Click here to switch to the map view.

The map label for this gene is echA12 [H]

Identifier: 148661264

GI number: 148661264

Start: 1661280

End: 1662137

Strand: Direct

Name: echA12 [H]

Synonym: MRA_1481

Alternate gene names: 148661264

Gene position: 1661280-1662137 (Clockwise)

Preceding gene: 148661263

Following gene: 148661265

Centisome position: 37.59

GC content: 64.92

Gene sequence:

>858_bases
GTGCCCCACCGCTGCGCGGCGCAAGTCGTCGCCGGGTACCGTTCAACGGTGAGTTTGGTCCTCGTCGAACACCCGCGGCC
CGAGATCGCGCAGATTACCCTCAACCGGCCGGAGCGGATGAACTCCATGGCATTCGATGTCATGGTGCCGCTCAAAGAGG
CCTTAGCGCAGGTCAGCTACGACAACTCGGTGCGGGTGGTGGTGCTGACCGGCGCGGGTCGAGGGTTTTCTCCGGGTGCG
GATCACAAGTCGGCGGGGGTGGTGCCGCACGTCGAGAACTTGACTCGGCCCACCTACGCGCTGCGTTCGATGGAGCTCCT
CGATGACGTCATCTTAATGCTGCGACGGCTGCACCAGCCGGTGATCGCCGCGGTCAACGGCCCCGCCATCGGTGGTGGGC
TGTGCCTGGCACTGGCTGCAGACATTCGGGTGGCCTCGAGTAGCGCCTACTTCCGGGCCGCCGGTATCAACAACGGGCTG
ACCGCCAGCGAATTGGGGCTGAGCTACCTGTTGCCCAGGGCCATTGGATCCTCACGTGCGTTCGAGATCATGTTGACCGG
TCGCGACGTCAGCGCCGAGGAAGCCGAGAGGATCGGGCTGGTATCCCGTCAGGTACCCGATGAACAGCTGCTAGATGCCT
GCTACGCGATCGCCGCACGGATGGCGGGATTCTCGCGGCCGGGAATTGAGTTGACCAAACGTACGCTGTGGAGTGGACTG
GACGCCGCCAGTCTGGAGGCGCACATGCAGGCCGAGGGCTTGGGGCAGCTCTTCGTCCGGCTGCTCACCGCCAACTTCGA
AGAAGCGGTTGCCGCACGGGCCGAGCAGCGGGCGCCGGTGTTCACCGATGACACGTAA

Upstream 100 bases:

>100_bases
CCTGGTGCAGCAGCTCAAGGCCTACGAGGTGGAGGCCGGCGAAGCCACCACCCAGAACGGGCGAGCCCAACAAGCCTGAC
CGGGCGCCAGGCGCCCGGCT

Downstream 100 bases:

>100_bases
CAGCGCCCAAGACAACCGACGACCAGGGAGCGAATGTGATCACAGCTACGGACCTCGAGGTCCGCGCTGGCGCGCGCATC
CTGCTCGCACCCGACGGCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSYDNSVRVVVLTGAGRGFSPGA
DHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQPVIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGL
TASELGLSYLLPRAIGSSRAFEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT

Sequences:

>Translated_285_residues
MPHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSYDNSVRVVVLTGAGRGFSPGA
DHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQPVIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGL
TASELGLSYLLPRAIGSSRAFEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT
>Mature_284_residues
PHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSYDNSVRVVVLTGAGRGFSPGAD
HKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQPVIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGLT
ASELGLSYLLPRAIGSSRAFEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGLD
AASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=285, Percent_Identity=29.8245614035088, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.9548872180451, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI31542718, Length=269, Percent_Identity=29.368029739777, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=29.5698924731183, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=28.8372093023256, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=28.8372093023256, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=28.8372093023256, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757966, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538581, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI25453481, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538573, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI116235487, Length=163, Percent_Identity=28.2208588957055, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=34.8484848484849, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=32.0158102766798, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788597, Length=179, Percent_Identity=32.9608938547486, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI221142681, Length=196, Percent_Identity=33.6734693877551, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=31.8840579710145, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=36.078431372549, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=31.140350877193, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=281, Percent_Identity=31.3167259786477, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=32.512315270936, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=34.1584158415842, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=276, Percent_Identity=26.0869565217391, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=223, Percent_Identity=28.2511210762332, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=188, Percent_Identity=27.1276595744681, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=256, Percent_Identity=25.390625, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=289, Percent_Identity=32.1799307958478, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653477, Length=289, Percent_Identity=32.1799307958478, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920382, Length=283, Percent_Identity=30.3886925795053, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922422, Length=275, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=280, Percent_Identity=32.8571428571429, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=30.9417040358744, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654903, Length=171, Percent_Identity=30.9941520467836, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=33.1707317073171, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 30576; Mature: 30444

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSY
CCCHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
DNSVRVVVLTGAGRGFSPGADHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCH
VIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGLTASELGLSYLLPRAIGSSRA
HEEECCCCCCCHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCE
FEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
EEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSY
CCHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
DNSVRVVVLTGAGRGFSPGADHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCH
VIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGLTASELGLSYLLPRAIGSSRA
HEEECCCCCCCHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCE
FEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
EEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]