Click here to switch to the map view.

The map label for this gene is mnmC

Identifier: 148653786

GI number: 148653786

Start: 2476196

End: 2478415

Strand: Direct

Name: mnmC

Synonym: PsycPRwf_1989

Alternate gene names: 148653786

Gene position: 2476196-2478415 (Clockwise)

Preceding gene: 148653785

Following gene: 148653792

Centisome position: 83.12

GC content: 48.65

Gene sequence:

>2220_bases
ATGGATAAGGTCACCCCTGCCAAGCTGTCTTGGCGTGAAGATGAACTCGGTAACGTGGTCCCCGTCTCTGAGATATTCGG
CGATGTGTATTACTCTTTGGTCGATGGATTAAATGAGTCGCGTTATGTATTTTTGACCCAAAATAAATTACCTGAGCGCT
TTGAAGCCTTATTTAGCCGAGCGAATAGTAGCGCAGCGTCTAACTTTAGCATTGCTGAATTAGGCTTTGGTACCGGTCTT
AATATACTGGCCACTTGGCAGCTTTGGGAACACAGTAAGCAGCAGTTCCATACTTATTTCAACTCACAAACTAACCTGCA
AACGAGCTCGCAAGATCATTTAAAGGGCTCACGCTCGCTAACACCGCGGCTGCATATTATCAGTACCGAAAAGCATCCGC
TCACTCATGCCGATTTAAGCCGCAGTCTTGAAAGCTGGAAGCATAAAGACACCAGTCTGGTGCCCTTTGTCGATCAGCTA
CTCGCCTTATATCCCACCTTGATATCTGGCTGTCATCGCCTACAGTTGGCCGAAGATGTCACGTTAGATTTATGGCTTGG
TGATGCCTGTTTCAGCTTACAACAATTGGCCAATAGCTATGACACGCTGCCCTACGGCGCTCACATTGATGCTTGGTACT
TAGATGGCTTTGCGCCTGCATGTAATGAGTCACTTTGGGCAGATCAGATCTTTGAGCAGGTAAAGCGCTTATCTAAGCCT
GGTACTACTGCGGCGACCTTTAGCAGTGCTGGCGTGGTCAAACGCGGCTTGATGGCGGCTGGCTTTGAGATCAAAAAAAC
CAAAGGCTTTGGACGCAAACGTGAAATGCTAACCGCCACCAAGCTTGAGCCGGTAGCAGTCAGTGACGATACCAGTGACA
ACCAAGGTGCTACTGAGTATGATGCCAAGTCAACTTTAGCATTACGCTCTAAGGAGCAAGTCACACTGGATACACATGCC
GTGACGCCCAACGAGCAGATTGCAGTGATTGGTGCAGGTGTTTGTGGATTAATGGCGGCTTGGTCATTGGCGCAGCGTGG
TAATGCGGTGAGCTTAATAGACAAAGAGGCCCCGCTAGCGGGTGCCTCTGGCAACCCAAGGGCGCTGCTTGCTCCTAAGA
TGACGCCGCTTGCTCATGTGGCTGAGCATTTGCACAGCATCAGCTACCTTTATAGCCAACGCTTTTATCGTCAAATCGAT
CAAGGTGAGCATAAAATATTTACCCCCACCACCACCTTAGACTTGCTACAAAAAAGCAACGTCGATGTGCACCAAATCGC
TGAATATCCTGAGCAAATGGCCACCACCTTATCACTTGATAGCGCCCGAGATATATCTGGTTTACAGCAGCAGGATTTGA
CAGCGAATTTATACCTGCCTCAATCAGGGCTTATTAACCCTAAGGCACTGGCCGATAAGGTACTTACCCACCCAAATATC
AGCTTTAAACAAGCGAACATTAAGCGCATTACCCCCTTACAGGCAGAGGCTTTTGATGACGTAACTGAAGTAAGTGAAGT
AATTGAAGTCACTGCGGATATAACGGTGCAGCCTGCTAAGCGTACTAAAGTTATGCTGCACTGCGAAAGTGGAGAGGTGT
TGTCGGTCAATAGAGTGGTTATTGCTGCCGCTTTTGAAAGTCAAAGCTTGGATGCGCGCATTTTTGAGTTTCGTAAAATT
CGGGGTCAATTATCATGGTTTGAGCCCACTACCGAGCAGCTGCAAGCACTGCCAACCTTACCACTAAAATACTCAGGCTA
CTGCGCCCCTTTTATACCGCAGCCCGGTGATGAAGCCGTTAACTCAGTCACGCCCTCTGTGCCTACTTTTTTGCTTGGGG
CGAGCTTTATTCGCAATGATTTGGATGATGAAATTCGGCTTAGTGAGCATGCGATTAACCATCAAAAGCTACTAACGGCG
CTGCCTGAGCTTTCGGCGGTTCTGCCTTTACCGGATACAGAGGCTGACTTATATCAGCGATGGCAGGCACGAGTGGGTAT
TCGATCACAGACGCCAGATTATCACCCTTTGGTGGGTGCAGTCGATGAGCAGGGTCTGATTTGGACACTGAGCGGCATGG
GCTCTAAGGGCTATGCTTTTGCACCGCTATGTGCTCAGGTATTAGCTGATATGATGACCGCACAGTTTGTACCGCTGCCG
GCGGCGCTGCTCGCTAAGCTATCAGTGCATCGAAGCAGTTTAAAAAAGCCGTTATCATAA

Upstream 100 bases:

>100_bases
TTTATCTTTAGTCGCCGTGACCGCTGGTAATGGGCACTCTTAAAGTTAAGGCAAGATTTGGTACAAAAACCATAACCCAA
GCAATAACATAAGGCACAAA

Downstream 100 bases:

>100_bases
CGGCTTTTTTTGATGTTTGTTTTATGATGGCTACCAAGACTAGCGGCTTAGCATCGCTAACGTCTCACTGACCATGACCA
TACCCACCACCGAAGTGACG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MDKVTPAKLSWREDELGNVVPVSEIFGDVYYSLVDGLNESRYVFLTQNKLPERFEALFSRANSSAASNFSIAELGFGTGL
NILATWQLWEHSKQQFHTYFNSQTNLQTSSQDHLKGSRSLTPRLHIISTEKHPLTHADLSRSLESWKHKDTSLVPFVDQL
LALYPTLISGCHRLQLAEDVTLDLWLGDACFSLQQLANSYDTLPYGAHIDAWYLDGFAPACNESLWADQIFEQVKRLSKP
GTTAATFSSAGVVKRGLMAAGFEIKKTKGFGRKREMLTATKLEPVAVSDDTSDNQGATEYDAKSTLALRSKEQVTLDTHA
VTPNEQIAVIGAGVCGLMAAWSLAQRGNAVSLIDKEAPLAGASGNPRALLAPKMTPLAHVAEHLHSISYLYSQRFYRQID
QGEHKIFTPTTTLDLLQKSNVDVHQIAEYPEQMATTLSLDSARDISGLQQQDLTANLYLPQSGLINPKALADKVLTHPNI
SFKQANIKRITPLQAEAFDDVTEVSEVIEVTADITVQPAKRTKVMLHCESGEVLSVNRVVIAAAFESQSLDARIFEFRKI
RGQLSWFEPTTEQLQALPTLPLKYSGYCAPFIPQPGDEAVNSVTPSVPTFLLGASFIRNDLDDEIRLSEHAINHQKLLTA
LPELSAVLPLPDTEADLYQRWQARVGIRSQTPDYHPLVGAVDEQGLIWTLSGMGSKGYAFAPLCAQVLADMMTAQFVPLP
AALLAKLSVHRSSLKKPLS

Sequences:

>Translated_739_residues
MDKVTPAKLSWREDELGNVVPVSEIFGDVYYSLVDGLNESRYVFLTQNKLPERFEALFSRANSSAASNFSIAELGFGTGL
NILATWQLWEHSKQQFHTYFNSQTNLQTSSQDHLKGSRSLTPRLHIISTEKHPLTHADLSRSLESWKHKDTSLVPFVDQL
LALYPTLISGCHRLQLAEDVTLDLWLGDACFSLQQLANSYDTLPYGAHIDAWYLDGFAPACNESLWADQIFEQVKRLSKP
GTTAATFSSAGVVKRGLMAAGFEIKKTKGFGRKREMLTATKLEPVAVSDDTSDNQGATEYDAKSTLALRSKEQVTLDTHA
VTPNEQIAVIGAGVCGLMAAWSLAQRGNAVSLIDKEAPLAGASGNPRALLAPKMTPLAHVAEHLHSISYLYSQRFYRQID
QGEHKIFTPTTTLDLLQKSNVDVHQIAEYPEQMATTLSLDSARDISGLQQQDLTANLYLPQSGLINPKALADKVLTHPNI
SFKQANIKRITPLQAEAFDDVTEVSEVIEVTADITVQPAKRTKVMLHCESGEVLSVNRVVIAAAFESQSLDARIFEFRKI
RGQLSWFEPTTEQLQALPTLPLKYSGYCAPFIPQPGDEAVNSVTPSVPTFLLGASFIRNDLDDEIRLSEHAINHQKLLTA
LPELSAVLPLPDTEADLYQRWQARVGIRSQTPDYHPLVGAVDEQGLIWTLSGMGSKGYAFAPLCAQVLADMMTAQFVPLP
AALLAKLSVHRSSLKKPLS
>Mature_739_residues
MDKVTPAKLSWREDELGNVVPVSEIFGDVYYSLVDGLNESRYVFLTQNKLPERFEALFSRANSSAASNFSIAELGFGTGL
NILATWQLWEHSKQQFHTYFNSQTNLQTSSQDHLKGSRSLTPRLHIISTEKHPLTHADLSRSLESWKHKDTSLVPFVDQL
LALYPTLISGCHRLQLAEDVTLDLWLGDACFSLQQLANSYDTLPYGAHIDAWYLDGFAPACNESLWADQIFEQVKRLSKP
GTTAATFSSAGVVKRGLMAAGFEIKKTKGFGRKREMLTATKLEPVAVSDDTSDNQGATEYDAKSTLALRSKEQVTLDTHA
VTPNEQIAVIGAGVCGLMAAWSLAQRGNAVSLIDKEAPLAGASGNPRALLAPKMTPLAHVAEHLHSISYLYSQRFYRQID
QGEHKIFTPTTTLDLLQKSNVDVHQIAEYPEQMATTLSLDSARDISGLQQQDLTANLYLPQSGLINPKALADKVLTHPNI
SFKQANIKRITPLQAEAFDDVTEVSEVIEVTADITVQPAKRTKVMLHCESGEVLSVNRVVIAAAFESQSLDARIFEFRKI
RGQLSWFEPTTEQLQALPTLPLKYSGYCAPFIPQPGDEAVNSVTPSVPTFLLGASFIRNDLDDEIRLSEHAINHQKLLTA
LPELSAVLPLPDTEADLYQRWQARVGIRSQTPDYHPLVGAVDEQGLIWTLSGMGSKGYAFAPLCAQVLADMMTAQFVPLP
AALLAKLSVHRSSLKKPLS

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=766, Percent_Identity=29.3733681462141, Blast_Score=270, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PSYWF (A5WGY8)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280879.1
- ProteinModelPortal:   A5WGY8
- SMR:   A5WGY8
- STRING:   A5WGY8
- GeneID:   5205943
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1989
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   CATANAG
- ProtClustDB:   CLSK830736
- BioCyc:   PSP56811:PSYCPRWF_1989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 81423; Mature: 81423

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVTPAKLSWREDELGNVVPVSEIFGDVYYSLVDGLNESRYVFLTQNKLPERFEALFSR
CCCCCCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
ANSSAASNFSIAELGFGTGLNILATWQLWEHSKQQFHTYFNSQTNLQTSSQDHLKGSRSL
CCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCC
TPRLHIISTEKHPLTHADLSRSLESWKHKDTSLVPFVDQLLALYPTLISGCHRLQLAEDV
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLDLWLGDACFSLQQLANSYDTLPYGAHIDAWYLDGFAPACNESLWADQIFEQVKRLSKP
EEEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHCCC
GTTAATFSSAGVVKRGLMAAGFEIKKTKGFGRKREMLTATKLEPVAVSDDTSDNQGATEY
CCCHHHCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCC
DAKSTLALRSKEQVTLDTHAVTPNEQIAVIGAGVCGLMAAWSLAQRGNAVSLIDKEAPLA
CCHHHHEECCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCC
GASGNPRALLAPKMTPLAHVAEHLHSISYLYSQRFYRQIDQGEHKIFTPTTTLDLLQKSN
CCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCC
VDVHQIAEYPEQMATTLSLDSARDISGLQQQDLTANLYLPQSGLINPKALADKVLTHPNI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
SFKQANIKRITPLQAEAFDDVTEVSEVIEVTADITVQPAKRTKVMLHCESGEVLSVNRVV
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECCCCEEEECEEE
IAAAFESQSLDARIFEFRKIRGQLSWFEPTTEQLQALPTLPLKYSGYCAPFIPQPGDEAV
EEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
NSVTPSVPTFLLGASFIRNDLDDEIRLSEHAINHQKLLTALPELSAVLPLPDTEADLYQR
HHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHH
WQARVGIRSQTPDYHPLVGAVDEQGLIWTLSGMGSKGYAFAPLCAQVLADMMTAQFVPLP
HHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
AALLAKLSVHRSSLKKPLS
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKVTPAKLSWREDELGNVVPVSEIFGDVYYSLVDGLNESRYVFLTQNKLPERFEALFSR
CCCCCCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHH
ANSSAASNFSIAELGFGTGLNILATWQLWEHSKQQFHTYFNSQTNLQTSSQDHLKGSRSL
CCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCC
TPRLHIISTEKHPLTHADLSRSLESWKHKDTSLVPFVDQLLALYPTLISGCHRLQLAEDV
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLDLWLGDACFSLQQLANSYDTLPYGAHIDAWYLDGFAPACNESLWADQIFEQVKRLSKP
EEEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHCCC
GTTAATFSSAGVVKRGLMAAGFEIKKTKGFGRKREMLTATKLEPVAVSDDTSDNQGATEY
CCCHHHCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCC
DAKSTLALRSKEQVTLDTHAVTPNEQIAVIGAGVCGLMAAWSLAQRGNAVSLIDKEAPLA
CCHHHHEECCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCC
GASGNPRALLAPKMTPLAHVAEHLHSISYLYSQRFYRQIDQGEHKIFTPTTTLDLLQKSN
CCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCC
VDVHQIAEYPEQMATTLSLDSARDISGLQQQDLTANLYLPQSGLINPKALADKVLTHPNI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
SFKQANIKRITPLQAEAFDDVTEVSEVIEVTADITVQPAKRTKVMLHCESGEVLSVNRVV
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECCCCEEEECEEE
IAAAFESQSLDARIFEFRKIRGQLSWFEPTTEQLQALPTLPLKYSGYCAPFIPQPGDEAV
EEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
NSVTPSVPTFLLGASFIRNDLDDEIRLSEHAINHQKLLTALPELSAVLPLPDTEADLYQR
HHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHH
WQARVGIRSQTPDYHPLVGAVDEQGLIWTLSGMGSKGYAFAPLCAQVLADMMTAQFVPLP
HHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
AALLAKLSVHRSSLKKPLS
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA