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The map label for this gene is gmd [C]

Identifier: 148661304

GI number: 148661304

Start: 1701383

End: 1701745

Strand: Direct

Name: gmd [C]

Synonym: MRA_1520

Alternate gene names: 148661304

Gene position: 1701383-1701745 (Clockwise)

Preceding gene: 148661297

Following gene: 148661305

Centisome position: 38.49

GC content: 58.95

Gene sequence:

>363_bases
GTGAAGCGAGCGCTCATCACCGGAATCACAGGACCGGACGGCTCGTATCTCGCTAAGCTCCCGCTGAAGGGATATGTGGC
CGCTGGTAGCCCGGCCGAGGTCTATTTCTGCTGGGCGACACGGAATTATCGCGAATTGTATGGGTTGCTCGCGGTCAACA
GCATCTGGTTCAATCACGAATCACCGCGTCACGGCGAGACATTCATGACTCGTAATCCTGCACCATATCGCGGTCGGCAA
CGAGGCGCTGATCGATGCGCAGACGCTGATGCGCCGGCCCACCCGGATAGGTATCAGTATTGGGGCGTTCCGGCCAGCGT
ACGAGGCGTGATCGACCGCGCAATGGGTGTTTGCGTTGAGTAA

Upstream 100 bases:

>100_bases
GTTGCCGAAAGGGCGCTGGGCACCGACATCTAGGATGAACCCACAGCCACGCCCCGACGTTATGCCATGGCGAAGAGCGA
CCGGCAGGAGCGGGAACCCA

Downstream 100 bases:

>100_bases
TAATCTGAACCGTGTGAACGCATGCATGGATGGATTCCTTGCCCGTATCCGCTCACATGTTGATGCGCACGCGCCAGAAT
TGCGTTCACTGTTCGATACG

Product: hypothetical protein

Products: GDP-4-dehydro-6-deoxy-D-mannose; H2O

Alternate protein names: None

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MKRALITGITGPDGSYLAKLPLKGYVAAGSPAEVYFCWATRNYRELYGLLAVNSIWFNHESPRHGETFMTRNPAPYRGRQ
RGADRCADADAPAHPDRYQYWGVPASVRGVIDRAMGVCVE

Sequences:

>Translated_120_residues
MKRALITGITGPDGSYLAKLPLKGYVAAGSPAEVYFCWATRNYRELYGLLAVNSIWFNHESPRHGETFMTRNPAPYRGRQ
RGADRCADADAPAHPDRYQYWGVPASVRGVIDRAMGVCVE
>Mature_120_residues
MKRALITGITGPDGSYLAKLPLKGYVAAGSPAEVYFCWATRNYRELYGLLAVNSIWFNHESPRHGETFMTRNPAPYRGRQ
RGADRCADADAPAHPDRYQYWGVPASVRGVIDRAMGVCVE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.47

Molecular weight: Translated: 13349; Mature: 13349

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRALITGITGPDGSYLAKLPLKGYVAAGSPAEVYFCWATRNYRELYGLLAVNSIWFNHE
CCCEEEEECCCCCCCEEEECCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHEEEECCC
SPRHGETFMTRNPAPYRGRQRGADRCADADAPAHPDRYQYWGVPASVRGVIDRAMGVCVE
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRALITGITGPDGSYLAKLPLKGYVAAGSPAEVYFCWATRNYRELYGLLAVNSIWFNHE
CCCEEEEECCCCCCCEEEECCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHEEEECCC
SPRHGETFMTRNPAPYRGRQRGADRCADADAPAHPDRYQYWGVPASVRGVIDRAMGVCVE
CCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADP+; NADPH+; Pyridine nucleotides [C]

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.55 {GDPmannose}} 0.26 {GDPmannose}} 0.222 {GDPmannose}} [C]

Substrates: GDPmannose

Specific reaction: GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: GDP-D- glucose; GDP-L-fucose; p-Chloromercuriphenyl sulfonate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA