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The map label for this gene is dacA [H]

Identifier: 148653555

GI number: 148653555

Start: 2176294

End: 2177535

Strand: Direct

Name: dacA [H]

Synonym: PsycPRwf_1758

Alternate gene names: 148653555

Gene position: 2176294-2177535 (Clockwise)

Preceding gene: 148653554

Following gene: 148653557

Centisome position: 73.06

GC content: 45.97

Gene sequence:

>1242_bases
ATGACCAAATATGGTGTCTTAGATATGACTGCTCCTGTAACCCAGCTGTTACCTATATTAGATAAAGCTCAATCAGTAAC
CCGTTTATCAACGGCTAAATCTCTATTTTTAGGCTCTAGTATGCTGCTGCTATCTAGTGCCTCATTAGCAGCGACGGCTC
CGGTACCCCCACCTGAAATGGACAACACCGCCTATATCTTGATGGACTATGATACGGGAGTCATTTTGGCAGAAAAAAAT
GCCGACCAGCCACTTCCTCCAGCGTCACTTACCAAAATGATGACCAGTCACATCATTGAAAAGAAGCTGATGTCGGGTGA
AATGAAAGAGACTGACCCCGTATTAATGACAGAAAGTGCCTGGTGTCGCGGTACCAGCGATCAGTCTTGTATGTATGTGC
CGGTCAATAAAAGCGCCCCGGCTATTGATATGCTACGCGGTATTATTATCCAGTCAGGTAACGATGCCTCAAAGGCTATG
GCTGAGCACATTGCTGGTAGCGAAAGTGCGTTCGCTCAAATCATGAACGCAGAAGCCAAAGCCTTAGGCATGACCAACAC
CCATTTTGTCAACGCCACCGGTATGCCAGATGAAGGTCACAGAGCTTCTGCACGCGACTTGGCCATATTATCACGCGCTA
TTATCAAGCAAGGTGGTGATTACTATAAGCTTTACTCTGAAAAAGAGTTCACTTATAACAACATCACTCAAGGTAACCGT
AACGCCCTATTGCGTACCGACCCCACTGTTGATGGCCTAAAAACGGGGCATACCGATGCTGCTGGCTACTGTTTAGCCGC
GTCAAGTAAGCGTAATGACATGCGTCTAATTTCTGTCATTATGGGCAGCAAAAGCATGCAGGCACGTGCCGATCAGTCAC
GTGAATTATTAAACTGGGGCTTTGGTCACTTCGACACTAAAGTCGTTGCACCTGCCAATCAGTTTGTAGACTCAGTGCCT
GTTTATTTTGGCAAGCAAGACTCAGTAAAAGCAGCCACTGGTGAAGCATTAAAAGTATTAATCGCTAAGACCCAGACGGA
TAAAATCAGCACTGTGGTAAACATCTCAGATAAAGTAGAAGCCCCCATCAAAAAAGGCCAAGAGATCGGCCGCATGATGG
CAGTAGTAGATGGAAAATCTGTGGCCTCTGTGCCTATCGTTGCCACTGAAGATGTGGAACAAGCTGGTATCTTTAAGCGT
GCTTGGCAGCATGTGGTAAAATGGTTTAGCAACTTATTCTAA

Upstream 100 bases:

>100_bases
TAGCTCGCCTACTGTGCCTCTGCTTTAAAAAATAAGCCACACATCTTGCCTGCGTCTGCTATTGGTTCATTGACAGGCCT
ACTCTACCACTGAATAACCT

Downstream 100 bases:

>100_bases
CGCGAGATAGAGCGTAACGCACAAAAAAGGGGCGGTCTAAGACCGCCCCTTTTTTTATACTTTAAAGCTCAAGGGTGCTA
CTTTTTATGCGAGCCTTTTT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTKYGVLDMTAPVTQLLPILDKAQSVTRLSTAKSLFLGSSMLLLSSASLAATAPVPPPEMDNTAYILMDYDTGVILAEKN
ADQPLPPASLTKMMTSHIIEKKLMSGEMKETDPVLMTESAWCRGTSDQSCMYVPVNKSAPAIDMLRGIIIQSGNDASKAM
AEHIAGSESAFAQIMNAEAKALGMTNTHFVNATGMPDEGHRASARDLAILSRAIIKQGGDYYKLYSEKEFTYNNITQGNR
NALLRTDPTVDGLKTGHTDAAGYCLAASSKRNDMRLISVIMGSKSMQARADQSRELLNWGFGHFDTKVVAPANQFVDSVP
VYFGKQDSVKAATGEALKVLIAKTQTDKISTVVNISDKVEAPIKKGQEIGRMMAVVDGKSVASVPIVATEDVEQAGIFKR
AWQHVVKWFSNLF

Sequences:

>Translated_413_residues
MTKYGVLDMTAPVTQLLPILDKAQSVTRLSTAKSLFLGSSMLLLSSASLAATAPVPPPEMDNTAYILMDYDTGVILAEKN
ADQPLPPASLTKMMTSHIIEKKLMSGEMKETDPVLMTESAWCRGTSDQSCMYVPVNKSAPAIDMLRGIIIQSGNDASKAM
AEHIAGSESAFAQIMNAEAKALGMTNTHFVNATGMPDEGHRASARDLAILSRAIIKQGGDYYKLYSEKEFTYNNITQGNR
NALLRTDPTVDGLKTGHTDAAGYCLAASSKRNDMRLISVIMGSKSMQARADQSRELLNWGFGHFDTKVVAPANQFVDSVP
VYFGKQDSVKAATGEALKVLIAKTQTDKISTVVNISDKVEAPIKKGQEIGRMMAVVDGKSVASVPIVATEDVEQAGIFKR
AWQHVVKWFSNLF
>Mature_412_residues
TKYGVLDMTAPVTQLLPILDKAQSVTRLSTAKSLFLGSSMLLLSSASLAATAPVPPPEMDNTAYILMDYDTGVILAEKNA
DQPLPPASLTKMMTSHIIEKKLMSGEMKETDPVLMTESAWCRGTSDQSCMYVPVNKSAPAIDMLRGIIIQSGNDASKAMA
EHIAGSESAFAQIMNAEAKALGMTNTHFVNATGMPDEGHRASARDLAILSRAIIKQGGDYYKLYSEKEFTYNNITQGNRN
ALLRTDPTVDGLKTGHTDAAGYCLAASSKRNDMRLISVIMGSKSMQARADQSRELLNWGFGHFDTKVVAPANQFVDSVPV
YFGKQDSVKAATGEALKVLIAKTQTDKISTVVNISDKVEAPIKKGQEIGRMMAVVDGKSVASVPIVATEDVEQAGIFKRA
WQHVVKWFSNLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=395, Percent_Identity=40.253164556962, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1786851, Length=362, Percent_Identity=39.5027624309392, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI87082022, Length=381, Percent_Identity=36.745406824147, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI87082054, Length=250, Percent_Identity=29.2, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44682; Mature: 44551

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYGVLDMTAPVTQLLPILDKAQSVTRLSTAKSLFLGSSMLLLSSASLAATAPVPPPEM
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCCCC
DNTAYILMDYDTGVILAEKNADQPLPPASLTKMMTSHIIEKKLMSGEMKETDPVLMTESA
CCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
WCRGTSDQSCMYVPVNKSAPAIDMLRGIIIQSGNDASKAMAEHIAGSESAFAQIMNAEAK
CCCCCCCCCEEEEECCCCCCHHHHHHHHEEECCCCHHHHHHHHHCCCHHHHHHHHCCHHH
ALGMTNTHFVNATGMPDEGHRASARDLAILSRAIIKQGGDYYKLYSEKEFTYNNITQGNR
HCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
NALLRTDPTVDGLKTGHTDAAGYCLAASSKRNDMRLISVIMGSKSMQARADQSRELLNWG
CEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
FGHFDTKVVAPANQFVDSVPVYFGKQDSVKAATGEALKVLIAKTQTDKISTVVNISDKVE
CCCCCCEEECCHHHHHHCCCCEECCCCCCCHHCCHHEEEEEEECCCCCEEEEEECCHHHH
APIKKGQEIGRMMAVVDGKSVASVPIVATEDVEQAGIFKRAWQHVVKWFSNLF
HHHHHHHHHHHHHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKYGVLDMTAPVTQLLPILDKAQSVTRLSTAKSLFLGSSMLLLSSASLAATAPVPPPEM
CCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCCCC
DNTAYILMDYDTGVILAEKNADQPLPPASLTKMMTSHIIEKKLMSGEMKETDPVLMTESA
CCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
WCRGTSDQSCMYVPVNKSAPAIDMLRGIIIQSGNDASKAMAEHIAGSESAFAQIMNAEAK
CCCCCCCCCEEEEECCCCCCHHHHHHHHEEECCCCHHHHHHHHHCCCHHHHHHHHCCHHH
ALGMTNTHFVNATGMPDEGHRASARDLAILSRAIIKQGGDYYKLYSEKEFTYNNITQGNR
HCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
NALLRTDPTVDGLKTGHTDAAGYCLAASSKRNDMRLISVIMGSKSMQARADQSRELLNWG
CEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
FGHFDTKVVAPANQFVDSVPVYFGKQDSVKAATGEALKVLIAKTQTDKISTVVNISDKVE
CCCCCCEEECCHHHHHHCCCCEECCCCCCCHHCCHHEEEEEEECCCCCEEEEEECCHHHH
APIKKGQEIGRMMAVVDGKSVASVPIVATEDVEQAGIFKRAWQHVVKWFSNLF
HHHHHHHHHHHHHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]