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The map label for this gene is kdsB [C]

Identifier: 148643004

GI number: 148643004

Start: 906145

End: 907812

Strand: Reverse

Name: kdsB [C]

Synonym: Msm_0944

Alternate gene names: 148643004

Gene position: 907812-906145 (Counterclockwise)

Preceding gene: 148643008

Following gene: 148643000

Centisome position: 48.99

GC content: 31.06

Gene sequence:

>1668_bases
ATGTTTGAAAATAATAAGATATTGGTGGTGATTCCTGCTAGAGGTTATTCAAAAAACATTCCTCGTAAAAATTTACGTTT
ACTTGGTGAAAAACCTCTTATTTATTACTCAATAGATATAGCTAAATCGTCAAAATATGTTGATGATGTAGTGGTGTCTA
CTGAGGATTCTGAAATTGCATCGATTGTTGAAAAGTATGGTACTAGTGTTGTGAAGCGTCCTGTAGAATTAGCTACTGAT
GAAACATTATTGGATACTGTTATTTATGATGCAATGTTACAAAAAGAAAAACAGGCTTTTGACGAATATGATATTGTAAT
TACAATTCAACCTTCTTCTCCGTTACTAAAAACTGAAACATTAGATAAAGCTATTGAAAAATTCGCTGATTTTAATATTG
ATAGTGTAATTTCTGTTGTTGATGATAAGAATCTGAGGTGGGGATTCGATGATGAAAATGGAAGGTATTTTCCATTTTAT
TCTGAAAGATTATATAGGGATTTGCTTCCTAAAACTTTTAAAGAAACCGGAGGTATATTAGCTACTCGCCGTAATTTTGT
AACTGAAACATCTCGTTTAGGTTTGAATATAGACCTGATTGAGATTTCTCGTGAAGAAAGTGTTGAAATAAATACTTATG
AAGACTGGTGGATTGCAAATAATTATCTTAATAAAATAAAAATAGCTTTTGTTGTTGATGCTTATGACCAGATTGGAACA
GGTCATATGTACCGCTGTTTGTCAATGGCATCAAAACTTGTATTCCATGACGTAGTATTCTTTATTAATAGAACCCATCA
GTTGGGAATTGACATTATTGAAGGGTATAATTACAAATATCAAACATATGGAGGTAAATCTGAGTTGCTTGGTTTATTTG
AACAGTTTGATCCGAAGATTATTATTAATGATGTGGGTAATACTTCTTATGAGTATATGGTCGATTTGACAAATAAGGGT
TATTATATAATTAATTTTGAAGATTTTGGAAGCGGTTCAGATCTTGCGGATCTGGTATTTGATTCATTATATGAACATGA
AGGTGATGAAAAATTCTTTGTGGGGCATGAATATTACATTTTAAAAGATGAATTTTATTTGCATCAGCCAAAAATAATTA
CAAATGATGTTAAAACTGTTTTGATTGATTTTGCTCCAAATGATACACTTAACTTGTCTCAAAAAGTTTTGGATGCTCTT
TTAGCTAGTGGGTTTTCTGGCAGAATCAATGTTATTTTAGGTTCAGGACATGAAAATTTTGAAGAATTGTCAGCTAAATA
TGAGCTTATGCAAAATGTTCAGTTTTATACAACAGTAAATTCTATCAGTGACTTCATGATTTCTGCAGATGTTATTTTCA
CTTCTGGGGGAAGAATCATGTATCAGGTCTGTTCTTTAGGTGTTCCATGTATTGTTATATGTAAGGATGAAAGAGAAGAG
AAAAATCTATTTGGAAGTCCTGAACACGGATTTGTAAATATGGGTCTTGGATCTTATTTGACTCAGGAAGACATTATAGA
ACAGTTTAATATTGTGGCTAATGATTTTGAACTTAGACAGGCAATGAATAACAAAATGTTGGAAATTGATTTAAAACATG
GTTTTGAAGAATGTTGGAGTGTTGTTAAGTCTAAGTATAGGGAATTTAATTTGGATAATGCTAAATAA

Upstream 100 bases:

>100_bases
TTTGTCATATTTTTCATTCATTAACACCTAAAAGATTTAATATTAGTATTATTATATTATTTAGTAACGTTTAAATAAAT
TTTGTAATTTAGGGATTTAT

Downstream 100 bases:

>100_bases
ATTCTTTACAATTCTTTTTTTTAAAAAACAGTATTTAATTTAAAAAATAAAAAAAGTTAAAATTCTGAAGAACAGTCTGC
AGAATATTTGTTAAGTAAAA

Product: CMP-N-acetylneuraminic acid synthetase, NeuA

Products: CMP-3-deoxy-D-manno-octulosonate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MFENNKILVVIPARGYSKNIPRKNLRLLGEKPLIYYSIDIAKSSKYVDDVVVSTEDSEIASIVEKYGTSVVKRPVELATD
ETLLDTVIYDAMLQKEKQAFDEYDIVITIQPSSPLLKTETLDKAIEKFADFNIDSVISVVDDKNLRWGFDDENGRYFPFY
SERLYRDLLPKTFKETGGILATRRNFVTETSRLGLNIDLIEISREESVEINTYEDWWIANNYLNKIKIAFVVDAYDQIGT
GHMYRCLSMASKLVFHDVVFFINRTHQLGIDIIEGYNYKYQTYGGKSELLGLFEQFDPKIIINDVGNTSYEYMVDLTNKG
YYIINFEDFGSGSDLADLVFDSLYEHEGDEKFFVGHEYYILKDEFYLHQPKIITNDVKTVLIDFAPNDTLNLSQKVLDAL
LASGFSGRINVILGSGHENFEELSAKYELMQNVQFYTTVNSISDFMISADVIFTSGGRIMYQVCSLGVPCIVICKDEREE
KNLFGSPEHGFVNMGLGSYLTQEDIIEQFNIVANDFELRQAMNNKMLEIDLKHGFEECWSVVKSKYREFNLDNAK

Sequences:

>Translated_555_residues
MFENNKILVVIPARGYSKNIPRKNLRLLGEKPLIYYSIDIAKSSKYVDDVVVSTEDSEIASIVEKYGTSVVKRPVELATD
ETLLDTVIYDAMLQKEKQAFDEYDIVITIQPSSPLLKTETLDKAIEKFADFNIDSVISVVDDKNLRWGFDDENGRYFPFY
SERLYRDLLPKTFKETGGILATRRNFVTETSRLGLNIDLIEISREESVEINTYEDWWIANNYLNKIKIAFVVDAYDQIGT
GHMYRCLSMASKLVFHDVVFFINRTHQLGIDIIEGYNYKYQTYGGKSELLGLFEQFDPKIIINDVGNTSYEYMVDLTNKG
YYIINFEDFGSGSDLADLVFDSLYEHEGDEKFFVGHEYYILKDEFYLHQPKIITNDVKTVLIDFAPNDTLNLSQKVLDAL
LASGFSGRINVILGSGHENFEELSAKYELMQNVQFYTTVNSISDFMISADVIFTSGGRIMYQVCSLGVPCIVICKDEREE
KNLFGSPEHGFVNMGLGSYLTQEDIIEQFNIVANDFELRQAMNNKMLEIDLKHGFEECWSVVKSKYREFNLDNAK
>Mature_555_residues
MFENNKILVVIPARGYSKNIPRKNLRLLGEKPLIYYSIDIAKSSKYVDDVVVSTEDSEIASIVEKYGTSVVKRPVELATD
ETLLDTVIYDAMLQKEKQAFDEYDIVITIQPSSPLLKTETLDKAIEKFADFNIDSVISVVDDKNLRWGFDDENGRYFPFY
SERLYRDLLPKTFKETGGILATRRNFVTETSRLGLNIDLIEISREESVEINTYEDWWIANNYLNKIKIAFVVDAYDQIGT
GHMYRCLSMASKLVFHDVVFFINRTHQLGIDIIEGYNYKYQTYGGKSELLGLFEQFDPKIIINDVGNTSYEYMVDLTNKG
YYIINFEDFGSGSDLADLVFDSLYEHEGDEKFFVGHEYYILKDEFYLHQPKIITNDVKTVLIDFAPNDTLNLSQKVLDAL
LASGFSGRINVILGSGHENFEELSAKYELMQNVQFYTTVNSISDFMISADVIFTSGGRIMYQVCSLGVPCIVICKDEREE
KNLFGSPEHGFVNMGLGSYLTQEDIIEQFNIVANDFELRQAMNNKMLEIDLKHGFEECWSVVKSKYREFNLDNAK

Specific function: Activates Kdo (A Required 8-Carbon Sugar) For Incorporation Into Bacterial Lipopolysaccharide In Gram-Negative Bacteria (By Similarity). [C]

COG id: COG1083

COG function: function code M; CMP-N-acetylneuraminic acid synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI8923900, Length=254, Percent_Identity=29.5275590551181, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR007235
- InterPro:   IPR020023 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF04101 Glyco_tran_28_C [H]

EC number: 2.7.7.38 [C]

Molecular weight: Translated: 63742; Mature: 63742

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENNKILVVIPARGYSKNIPRKNLRLLGEKPLIYYSIDIAKSSKYVDDVVVSTEDSEIA
CCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECCCHHHH
SIVEKYGTSVVKRPVELATDETLLDTVIYDAMLQKEKQAFDEYDIVITIQPSSPLLKTET
HHHHHHCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
LDKAIEKFADFNIDSVISVVDDKNLRWGFDDENGRYFPFYSERLYRDLLPKTFKETGGIL
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
ATRRNFVTETSRLGLNIDLIEISREESVEINTYEDWWIANNYLNKIKIAFVVDAYDQIGT
EECHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCEEECCCCCEEEEEEEEECHHHCCC
GHMYRCLSMASKLVFHDVVFFINRTHQLGIDIIEGYNYKYQTYGGKSELLGLFEQFDPKI
CHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCEEEEECCCHHHHHHHHHHCCCEE
IINDVGNTSYEYMVDLTNKGYYIINFEDFGSGSDLADLVFDSLYEHEGDEKFFVGHEYYI
EEECCCCCCEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCEEEE
LKDEFYLHQPKIITNDVKTVLIDFAPNDTLNLSQKVLDALLASGFSGRINVILGSGHENF
EECCEEEECCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EELSAKYELMQNVQFYTTVNSISDFMISADVIFTSGGRIMYQVCSLGVPCIVICKDEREE
HHHHHHHHHHHCCEEEEEHHHHHHHHEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCH
KNLFGSPEHGFVNMGLGSYLTQEDIIEQFNIVANDFELRQAMNNKMLEIDLKHGFEECWS
HCCCCCCCCCEEECCCCHHCCHHHHHHHHHHHHCHHHHHHHHCCCEEEEEHHCCHHHHHH
VVKSKYREFNLDNAK
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MFENNKILVVIPARGYSKNIPRKNLRLLGEKPLIYYSIDIAKSSKYVDDVVVSTEDSEIA
CCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEECCCHHHH
SIVEKYGTSVVKRPVELATDETLLDTVIYDAMLQKEKQAFDEYDIVITIQPSSPLLKTET
HHHHHHCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHH
LDKAIEKFADFNIDSVISVVDDKNLRWGFDDENGRYFPFYSERLYRDLLPKTFKETGGIL
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
ATRRNFVTETSRLGLNIDLIEISREESVEINTYEDWWIANNYLNKIKIAFVVDAYDQIGT
EECHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCEEECCCCCEEEEEEEEECHHHCCC
GHMYRCLSMASKLVFHDVVFFINRTHQLGIDIIEGYNYKYQTYGGKSELLGLFEQFDPKI
CHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEECCCEEEEECCCHHHHHHHHHHCCCEE
IINDVGNTSYEYMVDLTNKGYYIINFEDFGSGSDLADLVFDSLYEHEGDEKFFVGHEYYI
EEECCCCCCEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCEEEE
LKDEFYLHQPKIITNDVKTVLIDFAPNDTLNLSQKVLDALLASGFSGRINVILGSGHENF
EECCEEEECCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EELSAKYELMQNVQFYTTVNSISDFMISADVIFTSGGRIMYQVCSLGVPCIVICKDEREE
HHHHHHHHHHHCCEEEEEHHHHHHHHEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCH
KNLFGSPEHGFVNMGLGSYLTQEDIIEQFNIVANDFELRQAMNNKMLEIDLKHGFEECWS
HCCCCCCCCCEEECCCCHHCCHHHHHHHHHHHHCHHHHHHHHCCCEEEEEHHCCHHHHHH
VVKSKYREFNLDNAK
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: GSH [C]

Metal ions: Ba2+; Ca2+; Cd2+; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.34 {dCTP}} 0.88 {UTP}} 0.8 {3-deoxy-manno-octulosonate}} 0.39 {3-deoxy-manno-octulosonate}} 0.29 {3-deoxy-manno-octulosonate}} 0.22 {CTP}} 0.2 {CTP}} [C]

Substrates: CTP; 3-Deoxy-D-manno-2-octulosonate [C]

Specific reaction: CTP + 3-Deoxy-D-manno-2-octulosonate --> CMP-3-deoxy-D-manno-octulosonate + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 2,6-Anhydro-3-deoxy-D-glycero-D-talo-octanoate; Diphosphate; Hg2+; 3-deoxy-manno-octulosonate 8-phosphate; N-acetyl neuraminate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]