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The map label for this gene is macA [H]

Identifier: 148653630

GI number: 148653630

Start: 2276992

End: 2278386

Strand: Direct

Name: macA [H]

Synonym: PsycPRwf_1833

Alternate gene names: 148653630

Gene position: 2276992-2278386 (Clockwise)

Preceding gene: 148653629

Following gene: 148653631

Centisome position: 76.44

GC content: 48.53

Gene sequence:

>1395_bases
ATGGCCTTCATTTCCAATCAAAAAACTCGAAAGTCTGTTATTAAATGGACCATTATTGCCCTTATTGCGCTCGCTTTGGC
TGTCTTAGCTTATAACAAATTTAAACCCAAACAAGAAGAAACCAATTATATTACTGCCACCGCTGAGATGGGTGATGTTG
AAAATAACGTCATGGCATCAGGCAAGGTCAAAGCCTTGAATACAGTAGATGTAGGTGCCCAGGTCTCAGGTGAAGTGACT
AAGCTATTTGTGGATGTGGGTGATGAGGTCAAAAAAGGCGACTTAATTGCCCAAATCGACCAAGTCACTCAAAAGAACAA
TCTTTCAAATCAGCAGGCCACCTTAGAGCAAGGCCGCGCCGCGCTTCAAAGTGCTCAAGCAGACTACTATAGCCGCAAAG
CAGGATTAGAGAGTGCCCAGGCCGATCTTGCCAGCCGAGAGGCAGACTTAAAACAGGCTCAATCTGATTATGCCAGACTA
CGTCCACTACTTGCGATGGATGCCATCAGTCAGCAAGAGGTGGAGAATGCCCGTACCACTGTCGAAAAAGCTCAAGCCAG
TGTGGCCGCCGCAAAAGCAGCTATTAAAACCGCTCAAGCGGCACTCATTACCGCTCAGTCCAATATCGCAAGCAGTGAAG
CTGACGTGCGAAAAGCCCAAACCAACTTAGACACTGCCAGTACAGACTTAGGCTACACCACCATTCGTGCACCCATGGAT
GGCACCGTCGTGGCTGTGGTCACCGAACAAGGTACAACGGTAAATGCCAATCAAGCGGCACCCACCATCGTCAAATTAGC
CGACTTATCAACCGTCCGTATTAATGCGCAAATCTCAGAGGCAGATGTCATTAACGTAAAAGCCGGTATGCCTGTGTACT
TTAATACCATCGGTGATCCGGACAAAAAATACGATGCAATCTTAACTGCCGTTGAGCCTGCACCGGAACAAATCAGTGAT
ACCAGCTCCACCGACTCGGCGATTTACTATGTTGGTTATATTGAAGTGCCCAACCCAGAGCGTCGTTTTCGTATTGATAT
GACCGCACAGGTCTATGTGATCATTGATAAGGCCACCAATACCTTGCTTGTACCCTCTGCTGCGATCAAAAAAGGCCCTA
ACAAAGGCGCTTCTGGCGCAGCAAGATCTAGATCTGGCGCAGGCGGCAACAATAAGCCAGCTTCAGCCGCTCCAGCACCA
GTCAAAGGTCCCTACGTGCGCGTGCTTAATGCCGATGGTAGTGTAGAAAATCGTGCGGTCAAAGTAGGTATTGATAACCG
AGTCAACGCCCAGATTTTAAGCGGCCTAAAACAAGGAGAGACGGTCATTATTGGTGAAAACTCAGGCGCCAAGGACAGTG
GCAAAGGCCAACGTAGTCGCCCACCTGGCATGTAA

Upstream 100 bases:

>100_bases
CCTTTCTTAAACTTAATGCCATGGTCTTTTTTGACCAGTGCGCTATATTAGGTTATTTAAAATTTAGTAATCCTTTTAAA
ATCTGGTTCCGGACTGTGTT

Downstream 100 bases:

>100_bases
TGATCGACTCAGGCGCTTGGTGTTTATCACCGCTGCTGCCCATTATCTGCCATAAAATGCGCCGATAAGCGTAAGCCATA
TTCGGCGCAAAGCGGCAAGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAFISNQKTRKSVIKWTIIALIALALAVLAYNKFKPKQEETNYITATAEMGDVENNVMASGKVKALNTVDVGAQVSGEVT
KLFVDVGDEVKKGDLIAQIDQVTQKNNLSNQQATLEQGRAALQSAQADYYSRKAGLESAQADLASREADLKQAQSDYARL
RPLLAMDAISQQEVENARTTVEKAQASVAAAKAAIKTAQAALITAQSNIASSEADVRKAQTNLDTASTDLGYTTIRAPMD
GTVVAVVTEQGTTVNANQAAPTIVKLADLSTVRINAQISEADVINVKAGMPVYFNTIGDPDKKYDAILTAVEPAPEQISD
TSSTDSAIYYVGYIEVPNPERRFRIDMTAQVYVIIDKATNTLLVPSAAIKKGPNKGASGAARSRSGAGGNNKPASAAPAP
VKGPYVRVLNADGSVENRAVKVGIDNRVNAQILSGLKQGETVIIGENSGAKDSGKGQRSRPPGM

Sequences:

>Translated_464_residues
MAFISNQKTRKSVIKWTIIALIALALAVLAYNKFKPKQEETNYITATAEMGDVENNVMASGKVKALNTVDVGAQVSGEVT
KLFVDVGDEVKKGDLIAQIDQVTQKNNLSNQQATLEQGRAALQSAQADYYSRKAGLESAQADLASREADLKQAQSDYARL
RPLLAMDAISQQEVENARTTVEKAQASVAAAKAAIKTAQAALITAQSNIASSEADVRKAQTNLDTASTDLGYTTIRAPMD
GTVVAVVTEQGTTVNANQAAPTIVKLADLSTVRINAQISEADVINVKAGMPVYFNTIGDPDKKYDAILTAVEPAPEQISD
TSSTDSAIYYVGYIEVPNPERRFRIDMTAQVYVIIDKATNTLLVPSAAIKKGPNKGASGAARSRSGAGGNNKPASAAPAP
VKGPYVRVLNADGSVENRAVKVGIDNRVNAQILSGLKQGETVIIGENSGAKDSGKGQRSRPPGM
>Mature_463_residues
AFISNQKTRKSVIKWTIIALIALALAVLAYNKFKPKQEETNYITATAEMGDVENNVMASGKVKALNTVDVGAQVSGEVTK
LFVDVGDEVKKGDLIAQIDQVTQKNNLSNQQATLEQGRAALQSAQADYYSRKAGLESAQADLASREADLKQAQSDYARLR
PLLAMDAISQQEVENARTTVEKAQASVAAAKAAIKTAQAALITAQSNIASSEADVRKAQTNLDTASTDLGYTTIRAPMDG
TVVAVVTEQGTTVNANQAAPTIVKLADLSTVRINAQISEADVINVKAGMPVYFNTIGDPDKKYDAILTAVEPAPEQISDT
SSTDSAIYYVGYIEVPNPERRFRIDMTAQVYVIIDKATNTLLVPSAAIKKGPNKGASGAARSRSGAGGNNKPASAAPAPV
KGPYVRVLNADGSVENRAVKVGIDNRVNAQILSGLKQGETVIIGENSGAKDSGKGQRSRPPGM

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=439, Percent_Identity=34.3963553530752, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1790519, Length=244, Percent_Identity=28.6885245901639, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787013, Length=314, Percent_Identity=26.4331210191083, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789041, Length=242, Percent_Identity=28.099173553719, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789900, Length=267, Percent_Identity=26.9662921348315, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48996; Mature: 48864

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFISNQKTRKSVIKWTIIALIALALAVLAYNKFKPKQEETNYITATAEMGDVENNVMAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEC
GKVKALNTVDVGAQVSGEVTKLFVDVGDEVKKGDLIAQIDQVTQKNNLSNQQATLEQGRA
CCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ALQSAQADYYSRKAGLESAQADLASREADLKQAQSDYARLRPLLAMDAISQQEVENARTT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEKAQASVAAAKAAIKTAQAALITAQSNIASSEADVRKAQTNLDTASTDLGYTTIRAPMD
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCC
GTVVAVVTEQGTTVNANQAAPTIVKLADLSTVRINAQISEADVINVKAGMPVYFNTIGDP
CEEEEEEECCCCEEECCCCCCEEEEEECCEEEEEEEEECCCEEEEEECCCCEEEECCCCC
DKKYDAILTAVEPAPEQISDTSSTDSAIYYVGYIEVPNPERRFRIDMTAQVYVIIDKATN
CHHHHHEEEECCCCCHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCC
TLLVPSAAIKKGPNKGASGAARSRSGAGGNNKPASAAPAPVKGPYVRVLNADGSVENRAV
EEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
KVGIDNRVNAQILSGLKQGETVIIGENSGAKDSGKGQRSRPPGM
EEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AFISNQKTRKSVIKWTIIALIALALAVLAYNKFKPKQEETNYITATAEMGDVENNVMAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEC
GKVKALNTVDVGAQVSGEVTKLFVDVGDEVKKGDLIAQIDQVTQKNNLSNQQATLEQGRA
CCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ALQSAQADYYSRKAGLESAQADLASREADLKQAQSDYARLRPLLAMDAISQQEVENARTT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEKAQASVAAAKAAIKTAQAALITAQSNIASSEADVRKAQTNLDTASTDLGYTTIRAPMD
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCC
GTVVAVVTEQGTTVNANQAAPTIVKLADLSTVRINAQISEADVINVKAGMPVYFNTIGDP
CEEEEEEECCCCEEECCCCCCEEEEEECCEEEEEEEEECCCEEEEEECCCCEEEECCCCC
DKKYDAILTAVEPAPEQISDTSSTDSAIYYVGYIEVPNPERRFRIDMTAQVYVIIDKATN
CHHHHHEEEECCCCCHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCC
TLLVPSAAIKKGPNKGASGAARSRSGAGGNNKPASAAPAPVKGPYVRVLNADGSVENRAV
EEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
KVGIDNRVNAQILSGLKQGETVIIGENSGAKDSGKGQRSRPPGM
EEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]