Click here to switch to the map view.

The map label for this gene is mvhA [H]

Identifier: 148643059

GI number: 148643059

Start: 964488

End: 965912

Strand: Reverse

Name: mvhA [H]

Synonym: Msm_0999

Alternate gene names: 148643059

Gene position: 965912-964488 (Counterclockwise)

Preceding gene: 148643060

Following gene: 148643058

Centisome position: 52.12

GC content: 36.7

Gene sequence:

>1425_bases
ATGGTAAAACTTACTATGGAGCCTGTAACACGTATTGAAGGTCACGCTAAAATTACTGTACAGCTCGATGATGATGGAAA
TGTAGTCGATACAAGATTACATGTTATGGAATTCAGAGGTTTCGAAAAATTCTTACAAGGTCGTCCAGTTGAAGAATTAG
CTAGGATTGTACCAAGAATTTGTGGTATTTGTGATGTTCAACACCACTTAGCAGCTGCAAAAGCAGTTGACCAAATTTTC
GGTTTCGAAGATGAAAAAATCTTACCAACAGCATATAAAATGAGAGAAATTATGAACTGGGGTTCATTCATGCACTCCCA
CGCACTTCATTTCTACTTCTTAGCTGCTCCTGATTTAATCATCCCTGACGGAACCAGAAAAACCAGAAATGTTTTCCAAA
TTATTAAAGATCAACCTGAAATTGCACTTCAAGCAATTGAACTCAGAAGAAATGGTTTAGAAATTGTAAGAAAAATCGGT
GGTCGTCCAATTCACCCAATTTCCTCTACTCCTGGAGGTATTTCAACTGAATTAGATGATGAAACTCAAAAAGATTTATT
ACAAAGAGCTCAAAGAAACGTAGAATTAGCACAAGCTACTCTCGACTTAGCTAAACCTATTTTCGAAGAAAAAATAGACT
TAGTCAATTCTTTAGGTTACTTTGGTGACACCAGACACTGTGGTCTTGTAAATGACGGTGTATGGGATGTTTACAACGGT
GACGTAAGAATAAAAGATGTTGATGGTAGTATCTACACTGAATACAAAAACTTAGAATACAAAGATGTTGTTGCTGAACA
TGTAAAACCTTACTCTTGGTTAAAATTCCCTTACATTAAAGAATTAGGATACCCAGAAGGTATTTACAGAGTAGCACCAT
TATCCAGGATTAACGTTGCTGATAAAATGCCAGATGCAGCACCTCTTGCACAAGCGGCATTTGAAGAATTCCATGACAAA
TTTGGATATGCACAAGCACCATTATTATTCCACTGGGCAAGACTTATTGAATTATTAGCATCTGCTGAATGTGCTGCTGA
TGCATTAGACCAAGATTTATCTGGTCCAAAATTCCCAGAAGAATTAGAAAGAACTGCTGGTGAAGGTGCAGGTATTGTAG
AAGCTGCTCGTGGTACTTTAATACACAACTACGCATGTGATGACAATGGTTTAGTAACTGAAGCTAACATTGTTGTAGCT
ACTATCCAAAACAACCCAGCTATGGAAATGGGTATTCAACAAGTAGCTAAAGATTACATCAAACCTGGTGTAGAAGTAGA
CGATAAAATATTCAACTTAATGGAAATGGTTATCAGAGCATATGACCCATGTTTATCCTGTGCAACTCACACTATGGATA
GCCAAATGAGATTAGCTGAAGTAGATATTGTAGACAGTGAAGGAAACATCATTAAAAAATTCTAA

Upstream 100 bases:

>100_bases
ACTGTAGCAGATCAATTAGATGATATTGTAGGTACTTTCTATACTTACACATTACCTGCTGCTTTAGTCCCTATGAAAAT
GCAGAAAGGAGGAGAATAAT

Downstream 100 bases:

>100_bases
TTGAGGGGTTTTAATAATGATAGTATTTAATGAAGATGGCTGTATTAGGTGCGGTGCTTGTGAAGGTACCTGCCCAACCT
CAGCTATTGATGTAACACCT

Product: methyl viologen-reducing hydrogenase, alpha subunit, MvhA

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: Methyl viologen-reducing hydrogenase subunit alpha; MVH subunit A [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MVKLTMEPVTRIEGHAKITVQLDDDGNVVDTRLHVMEFRGFEKFLQGRPVEELARIVPRICGICDVQHHLAAAKAVDQIF
GFEDEKILPTAYKMREIMNWGSFMHSHALHFYFLAAPDLIIPDGTRKTRNVFQIIKDQPEIALQAIELRRNGLEIVRKIG
GRPIHPISSTPGGISTELDDETQKDLLQRAQRNVELAQATLDLAKPIFEEKIDLVNSLGYFGDTRHCGLVNDGVWDVYNG
DVRIKDVDGSIYTEYKNLEYKDVVAEHVKPYSWLKFPYIKELGYPEGIYRVAPLSRINVADKMPDAAPLAQAAFEEFHDK
FGYAQAPLLFHWARLIELLASAECAADALDQDLSGPKFPEELERTAGEGAGIVEAARGTLIHNYACDDNGLVTEANIVVA
TIQNNPAMEMGIQQVAKDYIKPGVEVDDKIFNLMEMVIRAYDPCLSCATHTMDSQMRLAEVDIVDSEGNIIKKF

Sequences:

>Translated_474_residues
MVKLTMEPVTRIEGHAKITVQLDDDGNVVDTRLHVMEFRGFEKFLQGRPVEELARIVPRICGICDVQHHLAAAKAVDQIF
GFEDEKILPTAYKMREIMNWGSFMHSHALHFYFLAAPDLIIPDGTRKTRNVFQIIKDQPEIALQAIELRRNGLEIVRKIG
GRPIHPISSTPGGISTELDDETQKDLLQRAQRNVELAQATLDLAKPIFEEKIDLVNSLGYFGDTRHCGLVNDGVWDVYNG
DVRIKDVDGSIYTEYKNLEYKDVVAEHVKPYSWLKFPYIKELGYPEGIYRVAPLSRINVADKMPDAAPLAQAAFEEFHDK
FGYAQAPLLFHWARLIELLASAECAADALDQDLSGPKFPEELERTAGEGAGIVEAARGTLIHNYACDDNGLVTEANIVVA
TIQNNPAMEMGIQQVAKDYIKPGVEVDDKIFNLMEMVIRAYDPCLSCATHTMDSQMRLAEVDIVDSEGNIIKKF
>Mature_474_residues
MVKLTMEPVTRIEGHAKITVQLDDDGNVVDTRLHVMEFRGFEKFLQGRPVEELARIVPRICGICDVQHHLAAAKAVDQIF
GFEDEKILPTAYKMREIMNWGSFMHSHALHFYFLAAPDLIIPDGTRKTRNVFQIIKDQPEIALQAIELRRNGLEIVRKIG
GRPIHPISSTPGGISTELDDETQKDLLQRAQRNVELAQATLDLAKPIFEEKIDLVNSLGYFGDTRHCGLVNDGVWDVYNG
DVRIKDVDGSIYTEYKNLEYKDVVAEHVKPYSWLKFPYIKELGYPEGIYRVAPLSRINVADKMPDAAPLAQAAFEEFHDK
FGYAQAPLLFHWARLIELLASAECAADALDQDLSGPKFPEELERTAGEGAGIVEAARGTLIHNYACDDNGLVTEANIVVA
TIQNNPAMEMGIQQVAKDYIKPGVEVDDKIFNLMEMVIRAYDPCLSCATHTMDSQMRLAEVDIVDSEGNIIKKF

Specific function: Part of a complex that provides reducing equivalents for heterodisulfide reductase [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=143, Percent_Identity=33.5664335664336, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789368, Length=118, Percent_Identity=31.3559322033898, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 53184; Mature: 53184

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLTMEPVTRIEGHAKITVQLDDDGNVVDTRLHVMEFRGFEKFLQGRPVEELARIVPRI
CEEEECCHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CGICDVQHHLAAAKAVDQIFGFEDEKILPTAYKMREIMNWGSFMHSHALHFYFLAAPDLI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEE
IPDGTRKTRNVFQIIKDQPEIALQAIELRRNGLEIVRKIGGRPIHPISSTPGGISTELDD
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ETQKDLLQRAQRNVELAQATLDLAKPIFEEKIDLVNSLGYFGDTRHCGLVNDGVWDVYNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECC
DVRIKDVDGSIYTEYKNLEYKDVVAEHVKPYSWLKFPYIKELGYPEGIYRVAPLSRINVA
CEEEEECCCCEEEHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCCCEEECCCHHCCHH
DKMPDAAPLAQAAFEEFHDKFGYAQAPLLFHWARLIELLASAECAADALDQDLSGPKFPE
HCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ELERTAGEGAGIVEAARGTLIHNYACDDNGLVTEANIVVATIQNNPAMEMGIQQVAKDYI
HHHHHCCCCCCHHHHHCCCEEEEEEECCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHH
KPGVEVDDKIFNLMEMVIRAYDPCLSCATHTMDSQMRLAEVDIVDSEGNIIKKF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECC
>Mature Secondary Structure
MVKLTMEPVTRIEGHAKITVQLDDDGNVVDTRLHVMEFRGFEKFLQGRPVEELARIVPRI
CEEEECCHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CGICDVQHHLAAAKAVDQIFGFEDEKILPTAYKMREIMNWGSFMHSHALHFYFLAAPDLI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEE
IPDGTRKTRNVFQIIKDQPEIALQAIELRRNGLEIVRKIGGRPIHPISSTPGGISTELDD
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ETQKDLLQRAQRNVELAQATLDLAKPIFEEKIDLVNSLGYFGDTRHCGLVNDGVWDVYNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECC
DVRIKDVDGSIYTEYKNLEYKDVVAEHVKPYSWLKFPYIKELGYPEGIYRVAPLSRINVA
CEEEEECCCCEEEHHCCCCHHHHHHHHCCCCHHHCCHHHHHCCCCCCCEEECCCHHCCHH
DKMPDAAPLAQAAFEEFHDKFGYAQAPLLFHWARLIELLASAECAADALDQDLSGPKFPE
HCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ELERTAGEGAGIVEAARGTLIHNYACDDNGLVTEANIVVATIQNNPAMEMGIQQVAKDYI
HHHHHCCCCCCHHHHHCCCEEEEEEECCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHH
KPGVEVDDKIFNLMEMVIRAYDPCLSCATHTMDSQMRLAEVDIVDSEGNIIKKF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2654933; 9371463 [H]