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The map label for this gene is polB [H]

Identifier: 148643331

GI number: 148643331

Start: 1274373

End: 1276088

Strand: Reverse

Name: polB [H]

Synonym: Msm_1271

Alternate gene names: 148643331

Gene position: 1276088-1274373 (Counterclockwise)

Preceding gene: 148643336

Following gene: 148643330

Centisome position: 68.86

GC content: 30.19

Gene sequence:

>1716_bases
ATGACTAATAAAATTTTATTAAAATTTGCAAAAAAGGGAATTAACCTATCACCAGAGGCTTATAAAAAAGTTCTTGAAGC
TGAAAATCCTCTAGATTTTACCTCTTCATTAATTGTTAAATTAAAAGGAGGTGATTTTAAATCTAAAGATTTAGTTTCAG
TTAGCGGTCAAACTGTTGATGAAATAATGAGAAATAGCACAATTGAAAAATCAACAACAAGACCTCAATTAACACAAAAA
GAAACTAAAAAACCTACTGTAGAACCTAAACCTAAAGAAGAAACTAAAAAACCTACTGTAGAACCTAAACCTAAAGAAGA
AACTAAAAAACCTACTGTAGAACCTAAACCTAAAGAAGAAACTAAAAAACCATCCAGCATTAGTGATTTTAAAAAAGAAT
CTGATGTTAAAGAAAAATACGTTAACAAGGAAAATGCAGAAGCTTTAGAAACTGTAGAAAATAAAAAAGTCAAATTTAAA
AGGAATTTAACAAAAACAAATGTAGAATATGACTTTAAAATCATACAGGACACAAGTAAAAAATCATACACAAGTGGAGA
ACTTGAAAACTTAATTGCTTATTTCCAAAGCAGATATGAAAAATTAGCTAATATTCTGGTTAAAAGACCGGAACTTAAAA
ATTACACAAAAGTAGCTGATATTGAAGAAGGTCAAAGTAATTTAAGCCTTATTTTAATGGTTAGGGAAATCAGATCCACT
AAAAATGGCCATAAACTTATTGAATTTGAAGATGATACAGGAACTATTCCAATACTATTTTCAAATAAAAATGAAGAAGT
CTTTAAAGAAGCTGAAAAACTAGTTAAAGATGAAGTTATCGGTGTTCTTGCTGATAAAAATGGTGATTTGGCTATCGGAA
ATGAAATTATAAATCCTGGAGTTATGAGAATACCTAAAAAAGAAATGGATTTCAGTATTGTATTCTTATCTGATGTTCAC
ATAGGAAGTTTAACCTTTTTGGAAGATGCATTTACTAGATTTATAGACTGGATTAATTGTGAATTTGGAAATGAAGAACA
AGTAAAAATAGCTGAAAGTGTTAAATATCTTGTTATTGGTGGAGATATTGTAGATGGTATTGGTGTATATCCAAATCAGG
AAAAAGAACTGGCTATTAAAGACATTACTCAGCAATACAATGAAGCAGCCAGATTTTTAGGAAACATTAGAAGTGATATT
AAAATTATTATTGCTCCTGGAAACCACGATGCATCTAGAGTGGCAGAACCTCAACCTGCAGTACCTGAAGAGTATGCAAA
AGCTTTATATGAACTTGATAATGTAGAATTTATAAGTAACCCTGGTGTTGTCTCTTTAGACGGTATTAATGTTTTAATTT
ACCACGGACGTAGTTTTGACGATTTGGTAATGGCAGTTAAAGAGTTTACCCATGAGAAAAACGATTTGTTAATGGAAGAA
TTACTTAAAAAAAGACATTTAGCTCCAATTTACGGAGAAAGAACTCCGCTTGCTTCAGAACTTGAAGATTATTTAGTAAT
TGATGAAGTTCCAGATGTCTTCCATACAGGGCATGTTCACATTAACACCCATAAAAAATACAATGGTATTCATTTAATTA
ATTCTGGAACATTTCAGACTCAGACAGAGTTCCAGAAAATTTATAATATTGAACCAACCCCTGCTGAAGTCCCTGTACTT
TACAAAGGGAAATATAAATGTTTAAAGTTCATTTAA

Upstream 100 bases:

>100_bases
TAATATTTAATCAATACAAAAAAAATTTTTAATGCAAATAAAAATTCAAAATCCAAATTCTCAAAGTTTATTAATATAGA
AAATAAAAATTTTAATTAAT

Downstream 100 bases:

>100_bases
AAATTTTTATAAATAAGAACAAGTTAAAATAATATTAAGTTTAGGTGAAAAAATGGAAAAAAATATTGAAGAAATTATTA
ATGTTTCAGATTATCTTTAT

Product: DNA polymerase II small subunit

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTNKILLKFAKKGINLSPEAYKKVLEAENPLDFTSSLIVKLKGGDFKSKDLVSVSGQTVDEIMRNSTIEKSTTRPQLTQK
ETKKPTVEPKPKEETKKPTVEPKPKEETKKPTVEPKPKEETKKPSSISDFKKESDVKEKYVNKENAEALETVENKKVKFK
RNLTKTNVEYDFKIIQDTSKKSYTSGELENLIAYFQSRYEKLANILVKRPELKNYTKVADIEEGQSNLSLILMVREIRST
KNGHKLIEFEDDTGTIPILFSNKNEEVFKEAEKLVKDEVIGVLADKNGDLAIGNEIINPGVMRIPKKEMDFSIVFLSDVH
IGSLTFLEDAFTRFIDWINCEFGNEEQVKIAESVKYLVIGGDIVDGIGVYPNQEKELAIKDITQQYNEAARFLGNIRSDI
KIIIAPGNHDASRVAEPQPAVPEEYAKALYELDNVEFISNPGVVSLDGINVLIYHGRSFDDLVMAVKEFTHEKNDLLMEE
LLKKRHLAPIYGERTPLASELEDYLVIDEVPDVFHTGHVHINTHKKYNGIHLINSGTFQTQTEFQKIYNIEPTPAEVPVL
YKGKYKCLKFI

Sequences:

>Translated_571_residues
MTNKILLKFAKKGINLSPEAYKKVLEAENPLDFTSSLIVKLKGGDFKSKDLVSVSGQTVDEIMRNSTIEKSTTRPQLTQK
ETKKPTVEPKPKEETKKPTVEPKPKEETKKPTVEPKPKEETKKPSSISDFKKESDVKEKYVNKENAEALETVENKKVKFK
RNLTKTNVEYDFKIIQDTSKKSYTSGELENLIAYFQSRYEKLANILVKRPELKNYTKVADIEEGQSNLSLILMVREIRST
KNGHKLIEFEDDTGTIPILFSNKNEEVFKEAEKLVKDEVIGVLADKNGDLAIGNEIINPGVMRIPKKEMDFSIVFLSDVH
IGSLTFLEDAFTRFIDWINCEFGNEEQVKIAESVKYLVIGGDIVDGIGVYPNQEKELAIKDITQQYNEAARFLGNIRSDI
KIIIAPGNHDASRVAEPQPAVPEEYAKALYELDNVEFISNPGVVSLDGINVLIYHGRSFDDLVMAVKEFTHEKNDLLMEE
LLKKRHLAPIYGERTPLASELEDYLVIDEVPDVFHTGHVHINTHKKYNGIHLINSGTFQTQTEFQKIYNIEPTPAEVPVL
YKGKYKCLKFI
>Mature_570_residues
TNKILLKFAKKGINLSPEAYKKVLEAENPLDFTSSLIVKLKGGDFKSKDLVSVSGQTVDEIMRNSTIEKSTTRPQLTQKE
TKKPTVEPKPKEETKKPTVEPKPKEETKKPTVEPKPKEETKKPSSISDFKKESDVKEKYVNKENAEALETVENKKVKFKR
NLTKTNVEYDFKIIQDTSKKSYTSGELENLIAYFQSRYEKLANILVKRPELKNYTKVADIEEGQSNLSLILMVREIRSTK
NGHKLIEFEDDTGTIPILFSNKNEEVFKEAEKLVKDEVIGVLADKNGDLAIGNEIINPGVMRIPKKEMDFSIVFLSDVHI
GSLTFLEDAFTRFIDWINCEFGNEEQVKIAESVKYLVIGGDIVDGIGVYPNQEKELAIKDITQQYNEAARFLGNIRSDIK
IIIAPGNHDASRVAEPQPAVPEEYAKALYELDNVEFISNPGVVSLDGINVLIYHGRSFDDLVMAVKEFTHEKNDLLMEEL
LKKRHLAPIYGERTPLASELEDYLVIDEVPDVFHTGHVHINTHKKYNGIHLINSGTFQTQTEFQKIYNIEPTPAEVPVLY
KGKYKCLKFI

Specific function: Possesses two activities:a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase [H]

COG id: COG1311

COG function: function code L; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase delta/II small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR012340
- InterPro:   IPR004365
- InterPro:   IPR011149 [H]

Pfam domain/function: PF00149 Metallophos; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 64876; Mature: 64745

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKILLKFAKKGINLSPEAYKKVLEAENPLDFTSSLIVKLKGGDFKSKDLVSVSGQTVD
CCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHH
EIMRNSTIEKSTTRPQLTQKETKKPTVEPKPKEETKKPTVEPKPKEETKKPTVEPKPKEE
HHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCHH
TKKPSSISDFKKESDVKEKYVNKENAEALETVENKKVKFKRNLTKTNVEYDFKIIQDTSK
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCC
KSYTSGELENLIAYFQSRYEKLANILVKRPELKNYTKVADIEEGQSNLSLILMVREIRST
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCC
KNGHKLIEFEDDTGTIPILFSNKNEEVFKEAEKLVKDEVIGVLADKNGDLAIGNEIINPG
CCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCEEECHHHCCCC
VMRIPKKEMDFSIVFLSDVHIGSLTFLEDAFTRFIDWINCEFGNEEQVKIAESVKYLVIG
CEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHCCCEEEEEEC
GDIVDGIGVYPNQEKELAIKDITQQYNEAARFLGNIRSDIKIIIAPGNHDASRVAEPQPA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCCC
VPEEYAKALYELDNVEFISNPGVVSLDGINVLIYHGRSFDDLVMAVKEFTHEKNDLLMEE
CHHHHHHHHHHCCCEEEECCCCEEEECCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
LLKKRHLAPIYGERTPLASELEDYLVIDEVPDVFHTGHVHINTHKKYNGIHLINSGTFQT
HHHHHCCCCCCCCCCCCHHHHHHEEEECCCCHHHHCCEEEEECCCCCCCEEEEECCCCCH
QTEFQKIYNIEPTPAEVPVLYKGKYKCLKFI
HHHHHHHHCCCCCCCCCCEEECCCCHHEECC
>Mature Secondary Structure 
TNKILLKFAKKGINLSPEAYKKVLEAENPLDFTSSLIVKLKGGDFKSKDLVSVSGQTVD
CCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHH
EIMRNSTIEKSTTRPQLTQKETKKPTVEPKPKEETKKPTVEPKPKEETKKPTVEPKPKEE
HHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHCCCCCCCCCCCHH
TKKPSSISDFKKESDVKEKYVNKENAEALETVENKKVKFKRNLTKTNVEYDFKIIQDTSK
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECCCC
KSYTSGELENLIAYFQSRYEKLANILVKRPELKNYTKVADIEEGQSNLSLILMVREIRST
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCC
KNGHKLIEFEDDTGTIPILFSNKNEEVFKEAEKLVKDEVIGVLADKNGDLAIGNEIINPG
CCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCEEECHHHCCCC
VMRIPKKEMDFSIVFLSDVHIGSLTFLEDAFTRFIDWINCEFGNEEQVKIAESVKYLVIG
CEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHCCCEEEEEEC
GDIVDGIGVYPNQEKELAIKDITQQYNEAARFLGNIRSDIKIIIAPGNHDASRVAEPQPA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCCC
VPEEYAKALYELDNVEFISNPGVVSLDGINVLIYHGRSFDDLVMAVKEFTHEKNDLLMEE
CHHHHHHHHHHCCCEEEECCCCEEEECCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHH
LLKKRHLAPIYGERTPLASELEDYLVIDEVPDVFHTGHVHINTHKKYNGIHLINSGTFQT
HHHHHCCCCCCCCCCCCHHHHHHEEEECCCCHHHHCCEEEEECCCCCCCEEEEECCCCCH
QTEFQKIYNIEPTPAEVPVLYKGKYKCLKFI
HHHHHHHHCCCCCCCCCCEEECCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]