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The map label for this gene is dnaK [C]

Identifier: 148660078

GI number: 148660078

Start: 381918

End: 383780

Strand: Direct

Name: dnaK [C]

Synonym: MRA_0321

Alternate gene names: 148660078

Gene position: 381918-383780 (Clockwise)

Preceding gene: 148660077

Following gene: 148660079

Centisome position: 8.64

GC content: 65.7

Gene sequence:

>1863_bases
ATGTACGACCCGCTGGGGTTGTCGATCGGGACCACAAACCTGGTCGCGGCGGGTAACGGAGGTCCGCCGGTTACTCGTCG
CGCCGTGCTGACCCTGTACCCGCATTGCGCACCGAAAATCGGTGTGCCTAGCCAGAACCCGAACTTGATCGAGCCGGGCG
CCCTAATGAGCGGCTTTGTTGAGCGCATTGGAGATGCGGTGGCGCTGGTGTCTCCCGACGGATCCGTGCACGATCCAGAC
CTCTTGCTGGTCGAGGCGCTGGATGCGATGGTGCTGACCGCCGGTGCGGACGCGAGTTCCTCGGAGATCGCCATTGCCGT
TCCCGCGCATTGGAAGCCCGGAGCTGTACACGCACTGCGTAACGGTTTGCGGACGCACGTCGGCTTCGTCCGCAGCGGCA
TGGCGCCGCGCCTGGTTTCCGATGCGATCGCGGCGTTGACCGCGGTGAACTCGGAATTGGGCCTGCCCCACGGCAGTGTG
GTGGGGTTGCTTGATTTCGGTGGCTCCGCGACTTACGTCACCTTGGTGGAGACCAAGTCGGATTCCAGGACGTCGGATTT
CCAGCCCGTTAGTGCCACGGCACGGTACCAGGACTTTTCCGGTAGTCAGATCGACCAGGCTTTGCTGCTTCGGGTCATCG
ACCAATTCGGGTACGGCGATGACGTCGATCCGGCCAGTACCGCCGCGGTCGGGCAACTCGGCCAACTCAGGGAGCAGTGC
CGTGCGGCAAAGGAACGACTGTCCACCGACGTTGCCACGGAATTGTTCGCTGAGCTTGCCGGGTGCAGCTCGAGCATCGA
GATGACTCGGGAACAGCTCGAAGACCTGATCCAGGATCCATTGACCGGCTTCATCTACGCGTTCGACGACATGCTGGCGC
GCCACAACGCGAGCTGGGCGGATCTCGCGGCGGTGGTCACCGTCGGCGGTGGTGCCAATATTCCCCTTGTGACTCAACGT
CTTTCGTTCCACACTCGTCGACCTGTGCTGACCGCGTCGCAACCCGGGTGCGCGGCGGCGATGGGTGCGTTGCTGCTCGC
CAACCGTGGGGGAGAGCGCGATTCGCGAACGCGGACGTCCATCGGCCTCGCCACGGCCGCAGCCGCCGGCACCAGTGTCA
TCGAGCTGCCGGCCGGCGACGTCATGGTCATCGACCATGAGGCCTTGACCGATCGCGAGTTGGCCTGGTCGCAGACCGAC
TTCCCAAGCGAAGCTCCGGCGCGTTTCGAGGGCGACTCGTATAACGAAGGCGGCCCCTGCTGGTCGATGCGTCTGAACGC
GGTCGAGCCCCCCAAAGGACCAGCGTGGCGGCGAATCCGGGTGTCGCAGTTGCTCATCGGGGTGTCGGCGGTAGTGGCCA
TGACCGCGATCGGGGGCGTGGCATTGACGTTGACAGCCATCGAGAGACGCCCAAGCCCGCTACCAACCCCAATTGTGCCC
GGCCTGGCCCCGATGCCGCCCGGATCCGTCGTGCCTAGCTCGCGCGCACCGACCCCGCCGCCACCGCCGTCGACCGTTGC
GCCGCTTCCCAGTGCGGCACCGGCCCCGACGACGGTCGCGCCGGCACCGCCGCCGCCCACACAGGTGGTGACGACCACGA
CAGCGCCACCCGTCACCACGACGCCGAGGCCGTCGCCGACCACCACAACGACCACCGCGCCACCGTCGACAACGACGACA
ACCGAGCCGCCGGTGACGACCACTTCGACGATTCCAACGATTCCGACGACTACGACGACGGTGAAGATGACCACGGAGTG
GTTGCACGTCCCGTTTTTGCCCGTTCCGATCCCGGTCCCGATTCCGCAAAATCCGGGTGCCGGCGAACCGCAGAACCCGT
TCGGAAGCCTTGGCTCTGGGTGA

Upstream 100 bases:

>100_bases
TCGACCGCATACCAATGAGGTTGTCACCGAACCGTATACGGCCCACCCGCCGCTATGGTTAACGCTGGCCACCGACCCCT
ATTGACGAAAGCCTTCCGCT

Downstream 100 bases:

>100_bases
GCCGCGTTCCCCGGAGCTGGCCCCGTCGGTGTCAGGTCCGTAGTATCGGTATGGGTTGCTGAGGAGGTCGCGTGGGCGAC
TATGGTCCGTTTGGATTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: None

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFVERIGDAVALVSPDGSVHDPD
LLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALRNGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGSV
VGLLDFGGSATYVTLVETKSDSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWADLAAVVTVGGGANIPLVTQR
LSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTSIGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTD
FPSEAPARFEGDSYNEGGPCWSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTTTPRPSPTTTTTTAPPSTTTT
TEPPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVPIPQNPGAGEPQNPFGSLGSG

Sequences:

>Translated_620_residues
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFVERIGDAVALVSPDGSVHDPD
LLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALRNGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGSV
VGLLDFGGSATYVTLVETKSDSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWADLAAVVTVGGGANIPLVTQR
LSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTSIGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTD
FPSEAPARFEGDSYNEGGPCWSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTTTPRPSPTTTTTTAPPSTTTT
TEPPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVPIPQNPGAGEPQNPFGSLGSG
>Mature_620_residues
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFVERIGDAVALVSPDGSVHDPD
LLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALRNGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGSV
VGLLDFGGSATYVTLVETKSDSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWADLAAVVTVGGGANIPLVTQR
LSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTSIGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTD
FPSEAPARFEGDSYNEGGPCWSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTTTPRPSPTTTTTTAPPSTTTT
TEPPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVPIPQNPGAGEPQNPFGSLGSG

Specific function: Plays An Essential Role In The Initiation Of Phage Lambda DNA Replication, Where It Acts In An ATP-Dependent Fashion With The DNAj Protein To Release Lambda O And P Proteins From The Preprimosomal Complex. DNAk Is Also Involved In Chromosomal DNA Replica

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 64144; Mature: 64144

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFV
CCCCCCCEECCCEEEEECCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
ERIGDAVALVSPDGSVHDPDLLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALR
HHHCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCCCHHHHHH
NGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGSVVGLLDFGGSATYVTLVETKS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECC
DSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHH
DLAAVVTVGGGANIPLVTQRLSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTS
HEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHH
IGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTDFPSEAPARFEGDSYNEGGPC
HHHHHHHHCCCEEEEECCCCEEEEECHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCE
WSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCC
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
TPRPSPTTTTTTAPPSTTTTTEPPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
IPQNPGAGEPQNPFGSLGSG
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFV
CCCCCCCEECCCEEEEECCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
ERIGDAVALVSPDGSVHDPDLLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALR
HHHCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCCCHHHHHH
NGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGSVVGLLDFGGSATYVTLVETKS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEECC
DSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHH
DLAAVVTVGGGANIPLVTQRLSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTS
HEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHH
IGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTDFPSEAPARFEGDSYNEGGPC
HHHHHHHHCCCEEEEECCCCEEEEECHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCE
WSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCC
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
TPRPSPTTTTTTAPPSTTTTTEPPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
IPQNPGAGEPQNPFGSLGSG
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA