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The map label for this gene is ssuD [C]

Identifier: 148660569

GI number: 148660569

Start: 886103

End: 887146

Strand: Reverse

Name: ssuD [C]

Synonym: MRA_0801

Alternate gene names: 148660569

Gene position: 887146-886103 (Counterclockwise)

Preceding gene: 148660570

Following gene: 148660568

Centisome position: 20.07

GC content: 64.46

Gene sequence:

>1044_bases
ATGAACGCCAAAGACGACCCGCACTTCGGACTCATGTTGGCTGCGACGGTTAATGGCCTGGCGGTCGGCAGTTACCGCGA
AATGGTTGTCGTCTCGCAGACGGCGGAAGAATACGGATTCGATTCAGTGTGGTTATGCGACCACTTCCTGACGATAAGCC
CCGGCGAGTATGCGAAGGTCGCCGGCATCGCCGCCGACACCGGCTCCGCGACCGGCACCGAAACCGGCGGGGCGGGCCAG
TGCGCCCCTTCGAGGTCGCTGCCCCTGCTCGAGTGCTGGACCGCACTTGCGGCATTGTCCCGCGACACCACGAAGCTGCG
GTTGGGCACCAGCGTGCTGTGCAATTCCTACCGTCATCCTTCCGTGCTGGCCAAGATGGCTGCGACGCTGGACGTCATTT
CCCAGGGACGTCTCGATCTGGGATTGGGCGCCGGCTGGTTTCGGCGCGAGTCGCAAGCCTACGGCATCCCGTTCCCGCCG
GTTGGCGACCGAGTCTCCGCGCTTGCCGAGTCGCTGCAGGTCATCAAGGCCGTGTGGACCGAGCCAAACCCCACCTACGC
CGGCCGGTTTTACACCTTGGACGGCGCCACCTGCGATCCGCCGCCGGTGCAACGGCCACATCCTCCGTTGTGGATCGGCG
GGGAAGGCGACCGGGTCCAGCGCATCGCCGCTAAGCATGCCCAGGGCCTGAACGTGCGCTGGTGGTCGCCGCAGCAAGTC
ACCCAACGCCGCGGATTTCTCACCCAGGCGTCGGAGGCGGCCGGGCGTGACCCCGACACGCTGCGACTGTCGGTCACGCT
GCTGCTGGCACCCACCCAGTCCGGCGAGGAAGAAGTCCGGATCCGCGAAGAATTCGCGTCCATCCCCGAGCCGGGGCTCA
TCGTCGGGACACCCGACAGGTGTGTCGAGCGCATTCGTGAATACCAGGACCGCGGTGTCGGCCATTTTCTCTTCACGATT
CCACACGTCGTGAAGTCCGATTATCTGCACATCATCGGCAGTGACATCATTCCGCGGGTCAAAACTGAGGTCACGATTCC
ATGA

Upstream 100 bases:

>100_bases
TCGTGTTCCACCATCGCCCCGAGCGGTACCAGTTCTCCGTCACGTTACCCCGAACGTTGCCCGGATCAGGTGCCGGAATT
ATCGAGAAACGAGATTTCGC

Downstream 100 bases:

>100_bases
CGCTAGCCAACAATGGAACCGGCATGGACCACTTTCTGACGCCCACGGAGTACCTCGACGCGGGCCATCCGCTCGTTCGT
ACGACGGCAGCAACCCTCAT

Product: hypothetical protein

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEYGFDSVWLCDHFLTISPGEYAKVAGIAADTGSATGTETGGAGQ
CAPSRSLPLLECWTALAALSRDTTKLRLGTSVLCNSYRHPSVLAKMAATLDVISQGRLDLGLGAGWFRRESQAYGIPFPP
VGDRVSALAESLQVIKAVWTEPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGEGDRVQRIAAKHAQGLNVRWWSPQQV
TQRRGFLTQASEAAGRDPDTLRLSVTLLLAPTQSGEEEVRIREEFASIPEPGLIVGTPDRCVERIREYQDRGVGHFLFTI
PHVVKSDYLHIIGSDIIPRVKTEVTIP

Sequences:

>Translated_347_residues
MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEYGFDSVWLCDHFLTISPGEYAKVAGIAADTGSATGTETGGAGQ
CAPSRSLPLLECWTALAALSRDTTKLRLGTSVLCNSYRHPSVLAKMAATLDVISQGRLDLGLGAGWFRRESQAYGIPFPP
VGDRVSALAESLQVIKAVWTEPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGEGDRVQRIAAKHAQGLNVRWWSPQQV
TQRRGFLTQASEAAGRDPDTLRLSVTLLLAPTQSGEEEVRIREEFASIPEPGLIVGTPDRCVERIREYQDRGVGHFLFTI
PHVVKSDYLHIIGSDIIPRVKTEVTIP
>Mature_347_residues
MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEYGFDSVWLCDHFLTISPGEYAKVAGIAADTGSATGTETGGAGQ
CAPSRSLPLLECWTALAALSRDTTKLRLGTSVLCNSYRHPSVLAKMAATLDVISQGRLDLGLGAGWFRRESQAYGIPFPP
VGDRVSALAESLQVIKAVWTEPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGEGDRVQRIAAKHAQGLNVRWWSPQQV
TQRRGFLTQASEAAGRDPDTLRLSVTLLLAPTQSGEEEVRIREEFASIPEPGLIVGTPDRCVERIREYQDRGVGHFLFTI
PHVVKSDYLHIIGSDIIPRVKTEVTIP

Specific function: Involved In Desulfonation Of Aliphatic Sulfonates. Catalyzes The Conversion Of Pentanesulfonic Acid To Sulfite And Pentaldehyde And Is Able To Desulfonate A Wide Range Of Sulfonated Substrates Including C-2 To C-10 Unsubstituted Linear Alkanesulfonates, S

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.tuberculosis Rv2161c and Rv3079c [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=197, Percent_Identity=26.9035532994924, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787247, Length=182, Percent_Identity=28.021978021978, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019921
- InterPro:   IPR011251
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 37715; Mature: 37715

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEYGFDSVWLCDHFLTISPGEYAKV
CCCCCCCCCCEEEEEECCCEEECCCCEEEEEECCHHHHCCCEEEEEEEEEEECCCCCCEE
AGIAADTGSATGTETGGAGQCAPSRSLPLLECWTALAALSRDTTKLRLGTSVLCNSYRHP
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCH
SVLAKMAATLDVISQGRLDLGLGAGWFRRESQAYGIPFPPVGDRVSALAESLQVIKAVWT
HHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
EPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGEGDRVQRIAAKHAQGLNVRWWSPQQV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEECCHHHH
TQRRGFLTQASEAAGRDPDTLRLSVTLLLAPTQSGEEEVRIREEFASIPEPGLIVGTPDR
HHHHCCHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCHHH
CVERIREYQDRGVGHFLFTIPHVVKSDYLHIIGSDIIPRVKTEVTIP
HHHHHHHHHHCCCCEEEHHHHHHHHHCHHEECCCCCCCCCCEEEECC
>Mature Secondary Structure
MNAKDDPHFGLMLAATVNGLAVGSYREMVVVSQTAEEYGFDSVWLCDHFLTISPGEYAKV
CCCCCCCCCCEEEEEECCCEEECCCCEEEEEECCHHHHCCCEEEEEEEEEEECCCCCCEE
AGIAADTGSATGTETGGAGQCAPSRSLPLLECWTALAALSRDTTKLRLGTSVLCNSYRHP
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCH
SVLAKMAATLDVISQGRLDLGLGAGWFRRESQAYGIPFPPVGDRVSALAESLQVIKAVWT
HHHHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
EPNPTYAGRFYTLDGATCDPPPVQRPHPPLWIGGEGDRVQRIAAKHAQGLNVRWWSPQQV
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCEEECCHHHH
TQRRGFLTQASEAAGRDPDTLRLSVTLLLAPTQSGEEEVRIREEFASIPEPGLIVGTPDR
HHHHCCHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCHHH
CVERIREYQDRGVGHFLFTIPHVVKSDYLHIIGSDIIPRVKTEVTIP
HHHHHHHHHHCCCCEEEHHHHHHHHHCHHEECCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]