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The map label for this gene is pstS2

Identifier: 148660711

GI number: 148660711

Start: 1041244

End: 1042356

Strand: Reverse

Name: pstS2

Synonym: MRA_0940

Alternate gene names: 148660711

Gene position: 1042356-1041244 (Counterclockwise)

Preceding gene: 148660716

Following gene: 148660710

Centisome position: 23.58

GC content: 61.37

Gene sequence:

>1113_bases
GTGAAGTTCGCCCGATCCGGCGCAGCGGTAAGCCTGTTGGCCGCCGGCACACTGGTGTTGACAGCATGCGGCGGTGGCAC
CAACAGCTCGTCGTCAGGCGCAGGCGGCACGTCTGGGTCGGTGCACTGCGGCGGCAAGAAGGAGCTCCACTCCAGCGGCT
CGACCGCACAAGAAAATGCCATGGAGCAGTTCGTCTATGCCTACGTGCGATCGTGCCCGGGCTACACGTTGGACTACAAC
GCCAACGGGTCCGGTGCCGGGGTGACCCAGTTTCTCAACAACGAAACCGATTTCGCCGGCTCGGATGTCCCGTTGAATCC
GTCGACCGGTCAACCTGACCGGTCGGCGGAGCGGTGCGGTTCCCCGGCATGGGACCTGCCGACGGTGTTCGGCCCGATCG
CGATCACCTACAATATCAAGGGCGTGAGCACGCTGAATCTTGACGGACCCACTACCGCCAAGATTTTCAACGGCACCATC
ACCGTGTGGAATGATCCACAGATCCAAGCCCTCAACTCCGGCACCGACCTGCCGCCAACACCGATTAGCGTTATCTTCCG
CAGCGACAAGTCCGGTACGTCGGACAACTTCCAGAAATACCTCGACGGCGCATCCAACGGGGCGTGGGGCAAAGGCGCCA
GCGAAACGTTCAACGGGGGCGTCGGCGTCGGCGCCAGCGGGAACAACGGCACGTCGGCCCTACTGCAGACGACCGACGGG
TCGATCACCTACAACGAGTGGTCGTTTGCGGTGGGTAAGCAGTTGAACATGGCCCAGATCATCACGTCGGCGGGTCCGGA
TCCAGTGGCGATCACCACCGAGTCGGTCGGCAAGACAATCGCCGGGGCCAAGATCATGGGACAAGGCAACGACCTGGTAT
TGGACACGTCGTCGTTCTACAGGCCCACCCAGCCTGGCTCTTACCCGATCGTGCTGGCGACCTATGAGATCGTCTGCTCG
AAATACCCGGATGCGACGACCGGTACTGCGGTAAGGGCGTTTATGCAAGCCGCGATTGGTCCAGGCCAAGAAGGCCTGGA
CCAATACGGCTCCATTCCGTTGCCCAAATCGTTCCAAGCAAAATTGGCGGCCGCGGTGAATGCTATTTCTTGA

Upstream 100 bases:

>100_bases
TGCACAACGGTGACGGCTCGAATACCGACCCCGGGTCGGTGGCTCGGCGACCACCGGTGGCGAGTGGATCCGCCACCACG
ACCCCCTCGAGAGGAGTTGC

Downstream 100 bases:

>100_bases
CCTAGTGAAGGGAATTCGACGGTGAGCGATGCCGTTCCGCAGGTAGGGTCGCAATTTGGGCCGTATCAGCTATTGCGGCT
GCTGGGCCGAGGCGGGATGG

Product: phosphate ABC transporter substrate-binding protein PstS2

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 2; PstS-2

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKFARSGAAVSLLAAGTLVLTACGGGTNSSSSGAGGTSGSVHCGGKKELHSSGSTAQENAMEQFVYAYVRSCPGYTLDYN
ANGSGAGVTQFLNNETDFAGSDVPLNPSTGQPDRSAERCGSPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTI
TVWNDPQIQALNSGTDLPPTPISVIFRSDKSGTSDNFQKYLDGASNGAWGKGASETFNGGVGVGASGNNGTSALLQTTDG
SITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIAGAKIMGQGNDLVLDTSSFYRPTQPGSYPIVLATYEIVCS
KYPDATTGTAVRAFMQAAIGPGQEGLDQYGSIPLPKSFQAKLAAAVNAIS

Sequences:

>Translated_370_residues
MKFARSGAAVSLLAAGTLVLTACGGGTNSSSSGAGGTSGSVHCGGKKELHSSGSTAQENAMEQFVYAYVRSCPGYTLDYN
ANGSGAGVTQFLNNETDFAGSDVPLNPSTGQPDRSAERCGSPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTI
TVWNDPQIQALNSGTDLPPTPISVIFRSDKSGTSDNFQKYLDGASNGAWGKGASETFNGGVGVGASGNNGTSALLQTTDG
SITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIAGAKIMGQGNDLVLDTSSFYRPTQPGSYPIVLATYEIVCS
KYPDATTGTAVRAFMQAAIGPGQEGLDQYGSIPLPKSFQAKLAAAVNAIS
>Mature_370_residues
MKFARSGAAVSLLAAGTLVLTACGGGTNSSSSGAGGTSGSVHCGGKKELHSSGSTAQENAMEQFVYAYVRSCPGYTLDYN
ANGSGAGVTQFLNNETDFAGSDVPLNPSTGQPDRSAERCGSPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTI
TVWNDPQIQALNSGTDLPPTPISVIFRSDKSGTSDNFQKYLDGASNGAWGKGASETFNGGVGVGASGNNGTSALLQTTDG
SITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIAGAKIMGQGNDLVLDTSSFYRPTQPGSYPIVLATYEIVCS
KYPDATTGTAVRAFMQAAIGPGQEGLDQYGSIPLPKSFQAKLAAAVNAIS

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family

Homologues:

Organism=Escherichia coli, GI2367271, Length=301, Percent_Identity=31.5614617940199, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PSTS2_MYCTU (O05870)

Other databases:

- EMBL:   Z48056
- EMBL:   BX842575
- EMBL:   AE000516
- PIR:   D70584
- RefSeq:   NP_335392.1
- RefSeq:   YP_177769.1
- ProteinModelPortal:   O05870
- SMR:   O05870
- EnsemblBacteria:   EBMYCT00000000571
- EnsemblBacteria:   EBMYCT00000072155
- GeneID:   885613
- GeneID:   926275
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0959
- KEGG:   mtu:Rv0932c
- TIGR:   MT0959
- TubercuList:   Rv0932c
- GeneTree:   EBGT00050000017160
- HOGENOM:   HBG428828
- OMA:   MERKILY
- ProtClustDB:   CLSK799391
- InterPro:   IPR005673
- InterPro:   IPR006059
- TIGRFAMs:   TIGR00975

Pfam domain/function: PF01547 SBP_bac_1

EC number: NA

Molecular weight: Translated: 37864; Mature: 37864

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFARSGAAVSLLAAGTLVLTACGGGTNSSSSGAGGTSGSVHCGGKKELHSSGSTAQENA
CCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHCCCCHHHHHH
MEQFVYAYVRSCPGYTLDYNANGSGAGVTQFLNNETDFAGSDVPLNPSTGQPDRSAERCG
HHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCC
SPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTITVWNDPQIQALNSGTDLPPT
CCCCCCCHHHCCEEEEEEECCEEEEECCCCCEEEEEECEEEEECCCCEEEECCCCCCCCC
PISVIFRSDKSGTSDNFQKYLDGASNGAWGKGASETFNGGVGVGASGNNGTSALLQTTDG
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCC
SITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIAGAKIMGQGNDLVLDTSSFY
CEEECCCCCHHCCCCCHHHHHHCCCCCCEEEEHHHHCCHHCCEEEEECCCEEEEECHHCC
RPTQPGSYPIVLATYEIVCSKYPDATTGTAVRAFMQAAIGPGQEGLDQYGSIPLPKSFQA
CCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHH
KLAAAVNAIS
HHHHHHHHCC
>Mature Secondary Structure
MKFARSGAAVSLLAAGTLVLTACGGGTNSSSSGAGGTSGSVHCGGKKELHSSGSTAQENA
CCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHCCCCHHHHHH
MEQFVYAYVRSCPGYTLDYNANGSGAGVTQFLNNETDFAGSDVPLNPSTGQPDRSAERCG
HHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCC
SPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTITVWNDPQIQALNSGTDLPPT
CCCCCCCHHHCCEEEEEEECCEEEEECCCCCEEEEEECEEEEECCCCEEEECCCCCCCCC
PISVIFRSDKSGTSDNFQKYLDGASNGAWGKGASETFNGGVGVGASGNNGTSALLQTTDG
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCC
SITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIAGAKIMGQGNDLVLDTSSFY
CEEECCCCCHHCCCCCHHHHHHCCCCCCEEEEHHHHCCHHCCEEEEECCCEEEEECHHCC
RPTQPGSYPIVLATYEIVCSKYPDATTGTAVRAFMQAAIGPGQEGLDQYGSIPLPKSFQA
CCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHH
KLAAAVNAIS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9139906; 9634230; 12218036