSGSRS
AAHGHPDILYLQYTSGSTRTPAGVMVSNKNLFANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGI
PAVLTSPIGFLQRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEGLDLGGVHGILNGSERVQPVTLKRFIDRFAP
FNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQKLPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERPA
GTIGEIWVHGDNVAIGYWQKPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGELFIMGRIKDLLIVYGRNHSPDDI
EATIQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKSHGLSVADLVLVSPGSIPITTS
GKIRRAQCVELYRQDEFTRLDA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI55749758, Length=573, Percent_Identity=25.4799301919721, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI44888818, Length=607, Percent_Identity=24.0527182866557, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI45827692, Length=604, Percent_Identity=23.841059602649, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI225735629, Length=604, Percent_Identity=23.841059602649, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI156151445, Length=564, Percent_Identity=25.177304964539, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI225735625, Length=612, Percent_Identity=23.8562091503268, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788107, Length=576, Percent_Identity=26.3888888888889, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=562, Percent_Identity=26.1565836298932, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI193202679, Length=625, Percent_Identity=25.44, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=323, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=413, Percent_Identity=24.6973365617433, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24654656, Length=475, Percent_Identity=22.1052631578947, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355181, Length=567, Percent_Identity=22.3985890652557, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581924, Length=579, Percent_Identity=23.4887737478411, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FAD25_MYCTU (Q50586)

Other databases:

- EMBL:   BX842576
- EMBL:   AE000516
- PIR:   A70723
- RefSeq:   NP_216037.1
- RefSeq:   NP_336025.1
- ProteinModelPortal:   Q50586
- EnsemblBacteria:   EBMYCT00000000642
- EnsemblBacteria:   EBMYCT00000069888
- GeneID:   886448
- GeneID:   924387
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1572
- KEGG:   mtu:Rv1521
- TIGR:   MT1572
- TubercuList:   Rv1521
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   TADENID
- ProtClustDB:   PRK05850
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3

Molecular weight: Translated: 63142; Mature: 63011

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e317624)-; HASH(0x1e7cbd40)-; HASH(0x1e442a28)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVESSLPGVLRERASFQPNDKALTFIDYERSWDGVEETLTWSQLYRRTLNLAAQLREH
CCCHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGGAHDERTVSVFADTAPAIVLT
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEE
ASSVVDNVVEYVQPQPGQNAPAVIEVDRLDLDARPSSGSRSAAHGHPDILYLQYTSGSTR
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
TPAGVMVSNKNLFANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAG
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IPAVLTSPIGFLQRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEGLDLGGVHGI
CHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEE
LNGSERVQPVTLKRFIDRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQK
CCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEEEEECCCCCCCCEEEECCCC
LPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYWQ
CCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEECC
KPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGELFIMGRIKDLLIVYGRNHSPDD
CCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCCEEEEEEECEEEEEECCCCCCCH
IEATIQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKS
HHHHHEECCCCCEEEEEECCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
HGLSVADLVLVSPGSIPITTSGKIRRAQCVELYRQDEFTRLDA
CCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCHHCCCH
>Mature Secondary Structure 
SVVESSLPGVLRERASFQPNDKALTFIDYERSWDGVEETLTWSQLYRRTLNLAAQLREH
CCHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGGAHDERTVSVFADTAPAIVLT
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEE
ASSVVDNVVEYVQPQPGQNAPAVIEVDRLDLDARPSSGSRSAAHGHPDILYLQYTSGSTR
HHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
TPAGVMVSNKNLFANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAG
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IPAVLTSPIGFLQRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEGLDLGGVHGI
CHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEE
LNGSERVQPVTLKRFIDRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQK
CCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEEEEECCCCCCCCEEEECCCC
LPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYWQ
CCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEECC
KPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGELFIMGRIKDLLIVYGRNHSPDD
CCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCCEEEEEEECEEEEEECCCCCCCH
IEATIQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKS
HHHHHEECCCCCEEEEEECCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
HGLSVADLVLVSPGSIPITTSGKIRRAQCVELYRQDEFTRLDA
CCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHCCCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036