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The map label for this gene is gltA

Identifier: 148660674

GI number: 148660674

Start: 1000779

End: 1002074

Strand: Direct

Name: gltA

Synonym: MRA_0904

Alternate gene names: 148660674

Gene position: 1000779-1002074 (Clockwise)

Preceding gene: 148660673

Following gene: 148660677

Centisome position: 22.64

GC content: 62.42

Gene sequence:

>1296_bases
GTGGCCGACACCGACGACACCGCAACCCTCCGTTACCCGGGAGGCGAGATCGACCTGCAGATCGTGCACGCCACCGAAGG
CGCCGACGGCATTGCGCTCGGGCCGCTGCTGGCAAAAACCGGGCACACCACGTTCGACGTCGGCTTCGCCAACACGGCCG
CCGCTAAAAGCTCCATCACCTACATCGACGGAGATGCCGGCATTCTGCGTTATCGCGGCTACCCGATCGACCAACTGGCG
GAGAAGTCAACCTTCATCGAGGTCTGCTACCTGTTGATTTACGGCGAGCTGCCCGATACCGACCAGCTTGCCCAGTTCAC
CGGCCGGATCCAGCGCCACACCATGCTGCACGAGGATCTCAAGCGGTTCTTCGACGGCTTTCCGCGCAATGCCCACCCGA
TGCCGGTGTTGTCCAGCGTGGTCAATGCGCTGTCGGCGTACTACCAGGATGCTCTGGACCCCATGGACAACGGTCAAGTC
GAGCTGTCGACCATTCGGCTGCTGGCCAAGCTGCCCACCATCGCCGCGTACGCCTACAAGAAATCGGTCGGCCAGCCCTT
CCTCTACCCAGATAACTCACTGACGCTGGTGGAGAACTTCCTACGGTTGACGTTCGGATTTCCCGCCGAGCCCTACCAGG
CCGACCCCGAGGTGGTGCGGGCGCTGGACATGTTGTTCATCTTGCACGCCGACCACGAGCAGAACTGCTCGACGTCGACG
GTTCGGCTGGTTGGCTCGTCGCGAGCCAACCTGTTCACCTCGATCTCGGGTGGCATCAACGCACTATGGGGTCCGCTTCA
TGGCGGCGCCAATCAGGCTGTCCTGGAGATGCTCGAGGGCATTCGCGACAGCGGCGACGACGTCAGCGAGTTTGTACGCA
AGGTCAAGAACCGCGAGGCCGGGGTCAAATTGATGGGTTTCGGTCATCGTGTCTACAAGAACTACGATCCGCGGGCCCGC
ATCGTCAAGGAACAGGCCGACAAGATCCTGGCCAAGCTCGGCGGCGATGACTCCTTGCTGGGCATCGCCAAGGAGCTCGA
AGAGGCGGCGCTGACCGACGACTACTTCATCGAACGCAAGCTTTACCCCAACGTCGACTTCTACACCGGCCTGATCTACC
GGGCCCTCGGCTTCCCGACCAGGATGTTCACCGTGTTGTTTGCCCTGGGCAGGCTTCCCGGCTGGATCGCGCACTGGCGT
GAGATGCACGACGAGGGCGACAGCAAGATCGGCCGGCCCCGCCAGATCTACACCGGCTACACGGAGCGCGACTACGTCAC
CATAGACGCGCGGTAG

Upstream 100 bases:

>100_bases
AGACCCCACCCCGGGCAGGCGGGTCATCCCGATAGCGACTACCTTCAGCTATAAGCACTTAGTGGGGCAGCCATATCAGC
CAAAGCGCGAAGGGGTTCTC

Downstream 100 bases:

>100_bases
GCCGGCGAGCAGACGCAAAAGCCCCCTAAACCGGCAGGTATTAGGGGCTTTTGCGTCTGCTCGCCAGGCAAGCCAGCACT
GCCATCGCGGCGTTGTGACC

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MADTDDTATLRYPGGEIDLQIVHATEGADGIALGPLLAKTGHTTFDVGFANTAAAKSSITYIDGDAGILRYRGYPIDQLA
EKSTFIEVCYLLIYGELPDTDQLAQFTGRIQRHTMLHEDLKRFFDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQV
ELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNSLTLVENFLRLTFGFPAEPYQADPEVVRALDMLFILHADHEQNCSTST
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRDSGDDVSEFVRKVKNREAGVKLMGFGHRVYKNYDPRAR
IVKEQADKILAKLGGDDSLLGIAKELEEAALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWR
EMHDEGDSKIGRPRQIYTGYTERDYVTIDAR

Sequences:

>Translated_431_residues
MADTDDTATLRYPGGEIDLQIVHATEGADGIALGPLLAKTGHTTFDVGFANTAAAKSSITYIDGDAGILRYRGYPIDQLA
EKSTFIEVCYLLIYGELPDTDQLAQFTGRIQRHTMLHEDLKRFFDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQV
ELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNSLTLVENFLRLTFGFPAEPYQADPEVVRALDMLFILHADHEQNCSTST
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRDSGDDVSEFVRKVKNREAGVKLMGFGHRVYKNYDPRAR
IVKEQADKILAKLGGDDSLLGIAKELEEAALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWR
EMHDEGDSKIGRPRQIYTGYTERDYVTIDAR
>Mature_430_residues
ADTDDTATLRYPGGEIDLQIVHATEGADGIALGPLLAKTGHTTFDVGFANTAAAKSSITYIDGDAGILRYRGYPIDQLAE
KSTFIEVCYLLIYGELPDTDQLAQFTGRIQRHTMLHEDLKRFFDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQVE
LSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNSLTLVENFLRLTFGFPAEPYQADPEVVRALDMLFILHADHEQNCSTSTV
RLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRDSGDDVSEFVRKVKNREAGVKLMGFGHRVYKNYDPRARI
VKEQADKILAKLGGDDSLLGIAKELEEAALTDDYFIERKLYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWRE
MHDEGDSKIGRPRQIYTGYTERDYVTIDAR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family

Homologues:

Organism=Homo sapiens, GI38327625, Length=392, Percent_Identity=28.0612244897959, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=52.9411764705882, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1786527, Length=387, Percent_Identity=31.5245478036176, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17555174, Length=380, Percent_Identity=27.8947368421053, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324328, Length=366, Percent_Identity=28.4153005464481, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319850, Length=328, Percent_Identity=26.5243902439024, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6325257, Length=377, Percent_Identity=25.9946949602122, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24640126, Length=388, Percent_Identity=28.8659793814433, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24640124, Length=388, Percent_Identity=28.8659793814433, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356863, Length=370, Percent_Identity=27.5675675675676, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CISY1_MYCTU (Q10530)

Other databases:

- EMBL:   BX842574
- EMBL:   AE000516
- PIR:   E70782
- RefSeq:   NP_215411.1
- RefSeq:   NP_335352.1
- ProteinModelPortal:   Q10530
- SMR:   Q10530
- EnsemblBacteria:   EBMYCT00000002706
- EnsemblBacteria:   EBMYCT00000070428
- GeneID:   885208
- GeneID:   926234
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0920
- KEGG:   mtu:Rv0896
- TIGR:   MT0920
- TubercuList:   Rv0896
- GeneTree:   EBGT00050000016202
- HOGENOM:   HBG649489
- OMA:   WCAQLLE
- ProtClustDB:   PRK05614
- BioCyc:   MetaCyc:MONOMER-11944
- BRENDA:   2.3.3.1
- GO:   GO:0005829
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953
- Gene3D:   G3DSA:1.10.580.10
- Gene3D:   G3DSA:1.10.230.10
- PANTHER:   PTHR11739
- PRINTS:   PR00143
- TIGRFAMs:   TIGR01798

Pfam domain/function: PF00285 Citrate_synt; SSF48256 Citrate_synthase_core

EC number: =2.3.3.1

Molecular weight: Translated: 47979; Mature: 47848

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: ACT_SITE 309-309 ACT_SITE 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTDDTATLRYPGGEIDLQIVHATEGADGIALGPLLAKTGHTTFDVGFANTAAAKSSIT
CCCCCCCEEEEECCCCEEEEEEEECCCCCCCEECHHHHHCCCCEEEECCCCHHHCCCCEE
YIDGDAGILRYRGYPIDQLAEKSTFIEVCYLLIYGELPDTDQLAQFTGRIQRHTMLHEDL
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KRFFDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQVELSTIRLLAKLPTIAAYAYK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
KSVGQPFLYPDNSLTLVENFLRLTFGFPAEPYQADPEVVRALDMLFILHADHEQNCSTST
HHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCE
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRDSGDDVSEFVRKVKNREA
EEEECCCCCHHEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
GVKLMGFGHRVYKNYDPRARIVKEQADKILAKLGGDDSLLGIAKELEEAALTDDYFIERK
CEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHC
LYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWREMHDEGDSKIGRPRQIYTGY
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECC
TERDYVTIDAR
CCCCEEEEECC
>Mature Secondary Structure 
ADTDDTATLRYPGGEIDLQIVHATEGADGIALGPLLAKTGHTTFDVGFANTAAAKSSIT
CCCCCCEEEEECCCCEEEEEEEECCCCCCCEECHHHHHCCCCEEEECCCCHHHCCCCEE
YIDGDAGILRYRGYPIDQLAEKSTFIEVCYLLIYGELPDTDQLAQFTGRIQRHTMLHEDL
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KRFFDGFPRNAHPMPVLSSVVNALSAYYQDALDPMDNGQVELSTIRLLAKLPTIAAYAYK
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
KSVGQPFLYPDNSLTLVENFLRLTFGFPAEPYQADPEVVRALDMLFILHADHEQNCSTST
HHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCE
VRLVGSSRANLFTSISGGINALWGPLHGGANQAVLEMLEGIRDSGDDVSEFVRKVKNREA
EEEECCCCCHHEEECCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
GVKLMGFGHRVYKNYDPRARIVKEQADKILAKLGGDDSLLGIAKELEEAALTDDYFIERK
CEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHC
LYPNVDFYTGLIYRALGFPTRMFTVLFALGRLPGWIAHWREMHDEGDSKIGRPRQIYTGY
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEECC
TERDYVTIDAR
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036