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The map label for this gene is trmL [H]

Identifier: 148652780

GI number: 148652780

Start: 1159231

End: 1159773

Strand: Direct

Name: trmL [H]

Synonym: PsycPRwf_0973

Alternate gene names: 148652780

Gene position: 1159231-1159773 (Clockwise)

Preceding gene: 148652776

Following gene: 148652785

Centisome position: 38.91

GC content: 48.25

Gene sequence:

>543_bases
ATGACCATTCACATTGTGCTTGTGCACCCAAAAATTCCTAACAATACCGGCAGCGCCATCCGTTTGTGTGCCAACACCGG
AGCTCAGCTGCACTTGGTTGAGCCATTAGGGTTTGATTTAGACGATAAGAAGCTGCGCCGTGCAGGGCTTGATTATCATG
AATACGCAGCCATGCAAGTGCACAAAAACTGGCAAGTGGCGAAAGAGGCGCTACAAGCAGCAGGCTGTCATAAGATGGTG
GCATTGACTACTAAGCTGAGTCATCCGTTTTATGAGTATGATTTTGGCGATAGCAATGCGGCTGAGCATGAGAGTATTGC
TTTGGTATTTGGTTCAGAGACGGCAGGCTTAGCTGATGACATCCGAGCAGACATTGGGGAGAGCAACTGGCTGCGTTTGC
CCATGTTACCAGACTCTCGCAGCCTAAATCTGGCCAATAGTATCAGCATCTGCTTATATGAAGTATGGCGTCAAAAAGGC
TTTTATGGCGATGAAGGGCGAAGCACCGGATATACCGAGCTGACCCCTTATGATCCTAAATAA

Upstream 100 bases:

>100_bases
AGAAATGTGTGAAATTTATCTAAAACTGTCATAGAATTGTAAATTTCGATACACAGACGGACTATTTGTTTTTACTTCGT
CTCATTTAAGGCAACATTTT

Downstream 100 bases:

>100_bases
CCGGCAAGCATTATAAGCAATTGGGCGGCTTGGGTAGCCCTGCGATGCGATTGACATGGCGGGCAACCAAACCGGTATTA
AATAACTGCTGCAGTAATTG

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTIHIVLVHPKIPNNTGSAIRLCANTGAQLHLVEPLGFDLDDKKLRRAGLDYHEYAAMQVHKNWQVAKEALQAAGCHKMV
ALTTKLSHPFYEYDFGDSNAAEHESIALVFGSETAGLADDIRADIGESNWLRLPMLPDSRSLNLANSISICLYEVWRQKG
FYGDEGRSTGYTELTPYDPK

Sequences:

>Translated_180_residues
MTIHIVLVHPKIPNNTGSAIRLCANTGAQLHLVEPLGFDLDDKKLRRAGLDYHEYAAMQVHKNWQVAKEALQAAGCHKMV
ALTTKLSHPFYEYDFGDSNAAEHESIALVFGSETAGLADDIRADIGESNWLRLPMLPDSRSLNLANSISICLYEVWRQKG
FYGDEGRSTGYTELTPYDPK
>Mature_179_residues
TIHIVLVHPKIPNNTGSAIRLCANTGAQLHLVEPLGFDLDDKKLRRAGLDYHEYAAMQVHKNWQVAKEALQAAGCHKMVA
LTTKLSHPFYEYDFGDSNAAEHESIALVFGSETAGLADDIRADIGESNWLRLPMLPDSRSLNLANSISICLYEVWRQKGF
YGDEGRSTGYTELTPYDPK

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=163, Percent_Identity=46.6257668711656, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20048; Mature: 19917

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHIVLVHPKIPNNTGSAIRLCANTGAQLHLVEPLGFDLDDKKLRRAGLDYHEYAAMQV
CEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHE
HKNWQVAKEALQAAGCHKMVALTTKLSHPFYEYDFGDSNAAEHESIALVFGSETAGLADD
CCCHHHHHHHHHHCCCCCEEEEEHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCCCHHH
IRADIGESNWLRLPMLPDSRSLNLANSISICLYEVWRQKGFYGDEGRSTGYTELTPYDPK
HHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIHIVLVHPKIPNNTGSAIRLCANTGAQLHLVEPLGFDLDDKKLRRAGLDYHEYAAMQV
EEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHE
HKNWQVAKEALQAAGCHKMVALTTKLSHPFYEYDFGDSNAAEHESIALVFGSETAGLADD
CCCHHHHHHHHHHCCCCCEEEEEHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCCCHHH
IRADIGESNWLRLPMLPDSRSLNLANSISICLYEVWRQKGFYGDEGRSTGYTELTPYDPK
HHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA