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The map label for this gene is gltD [H]

Identifier: 148652216

GI number: 148652216

Start: 495647

End: 497065

Strand: Direct

Name: gltD [H]

Synonym: PsycPRwf_0404

Alternate gene names: 148652216

Gene position: 495647-497065 (Clockwise)

Preceding gene: 148652215

Following gene: 148652217

Centisome position: 16.64

GC content: 48.63

Gene sequence:

>1419_bases
ATGAAGCGTATTAATAACAATTTTCAGTTTTTGGACGTGCCCCGTCGTGAGCCTGAGAAAAAAGACATCGTCACCCGCTC
TACCGAATTTGTGGAGATTTATAGTCCCTTTGAGGCGACTGAGGCGGCGCAGCAAGCCCACCGCTGCTTAGAGTGCGGTA
ACCCTTATTGTGAGTGGAAGTGTCCGGTACATAACTACATTCCTAACTGGTTAAAGCTTGCCACTGAAGGTCAGATTTTC
CAAGCGGCTGAGCTTTGTCACCGCACCAACACTTTACCTGAAGTGTGTGGCCGTGTGTGCCCCCAAGATAGATTATGTGA
AGGCGCTTGTACTTTAAATGATGGCTTTGGCGCTGTGACCATTGGTAATGTCGAAAAATACATCAACGATACGGCCTTCG
CCCTAGGTTGGCGCCCAGATATGAGTGATGTGCAGTGGACGGATAAAAAGGTGGCCATTATTGGTGCCGGTCCTGCTGGT
CTAGGCTGTGCCGATATTTTGGTGCGCGCGGGTGTGAAGCCTGTTGTGTTTGATAAACGCCCTGAAATTGGCGGTTTATT
AACCTTTGGTATTCCTGAATTTAAGATGGAAAAACAGGTGATGCGCAATCGCCGCACAGTATTTGAAGGCATGGGTATTG
AGTTTCGCTTAAATACCGAAATTGGTACAGATGTGACCATTGATGAGCTGTTACAAGACTACGATGCCGTGTTCATGGGC
ATGGGCACGTATACTTATATGCGAGGCGGTTATCCAGGCGAAGAGCTATTTGGGGTGTATGATGCCCTAGATTACTTAAT
TGCCAACGTCAATCGCTGCAATGAGTGGGAACAAGATCCAAGCCAGTACATCAACCTAAAAGACAAAAAGGTTGTGGTGT
TAGGCGGCGGTGATACCGCCATGGATTGTAACCGTACTGCCATCCGTCAAGGTGCTGAGCGTGTGGTTTGTGCCTATCGC
CGAGATGAAGCCAACATGCCAGGCTCGCTACGTGAAGTAAAAAATGCCAAAGAAGAGGGGGTTGAGTTTTTATTTAACCG
TCAGCCGACTCAGATTGTGGGGCTAAATGGTCAAGTGACCGGTGTGAAAGTAGTGACCACTCAGCTGGGCAAACCAGATA
ACCGAGGTCGTCAGCGTCCTGAGCCCATTCCCAATTCAGAAGAGATTATTGAATGTGATGCGGTTATTATGGCGTTTGGC
TTCCGCCCAAGTCCTGCTGACTGGTTTGAGCAAAGCGCAGTTAAGATTGATAACTCAGGGCGCGTTTTGGCGCCTGAAGA
GCAAGCCTTTAAGTTTCAGACCAGCAACCCTAAGATTTTTGCTGGCGGTGATATGGTGCGAGGCTCGGACCTAGTGGTCA
CTGCCATTTGGGAAGGTCGTGAAGCGGCCAAAGGCATCTTGGATTATCTAGAAGTGTAA

Upstream 100 bases:

>100_bases
CTCGTGCGGCAGGTTTTGCGTTTAGTAGATGATTAACAGCAGCCTTTATTTGGCCATAATTGTCCTAAATGTAATACCAA
CTTAAGGAGACAAAACCCTA

Downstream 100 bases:

>100_bases
TCCAGCAAAGGCGCTAAGCTCACTCTTAAGCTGTTTATCAATAAAGCCCTACTTGTTAGGGCTTTATGCTGTCTGGCGAT
TATGAGCTAACTTTAACGCT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKRINNNFQFLDVPRREPEKKDIVTRSTEFVEIYSPFEATEAAQQAHRCLECGNPYCEWKCPVHNYIPNWLKLATEGQIF
QAAELCHRTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNVEKYINDTAFALGWRPDMSDVQWTDKKVAIIGAGPAG
LGCADILVRAGVKPVVFDKRPEIGGLLTFGIPEFKMEKQVMRNRRTVFEGMGIEFRLNTEIGTDVTIDELLQDYDAVFMG
MGTYTYMRGGYPGEELFGVYDALDYLIANVNRCNEWEQDPSQYINLKDKKVVVLGGGDTAMDCNRTAIRQGAERVVCAYR
RDEANMPGSLREVKNAKEEGVEFLFNRQPTQIVGLNGQVTGVKVVTTQLGKPDNRGRQRPEPIPNSEEIIECDAVIMAFG
FRPSPADWFEQSAVKIDNSGRVLAPEEQAFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLEV

Sequences:

>Translated_472_residues
MKRINNNFQFLDVPRREPEKKDIVTRSTEFVEIYSPFEATEAAQQAHRCLECGNPYCEWKCPVHNYIPNWLKLATEGQIF
QAAELCHRTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNVEKYINDTAFALGWRPDMSDVQWTDKKVAIIGAGPAG
LGCADILVRAGVKPVVFDKRPEIGGLLTFGIPEFKMEKQVMRNRRTVFEGMGIEFRLNTEIGTDVTIDELLQDYDAVFMG
MGTYTYMRGGYPGEELFGVYDALDYLIANVNRCNEWEQDPSQYINLKDKKVVVLGGGDTAMDCNRTAIRQGAERVVCAYR
RDEANMPGSLREVKNAKEEGVEFLFNRQPTQIVGLNGQVTGVKVVTTQLGKPDNRGRQRPEPIPNSEEIIECDAVIMAFG
FRPSPADWFEQSAVKIDNSGRVLAPEEQAFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLEV
>Mature_472_residues
MKRINNNFQFLDVPRREPEKKDIVTRSTEFVEIYSPFEATEAAQQAHRCLECGNPYCEWKCPVHNYIPNWLKLATEGQIF
QAAELCHRTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNVEKYINDTAFALGWRPDMSDVQWTDKKVAIIGAGPAG
LGCADILVRAGVKPVVFDKRPEIGGLLTFGIPEFKMEKQVMRNRRTVFEGMGIEFRLNTEIGTDVTIDELLQDYDAVFMG
MGTYTYMRGGYPGEELFGVYDALDYLIANVNRCNEWEQDPSQYINLKDKKVVVLGGGDTAMDCNRTAIRQGAERVVCAYR
RDEANMPGSLREVKNAKEEGVEFLFNRQPTQIVGLNGQVTGVKVVTTQLGKPDNRGRQRPEPIPNSEEIIECDAVIMAFG
FRPSPADWFEQSAVKIDNSGRVLAPEEQAFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=452, Percent_Identity=27.8761061946903, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1789606, Length=469, Percent_Identity=66.7377398720682, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=58.351409978308, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI87082180, Length=463, Percent_Identity=51.6198704103672, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1788468, Length=446, Percent_Identity=28.6995515695067, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789243, Length=443, Percent_Identity=28.6681715575621, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=35.4251012145749, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71984108, Length=458, Percent_Identity=29.2576419213974, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17543860, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=488, Percent_Identity=32.5819672131148, Blast_Score=226, Evalue=7e-60,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=34.9173553719008, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=34.9173553719008, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=34.9173553719008, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=34.9173553719008, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24640763, Length=465, Percent_Identity=26.2365591397849, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18858217, Length=465, Percent_Identity=26.2365591397849, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52761; Mature: 52761

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRINNNFQFLDVPRREPEKKDIVTRSTEFVEIYSPFEATEAAQQAHRCLECGNPYCEWK
CCCCCCCCEEEECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEE
CPVHNYIPNWLKLATEGQIFQAAELCHRTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVT
CCHHHHCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEE
IGNVEKYINDTAFALGWRPDMSDVQWTDKKVAIIGAGPAGLGCADILVRAGVKPVVFDKR
ECCHHHHHCCCEEEEECCCCCCCCEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEECCC
PEIGGLLTFGIPEFKMEKQVMRNRRTVFEGMGIEFRLNTEIGTDVTIDELLQDYDAVFMG
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHC
MGTYTYMRGGYPGEELFGVYDALDYLIANVNRCNEWEQDPSQYINLKDKKVVVLGGGDTA
CCHHEEECCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHCCCCCCEEEEECCCCCC
MDCNRTAIRQGAERVVCAYRRDEANMPGSLREVKNAKEEGVEFLFNRQPTQIVGLNGQVT
CCCCHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
GVKVVTTQLGKPDNRGRQRPEPIPNSEEIIECDAVIMAFGFRPSPADWFEQSAVKIDNSG
EEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCEEEEEECCCCCCHHHHHCCCEEECCCC
RVLAPEEQAFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLEV
CEECCHHHCEEEECCCCEEEECCCEECCCCEEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MKRINNNFQFLDVPRREPEKKDIVTRSTEFVEIYSPFEATEAAQQAHRCLECGNPYCEWK
CCCCCCCCEEEECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEE
CPVHNYIPNWLKLATEGQIFQAAELCHRTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVT
CCHHHHCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEEE
IGNVEKYINDTAFALGWRPDMSDVQWTDKKVAIIGAGPAGLGCADILVRAGVKPVVFDKR
ECCHHHHHCCCEEEEECCCCCCCCEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEECCC
PEIGGLLTFGIPEFKMEKQVMRNRRTVFEGMGIEFRLNTEIGTDVTIDELLQDYDAVFMG
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHC
MGTYTYMRGGYPGEELFGVYDALDYLIANVNRCNEWEQDPSQYINLKDKKVVVLGGGDTA
CCHHEEECCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHCCCCCCEEEEECCCCCC
MDCNRTAIRQGAERVVCAYRRDEANMPGSLREVKNAKEEGVEFLFNRQPTQIVGLNGQVT
CCCCHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEE
GVKVVTTQLGKPDNRGRQRPEPIPNSEEIIECDAVIMAFGFRPSPADWFEQSAVKIDNSG
EEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCEEEEEECCCCCCHHHHHCCCEEECCCC
RVLAPEEQAFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAKGILDYLEV
CEECCHHHCEEEECCCCEEEECCCEECCCCEEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]