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The map label for this gene is rmlA

Identifier: 148660100

GI number: 148660100

Start: 400020

End: 400886

Strand: Direct

Name: rmlA

Synonym: MRA_0343

Alternate gene names: 148660100

Gene position: 400020-400886 (Clockwise)

Preceding gene: 148660099

Following gene: 148660102

Centisome position: 9.05

GC content: 61.36

Gene sequence:

>867_bases
ATGCGCGGGATCATCTTGGCCGGCGGTTCGGGCACCCGGCTGTACCCGATCACCATGGGGATCAGCAAGCAGCTGCTGCC
GGTCTACGACAAACCGATGATCTACTACCCGCTCACCACGCTGATGATGGCTGGGATCCGAGACATTCAGTTGATCACCA
CCCCGCATGACGCGCCCGGCTTTCATCGACTCCTGGGCGACGGCGCGCACTTGGGAGTGAACATCAGCTACGCCACCCAG
GATCAGCCTGACGGTCTGGCGCAGGCGTTCGTCATTGGCGCCAACCACATCGGCGCCGATTCGGTGGCATTGGTGTTGGG
GGACAACATCTTCTACGGCCCAGGTCTGGGGACCAGCCTGAAGCGCTTCCAATCCATCAGTGGTGGAGCAATTTTCGCCT
ATTGGGTAGCCAACCCGTCGGCCTATGGTGTCGTTGAGTTCGGCGCCGAGGGCATGGCGCTGTCTCTGGAGGAGAAGCCG
GTGACCCCGAAGTCGAATTACGCGGTGCCGGGCCTGTATTTCTATGACAACGATGTGATCGAAATCGCCAGGGGTTTAAA
GAAATCAGCGCGCGGGGAGTACGAGATCACCGAGGTCAACCAGGTCTACCTCAATCAGGGTAGGTTGGCGGTCGAGGTGC
TGGCCCGCGGGACAGCGTGGCTGGACACCGGGACATTCGACTCGCTGCTGGACGCCGCCGATTTCGTCCGGACCCTGGAG
CGTCGGCAGGGCCTGAAGGTCAGCATCCCCGAAGAAGTGGCGTGGCGCATGGGCTGGATCGACGACGAGCAGCTGGTGCA
GCGAGCCCGTGCTCTGGTCAAGTCCGGATATGGTAACTACCTGCTGGAGTTGTTGGAGCGCAACTGA

Upstream 100 bases:

>100_bases
TTTTCCGGCACGAAGACTTGGCGTCGGCGCTGGCGGCCACCGAACTCACCGAGGACGATTTGGTCGATTGAGGTCGGCGA
ACGGCAGTTAGGAGCCAGTT

Downstream 100 bases:

>100_bases
TTTCGGCGGGTTATTGTCGGTGATTATGGAACCCCCTGGTAGCCCGTCCTGGATGAGCAGCCCACCGGACCAGCCATTGC
CGAACAGCCCGCCGTTGGCG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLTTLMMAGIRDIQLITTPHDAPGFHRLLGDGAHLGVNISYATQ
DQPDGLAQAFVIGANHIGADSVALVLGDNIFYGPGLGTSLKRFQSISGGAIFAYWVANPSAYGVVEFGAEGMALSLEEKP
VTPKSNYAVPGLYFYDNDVIEIARGLKKSARGEYEITEVNQVYLNQGRLAVEVLARGTAWLDTGTFDSLLDAADFVRTLE
RRQGLKVSIPEEVAWRMGWIDDEQLVQRARALVKSGYGNYLLELLERN

Sequences:

>Translated_288_residues
MRGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLTTLMMAGIRDIQLITTPHDAPGFHRLLGDGAHLGVNISYATQ
DQPDGLAQAFVIGANHIGADSVALVLGDNIFYGPGLGTSLKRFQSISGGAIFAYWVANPSAYGVVEFGAEGMALSLEEKP
VTPKSNYAVPGLYFYDNDVIEIARGLKKSARGEYEITEVNQVYLNQGRLAVEVLARGTAWLDTGTFDSLLDAADFVRTLE
RRQGLKVSIPEEVAWRMGWIDDEQLVQRARALVKSGYGNYLLELLERN
>Mature_288_residues
MRGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLTTLMMAGIRDIQLITTPHDAPGFHRLLGDGAHLGVNISYATQ
DQPDGLAQAFVIGANHIGADSVALVLGDNIFYGPGLGTSLKRFQSISGGAIFAYWVANPSAYGVVEFGAEGMALSLEEKP
VTPKSNYAVPGLYFYDNDVIEIARGLKKSARGEYEITEVNQVYLNQGRLAVEVLARGTAWLDTGTFDSLLDAADFVRTLE
RRQGLKVSIPEEVAWRMGWIDDEQLVQRARALVKSGYGNYLLELLERN

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide,

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1788351, Length=284, Percent_Identity=61.9718309859155, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=59.4405594405594, Blast_Score=354, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI133931050, Length=184, Percent_Identity=26.6304347826087, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320148, Length=255, Percent_Identity=26.2745098039216, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLA_MYCTU (P72017)

Other databases:

- EMBL:   U55242
- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   G70527
- RefSeq:   NP_214848.1
- RefSeq:   NP_334757.1
- HSSP:   Q9HU22
- ProteinModelPortal:   P72017
- SMR:   P72017
- EnsemblBacteria:   EBMYCT00000003583
- EnsemblBacteria:   EBMYCT00000071812
- GeneID:   886568
- GeneID:   923474
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0348
- KEGG:   mtu:Rv0334
- TIGR:   MT0348
- TubercuList:   Rv0334
- GeneTree:   EBGT00050000016064
- HOGENOM:   HBG688195
- OMA:   INRIYME
- ProtClustDB:   CLSK2517373
- GO:   GO:0040007
- InterPro:   IPR005907
- InterPro:   IPR005835
- TIGRFAMs:   TIGR01207

Pfam domain/function: PF00483 NTP_transferase

EC number: =2.7.7.24

Molecular weight: Translated: 31492; Mature: 31492

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLTTLMMAGIRDIQLITTPHDAPG
CCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHCCCCEEEEEECCCCCCC
FHRLLGDGAHLGVNISYATQDQPDGLAQAFVIGANHIGADSVALVLGDNIFYGPGLGTSL
HHHHHCCCCEEEEEEEECCCCCCCCHHEEEEEECCCCCCCCEEEEECCCEEECCCCCHHH
KRFQSISGGAIFAYWVANPSAYGVVEFGAEGMALSLEEKPVTPKSNYAVPGLYFYDNDVI
HHHHCCCCCEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCHH
EIARGLKKSARGEYEITEVNQVYLNQGRLAVEVLARGTAWLDTGTFDSLLDAADFVRTLE
HHHHHHHHHCCCCEEEEEHHEEECCCCCEEEEEHHCCCEEEECCCHHHHHHHHHHHHHHH
RRQGLKVSIPEEVAWRMGWIDDEQLVQRARALVKSGYGNYLLELLERN
HHCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MRGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLTTLMMAGIRDIQLITTPHDAPG
CCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHCCCCEEEEEECCCCCCC
FHRLLGDGAHLGVNISYATQDQPDGLAQAFVIGANHIGADSVALVLGDNIFYGPGLGTSL
HHHHHCCCCEEEEEEEECCCCCCCCHHEEEEEECCCCCCCCEEEEECCCEEECCCCCHHH
KRFQSISGGAIFAYWVANPSAYGVVEFGAEGMALSLEEKPVTPKSNYAVPGLYFYDNDVI
HHHHCCCCCEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEECCCHH
EIARGLKKSARGEYEITEVNQVYLNQGRLAVEVLARGTAWLDTGTFDSLLDAADFVRTLE
HHHHHHHHHCCCCEEEEEHHEEECCCCCEEEEEHHCCCEEEECCCHHHHHHHHHHHHHHH
RRQGLKVSIPEEVAWRMGWIDDEQLVQRARALVKSGYGNYLLELLERN
HHCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084178; 9634230; 12218036