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The map label for this gene is nirD [H]

Identifier: 148660018

GI number: 148660018

Start: 306815

End: 307171

Strand: Direct

Name: nirD [H]

Synonym: MRA_0262

Alternate gene names: 148660018

Gene position: 306815-307171 (Clockwise)

Preceding gene: 148660017

Following gene: 148660022

Centisome position: 6.94

GC content: 64.71

Gene sequence:

>357_bases
GTGACGCTTCTCAACGACATTCAGGTATGGACCACCGCCTGCGCATACGACCATCTCATTCCGGGACGTGGTGTCGGGGT
GTTACTCGATGACGGTAGTCAGGTGGCACTGTTCCGGCTCGACGACGGCTCGGTGCACGCGGTCGGTAACGTCGACCCGT
TCTCCGGTGCTGCGGTGATGTCCCGCGGCATCGTCGGTGATCGCGGAGGTCGCGCCATGGTGCAATCGCCGATCCTGAAG
CAGGCTTTCGCGCTCGACGATGGCTCGTGCCTCGACGATCCGCGCGTTTCGGTGCCGGTGTATCCGGCGCGCGTCACACC
CGAAGGCCGCATTCAGGTCGCGCGGGTAGCGGTCTAG

Upstream 100 bases:

>100_bases
TCGACGGTGACCTTCACCGAGCGTGCCGGGCGCAAAGTACCTGTGTCCATTGGTATCCCGCGGGTCCGATCATGAAGTCC
GGGAGGACAAAGGAGGGACT

Downstream 100 bases:

>100_bases
CTCACCCCGCGAACCTCACAGCTTGAGCACACGTCCGGCGATGACCAGATGTACCTCATCGCAGACGGCTGCCACGCGTC
GGTTGATTGTGCCCAGTAGA

Product: putative nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MTLLNDIQVWTTACAYDHLIPGRGVGVLLDDGSQVALFRLDDGSVHAVGNVDPFSGAAVMSRGIVGDRGGRAMVQSPILK
QAFALDDGSCLDDPRVSVPVYPARVTPEGRIQVARVAV

Sequences:

>Translated_118_residues
MTLLNDIQVWTTACAYDHLIPGRGVGVLLDDGSQVALFRLDDGSVHAVGNVDPFSGAAVMSRGIVGDRGGRAMVQSPILK
QAFALDDGSCLDDPRVSVPVYPARVTPEGRIQVARVAV
>Mature_117_residues
TLLNDIQVWTTACAYDHLIPGRGVGVLLDDGSQVALFRLDDGSVHAVGNVDPFSGAAVMSRGIVGDRGGRAMVQSPILKQ
AFALDDGSCLDDPRVSVPVYPARVTPEGRIQVARVAV

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=98, Percent_Identity=42.8571428571429, Blast_Score=83, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 12430; Mature: 12299

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLNDIQVWTTACAYDHLIPGRGVGVLLDDGSQVALFRLDDGSVHAVGNVDPFSGAAVM
CCCCCCHHEEHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCEEEECCCCCCCCHHHH
SRGIVGDRGGRAMVQSPILKQAFALDDGSCLDDPRVSVPVYPARVTPEGRIQVARVAV
HCCCCCCCCCCCCHHCHHHHHHHCCCCCCCCCCCCCCCCEECEEECCCCCEEEEEEEC
>Mature Secondary Structure 
TLLNDIQVWTTACAYDHLIPGRGVGVLLDDGSQVALFRLDDGSVHAVGNVDPFSGAAVM
CCCCCHHEEHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCEEEECCCCCCCCHHHH
SRGIVGDRGGRAMVQSPILKQAFALDDGSCLDDPRVSVPVYPARVTPEGRIQVARVAV
HCCCCCCCCCCCCHHCHHHHHHHCCCCCCCCCCCCCCCCEECEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]