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The map label for this gene is cca [H]

Identifier: 148653075

GI number: 148653075

Start: 1565497

End: 1566738

Strand: Direct

Name: cca [H]

Synonym: PsycPRwf_1271

Alternate gene names: 148653075

Gene position: 1565497-1566738 (Clockwise)

Preceding gene: 148653073

Following gene: 148653076

Centisome position: 52.55

GC content: 45.65

Gene sequence:

>1242_bases
ATGCAAGTTTATATTGTGGGTGGCGCAGTCAGAGACGAGCTACTCAACAGACCCTATACCGATAAAGACTTCGTTGTGGT
AGGCGCCACACCCGAGCAAATGATTGAAGCCGGCTTTATCCAGGTAGGCGCAGATTTTCCGGTATTTTTGCACCCTAAAA
CCAAAGAAGAGTATGCCTTAGCACGTACTGAGCGTAAATCTGGCAAAGGGTATAAAGGCTTTGAGGTGTATGCCTCACCC
GAGGTGACTATTGAAGAGGATTTACTGCGCCGTGATCTGACCATCAATGCCATGGCGATTGAAGTCAAGAGTCTATGGGA
CAGTACGCCTTTAACCGGTGAGGTTATCGACCCCTATGACGGTCAATCAGATATTAAAAACAAAATGCTACGCCATGTAT
CTGAAGCTTTTAGTGAAGACCCTCTGCGGGTATTAAGGATAGCTAGGTTTTATGGACGATTTTATGATCCAAAAGCTGAG
CATAACTTCAGCATTGCACCAGATACCTTAGCGTTAATCGCTACCATCAATGACAGTGATGAACTTGCATGCCTGACTGC
TGAGCGTGTATGGCAAGAAACCAGTCGCGCTTTGATGCAAGACTGTCCATATGCTTACCTAGATTGTTTGCAGTATATGG
GCGCCCTACCCCATATTTTTCCCAGTATTGCCAATGCGTTAGGCGATGACAAGATAAAAGCATTGGTGTTTCAAGCCCTA
CAATTAGCAGGCCAACTGAACTTAAGCTTGCAGCAGCGCTGGGCGATAATCATGTTAAGCTTGGCACAAGAGGTGATAGA
TGTGCTTAATCCACGCCATCACTCAAATCATCATCCGCATCAAATCGATGAGGCCTTATTCAGCGCCTGGGCAAGCAAGC
TTAAACAGGTGAGCCATAACTTGAAGGTACCCAAAAAAACCACTCAGTTTGCTGCATCTTATGCCAATTGCTCAGGGTAT
TTAGCAGCCTATCCATACCTAGGGCAACACAGCGACTCTGATGCCAATTCGTTGGCGAAGCTCATCGTGCTCACTAAAGC
GGATAAAGACCCCTCTGAGCTCCATGAGCTAATACAATTACACAAGGTTATTGAAATGGCGTGGCAGCAGCTTGAGATGA
CTGATTTTATTAAGGCTTATACTCAGATCAGCATGTCGGATATCGACCCAGATTTACAAGGCAGCGCTATTGGTACGGCA
TTGAGTGCCGCTCGAATAAATGCTATTGCTCAGATTTTATAA

Upstream 100 bases:

>100_bases
AGCCTTGAGAATAAAGCGGTTGATTATATCAAAAACATTATTGATACTGATACTTTAATTTATACGCTCTTAATAATAAA
ATTAAAACATAAGGATGACT

Downstream 100 bases:

>100_bases
AAAAGTCACCCAAAATTAGCGTCTTACCCTTGCAAATTTATAATTTTCAGGTAATATAATGCGTCTAATAAAGCTGATTC
AATCGCTTATTATTAGGGCT

Product: tRNA cytidylyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MQVYIVGGAVRDELLNRPYTDKDFVVVGATPEQMIEAGFIQVGADFPVFLHPKTKEEYALARTERKSGKGYKGFEVYASP
EVTIEEDLLRRDLTINAMAIEVKSLWDSTPLTGEVIDPYDGQSDIKNKMLRHVSEAFSEDPLRVLRIARFYGRFYDPKAE
HNFSIAPDTLALIATINDSDELACLTAERVWQETSRALMQDCPYAYLDCLQYMGALPHIFPSIANALGDDKIKALVFQAL
QLAGQLNLSLQQRWAIIMLSLAQEVIDVLNPRHHSNHHPHQIDEALFSAWASKLKQVSHNLKVPKKTTQFAASYANCSGY
LAAYPYLGQHSDSDANSLAKLIVLTKADKDPSELHELIQLHKVIEMAWQQLEMTDFIKAYTQISMSDIDPDLQGSAIGTA
LSAARINAIAQIL

Sequences:

>Translated_413_residues
MQVYIVGGAVRDELLNRPYTDKDFVVVGATPEQMIEAGFIQVGADFPVFLHPKTKEEYALARTERKSGKGYKGFEVYASP
EVTIEEDLLRRDLTINAMAIEVKSLWDSTPLTGEVIDPYDGQSDIKNKMLRHVSEAFSEDPLRVLRIARFYGRFYDPKAE
HNFSIAPDTLALIATINDSDELACLTAERVWQETSRALMQDCPYAYLDCLQYMGALPHIFPSIANALGDDKIKALVFQAL
QLAGQLNLSLQQRWAIIMLSLAQEVIDVLNPRHHSNHHPHQIDEALFSAWASKLKQVSHNLKVPKKTTQFAASYANCSGY
LAAYPYLGQHSDSDANSLAKLIVLTKADKDPSELHELIQLHKVIEMAWQQLEMTDFIKAYTQISMSDIDPDLQGSAIGTA
LSAARINAIAQIL
>Mature_413_residues
MQVYIVGGAVRDELLNRPYTDKDFVVVGATPEQMIEAGFIQVGADFPVFLHPKTKEEYALARTERKSGKGYKGFEVYASP
EVTIEEDLLRRDLTINAMAIEVKSLWDSTPLTGEVIDPYDGQSDIKNKMLRHVSEAFSEDPLRVLRIARFYGRFYDPKAE
HNFSIAPDTLALIATINDSDELACLTAERVWQETSRALMQDCPYAYLDCLQYMGALPHIFPSIANALGDDKIKALVFQAL
QLAGQLNLSLQQRWAIIMLSLAQEVIDVLNPRHHSNHHPHQIDEALFSAWASKLKQVSHNLKVPKKTTQFAASYANCSGY
LAAYPYLGQHSDSDANSLAKLIVLTKADKDPSELHELIQLHKVIEMAWQQLEMTDFIKAYTQISMSDIDPDLQGSAIGTA
LSAARINAIAQIL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=247, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789436, Length=432, Percent_Identity=38.4259259259259, Blast_Score=264, Evalue=9e-72,
Organism=Drosophila melanogaster, GI281360112, Length=204, Percent_Identity=28.921568627451, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21357337, Length=204, Percent_Identity=28.921568627451, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46181; Mature: 46181

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYIVGGAVRDELLNRPYTDKDFVVVGATPEQMIEAGFIQVGADFPVFLHPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHCHHEECCCCCEEECCCCCHHHHH
ARTERKSGKGYKGFEVYASPEVTIEEDLLRRDLTINAMAIEVKSLWDSTPLTGEVIDPYD
HHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCEEHHEEEHHHHHCCCCCCCCCCCCCC
GQSDIKNKMLRHVSEAFSEDPLRVLRIARFYGRFYDPKAEHNFSIAPDTLALIATINDSD
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCC
ELACLTAERVWQETSRALMQDCPYAYLDCLQYMGALPHIFPSIANALGDDKIKALVFQAL
CEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
QLAGQLNLSLQQRWAIIMLSLAQEVIDVLNPRHHSNHHPHQIDEALFSAWASKLKQVSHN
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LKVPKKTTQFAASYANCSGYLAAYPYLGQHSDSDANSLAKLIVLTKADKDPSELHELIQL
CCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHH
HKVIEMAWQQLEMTDFIKAYTQISMSDIDPDLQGSAIGTALSAARINAIAQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MQVYIVGGAVRDELLNRPYTDKDFVVVGATPEQMIEAGFIQVGADFPVFLHPKTKEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHHCHHEECCCCCEEECCCCCHHHHH
ARTERKSGKGYKGFEVYASPEVTIEEDLLRRDLTINAMAIEVKSLWDSTPLTGEVIDPYD
HHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCEEHHEEEHHHHHCCCCCCCCCCCCCC
GQSDIKNKMLRHVSEAFSEDPLRVLRIARFYGRFYDPKAEHNFSIAPDTLALIATINDSD
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCC
ELACLTAERVWQETSRALMQDCPYAYLDCLQYMGALPHIFPSIANALGDDKIKALVFQAL
CEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHH
QLAGQLNLSLQQRWAIIMLSLAQEVIDVLNPRHHSNHHPHQIDEALFSAWASKLKQVSHN
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LKVPKKTTQFAASYANCSGYLAAYPYLGQHSDSDANSLAKLIVLTKADKDPSELHELIQL
CCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHH
HKVIEMAWQQLEMTDFIKAYTQISMSDIDPDLQGSAIGTALSAARINAIAQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA