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The map label for this gene is lmrB [H]

Identifier: 148653269

GI number: 148653269

Start: 1800001

End: 1801476

Strand: Direct

Name: lmrB [H]

Synonym: PsycPRwf_1468

Alternate gene names: 148653269

Gene position: 1800001-1801476 (Clockwise)

Preceding gene: 148653268

Following gene: 148653270

Centisome position: 60.42

GC content: 44.44

Gene sequence:

>1476_bases
ATGTCTACCGCTGCAAATGCACAAAACCAAAAAATGATAAAACCCATCCCAATATTGATTTCCTTTTTGATTGCTGGATT
TATTGGCCTCTTTAGTGAAACCGCATTAAATATGGCACTTGGCAATTTGATGCAGGAGTTCAACGTAGTCTCCTCAACTG
TTCAGTGGATTACCACAGGCTACTTGCTGACCATGGGCATCCTTATTCCGGTTACCGCCCTACTCATACAATGGTTAACA
ACACGGCAATTATTCATCTCATCCGTATTGTTCTCAATCGCTGGTGTCGTTCTGTCAGGATTGGCGTCAACATTCAATGT
GTTATTGCTGGGCCGTGTGATTCAAGCCATCGGCACAGGCTTATTGATTCCTTTAATGTTCCATACCGTGCTCATCATTT
TCCCCGTTCATAAACGAGGGGTTATCATGGGCGTGGTGGGTTTGGTGATGATGTCAGCGCCCGCTGTCGGGCCTGCAACC
TCAGGGCTCATCATTGAAGCATTGAGCTGGAACTGGATTTTGTGGTTAATCCTGCCATTTCTTCTATTTACCTTGGTTTA
TGGGTTTATTTTCGTCCAGAACGTTTCGACCCTAACCAAACCAAAAGTTGATGTGCTTTCTATCTTTTTATCGACCATTG
GTTTTGGCGGAATTGTCTATGGATTTAGTGTTGCAGGCCACCAAGGTTGGGGCAGCCCGATTGTCATAAGCTCTCTAGCT
ATCGGTTTGGCATCATTGATTGTCTTTTCAATCAGACAGTTCAAAATCGACAACCCAATGCTTGATTTACGCACTCTAAA
ATACCCCATGTTCACTTTAGGTTTATTGAGCGTTTCGGCAACCTTCATGATTATTTTATCAACCATGATTTTGCTGCCTT
TATATCTACAGATTGGTCTGGGTCTGACTGCACTAACAGCAGGTCTTATCTTAATGCCAGGTGGCGCCCTAAACGGTATT
TTATCGCCTGTTGCCGGTCGTGTTTATGATAAGTATGGGCCTAAATACCTACTGACACCCGGTTTTATCATCATGCTATT
CATGCTTTGGAACCTATCAAACATGACTATTGATACTTCAAAAGAAATGGTGATTTTATTGCACTCAGGCTTGATGATTG
GCGTGACCTTCGTCATGATGCCACTGCAAACCCATGGCCTAAATGCCCTACCTGCCAAGCTGTACCCAGATGGCACTGCA
CTGATTAACACCTTGCTGCAAGTATCAGGCTCTATTGGCACAGCATTGGCAATCACCATCATGTCGGCGGCTCAAAACAC
CTACTTGCAGGATTTTCCAAACCCATCGGATCCCGCGCTCGTCGGAGCTTCACTAACCGCAGGGGTGCAAACGGCCTTTA
TTTTAGGCATTGTGCTGGCAATTAGTGGGTTGATTATGTCGCTGTTTATGAACAAGCCGACACAGCAAGAACTGATGTAT
GCTGAAAAAACCGTAGACAGAAACTTGGTAAACTAA

Upstream 100 bases:

>100_bases
TAGCTGAACAGATTCCATCGCTTTTTTCTGAAAAACCGGTGTGAAAAATATCTCTCCTCAAACCCATCAACTTAATTCAT
AAAAAAGAAAGAGTGTTGTC

Downstream 100 bases:

>100_bases
AGAGGACAAATAGGAGTTTACCATGACTAAGAAAATTAAGAGATATAGTGAAGAATTTAAAGCAGAAGCGGTCAAAGCAA
TTGAGAACAATGGTGGCAAT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSTAANAQNQKMIKPIPILISFLIAGFIGLFSETALNMALGNLMQEFNVVSSTVQWITTGYLLTMGILIPVTALLIQWLT
TRQLFISSVLFSIAGVVLSGLASTFNVLLLGRVIQAIGTGLLIPLMFHTVLIIFPVHKRGVIMGVVGLVMMSAPAVGPAT
SGLIIEALSWNWILWLILPFLLFTLVYGFIFVQNVSTLTKPKVDVLSIFLSTIGFGGIVYGFSVAGHQGWGSPIVISSLA
IGLASLIVFSIRQFKIDNPMLDLRTLKYPMFTLGLLSVSATFMIILSTMILLPLYLQIGLGLTALTAGLILMPGGALNGI
LSPVAGRVYDKYGPKYLLTPGFIIMLFMLWNLSNMTIDTSKEMVILLHSGLMIGVTFVMMPLQTHGLNALPAKLYPDGTA
LINTLLQVSGSIGTALAITIMSAAQNTYLQDFPNPSDPALVGASLTAGVQTAFILGIVLAISGLIMSLFMNKPTQQELMY
AEKTVDRNLVN

Sequences:

>Translated_491_residues
MSTAANAQNQKMIKPIPILISFLIAGFIGLFSETALNMALGNLMQEFNVVSSTVQWITTGYLLTMGILIPVTALLIQWLT
TRQLFISSVLFSIAGVVLSGLASTFNVLLLGRVIQAIGTGLLIPLMFHTVLIIFPVHKRGVIMGVVGLVMMSAPAVGPAT
SGLIIEALSWNWILWLILPFLLFTLVYGFIFVQNVSTLTKPKVDVLSIFLSTIGFGGIVYGFSVAGHQGWGSPIVISSLA
IGLASLIVFSIRQFKIDNPMLDLRTLKYPMFTLGLLSVSATFMIILSTMILLPLYLQIGLGLTALTAGLILMPGGALNGI
LSPVAGRVYDKYGPKYLLTPGFIIMLFMLWNLSNMTIDTSKEMVILLHSGLMIGVTFVMMPLQTHGLNALPAKLYPDGTA
LINTLLQVSGSIGTALAITIMSAAQNTYLQDFPNPSDPALVGASLTAGVQTAFILGIVLAISGLIMSLFMNKPTQQELMY
AEKTVDRNLVN
>Mature_490_residues
STAANAQNQKMIKPIPILISFLIAGFIGLFSETALNMALGNLMQEFNVVSSTVQWITTGYLLTMGILIPVTALLIQWLTT
RQLFISSVLFSIAGVVLSGLASTFNVLLLGRVIQAIGTGLLIPLMFHTVLIIFPVHKRGVIMGVVGLVMMSAPAVGPATS
GLIIEALSWNWILWLILPFLLFTLVYGFIFVQNVSTLTKPKVDVLSIFLSTIGFGGIVYGFSVAGHQGWGSPIVISSLAI
GLASLIVFSIRQFKIDNPMLDLRTLKYPMFTLGLLSVSATFMIILSTMILLPLYLQIGLGLTALTAGLILMPGGALNGIL
SPVAGRVYDKYGPKYLLTPGFIIMLFMLWNLSNMTIDTSKEMVILLHSGLMIGVTFVMMPLQTHGLNALPAKLYPDGTAL
INTLLQVSGSIGTALAITIMSAAQNTYLQDFPNPSDPALVGASLTAGVQTAFILGIVLAISGLIMSLFMNKPTQQELMYA
EKTVDRNLVN

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=416, Percent_Identity=26.4423076923077, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789042, Length=449, Percent_Identity=27.8396436525612, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081983, Length=317, Percent_Identity=23.9747634069401, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=23.3990147783251, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788392, Length=400, Percent_Identity=25.75, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=251, Percent_Identity=25.8964143426295, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52763; Mature: 52632

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAANAQNQKMIKPIPILISFLIAGFIGLFSETALNMALGNLMQEFNVVSSTVQWITTG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLLTMGILIPVTALLIQWLTTRQLFISSVLFSIAGVVLSGLASTFNVLLLGRVIQAIGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LLIPLMFHTVLIIFPVHKRGVIMGVVGLVMMSAPAVGPATSGLIIEALSWNWILWLILPF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHHHHHHHHHHHH
LLFTLVYGFIFVQNVSTLTKPKVDVLSIFLSTIGFGGIVYGFSVAGHQGWGSPIVISSLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCHHHHHHHH
IGLASLIVFSIRQFKIDNPMLDLRTLKYPMFTLGLLSVSATFMIILSTMILLPLYLQIGL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTALTAGLILMPGGALNGILSPVAGRVYDKYGPKYLLTPGFIIMLFMLWNLSNMTIDTS
CHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCCEEECC
KEMVILLHSGLMIGVTFVMMPLQTHGLNALPAKLYPDGTALINTLLQVSGSIGTALAITI
CCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
MSAAQNTYLQDFPNPSDPALVGASLTAGVQTAFILGIVLAISGLIMSLFMNKPTQQELMY
HHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AEKTVDRNLVN
HHHHHHHCCCC
>Mature Secondary Structure 
STAANAQNQKMIKPIPILISFLIAGFIGLFSETALNMALGNLMQEFNVVSSTVQWITTG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLLTMGILIPVTALLIQWLTTRQLFISSVLFSIAGVVLSGLASTFNVLLLGRVIQAIGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
LLIPLMFHTVLIIFPVHKRGVIMGVVGLVMMSAPAVGPATSGLIIEALSWNWILWLILPF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHHHHHHHHHHHH
LLFTLVYGFIFVQNVSTLTKPKVDVLSIFLSTIGFGGIVYGFSVAGHQGWGSPIVISSLA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCHHHHHHHH
IGLASLIVFSIRQFKIDNPMLDLRTLKYPMFTLGLLSVSATFMIILSTMILLPLYLQIGL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTALTAGLILMPGGALNGILSPVAGRVYDKYGPKYLLTPGFIIMLFMLWNLSNMTIDTS
CHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCCEEECC
KEMVILLHSGLMIGVTFVMMPLQTHGLNALPAKLYPDGTALINTLLQVSGSIGTALAITI
CCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
MSAAQNTYLQDFPNPSDPALVGASLTAGVQTAFILGIVLAISGLIMSLFMNKPTQQELMY
HHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AEKTVDRNLVN
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]