Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 148652285

GI number: 148652285

Start: 561020

End: 563080

Strand: Direct

Name: ligA

Synonym: PsycPRwf_0473

Alternate gene names: 148652285

Gene position: 561020-563080 (Clockwise)

Preceding gene: 148652280

Following gene: 148652286

Centisome position: 18.83

GC content: 49.64

Gene sequence:

>2061_bases
ATGACTCAAAACACAGCAGACACTCATGCCTCAGCACAAGCTCAAGAGGTCATTGCAAAAATGCGTACCTTGATCGCACA
GGTGCGTAAACACAACAATGCTTATTATGTGATGGATGAGCCGACCATCAGTGACAATGAATATGACCAGTTGCGCTTAA
GTCTCATTGAGCTTGAGGAGCGTTATCCTGAGTTAACTCAGCCCGACAGTCCGACGGCTAGCGTGGGCGACAATCCTCTG
CCGTCATTCTCCCAAGTGCAGCATGACATTGCTATGCTGTCACTGGGTAATATTTTTAATTATCAAGACTTACAAGAGTT
TATGCGCCGGGTCAATGACCGTCTAAGTGATGCTGATCGAAATCCTGAGTATGAGATTGAGATGAAATTAGATGGTTTGG
CTGTTTCATTAAAATATCATAACGGACAATTTGTGCGCGGCGTGACTCGCGGCGATGGTCAAATGGGCGAGGACATTACC
CAAAACGTGAAGACCATCAATAACATTCCACTGGTGATAGAAGCGGCGCAAGATATTGATGTGCTTGAGGTGCGGGGCGA
GGTACTGATGCCAAAAGCAGGCTTTAAGCGCTTAAACCGTTTGGCACAAGAAAATGGTGATAAAACCTTTGCCAACCCCC
GTAACGCAGCAGCAGGTAGTCTACGTCAATTAGACCCAGCCGTGGCTGCCAGTCGTCCTTTGGCTTTTTATGCGTATTCG
GTGAACCAAGGCTTGCCTGAAGCGATTACTTTGCAATCGGCGGCTTTGACCTGGCTTAAGCAGTTAGGCTTTACTGTTAG
TGACTTTGAACGGGTAAAAACAGCCCAGCAAGTTCAAGACTACTATGAGTCGATGATACAAAAACGGGCAGATTTAGCCT
TTGAAATTGATGGGATGGTTATTAAGGTTGATGACTTAACGCTACAAAGTCAACTGGGCGCGTTATCACGTGAACCGCGC
TGGGCCACCGCCTATAAGTTCCCTGCTGAAACGGTGATGACCACACTCAATAGTATTGAATGGCAAGTGGGGCGTACCGG
TCAGCTGACTCCAGTGGGCAAGCTTGAGCCAGTGCAAGTGGGCGGTGTGACGGTCAGTAACGTGACCTTGCACAACTTCG
GGGAAATCCAGCGCCTTGATGTGCGTGCAGGTGATACCGTCAGCGTCCACCGAGCGGGCGATGTGATACCTAAAGTGACT
CGGGTATGGCATGATTTGCGCCCTGAGGGTACCTCGGCGGTGACGCTGCCAACCCACTGTCCTGTGTGCGACTCACCTGT
GGTATTGCCAGAGGGTGAAGCCTTGGCACGCTGCACCGGTGAGCTGTTTTGTCCTGCGCAGCAGCAAGAGGCACTAATTC
ACTTTGTATCACGTAAAGCGATGGATATTGATGGGCTCGGTGAGCGCTGGTTAATCAGTTTCTTTGAGCATGGCATCGTC
AAAACTGTGGCCGATATTTATCATTTAAATGAGCATGCCGATGAGCTGGTTACTCTAGAAAAATTGGGCGAGAAGTCCGT
TAGCAATATGTTGCGCGCCATTGAAGAGAGTAAAAAAACCACACTGGCGCGCTTTATCTATGCGCTAGGCATTCGCGGTG
TGGGAGAGACCACAGCACAGAATCTGGCACAATATTTTGGTGATTTGCCTGAATTGATGGGCGCCAGCATTGACGCCTTA
GAGGCCGTACCTGACGTGGGCCATATTACCGCTGAGCTTATTTATAATTTCTTCCGTGCTGAGCATAACATCGAAGTCAT
TAACGCATTGCAGCAAGCTGGCGTGTATTGGGATAAGGTAGAGCAGCAGGCCTTAGACAATCTACCCCTTGAGGGCCAAA
CGTGGGTGATAACAGGTACCTTGGTCGCCAGCGGTATGTCACGCGATGATGCCAAAGCGCATCTGCAAGCTTTGGGTGCA
AAGGTCTCAGGCAGCGTATCGGCCAAAACCTCGGCATTATTGGCCGGTGAAAAGGCAGGCTCTAAGTTGACCAAAGCGCA
GAGTCTGGGAGTGCGTGTGGTGTTAGAGGATGAGTTCTTAAAGATGATTGGGGCGTCATGA

Upstream 100 bases:

>100_bases
TCGGCAGCAAAGATGCAAAATTGGTATCATATGACCACTCAAATATTTGAACCAAATGAAACATTAAGCGCAAAAATGCA
ATTAAAATAAGGCCCGCTCT

Downstream 100 bases:

>100_bases
CACCACACTCATCCATTTAATAAAACAAAATGCCGAAATCAATTTAAAAACTCAATCAAATTAGTAGCACTTTAGGCGCA
TAAAAGCGGTATCATAATGC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MTQNTADTHASAQAQEVIAKMRTLIAQVRKHNNAYYVMDEPTISDNEYDQLRLSLIELEERYPELTQPDSPTASVGDNPL
PSFSQVQHDIAMLSLGNIFNYQDLQEFMRRVNDRLSDADRNPEYEIEMKLDGLAVSLKYHNGQFVRGVTRGDGQMGEDIT
QNVKTINNIPLVIEAAQDIDVLEVRGEVLMPKAGFKRLNRLAQENGDKTFANPRNAAAGSLRQLDPAVAASRPLAFYAYS
VNQGLPEAITLQSAALTWLKQLGFTVSDFERVKTAQQVQDYYESMIQKRADLAFEIDGMVIKVDDLTLQSQLGALSREPR
WATAYKFPAETVMTTLNSIEWQVGRTGQLTPVGKLEPVQVGGVTVSNVTLHNFGEIQRLDVRAGDTVSVHRAGDVIPKVT
RVWHDLRPEGTSAVTLPTHCPVCDSPVVLPEGEALARCTGELFCPAQQQEALIHFVSRKAMDIDGLGERWLISFFEHGIV
KTVADIYHLNEHADELVTLEKLGEKSVSNMLRAIEESKKTTLARFIYALGIRGVGETTAQNLAQYFGDLPELMGASIDAL
EAVPDVGHITAELIYNFFRAEHNIEVINALQQAGVYWDKVEQQALDNLPLEGQTWVITGTLVASGMSRDDAKAHLQALGA
KVSGSVSAKTSALLAGEKAGSKLTKAQSLGVRVVLEDEFLKMIGAS

Sequences:

>Translated_686_residues
MTQNTADTHASAQAQEVIAKMRTLIAQVRKHNNAYYVMDEPTISDNEYDQLRLSLIELEERYPELTQPDSPTASVGDNPL
PSFSQVQHDIAMLSLGNIFNYQDLQEFMRRVNDRLSDADRNPEYEIEMKLDGLAVSLKYHNGQFVRGVTRGDGQMGEDIT
QNVKTINNIPLVIEAAQDIDVLEVRGEVLMPKAGFKRLNRLAQENGDKTFANPRNAAAGSLRQLDPAVAASRPLAFYAYS
VNQGLPEAITLQSAALTWLKQLGFTVSDFERVKTAQQVQDYYESMIQKRADLAFEIDGMVIKVDDLTLQSQLGALSREPR
WATAYKFPAETVMTTLNSIEWQVGRTGQLTPVGKLEPVQVGGVTVSNVTLHNFGEIQRLDVRAGDTVSVHRAGDVIPKVT
RVWHDLRPEGTSAVTLPTHCPVCDSPVVLPEGEALARCTGELFCPAQQQEALIHFVSRKAMDIDGLGERWLISFFEHGIV
KTVADIYHLNEHADELVTLEKLGEKSVSNMLRAIEESKKTTLARFIYALGIRGVGETTAQNLAQYFGDLPELMGASIDAL
EAVPDVGHITAELIYNFFRAEHNIEVINALQQAGVYWDKVEQQALDNLPLEGQTWVITGTLVASGMSRDDAKAHLQALGA
KVSGSVSAKTSALLAGEKAGSKLTKAQSLGVRVVLEDEFLKMIGAS
>Mature_685_residues
TQNTADTHASAQAQEVIAKMRTLIAQVRKHNNAYYVMDEPTISDNEYDQLRLSLIELEERYPELTQPDSPTASVGDNPLP
SFSQVQHDIAMLSLGNIFNYQDLQEFMRRVNDRLSDADRNPEYEIEMKLDGLAVSLKYHNGQFVRGVTRGDGQMGEDITQ
NVKTINNIPLVIEAAQDIDVLEVRGEVLMPKAGFKRLNRLAQENGDKTFANPRNAAAGSLRQLDPAVAASRPLAFYAYSV
NQGLPEAITLQSAALTWLKQLGFTVSDFERVKTAQQVQDYYESMIQKRADLAFEIDGMVIKVDDLTLQSQLGALSREPRW
ATAYKFPAETVMTTLNSIEWQVGRTGQLTPVGKLEPVQVGGVTVSNVTLHNFGEIQRLDVRAGDTVSVHRAGDVIPKVTR
VWHDLRPEGTSAVTLPTHCPVCDSPVVLPEGEALARCTGELFCPAQQQEALIHFVSRKAMDIDGLGERWLISFFEHGIVK
TVADIYHLNEHADELVTLEKLGEKSVSNMLRAIEESKKTTLARFIYALGIRGVGETTAQNLAQYFGDLPELMGASIDALE
AVPDVGHITAELIYNFFRAEHNIEVINALQQAGVYWDKVEQQALDNLPLEGQTWVITGTLVASGMSRDDAKAHLQALGAK
VSGSVSAKTSALLAGEKAGSKLTKAQSLGVRVVLEDEFLKMIGAS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=675, Percent_Identity=51.1111111111111, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=528, Percent_Identity=22.9166666666667, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PSYWF (A5WCN7)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279378.1
- ProteinModelPortal:   A5WCN7
- STRING:   A5WCN7
- GeneID:   5205218
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0473
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK830527
- BioCyc:   PSP56811:PSYCPRWF_0473-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75544; Mature: 75413

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 129-129 BINDING 127-127 BINDING 150-150 BINDING 187-187 BINDING 302-302 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNTADTHASAQAQEVIAKMRTLIAQVRKHNNAYYVMDEPTISDNEYDQLRLSLIELEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
RYPELTQPDSPTASVGDNPLPSFSQVQHDIAMLSLGNIFNYQDLQEFMRRVNDRLSDADR
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
NPEYEIEMKLDGLAVSLKYHNGQFVRGVTRGDGQMGEDITQNVKTINNIPLVIEAAQDID
CCCEEEEEEECCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCC
VLEVRGEVLMPKAGFKRLNRLAQENGDKTFANPRNAAAGSLRQLDPAVAASRPLAFYAYS
EEEECCCEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCHHHHCCCCEEEEEEE
VNQGLPEAITLQSAALTWLKQLGFTVSDFERVKTAQQVQDYYESMIQKRADLAFEIDGMV
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
IKVDDLTLQSQLGALSREPRWATAYKFPAETVMTTLNSIEWQVGRTGQLTPVGKLEPVQV
EEEECCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCEEE
GGVTVSNVTLHNFGEIQRLDVRAGDTVSVHRAGDVIPKVTRVWHDLRPEGTSAVTLPTHC
CCEEEECEEECCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCC
PVCDSPVVLPEGEALARCTGELFCPAQQQEALIHFVSRKAMDIDGLGERWLISFFEHGIV
CCCCCCEECCCCCHHHHHCCHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KTVADIYHLNEHADELVTLEKLGEKSVSNMLRAIEESKKTTLARFIYALGIRGVGETTAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
NLAQYFGDLPELMGASIDALEAVPDVGHITAELIYNFFRAEHNIEVINALQQAGVYWDKV
HHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
EQQALDNLPLEGQTWVITGTLVASGMSRDDAKAHLQALGAKVSGSVSAKTSALLAGEKAG
HHHHHHCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHCCHHCCCCCHHHHHHHCCCCCC
SKLTKAQSLGVRVVLEDEFLKMIGAS
CHHHHHHHCCEEEEEHHHHHHHHCCC
>Mature Secondary Structure 
TQNTADTHASAQAQEVIAKMRTLIAQVRKHNNAYYVMDEPTISDNEYDQLRLSLIELEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
RYPELTQPDSPTASVGDNPLPSFSQVQHDIAMLSLGNIFNYQDLQEFMRRVNDRLSDADR
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
NPEYEIEMKLDGLAVSLKYHNGQFVRGVTRGDGQMGEDITQNVKTINNIPLVIEAAQDID
CCCEEEEEEECCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCC
VLEVRGEVLMPKAGFKRLNRLAQENGDKTFANPRNAAAGSLRQLDPAVAASRPLAFYAYS
EEEECCCEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCHHHHCCCCEEEEEEE
VNQGLPEAITLQSAALTWLKQLGFTVSDFERVKTAQQVQDYYESMIQKRADLAFEIDGMV
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
IKVDDLTLQSQLGALSREPRWATAYKFPAETVMTTLNSIEWQVGRTGQLTPVGKLEPVQV
EEEECCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCEEE
GGVTVSNVTLHNFGEIQRLDVRAGDTVSVHRAGDVIPKVTRVWHDLRPEGTSAVTLPTHC
CCEEEECEEECCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCC
PVCDSPVVLPEGEALARCTGELFCPAQQQEALIHFVSRKAMDIDGLGERWLISFFEHGIV
CCCCCCEECCCCCHHHHHCCHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KTVADIYHLNEHADELVTLEKLGEKSVSNMLRAIEESKKTTLARFIYALGIRGVGETTAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
NLAQYFGDLPELMGASIDALEAVPDVGHITAELIYNFFRAEHNIEVINALQQAGVYWDKV
HHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHH
EQQALDNLPLEGQTWVITGTLVASGMSRDDAKAHLQALGAKVSGSVSAKTSALLAGEKAG
HHHHHHCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHCCHHCCCCCHHHHHHHCCCCCC
SKLTKAQSLGVRVVLEDEFLKMIGAS
CHHHHHHHCCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA