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The map label for this gene is purN [H]

Identifier: 148660735

GI number: 148660735

Start: 1068869

End: 1069516

Strand: Direct

Name: purN [H]

Synonym: MRA_0963

Alternate gene names: 148660735

Gene position: 1068869-1069516 (Clockwise)

Preceding gene: 148660734

Following gene: 148660736

Centisome position: 24.18

GC content: 65.12

Gene sequence:

>648_bases
GTGCAGGAACCGCTTCGTGTACCCCCGAGTGCACCTGCGCGGCTGGTAGTACTCGCGTCTGGCACCGGTTCGTTGCTGAG
ATCTCTACTCGATGCCGCTGTCGGCGACTACCCGGCACGGGTAGTCGCCGTTGGTGTGGATCGCGAATGCCGGGCCGCCG
AAATCGCCGCGGAAGCATCGGTGCCGGTGTTCACCGTTCGGCTCGCCGACCACCCCAGTCGCGATGCCTGGGACGTCGCC
ATCACCGCCGCCACCGCAGCCCATGAGCCCGACCTCGTCGTTTCTGCGGGCTTTATGAGAATCCTTGGACCGCAGTTCCT
TTCACGATTCTACGGGCGCACCCTCAACACCCACCCGGCGCTGCTGCCGGCCTTCCCCGGCACGCACGGTGTCGCTGACG
CGCTGGCCTACGGGGTGAAGGTCACCGGCGCTACGGTGCACCTGGTAGACGCTGGCACGGACACCGGGCCAATACTGGCG
CAGCAACCTGTGCCGGTGCTCGACGGTGACGACGAAGAGACTTTGCATGAACGAATCAAGGTCACCGAACGACGGCTGTT
GGTAGCGGCGGTGGCCGCACTGGCCACCCATGGCGTGACGGTGGTCGGACGAACAGCGACGATGGGACGAAAGGTAACCA
TAGGATGA

Upstream 100 bases:

>100_bases
GCCGCAACTGTCTGAGGCCTTTACTCACGGTACTGAGTCTGCACTGGGATGCAGGCTGGTGGTGCTCACACGCTTTGAGG
AGCCAGACTAGGCTCGCCGT

Downstream 100 bases:

>100_bases
GCACCGACGACGGAAGACGGCCGATCCGCCGTGCGCTGATCAGCGTGTACGACAAGACCGGGCTGGTAGACCTGGCACAG
GGCCTGAGCGCGGCCGGCGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MQEPLRVPPSAPARLVVLASGTGSLLRSLLDAAVGDYPARVVAVGVDRECRAAEIAAEASVPVFTVRLADHPSRDAWDVA
ITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILA
QQPVPVLDGDDEETLHERIKVTERRLLVAAVAALATHGVTVVGRTATMGRKVTIG

Sequences:

>Translated_215_residues
MQEPLRVPPSAPARLVVLASGTGSLLRSLLDAAVGDYPARVVAVGVDRECRAAEIAAEASVPVFTVRLADHPSRDAWDVA
ITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILA
QQPVPVLDGDDEETLHERIKVTERRLLVAAVAALATHGVTVVGRTATMGRKVTIG
>Mature_215_residues
MQEPLRVPPSAPARLVVLASGTGSLLRSLLDAAVGDYPARVVAVGVDRECRAAEIAAEASVPVFTVRLADHPSRDAWDVA
ITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILA
QQPVPVLDGDDEETLHERIKVTERRLLVAAVAALATHGVTVVGRTATMGRKVTIG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=186, Percent_Identity=37.0967741935484, Blast_Score=117, Evalue=6e-27,
Organism=Homo sapiens, GI209869995, Length=186, Percent_Identity=37.0967741935484, Blast_Score=117, Evalue=6e-27,
Organism=Homo sapiens, GI209869993, Length=186, Percent_Identity=37.0967741935484, Blast_Score=117, Evalue=6e-27,
Organism=Escherichia coli, GI1788846, Length=184, Percent_Identity=36.4130434782609, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=37.8378378378378, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=39.0374331550802, Blast_Score=126, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22416; Mature: 22416

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPLRVPPSAPARLVVLASGTGSLLRSLLDAAVGDYPARVVAVGVDRECRAAEIAAEAS
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCC
VPVFTVRLADHPSRDAWDVAITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPA
CCEEEEEECCCCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHCHHHHHHHHCCCCCCCCC
LLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILAQQPVPVLDGDDEETLHERIK
EEECCCCCCHHHHHHHHCEEECCCEEEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHH
VTERRLLVAAVAALATHGVTVVGRTATMGRKVTIG
HHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECC
>Mature Secondary Structure
MQEPLRVPPSAPARLVVLASGTGSLLRSLLDAAVGDYPARVVAVGVDRECRAAEIAAEAS
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCC
VPVFTVRLADHPSRDAWDVAITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPA
CCEEEEEECCCCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHCHHHHHHHHCCCCCCCCC
LLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILAQQPVPVLDGDDEETLHERIK
EEECCCCCCHHHHHHHHCEEECCCEEEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHH
VTERRLLVAAVAALATHGVTVVGRTATMGRKVTIG
HHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]