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The map label for this gene is mscL

Identifier: 148652193

GI number: 148652193

Start: 457404

End: 457835

Strand: Direct

Name: mscL

Synonym: PsycPRwf_0381

Alternate gene names: 148652193

Gene position: 457404-457835 (Clockwise)

Preceding gene: 148652192

Following gene: 148652199

Centisome position: 15.35

GC content: 41.44

Gene sequence:

>432_bases
ATGAGTATGATGTCAGAGTTTAAAGAGTTTGCCCTAAAGGGTAATGTCATGGATTTGGCAGTGGGTGTGATCATTGGAGG
TGCTTTTTCAGGCATCACAAATTCACTGGTTGAAGACATCATCATGCCGATCGTTGCATTTATCGCCGGTGGTGAACTAA
ACTTCAAGAACATGTTTATCTTATTAGGTGATGCGCCTGAAGGCGTGGCCATGACTTATGATGCGCTAAAAGAAGCCGGT
GTTCCTTTATTGGCCTACGGTAGCTTCATCACAGTATTGATTAACTTCTTAATCTTAGCCTTCATTATCTTCATGATGGT
TAAAGGCATGAACAAAATGCGTCGTAAGAATGAAGTGGAAGAGGTGGTTGAAGAGACCCCATCAGAAGAAGTGTTATTGC
TACGTGAAATTAGCCAGAAACTAAGCAAATAG

Upstream 100 bases:

>100_bases
ATCCCTATTTAAGTACTGCTTTTAAGCGCTAAGCACGTTAAGTGTGCTTACTATATTAGTCGGTGCTATTTTGTTTGCTT
TATATTTTTGGAGAAAATTT

Downstream 100 bases:

>100_bases
TACGCTTATGGCTATCGTAACTTGATACAAGTTAGATGCCACATAAAAAGAGACTAACCTAGGTTGGTCTCTTTTTTTAA
TGTTAGGTGACCGTAAGCTA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSMMSEFKEFALKGNVMDLAVGVIIGGAFSGITNSLVEDIIMPIVAFIAGGELNFKNMFILLGDAPEGVAMTYDALKEAG
VPLLAYGSFITVLINFLILAFIIFMMVKGMNKMRRKNEVEEVVEETPSEEVLLLREISQKLSK

Sequences:

>Translated_143_residues
MSMMSEFKEFALKGNVMDLAVGVIIGGAFSGITNSLVEDIIMPIVAFIAGGELNFKNMFILLGDAPEGVAMTYDALKEAG
VPLLAYGSFITVLINFLILAFIIFMMVKGMNKMRRKNEVEEVVEETPSEEVLLLREISQKLSK
>Mature_142_residues
SMMSEFKEFALKGNVMDLAVGVIIGGAFSGITNSLVEDIIMPIVAFIAGGELNFKNMFILLGDAPEGVAMTYDALKEAGV
PLLAYGSFITVLINFLILAFIIFMMVKGMNKMRRKNEVEEVVEETPSEEVLLLREISQKLSK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=145, Percent_Identity=44.1379310344828, Blast_Score=106, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PSYWF (A5WCE5)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279286.1
- ProteinModelPortal:   A5WCE5
- SMR:   A5WCE5
- STRING:   A5WCE5
- GeneID:   5204905
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0381
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK13952
- BioCyc:   PSP56811:PSYCPRWF_0381-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15716; Mature: 15585

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11b95c98)-; HASH(0x12e9aa20)-; HASH(0x12d4d85c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.7 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMMSEFKEFALKGNVMDLAVGVIIGGAFSGITNSLVEDIIMPIVAFIAGGELNFKNMFI
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LLGDAPEGVAMTYDALKEAGVPLLAYGSFITVLINFLILAFIIFMMVKGMNKMRRKNEVE
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVEETPSEEVLLLREISQKLSK
HHHHHCCCHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SMMSEFKEFALKGNVMDLAVGVIIGGAFSGITNSLVEDIIMPIVAFIAGGELNFKNMFI
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LLGDAPEGVAMTYDALKEAGVPLLAYGSFITVLINFLILAFIIFMMVKGMNKMRRKNEVE
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVEETPSEEVLLLREISQKLSK
HHHHHCCCHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA