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The map label for this gene is metG

Identifier: 148653506

GI number: 148653506

Start: 2113541

End: 2115634

Strand: Direct

Name: metG

Synonym: PsycPRwf_1709

Alternate gene names: 148653506

Gene position: 2113541-2115634 (Clockwise)

Preceding gene: 148653504

Following gene: 148653508

Centisome position: 70.95

GC content: 44.79

Gene sequence:

>2094_bases
GTGCGTCAAATACTCGTAACCAGTGCCCTGCCCTATGCCAATGGCCCTATTCACTTAGGACATTTGGTGGAATACATTCA
AACTGACATTTGGGTACGAGCGATGAAGGCTCAAAGCCATCAAGTCACCTACGTTTGTGCCGATGACGCTCACGGCACCG
CCATTATGCTAAAAGCTGAAGCCAATGGGGTCACGCCAGAGCAGCAAATCGCCACTGTAAAAGCCTCTCACGAAGCAGAT
TTTAGTAAATTCTTAATTGGTTTTGACAACTACCACTCTACTCACTCTGAAGAAAACCGTGAGCTATCTGAGTTAATTTA
TCGCCGTCTAAATGGTGAAGGTCATATCTCAACCAAAGATGTTGAGCAATTATTTGATCCAGAAAAAGGCTTATTTTTAG
CCGACCGTTTCGTGAAAGGCGAATGCCCAGAATGTGGGGCTCAAGACCAATATGGTGATAACTGTGAAGTGTGCGGCACC
ACTTACAATGCCACTGAACTTAAAAACCCGTATTCGACCTTATCGGGTGCAACCCCTGTATTAAGAACCTCAAAACACTA
CTTCTTTAACCTGCCTGAATTTGAGAGCTTCTTAAAACAGTGGACCAAAGATGAGGGACGTTTACAGCCCTCAGTTTCTA
ACAAGCTTCAGGAGTGGTTTGAAGCCGGCCTTGCCAGCTGGGACATCTCACGTGATGCGCCCTATTTCGGTTTCAAAATC
CCAGACACGCCAGAGGGTGAGCCGGATAAATACTTTTATGTGTGGCTAGATGCCCCTGTGGGCTATATGGCCAGTTTTAA
AAACCTATGCAAACAGCGTCAAGGCTCCACTGATCCACAACAACCAGAATTGAGCTTCGATGATTATTGGTTACAAGAAA
ATCAGCATAAAACTGAGCTGTATCACTTCATCGGCAAAGACATTGTTTATTTCCACGCTTTATTTTGGCCCGCCATGCTG
GCCGGCAGTGAATACCGTACCCCAACGGGGGTGTTCGCTCACGGCTTCTTAATGGTCAATGGCGATAAAATGAGCAAATC
TCGCGGCACCTTTATCCAAGCCGAAACCTTTGCCAAACATTTAAACCCTGAGTATCTACGTTACTATTTCGCCAGCAAAC
TGTCTGAGAAAGTTGAAGACATTAATCTAGACTTTGCAGATTTTATGCAAAAGGTGAACTCAGACATGGTCGGTAAAGTG
GTGAATATTGCCAGCCGTAGCGCCGGTTTTATTGCCAAAAAATATGATGGCATGCTCTCAGACAGCTGTGCTGAGCCTGA
GTTATTAAATGAGATTGTGCGCGCTGGTGAAGAGATTGCTACGGCTTATGAAAATCGTGAGTTTAGCCGTGCCATGCGCC
TGATCATGCAGTGCGCAGACAAGGCTAACGAATATATCGACAGCAAAAAGCCTTGGGCAATGGCCAAAGAAGAAGGCCGT
GAACAAGAGGTTCAGGATGTATGTACGGTTGCTATCAATATTTTCCGTCAATTGATGGTGTATTTGGCGCCTGTGTTACC
AGAGCTTACAGACAATGCCAAAGCCTTCTTAAATATTGATGACTTAAGCTTTGCCAGCCGCCATGAGTTATTGCTAGGTC
ATAAAATCAATAAGTTTAAGCCGTTAATGCAGCGTATTGAGCAGGCTCAAATTGATGCTATGGTGGCAGACTCCAAACAA
GACTTGTTGGCAACACCAACTGCTCAGGCCGATAACGTAGCTAAAGCAGCTCAAGCGTCAGAAACTAATGATCAGCAAGA
CAGCGACTACATAGAGTTTGATGACTTTATGAAAGTTGAAATGACGGTGGCTCAAGTACTTGAATGTAATCATGTTGAGG
GTGCAGACAAGCTACTGCAGTTCACATTAGACATCGGCAAAGAGCAGCCCATTAATGTCTTTAGTGGCATTCGTAAATTC
TATGAACCTGAGCAACTGGCCAACAAAAAAGTGATTTGTGTCACTAATCTTGCCCCGCGCAAAATGAAATTTGGGGTGTC
AGAAGGTATGATCTTATCATCAGGTGATCCAAAAACAGGACTGGTGGTCATCACGCTACCTGACGAGTGTAAAGTGGGCG
ATAAATTGGCTTAA

Upstream 100 bases:

>100_bases
CGAGGTAAATTCCCATTTTTATGCCATTTGCGGTATCATAGGCGCACATTTTATATTCTGATAATCCTCTTAAAATCAAC
AAACTTAAATAGGTGATTTT

Downstream 100 bases:

>100_bases
GCCTTTTTAAGATAAGTGCCTCTTTATCTTGACTTCCTCCCCTCGCTAAACCGAGGGGATTCCTACAGCTAGACGGTCAA
GCCCGACCGCAAGGATGTTC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MRQILVTSALPYANGPIHLGHLVEYIQTDIWVRAMKAQSHQVTYVCADDAHGTAIMLKAEANGVTPEQQIATVKASHEAD
FSKFLIGFDNYHSTHSEENRELSELIYRRLNGEGHISTKDVEQLFDPEKGLFLADRFVKGECPECGAQDQYGDNCEVCGT
TYNATELKNPYSTLSGATPVLRTSKHYFFNLPEFESFLKQWTKDEGRLQPSVSNKLQEWFEAGLASWDISRDAPYFGFKI
PDTPEGEPDKYFYVWLDAPVGYMASFKNLCKQRQGSTDPQQPELSFDDYWLQENQHKTELYHFIGKDIVYFHALFWPAML
AGSEYRTPTGVFAHGFLMVNGDKMSKSRGTFIQAETFAKHLNPEYLRYYFASKLSEKVEDINLDFADFMQKVNSDMVGKV
VNIASRSAGFIAKKYDGMLSDSCAEPELLNEIVRAGEEIATAYENREFSRAMRLIMQCADKANEYIDSKKPWAMAKEEGR
EQEVQDVCTVAINIFRQLMVYLAPVLPELTDNAKAFLNIDDLSFASRHELLLGHKINKFKPLMQRIEQAQIDAMVADSKQ
DLLATPTAQADNVAKAAQASETNDQQDSDYIEFDDFMKVEMTVAQVLECNHVEGADKLLQFTLDIGKEQPINVFSGIRKF
YEPEQLANKKVICVTNLAPRKMKFGVSEGMILSSGDPKTGLVVITLPDECKVGDKLA

Sequences:

>Translated_697_residues
MRQILVTSALPYANGPIHLGHLVEYIQTDIWVRAMKAQSHQVTYVCADDAHGTAIMLKAEANGVTPEQQIATVKASHEAD
FSKFLIGFDNYHSTHSEENRELSELIYRRLNGEGHISTKDVEQLFDPEKGLFLADRFVKGECPECGAQDQYGDNCEVCGT
TYNATELKNPYSTLSGATPVLRTSKHYFFNLPEFESFLKQWTKDEGRLQPSVSNKLQEWFEAGLASWDISRDAPYFGFKI
PDTPEGEPDKYFYVWLDAPVGYMASFKNLCKQRQGSTDPQQPELSFDDYWLQENQHKTELYHFIGKDIVYFHALFWPAML
AGSEYRTPTGVFAHGFLMVNGDKMSKSRGTFIQAETFAKHLNPEYLRYYFASKLSEKVEDINLDFADFMQKVNSDMVGKV
VNIASRSAGFIAKKYDGMLSDSCAEPELLNEIVRAGEEIATAYENREFSRAMRLIMQCADKANEYIDSKKPWAMAKEEGR
EQEVQDVCTVAINIFRQLMVYLAPVLPELTDNAKAFLNIDDLSFASRHELLLGHKINKFKPLMQRIEQAQIDAMVADSKQ
DLLATPTAQADNVAKAAQASETNDQQDSDYIEFDDFMKVEMTVAQVLECNHVEGADKLLQFTLDIGKEQPINVFSGIRKF
YEPEQLANKKVICVTNLAPRKMKFGVSEGMILSSGDPKTGLVVITLPDECKVGDKLA
>Mature_697_residues
MRQILVTSALPYANGPIHLGHLVEYIQTDIWVRAMKAQSHQVTYVCADDAHGTAIMLKAEANGVTPEQQIATVKASHEAD
FSKFLIGFDNYHSTHSEENRELSELIYRRLNGEGHISTKDVEQLFDPEKGLFLADRFVKGECPECGAQDQYGDNCEVCGT
TYNATELKNPYSTLSGATPVLRTSKHYFFNLPEFESFLKQWTKDEGRLQPSVSNKLQEWFEAGLASWDISRDAPYFGFKI
PDTPEGEPDKYFYVWLDAPVGYMASFKNLCKQRQGSTDPQQPELSFDDYWLQENQHKTELYHFIGKDIVYFHALFWPAML
AGSEYRTPTGVFAHGFLMVNGDKMSKSRGTFIQAETFAKHLNPEYLRYYFASKLSEKVEDINLDFADFMQKVNSDMVGKV
VNIASRSAGFIAKKYDGMLSDSCAEPELLNEIVRAGEEIATAYENREFSRAMRLIMQCADKANEYIDSKKPWAMAKEEGR
EQEVQDVCTVAINIFRQLMVYLAPVLPELTDNAKAFLNIDDLSFASRHELLLGHKINKFKPLMQRIEQAQIDAMVADSKQ
DLLATPTAQADNVAKAAQASETNDQQDSDYIEFDDFMKVEMTVAQVLECNHVEGADKLLQFTLDIGKEQPINVFSGIRKF
YEPEQLANKKVICVTNLAPRKMKFGVSEGMILSSGDPKTGLVVITLPDECKVGDKLA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=568, Percent_Identity=29.0492957746479, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI24308436, Length=543, Percent_Identity=23.572744014733, Blast_Score=129, Evalue=1e-29,
Organism=Escherichia coli, GI1788432, Length=688, Percent_Identity=54.3604651162791, Blast_Score=788, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=604, Percent_Identity=27.9801324503311, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI212645096, Length=530, Percent_Identity=24.1509433962264, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71992781, Length=357, Percent_Identity=26.0504201680672, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321704, Length=571, Percent_Identity=30.122591943958, Blast_Score=257, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6321610, Length=567, Percent_Identity=23.2804232804233, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655311, Length=569, Percent_Identity=29.701230228471, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI21358483, Length=525, Percent_Identity=23.8095238095238, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PSYWF (A5WG58)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280599.1
- ProteinModelPortal:   A5WG58
- STRING:   A5WG58
- GeneID:   5206834
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1709
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- ProtClustDB:   PRK00133
- BioCyc:   PSP56811:PSYCPRWF_1709-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 78753; Mature: 78753

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 346-346

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQILVTSALPYANGPIHLGHLVEYIQTDIWVRAMKAQSHQVTYVCADDAHGTAIMLKAE
CCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEC
ANGVTPEQQIATVKASHEADFSKFLIGFDNYHSTHSEENRELSELIYRRLNGEGHISTKD
CCCCCCHHHHHHHHCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
VEQLFDPEKGLFLADRFVKGECPECGAQDQYGDNCEVCGTTYNATELKNPYSTLSGATPV
HHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCCCH
LRTSKHYFFNLPEFESFLKQWTKDEGRLQPSVSNKLQEWFEAGLASWDISRDAPYFGFKI
HCCCCCCEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEC
PDTPEGEPDKYFYVWLDAPVGYMASFKNLCKQRQGSTDPQQPELSFDDYWLQENQHKTEL
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHH
YHFIGKDIVYFHALFWPAMLAGSEYRTPTGVFAHGFLMVNGDKMSKSRGTFIQAETFAKH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEHHHHHHH
LNPEYLRYYFASKLSEKVEDINLDFADFMQKVNSDMVGKVVNIASRSAGFIAKKYDGMLS
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHCCCCC
DSCAEPELLNEIVRAGEEIATAYENREFSRAMRLIMQCADKANEYIDSKKPWAMAKEEGR
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCH
EQEVQDVCTVAINIFRQLMVYLAPVLPELTDNAKAFLNIDDLSFASRHELLLGHKINKFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHHHH
PLMQRIEQAQIDAMVADSKQDLLATPTAQADNVAKAAQASETNDQQDSDYIEFDDFMKVE
HHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
MTVAQVLECNHVEGADKLLQFTLDIGKEQPINVFSGIRKFYEPEQLANKKVICVTNLAPR
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHCCCEEEEEECCCCC
KMKFGVSEGMILSSGDPKTGLVVITLPDECKVGDKLA
HHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRQILVTSALPYANGPIHLGHLVEYIQTDIWVRAMKAQSHQVTYVCADDAHGTAIMLKAE
CCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEC
ANGVTPEQQIATVKASHEADFSKFLIGFDNYHSTHSEENRELSELIYRRLNGEGHISTKD
CCCCCCHHHHHHHHCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
VEQLFDPEKGLFLADRFVKGECPECGAQDQYGDNCEVCGTTYNATELKNPYSTLSGATPV
HHHHHCCCCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCCCH
LRTSKHYFFNLPEFESFLKQWTKDEGRLQPSVSNKLQEWFEAGLASWDISRDAPYFGFKI
HCCCCCCEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEC
PDTPEGEPDKYFYVWLDAPVGYMASFKNLCKQRQGSTDPQQPELSFDDYWLQENQHKTEL
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHH
YHFIGKDIVYFHALFWPAMLAGSEYRTPTGVFAHGFLMVNGDKMSKSRGTFIQAETFAKH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEHHHHHHH
LNPEYLRYYFASKLSEKVEDINLDFADFMQKVNSDMVGKVVNIASRSAGFIAKKYDGMLS
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHCCCCC
DSCAEPELLNEIVRAGEEIATAYENREFSRAMRLIMQCADKANEYIDSKKPWAMAKEEGR
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCH
EQEVQDVCTVAINIFRQLMVYLAPVLPELTDNAKAFLNIDDLSFASRHELLLGHKINKFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHHHH
PLMQRIEQAQIDAMVADSKQDLLATPTAQADNVAKAAQASETNDQQDSDYIEFDDFMKVE
HHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
MTVAQVLECNHVEGADKLLQFTLDIGKEQPINVFSGIRKFYEPEQLANKKVICVTNLAPR
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHCCCEEEEEECCCCC
KMKFGVSEGMILSSGDPKTGLVVITLPDECKVGDKLA
HHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA