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The map label for this gene is frhA [H]

Identifier: 148643184

GI number: 148643184

Start: 1103717

End: 1104934

Strand: Reverse

Name: frhA [H]

Synonym: Msm_1124

Alternate gene names: 148643184

Gene position: 1104934-1103717 (Counterclockwise)

Preceding gene: 148643189

Following gene: 148643183

Centisome position: 59.62

GC content: 36.29

Gene sequence:

>1218_bases
TTGAAAGACAGAATAGTTATATCCCCAACATCTCGTCAAGAGGGCCATGCCGAACTTGTCATGGAAGTCGATGATGAAGG
GATTGTTACAAAAGGTAGATATTTAAGTATCACTCCAGTTAGGGGTTTAGAAAAAATGGTTGCAGGTAAAAATCCTGAAA
CAGCTCCTGTTTTATGTCAAAGGATTTGTGGTGTCTGCCCAATACCACATACTTTAGCTTCTGTAGAAGCTATCGACCAT
TCATTAGATATCGAAGTTCCTAAAGCAGGAAAATTATTAAGACAGGCTACTTTAGCTGCACATGGTGTTAACAGTGCTGC
AATTCACCATTTTTTAATTGCACCTGACTTTGTACCTGAAGAAGATTTCACTACTGCAGTTAAAAGTGTATCTGCTGTTA
GGAAAAATGTACAGTATGTAGTTGATATGCTTGCTGGTGAAGGTATTCACCCATCTGATATTAGAGTAGGTGGTATGGCT
AGAAACATTACTTCAGCTACAAAAGATAAATTGATTGATAGGTTAAAAGCTTTTAAACCGGATATGGAAAAACATGTGGA
ATTAATGAAATCTTTCATTGCTGCAAGAGAACTTCCTGAAGGTTTAGGAGTTGTAGATGCACCACTTTTAGCATCTAGTG
CATCATACGGTAATCTCGATGAATTTGATTATGATAATTTCTCAGAAGTTTTACCTGAAGCTTGGTATGATGATCCGGAA
ATAGGTAAAAAAGCATGTACCACCATTCCTTTAATCAATGGTAAAAACGTTGAAACAGGTCCTAGGGCAAGAATGGATAA
ATTCAACGGATTTAAAGATAAAGGTGTAGTTGCTCAACATGTAGCTAGAGCTGAAGAAATGTTAAATAATTATAATTTAA
CTATTGATCTTCTTGAACAAATTGATACTTCCGCTCCAGCTAGAGCAGATTATGATGACAGAGGAACTGGTAAATTAGGT
ATTGGTGTAATTGAAGGACCTAGGGGTACTAATGTACACATGGCTAATGTTAAAGATAGTAAAATCCAATTCTATTCAGC
TATTGTACCAACTACTTGGAATATTCCAACAATGGGACCAGCTACTGAAGGTTTCCATCATAAATATGGTGCAGATGTTA
TCAGAGCTTACGACCCATGTTTATCATGTGCAACTCACGTAATGGTAGTTGATGATGAGGATAAAAGTATACTTAAAAAC
GATATGGTGAGAATATAA

Upstream 100 bases:

>100_bases
TAGTTTTCTATTTGTAATTTAAACTATATTATATATAGAATATTGGTTATAAATATTAAATGTTAGTTTCTAATTTTTTT
TGAAAAGAGAGGTGACACAT

Downstream 100 bases:

>100_bases
GGAGCTAGGAGGAAATTATATGCCTTATCATGCAGAGACAATCGTAGTGGGATGCGGAAATATTTTATTTAAAGATGATG
GTTTTGGACCATTTGTTATT

Product: coenzyme F420-reducing hydrogenase, alpha subunit, FrhA

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKDRIVISPTSRQEGHAELVMEVDDEGIVTKGRYLSITPVRGLEKMVAGKNPETAPVLCQRICGVCPIPHTLASVEAIDH
SLDIEVPKAGKLLRQATLAAHGVNSAAIHHFLIAPDFVPEEDFTTAVKSVSAVRKNVQYVVDMLAGEGIHPSDIRVGGMA
RNITSATKDKLIDRLKAFKPDMEKHVELMKSFIAARELPEGLGVVDAPLLASSASYGNLDEFDYDNFSEVLPEAWYDDPE
IGKKACTTIPLINGKNVETGPRARMDKFNGFKDKGVVAQHVARAEEMLNNYNLTIDLLEQIDTSAPARADYDDRGTGKLG
IGVIEGPRGTNVHMANVKDSKIQFYSAIVPTTWNIPTMGPATEGFHHKYGADVIRAYDPCLSCATHVMVVDDEDKSILKN
DMVRI

Sequences:

>Translated_405_residues
MKDRIVISPTSRQEGHAELVMEVDDEGIVTKGRYLSITPVRGLEKMVAGKNPETAPVLCQRICGVCPIPHTLASVEAIDH
SLDIEVPKAGKLLRQATLAAHGVNSAAIHHFLIAPDFVPEEDFTTAVKSVSAVRKNVQYVVDMLAGEGIHPSDIRVGGMA
RNITSATKDKLIDRLKAFKPDMEKHVELMKSFIAARELPEGLGVVDAPLLASSASYGNLDEFDYDNFSEVLPEAWYDDPE
IGKKACTTIPLINGKNVETGPRARMDKFNGFKDKGVVAQHVARAEEMLNNYNLTIDLLEQIDTSAPARADYDDRGTGKLG
IGVIEGPRGTNVHMANVKDSKIQFYSAIVPTTWNIPTMGPATEGFHHKYGADVIRAYDPCLSCATHVMVVDDEDKSILKN
DMVRI
>Mature_405_residues
MKDRIVISPTSRQEGHAELVMEVDDEGIVTKGRYLSITPVRGLEKMVAGKNPETAPVLCQRICGVCPIPHTLASVEAIDH
SLDIEVPKAGKLLRQATLAAHGVNSAAIHHFLIAPDFVPEEDFTTAVKSVSAVRKNVQYVVDMLAGEGIHPSDIRVGGMA
RNITSATKDKLIDRLKAFKPDMEKHVELMKSFIAARELPEGLGVVDAPLLASSASYGNLDEFDYDNFSEVLPEAWYDDPE
IGKKACTTIPLINGKNVETGPRARMDKFNGFKDKGVVAQHVARAEEMLNNYNLTIDLLEQIDTSAPARADYDDRGTGKLG
IGVIEGPRGTNVHMANVKDSKIQFYSAIVPTTWNIPTMGPATEGFHHKYGADVIRAYDPCLSCATHVMVVDDEDKSILKN
DMVRI

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=130, Percent_Identity=38.4615384615385, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787207, Length=111, Percent_Identity=34.2342342342342, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788832, Length=397, Percent_Identity=22.9219143576826, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 44272; Mature: 44272

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRIVISPTSRQEGHAELVMEVDDEGIVTKGRYLSITPVRGLEKMVAGKNPETAPVLCQ
CCCCEEECCCCCCCCCEEEEEEECCCCCEECCCEEEECCHHHHHHHHCCCCCCHHHHHHH
RICGVCPIPHTLASVEAIDHSLDIEVPKAGKLLRQATLAAHGVNSAAIHHFLIAPDFVPE
HHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHEEECCCCCCC
EDFTTAVKSVSAVRKNVQYVVDMLAGEGIHPSDIRVGGMARNITSATKDKLIDRLKAFKP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCC
DMEKHVELMKSFIAARELPEGLGVVDAPLLASSASYGNLDEFDYDNFSEVLPEAWYDDPE
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHH
IGKKACTTIPLINGKNVETGPRARMDKFNGFKDKGVVAQHVARAEEMLNNYNLTIDLLEQ
HHHHHHCEEEEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHH
IDTSAPARADYDDRGTGKLGIGVIEGPRGTNVHMANVKDSKIQFYSAIVPTTWNIPTMGP
HCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCC
ATEGFHHKYGADVIRAYDPCLSCATHVMVVDDEDKSILKNDMVRI
CCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure
MKDRIVISPTSRQEGHAELVMEVDDEGIVTKGRYLSITPVRGLEKMVAGKNPETAPVLCQ
CCCCEEECCCCCCCCCEEEEEEECCCCCEECCCEEEECCHHHHHHHHCCCCCCHHHHHHH
RICGVCPIPHTLASVEAIDHSLDIEVPKAGKLLRQATLAAHGVNSAAIHHFLIAPDFVPE
HHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHEEECCCCCCC
EDFTTAVKSVSAVRKNVQYVVDMLAGEGIHPSDIRVGGMARNITSATKDKLIDRLKAFKP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCC
DMEKHVELMKSFIAARELPEGLGVVDAPLLASSASYGNLDEFDYDNFSEVLPEAWYDDPE
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHH
IGKKACTTIPLINGKNVETGPRARMDKFNGFKDKGVVAQHVARAEEMLNNYNLTIDLLEQ
HHHHHHCEEEEECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHH
IDTSAPARADYDDRGTGKLGIGVIEGPRGTNVHMANVKDSKIQFYSAIVPTTWNIPTMGP
HCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCC
ATEGFHHKYGADVIRAYDPCLSCATHVMVVDDEDKSILKNDMVRI
CCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2207102; 9371463; 3663585 [H]