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The map label for this gene is fadA

Identifier: 148660636

GI number: 148660636

Start: 956383

End: 957594

Strand: Direct

Name: fadA

Synonym: MRA_0867

Alternate gene names: 148660636

Gene position: 956383-957594 (Clockwise)

Preceding gene: 148660634

Following gene: 148660637

Centisome position: 21.64

GC content: 67.08

Gene sequence:

>1212_bases
ATGTCCGAAGAAGCCTTCATCTACGAGGCCATCCGCACCCCGCGCGGCAAACAAAAGAACGGATCGTTGCACGAAGTCAA
GCCATTGAGCCTGGTCGTCGGCCTGATCGACGAGCTGCGCAAGCGCCATCCCGACCTCGACGAGAACCTGATCAGCGACG
TCATCTTGGGCTGCGTCTCACCGGTGGGCGACCAGGGCGGCGACATCGCCCGCGCCGCAGTGCTGGCATCGGGCATGCCG
GTCACCTCCGGCGGTGTGCAGCTCAACCGGTTCTGCGCGTCCGGCCTGGAGGCCGTCAACACCGCCGCGCAGAAGGTGCG
TTCGGGCTGGGATGACCTGGTGCTGGCCGGCGGCGTGGAGTCGATGAGCCGGGTGCCGATGGGCTCCGACGGCGGCGCTA
TGGGCCTGGACCCGGCGACCAACTACGACGTCATGTTCGTCCCGCAGAGCATCGGCGCCGACCTGATCGCCACCATCGAG
GGCTTCTCCCGCGAAGACGTCGACGCCTACGCGCTACGCAGCCAGCAAAAGGCCGCCGAGGCGTGGTCGGGCGGCTACTT
CGCCAAGTCGGTGGTGCCGGTGCGCGACCAGAACGGCCTGCTGATCCTCGATCATGACGAACACATGCGGCCGGACACCA
CCAAGGAGGGTCTGGCCAAGCTGAAGCCGGCCTTCGAAGGCCTGGCCGCGCTGGGCGGTTTCGACGACGTGGCGCTGCAG
AAGTACCACTGGGTGGAAAAGATCAACCACGTACACACCGGCGGCAACAGCTCGGGGATCGTCGACGGTGCCGCGCTGGT
GATGATCGGTTCCGCGGCCGCCGGCAAGTTGCAGGGCCTGACTCCGCGGGCGCGCATCGTCGCCACCGCCACCAGCGGCG
CCGACCCGGTGATCATGCTCACCGGCCCCACCCCGGCCACCCGCAAGGTGCTCGACCGCGCCGGGCTGACCGTCGACGAC
ATCGACCTGTTCGAGCTCAACGAGGCGTTCGCGTCGGTGGTGCTGAAGTTCCAGAAGGACCTCAACATTCCCGACGAGAA
GCTCAACGTCAACGGTGGCGCCATCGCGATGGGCCACCCGCTGGGTGCCACCGGCGCGATGATCCTGGGCACCATGGTCG
ACGAACTGGAGCGCCGCAACGCCCGACGTGCACTCATCACGCTGTGCATCGGGGGCGGCATGGGTGTCGCGACGATCATC
GAGAGGGTTTAA

Upstream 100 bases:

>100_bases
ACAGGTTGGCCGGGGGCCCTGTTAGGGTGCCGGTACGGGACCTAGTCTTGAAGAAGGATCCAAACCCCCTTTTGTGGAAT
TTGTGGAACAGGAAATCGAC

Downstream 100 bases:

>100_bases
CAGCATGCCAGACAACACAATCCAGTGGGACAAGGATGCCGACGGCATCGTCACGCTGACCATGGACGATCCCTCCGGGT
CAACCAACGTGATGAACGAG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEEAFIYEAIRTPRGKQKNGSLHEVKPLSLVVGLIDELRKRHPDLDENLISDVILGCVSPVGDQGGDIARAAVLASGMP
VTSGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQSIGADLIATIE
GFSREDVDAYALRSQQKAAEAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTTKEGLAKLKPAFEGLAALGGFDDVALQ
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSAAAGKLQGLTPRARIVATATSGADPVIMLTGPTPATRKVLDRAGLTVDD
IDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERRNARRALITLCIGGGMGVATII
ERV

Sequences:

>Translated_403_residues
MSEEAFIYEAIRTPRGKQKNGSLHEVKPLSLVVGLIDELRKRHPDLDENLISDVILGCVSPVGDQGGDIARAAVLASGMP
VTSGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQSIGADLIATIE
GFSREDVDAYALRSQQKAAEAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTTKEGLAKLKPAFEGLAALGGFDDVALQ
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSAAAGKLQGLTPRARIVATATSGADPVIMLTGPTPATRKVLDRAGLTVDD
IDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERRNARRALITLCIGGGMGVATII
ERV
>Mature_402_residues
SEEAFIYEAIRTPRGKQKNGSLHEVKPLSLVVGLIDELRKRHPDLDENLISDVILGCVSPVGDQGGDIARAAVLASGMPV
TSGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVESMSRVPMGSDGGAMGLDPATNYDVMFVPQSIGADLIATIEG
FSREDVDAYALRSQQKAAEAWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTTKEGLAKLKPAFEGLAALGGFDDVALQK
YHWVEKINHVHTGGNSSGIVDGAALVMIGSAAAGKLQGLTPRARIVATATSGADPVIMLTGPTPATRKVLDRAGLTVDDI
DLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHPLGATGAMILGTMVDELERRNARRALITLCIGGGMGVATIIE
RV

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI167614485, Length=365, Percent_Identity=37.2602739726027, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI148539872, Length=416, Percent_Identity=36.2980769230769, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=37, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4557237, Length=419, Percent_Identity=31.0262529832936, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4504327, Length=383, Percent_Identity=29.7650130548303, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=51.685393258427, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1787663, Length=423, Percent_Identity=43.7352245862884, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI48994986, Length=409, Percent_Identity=38.8753056234719, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI87082165, Length=417, Percent_Identity=40.0479616306954, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1788554, Length=416, Percent_Identity=38.2211538461538, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788683, Length=442, Percent_Identity=28.9592760180996, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI133906874, Length=381, Percent_Identity=38.8451443569554, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17535921, Length=414, Percent_Identity=32.8502415458937, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=418, Percent_Identity=30.8612440191388, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17551802, Length=417, Percent_Identity=28.0575539568345, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=26.9135802469136, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=36.2068965517241, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6325229, Length=424, Percent_Identity=30.8962264150943, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24655093, Length=415, Percent_Identity=37.8313253012048, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI17648125, Length=349, Percent_Identity=36.1031518624642, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24640423, Length=367, Percent_Identity=30.5177111716621, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137578, Length=414, Percent_Identity=29.951690821256, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y0859_MYCTU (O53871)

Other databases:

- EMBL:   BX842574
- PIR:   C70815
- RefSeq:   NP_215374.1
- HSSP:   P27796
- ProteinModelPortal:   O53871
- SMR:   O53871
- EnsemblBacteria:   EBMYCT00000002324
- GeneID:   885774
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv0859
- TubercuList:   Rv0859
- GeneTree:   EBGT00070000031748
- HOGENOM:   HBG370930
- OMA:   DVEAVHH
- ProtClustDB:   PRK08242
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: 2.3.1.16

Molecular weight: Translated: 42415; Mature: 42284

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 92-92 ACT_SITE 359-359 ACT_SITE 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEAFIYEAIRTPRGKQKNGSLHEVKPLSLVVGLIDELRKRHPDLDENLISDVILGCVS
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PVGDQGGDIARAAVLASGMPVTSGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVE
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHH
SMSRVPMGSDGGAMGLDPATNYDVMFVPQSIGADLIATIEGFSREDVDAYALRSQQKAAE
HHHCCCCCCCCCCCCCCCCCCCEEEEECHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHH
AWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTTKEGLAKLKPAFEGLAALGGFDDVALQ
HHCCCCCHHEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSAAAGKLQGLTPRARIVATATSGADPVIML
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEE
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHP
ECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCEEEECCC
LGATGAMILGTMVDELERRNARRALITLCIGGGMGVATIIERV
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
SEEAFIYEAIRTPRGKQKNGSLHEVKPLSLVVGLIDELRKRHPDLDENLISDVILGCVS
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
PVGDQGGDIARAAVLASGMPVTSGGVQLNRFCASGLEAVNTAAQKVRSGWDDLVLAGGVE
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHH
SMSRVPMGSDGGAMGLDPATNYDVMFVPQSIGADLIATIEGFSREDVDAYALRSQQKAAE
HHHCCCCCCCCCCCCCCCCCCCEEEEECHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHH
AWSGGYFAKSVVPVRDQNGLLILDHDEHMRPDTTKEGLAKLKPAFEGLAALGGFDDVALQ
HHCCCCCHHEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
KYHWVEKINHVHTGGNSSGIVDGAALVMIGSAAAGKLQGLTPRARIVATATSGADPVIML
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEE
TGPTPATRKVLDRAGLTVDDIDLFELNEAFASVVLKFQKDLNIPDEKLNVNGGAIAMGHP
ECCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCEEEECCC
LGATGAMILGTMVDELERRNARRALITLCIGGGMGVATIIERV
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230