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The map label for this gene is ispH

Identifier: 148652390

GI number: 148652390

Start: 695083

End: 696039

Strand: Direct

Name: ispH

Synonym: PsycPRwf_0578

Alternate gene names: 148652390

Gene position: 695083-696039 (Clockwise)

Preceding gene: 148652383

Following gene: 148652391

Centisome position: 23.33

GC content: 48.28

Gene sequence:

>957_bases
ATGCAAATTTATCTTGCCAACCCTCGTGGGTTTTGTGCCGGTGTTGACCGTGCTATTGCTATTGTAAATGAAGCATTGGC
ACGCTTTGAGCCGCCCATTTATGTACGCCATGAAGTGGTGCATAACAAATTTGTGGTGTCTGATTTGGCCAATCGCGGCG
CGGTGTTCGTAGAAGAATTACATGAAGTACCCGATGGCTCTATCGTTATCTTCTCAGCACATGGGGTGTCAAAAGCGGTA
GAAGATGAGGCTGAGCGCCGAGATTTAACGGTATTTGATGCGACCTGTCCTTTGGTAACCAAAGTGCATATTGAGGTGGC
CAAGTTCGCCCGTGAAAAAATGGATGCGGTGCTTATCGGTCACCAAGGTCACCCAGAAGTTGAGGGCACCATGGGCCGCT
TTAGCGCGCAATATGGTGGCGAGATTCATCTGGTAGAAAATGAGGCAGATGTCGAAAAATTAGAAGTGAGTGATCCTGAT
CGTCTGGCCTTTGTGACTCAGACCACCTTGTCTATGGATGACACAGCAGTGGTCATTGATGCGCTGCGCAAAAAATTCCC
TAACATTCAAGGGCCACGCAAAGATGACATCTGTTATGCCACCCAAAACCGTCAAGATGCAGTCAAAGACTTAGCTCAGC
GCTGCGAAGTGGTATTGGTGGTGGGTTCTCCCAACTCCTCTAACTCAAACCGTCTACGCGAATTAGCGGAGCGTATGAAC
TGCAAAGCTTACTTAATTGATAATGCCGGTGAGATGAAAAAAGAGTGGTTTGCTGGGGTGGGCGCTGTGGGTGTCACCGC
CGGTGCATCAGCGCCAGAGATTCTTATTCAAGAGGTGTTAAATCAGCTTCAACAATGGGGCGGAGATTTACCTGATGAGC
TCAAAGGGATTGAAGAAAATGTGACCTTTAGCTTACCAAAAGAGCTGCGCATCCCCGTAAAAGAGGTGGGCTTATAA

Upstream 100 bases:

>100_bases
TAGTCACAAAATAGTTTGTCTAATGCATAGAAATAAGCCGATGGGCTTAGCTGCGTTTTTCACATTTTATTTCTTCGATT
ATCTATTGTGAAGGTCTGTT

Downstream 100 bases:

>100_bases
GCCCATTGGTTTAATAAATTAAGGATGATTTATGACGTCATATTATCAGTGCATCAGCCGTGAACAGCAACAAGACGGCA
GCTGCACAGCGCATTACCAA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQIYLANPRGFCAGVDRAIAIVNEALARFEPPIYVRHEVVHNKFVVSDLANRGAVFVEELHEVPDGSIVIFSAHGVSKAV
EDEAERRDLTVFDATCPLVTKVHIEVAKFAREKMDAVLIGHQGHPEVEGTMGRFSAQYGGEIHLVENEADVEKLEVSDPD
RLAFVTQTTLSMDDTAVVIDALRKKFPNIQGPRKDDICYATQNRQDAVKDLAQRCEVVLVVGSPNSSNSNRLRELAERMN
CKAYLIDNAGEMKKEWFAGVGAVGVTAGASAPEILIQEVLNQLQQWGGDLPDELKGIEENVTFSLPKELRIPVKEVGL

Sequences:

>Translated_318_residues
MQIYLANPRGFCAGVDRAIAIVNEALARFEPPIYVRHEVVHNKFVVSDLANRGAVFVEELHEVPDGSIVIFSAHGVSKAV
EDEAERRDLTVFDATCPLVTKVHIEVAKFAREKMDAVLIGHQGHPEVEGTMGRFSAQYGGEIHLVENEADVEKLEVSDPD
RLAFVTQTTLSMDDTAVVIDALRKKFPNIQGPRKDDICYATQNRQDAVKDLAQRCEVVLVVGSPNSSNSNRLRELAERMN
CKAYLIDNAGEMKKEWFAGVGAVGVTAGASAPEILIQEVLNQLQQWGGDLPDELKGIEENVTFSLPKELRIPVKEVGL
>Mature_318_residues
MQIYLANPRGFCAGVDRAIAIVNEALARFEPPIYVRHEVVHNKFVVSDLANRGAVFVEELHEVPDGSIVIFSAHGVSKAV
EDEAERRDLTVFDATCPLVTKVHIEVAKFAREKMDAVLIGHQGHPEVEGTMGRFSAQYGGEIHLVENEADVEKLEVSDPD
RLAFVTQTTLSMDDTAVVIDALRKKFPNIQGPRKDDICYATQNRQDAVKDLAQRCEVVLVVGSPNSSNSNRLRELAERMN
CKAYLIDNAGEMKKEWFAGVGAVGVTAGASAPEILIQEVLNQLQQWGGDLPDELKGIEENVTFSLPKELRIPVKEVGL

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=65.1898734177215, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PSYWF (A5WCZ2)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279483.1
- STRING:   A5WCZ2
- GeneID:   5204513
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0578
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   PSP56811:PSYCPRWF_0578-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 35028; Mature: 35028

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYLANPRGFCAGVDRAIAIVNEALARFEPPIYVRHEVVHNKFVVSDLANRGAVFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHH
HEVPDGSIVIFSAHGVSKAVEDEAERRDLTVFDATCPLVTKVHIEVAKFAREKMDAVLIG
HHCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEC
HQGHPEVEGTMGRFSAQYGGEIHLVENEADVEKLEVSDPDRLAFVTQTTLSMDDTAVVID
CCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCHHHHHH
ALRKKFPNIQGPRKDDICYATQNRQDAVKDLAQRCEVVLVVGSPNSSNSNRLRELAERMN
HHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCC
CKAYLIDNAGEMKKEWFAGVGAVGVTAGASAPEILIQEVLNQLQQWGGDLPDELKGIEEN
CEEEEECCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
VTFSLPKELRIPVKEVGL
CEEECCHHHCCCHHHHCC
>Mature Secondary Structure
MQIYLANPRGFCAGVDRAIAIVNEALARFEPPIYVRHEVVHNKFVVSDLANRGAVFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHH
HEVPDGSIVIFSAHGVSKAVEDEAERRDLTVFDATCPLVTKVHIEVAKFAREKMDAVLIG
HHCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEC
HQGHPEVEGTMGRFSAQYGGEIHLVENEADVEKLEVSDPDRLAFVTQTTLSMDDTAVVID
CCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCEEEEEEEECCCCCHHHHHH
ALRKKFPNIQGPRKDDICYATQNRQDAVKDLAQRCEVVLVVGSPNSSNSNRLRELAERMN
HHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCC
CKAYLIDNAGEMKKEWFAGVGAVGVTAGASAPEILIQEVLNQLQQWGGDLPDELKGIEEN
CEEEEECCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
VTFSLPKELRIPVKEVGL
CEEECCHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA