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The map label for this gene is prpD2 [H]

Identifier: 148660916

GI number: 148660916

Start: 1255865

End: 1257445

Strand: Direct

Name: prpD2 [H]

Synonym: MRA_1139

Alternate gene names: 148660916

Gene position: 1255865-1257445 (Clockwise)

Preceding gene: 148660911

Following gene: 148660917

Centisome position: 28.41

GC content: 65.59

Gene sequence:

>1581_bases
GTGCCGGATCAGGACACAAAAGTACGCTTTTTCAGGGTCTTTTGTTGGTGTCCTGTGCTGCGTATGGTGCGGATTATGTT
GATGCATGCGGTCCGGGCGTGGCGCAGCGCCGACGATTTCCCGTGCACCGAGCACATGGCCTACAAGATCGCCCAGGTGG
CTGCCGATCCGGTTGACGTCGACCCGGAGGTAGCGGACATGGTGTGCAACCGCATCATCGACAACGCTGCGGTGAGCGCC
GCATCAATGGTGCGCAGACCGGTCACCGTGGCCCGCCACCAGGCACTGGCGCATCCGGTGCGACACGGGGCGAAGGTATT
TGGCGTCGAGGGCAGCTACTCGGCGGACTGGGCGGCCTGGGCCAACGGCGTCGCCGCGCGTGAACTTGACTTTCACGACA
CGTTTCTGGCCGCCGACTATTCGCACCCGGCGGACAACATACCCCCACTGGTGGCGGTCGCCCAGCAGCTCGGCGTGTGC
GGCGCGGAGCTGATCCGCGGTCTGGTAACCGCCTATGAGATCCACATCGACCTAACCCGCGGAATCTGCTTGCACGAGCA
CAAGATCGACCATGTCGCCCACCTGGGCCCGGCGGTGGCCGCCGGCATCGGGACCATGCTGCGGCTCGACCAAGAGACCA
TCTACCACGCGATCGGCCAGGCCCTGCATCTGACCACCAGCACCCGTCAATCCCGCAAGGGCGCCATCTCCAGCTGGAAG
GCGTTCGCGCCGGCGCATGCCGGCAAGGTCGGCATCGAGGCGGTCGATCGGGCGATGCGCGGCGAGGGCTCACCGGCTCC
GATCTGGGAGGGCGAGGACGGGGTGATCGCCTGGCTGCTGGCCGGACCCGAGCACACCTACCGGGTGCCGTTGCCCGCAC
CTGGTGAACCCAAGCGCGCCATTCTGGACAGCTACACCAAGCAACACTCCGCGGAGTACCAGAGCCAGGCGCCGATCGAC
CTGGCCTGCCGGCTACGTGAGCGTATCGGCGATCTCGACCAGATCGCGTCGATCGTGCTGCACACCAGCCACCACACCCA
TGTAGTGATCGGAACGGGATCCGGCGATCCGCAGAAGTTCGACCCGGACGCGTCACGCGAAACCCTCGACCACTCGCTGC
CCTACATCTTCGCCGTGGCACTGCAGGACGGCTGCTGGCACCACGAGCGCTCCTACGCGCCCGAGCGGGCGCGCCGTTCC
GACACGGTGGCACTGTGGCACAAGATTTCCACCGTCGAGGATCCCGAGTGGACCCGCCGCTATCACTGCGCCGATCCGGC
CAAAAAGGCGTTCGGGGCGCGCGCGGAGGTGACGCTGCACAGCGGTGAAGTGATCGTGGACGAACTGGCGGTGGCCGACG
CCCATCCGCTGGGCACCCGGCCGTTCGAGCGCAAGCAGTACGTAGAGAAGTTCACCGAGCTCGCCGATGGTGTAGTGGAA
CCCGTTGAACAGCAACGGTTCCTGGCCGTAGTAGAGAGTCTCGCCGATCTCGAGAGCGGTGCCGTGGGTGGGCTGAACGT
GTTGGTCGATCCGCGGGTGCTGGACAAAGCGCCGGTGATTCCACCAGGAATCTTTCGATGA

Upstream 100 bases:

>100_bases
TGACGTAACCGCCGCAGGCGGGCACCGGAAAACGTCCTCGAATACGTCCTAGCCACCGGTAAGACATTACTCCGCGTCAT
GTTCGCAAAATTTGCAAAAT

Downstream 100 bases:

>100_bases
CCGGGCCGCTCGCGGCGGCCAGGTCCGTCGCTGCCACGAAATCGATGACCGCGCCCACCGTTGATGAGCGGCCCGACATC
AAAAAGGGCCTCGCCGGCGT

Product: MmgE/PrpD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MPDQDTKVRFFRVFCWCPVLRMVRIMLMHAVRAWRSADDFPCTEHMAYKIAQVAADPVDVDPEVADMVCNRIIDNAAVSA
ASMVRRPVTVARHQALAHPVRHGAKVFGVEGSYSADWAAWANGVAARELDFHDTFLAADYSHPADNIPPLVAVAQQLGVC
GAELIRGLVTAYEIHIDLTRGICLHEHKIDHVAHLGPAVAAGIGTMLRLDQETIYHAIGQALHLTTSTRQSRKGAISSWK
AFAPAHAGKVGIEAVDRAMRGEGSPAPIWEGEDGVIAWLLAGPEHTYRVPLPAPGEPKRAILDSYTKQHSAEYQSQAPID
LACRLRERIGDLDQIASIVLHTSHHTHVVIGTGSGDPQKFDPDASRETLDHSLPYIFAVALQDGCWHHERSYAPERARRS
DTVALWHKISTVEDPEWTRRYHCADPAKKAFGARAEVTLHSGEVIVDELAVADAHPLGTRPFERKQYVEKFTELADGVVE
PVEQQRFLAVVESLADLESGAVGGLNVLVDPRVLDKAPVIPPGIFR

Sequences:

>Translated_526_residues
MPDQDTKVRFFRVFCWCPVLRMVRIMLMHAVRAWRSADDFPCTEHMAYKIAQVAADPVDVDPEVADMVCNRIIDNAAVSA
ASMVRRPVTVARHQALAHPVRHGAKVFGVEGSYSADWAAWANGVAARELDFHDTFLAADYSHPADNIPPLVAVAQQLGVC
GAELIRGLVTAYEIHIDLTRGICLHEHKIDHVAHLGPAVAAGIGTMLRLDQETIYHAIGQALHLTTSTRQSRKGAISSWK
AFAPAHAGKVGIEAVDRAMRGEGSPAPIWEGEDGVIAWLLAGPEHTYRVPLPAPGEPKRAILDSYTKQHSAEYQSQAPID
LACRLRERIGDLDQIASIVLHTSHHTHVVIGTGSGDPQKFDPDASRETLDHSLPYIFAVALQDGCWHHERSYAPERARRS
DTVALWHKISTVEDPEWTRRYHCADPAKKAFGARAEVTLHSGEVIVDELAVADAHPLGTRPFERKQYVEKFTELADGVVE
PVEQQRFLAVVESLADLESGAVGGLNVLVDPRVLDKAPVIPPGIFR
>Mature_525_residues
PDQDTKVRFFRVFCWCPVLRMVRIMLMHAVRAWRSADDFPCTEHMAYKIAQVAADPVDVDPEVADMVCNRIIDNAAVSAA
SMVRRPVTVARHQALAHPVRHGAKVFGVEGSYSADWAAWANGVAARELDFHDTFLAADYSHPADNIPPLVAVAQQLGVCG
AELIRGLVTAYEIHIDLTRGICLHEHKIDHVAHLGPAVAAGIGTMLRLDQETIYHAIGQALHLTTSTRQSRKGAISSWKA
FAPAHAGKVGIEAVDRAMRGEGSPAPIWEGEDGVIAWLLAGPEHTYRVPLPAPGEPKRAILDSYTKQHSAEYQSQAPIDL
ACRLRERIGDLDQIASIVLHTSHHTHVVIGTGSGDPQKFDPDASRETLDHSLPYIFAVALQDGCWHHERSYAPERARRSD
TVALWHKISTVEDPEWTRRYHCADPAKKAFGARAEVTLHSGEVIVDELAVADAHPLGTRPFERKQYVEKFTELADGVVEP
VEQQRFLAVVESLADLESGAVGGLNVLVDPRVLDKAPVIPPGIFR

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=432, Percent_Identity=25.2314814814815, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325258, Length=394, Percent_Identity=25.8883248730964, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 57783; Mature: 57652

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDQDTKVRFFRVFCWCPVLRMVRIMLMHAVRAWRSADDFPCTEHMAYKIAQVAADPVDV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
DPEVADMVCNRIIDNAAVSAASMVRRPVTVARHQALAHPVRHGAKVFGVEGSYSADWAAW
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHH
ANGVAARELDFHDTFLAADYSHPADNIPPLVAVAQQLGVCGAELIRGLVTAYEIHIDLTR
HCCCEEEECCCCHHEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEC
GICLHEHKIDHVAHLGPAVAAGIGTMLRLDQETIYHAIGQALHLTTSTRQSRKGAISSWK
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCHHHHH
AFAPAHAGKVGIEAVDRAMRGEGSPAPIWEGEDGVIAWLLAGPEHTYRVPLPAPGEPKRA
HHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCHHH
ILDSYTKQHSAEYQSQAPIDLACRLRERIGDLDQIASIVLHTSHHTHVVIGTGSGDPQKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
DPDASRETLDHSLPYIFAVALQDGCWHHERSYAPERARRSDTVALWHKISTVEDPEWTRR
CCCCHHHHHHHCCCCEEEEEHHCCCCCCHHCCCHHHHHCCCHHHHHHHHCCCCCCHHHHC
YHCADPAKKAFGARAEVTLHSGEVIVDELAVADAHPLGTRPFERKQYVEKFTELADGVVE
CCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PVEQQRFLAVVESLADLESGAVGGLNVLVDPRVLDKAPVIPPGIFR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PDQDTKVRFFRVFCWCPVLRMVRIMLMHAVRAWRSADDFPCTEHMAYKIAQVAADPVDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
DPEVADMVCNRIIDNAAVSAASMVRRPVTVARHQALAHPVRHGAKVFGVEGSYSADWAAW
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHH
ANGVAARELDFHDTFLAADYSHPADNIPPLVAVAQQLGVCGAELIRGLVTAYEIHIDLTR
HCCCEEEECCCCHHEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEC
GICLHEHKIDHVAHLGPAVAAGIGTMLRLDQETIYHAIGQALHLTTSTRQSRKGAISSWK
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHCCHHHHH
AFAPAHAGKVGIEAVDRAMRGEGSPAPIWEGEDGVIAWLLAGPEHTYRVPLPAPGEPKRA
HHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCHHH
ILDSYTKQHSAEYQSQAPIDLACRLRERIGDLDQIASIVLHTSHHTHVVIGTGSGDPQKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
DPDASRETLDHSLPYIFAVALQDGCWHHERSYAPERARRSDTVALWHKISTVEDPEWTRR
CCCCHHHHHHHCCCCEEEEEHHCCCCCCHHCCCHHHHHCCCHHHHHHHHCCCCCCHHHHC
YHCADPAKKAFGARAEVTLHSGEVIVDELAVADAHPLGTRPFERKQYVEKFTELADGVVE
CCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PVEQQRFLAVVESLADLESGAVGGLNVLVDPRVLDKAPVIPPGIFR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]