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The map label for this gene is pbpA

Identifier: 148659775

GI number: 148659775

Start: 20117

End: 21592

Strand: Reverse

Name: pbpA

Synonym: MRA_0018

Alternate gene names: 148659775

Gene position: 21592-20117 (Counterclockwise)

Preceding gene: 148659776

Following gene: 148659774

Centisome position: 0.49

GC content: 64.5

Gene sequence:

>1476_bases
ATGAACGCCTCTCTGCGCCGAATATCGGTGACCGTGATGGCGTTGATCGTGTTGCTACTGCTCAACGCGACCATGACGCA
GGTCTTCACCGCCGACGGGCTGCGTGCCGATCCCCGCAACCAGCGAGTGTTGCTCGACGAGTATTCACGGCAGCGCGGCC
AGATCACCGCTGGTGGCCAACTGCTGGCGTACTCGGTAGCCACCGACGGCCGCTTTCGTTTCCTGCGGGTCTATCCCAAT
CCTGAGGTGTACGCGCCGGTTACCGGCTTCTACTCCCTGCGCTATTCCAGCACCGCCCTAGAACGAGCCGAGGACCCGAT
ATTGAACGGGTCCGACCGCCGTCTGTTCGGCCGCCGGCTGGCCGACTTCTTCACCGGTCGCGACCCACGCGGCGGTAATG
TCGATACCACGATCAACCCGCGCATTCAGCAAGCCGGCTGGGACGCGATGCAGCAAGGCTGCTACGGGCCCTGTAAGGGA
GCGGTGGTCGCCCTTGAGCCATCAACCGGCAAGATTTTGGCGTTGGTGTCTTCTCCGTCCTACGACCCCAACCTGCTGGC
GTCGCATAACCCCGAGGTGCAGGCGCAAGCCTGGCAGCGGCTTGGCGACAATCCCGCCTCTCCACTGACCAACCGTGCCA
TCTCTGAGACGTATCCACCGGGTTCGACTTTCAAAGTGATCACCACTGCGGCCGCGCTGGCCGCCGGGGCCACCGAGACC
GAACAGCTGACTGCGGCGCCCACAATTCCGTTGCCAGGCAGCACCGCCCAGCTAGAGAACTACGGCGGTGCGCCGTGCGG
GGACGAACCCACCGTGTCGCTGCGTGAGGCATTCGTCAAATCATGCAACACCGCATTCGTCCAGCTGGGCATCCGCACCG
GCGCCGACGCCCTGCGCAGCATGGCGCGCGCGTTCGGTCTCGATAGCCCACCGCGCCCAACTCCGCTGCAAGTGGCGGAA
TCAACCGTCGGGCCTATCCCGGACAGCGCCGCACTAGGGATGACCAGTATCGGCCAAAAGGACGTTGCGCTGACCCCGCT
AGCGAACGCAGAAATAGCCGCGACCATCGCAAACGGCGGCATTACGATGAGGCCTTATCTAGTCGGCAGCCTCAAGGGAC
CGGACCTAGCCAATATCTCAACCACCGTCGGATACCAGCAGCGCCGCGCGGTGTCACCGCAGGTCGCCGCTAAGCTAACA
GAGCTGATGGTCGGCGCCGAGAAAGTCGCACAGCAGAAAGGGGCAATCCCCGGCGTGCAGATCGCATCCAAGACGGGCAC
CGCCGAACATGGCACCGACCCTCGTCACACTCCACCGCACGCTTGGTACATCGCCTTTGCGCCCGCACAAGCGCCCAAGG
TGGCTGTTGCCGTGCTGGTGGAGAACGGGGCTGATCGGCTGTCCGCCACCGGAGGTGCCCTCGCGGCACCGATCGGGCGG
GCGGTGATCGAAGCCGCACTGCAGGGGGAACCATGA

Upstream 100 bases:

>100_bases
TCCTGGCACGCATCTCGCACGGAGCCCGCCGCCCACTGCGCACCCGCCCACGAAATAAGTCGCCGATTACGGCGGCCGGC
ACCGAGGTCATCGAACGCGT

Downstream 100 bases:

>100_bases
GCCCCCGAGTTGGCGTGACGCTGTCGGGCAGATACCGCCTGCAGCGCCTCATCGCCACCGGTGGTATGGGCCAAGTCTGG
GAGGCCGTGGATAACCGGTT

Product: putative penicillin-binding protein PbpA

Products: NA

Alternate protein names: PBPA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDGRFRFLRVYPN
PEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKG
AVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATET
EQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAE
STVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLT
ELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGR
AVIEAALQGEP

Sequences:

>Translated_491_residues
MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDGRFRFLRVYPN
PEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKG
AVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATET
EQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAE
STVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLT
ELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGR
AVIEAALQGEP
>Mature_491_residues
MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDGRFRFLRVYPN
PEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKG
AVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATET
EQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAE
STVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLT
ELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGR
AVIEAALQGEP

Specific function: Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Probable). Note=Localized to the division site (septum) along with newly synthesized peptidoglycan, between segregated nucleoids

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786854, Length=376, Percent_Identity=27.6595744680851, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786272, Length=436, Percent_Identity=25.6880733944954, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPA_MYCTU (P71586)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842572
- PIR:   F70699
- RefSeq:   NP_214530.1
- RefSeq:   NP_334427.1
- PDB:   3LO7
- PDBsum:   3LO7
- ProteinModelPortal:   P71586
- PhosSite:   P71586
- EnsemblBacteria:   EBMYCT00000003488
- EnsemblBacteria:   EBMYCT00000072473
- GeneID:   887078
- GeneID:   922467
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0019
- KEGG:   mtu:Rv0016c
- TIGR:   MT0019
- TubercuList:   Rv0016c
- GeneTree:   EBGT00050000015310
- HOGENOM:   HBG403509
- OMA:   GATENDQ
- ProtClustDB:   CLSK790194
- GO:   GO:0005829
- InterPro:   IPR012338
- InterPro:   IPR001460

Pfam domain/function: PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 51576; Mature: 51576

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: ACT_SITE 222-222

Signals:

None

Transmembrane regions:

HASH(0x18a544fc)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCEEECCE
LLAYSVATDGRFRFLRVYPNPEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRL
EEEEEEECCCCEEEEEECCCCCEEECCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHHH
ADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKGAVVALEPSTGKILALVSSPS
HHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCC
YDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATET
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
EQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRS
HHEECCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHH
MARAFGLDSPPRPTPLQVAESTVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGG
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHCCHHHCCCCCEEECCCCCCEEEEEEECCC
ITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLTELMVGAEKVAQQKGAIPGVQ
EEECCEEEECCCCCCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGR
EECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCHHHHCCCCHHHHHHHH
AVIEAALQGEP
HHHHHHHCCCH
>Mature Secondary Structure
MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCEEECCE
LLAYSVATDGRFRFLRVYPNPEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRL
EEEEEEECCCCEEEEEECCCCCEEECCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHHH
ADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKGAVVALEPSTGKILALVSSPS
HHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCC
YDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATET
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
EQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRS
HHEECCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHH
MARAFGLDSPPRPTPLQVAESTVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGG
HHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHCCHHHCCCCCEEECCCCCCEEEEEEECCC
ITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLTELMVGAEKVAQQKGAIPGVQ
EEECCEEEECCCCCCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGR
EECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCHHHHCCCCHHHHHHHH
AVIEAALQGEP
HHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036