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The map label for this gene is pycB [H]

Identifier: 148642999

GI number: 148642999

Start: 898993

End: 900702

Strand: Reverse

Name: pycB [H]

Synonym: Msm_0939

Alternate gene names: 148642999

Gene position: 900702-898993 (Counterclockwise)

Preceding gene: 148643000

Following gene: 148642998

Centisome position: 48.6

GC content: 34.44

Gene sequence:

>1710_bases
ATGACTAAAGTAAGATTTACCGAAACTGCACTTCGTGATGCTCACCAATCCTTACTTGCAACTAGGATGAGAACCAGGGA
TATGATTCCTATTGCTGAAGAAATGGATAAAATAGGTTTTTTCTCTGTTGAAGCATGGGGTGGAGCTACTTTTGATACAT
GTATAAGATATTTAAACGAAGACCCATGGGAAAGGTTAAGAAGCTTAAAATCTGAATTTACTAAAACTCCAATTCAGATG
TTAATTAGGGGACAAAATTTAGTAGGATATAAACATTATCCTGATGACATTGTTACAAAATTTGTAGAAAAATCCTATGA
AAATGGTGTTGACATATTTAGAGTTTTTGATGCTTTAAATGATGTTCGTAACATGAAACAAACAATTAAAGTTGCTAAAG
ATCAAGGAGCTCATGTACAAGGGACTATAAGTTATACTTTAAGTCCTGTTCATACTTTAGATGATTTTGTGGATTTAGCT
AAAGAACTTGAAGCTCTTGACTGTGATTCAGTATCTATTAAAGATATGGCTGGTTTAATAACTCCTTCAGCAGCTTATGA
ATTAGTAAGCAGATTAAAAGAAGAAACTGATTTATTGGTTGATTTGCATTGTCACTGTACTAGTGGAATGACTCAAATCA
GTTATTATGCAGCTTGTCAGGCAGGTGTAGATGTTTTAGACACAGCTATTTCCCCACTTTCCGGAGGAACAAGCCAGCCG
CCTACTGAAAGTATGGTTGCTGCATTACAGGGAACTCCTTATGATACTGAATTGGATTTAAAATCATTAACCGCTATTAA
GAAATACTTTGATCAAATTAAAGAAAAATATTTGGCTTTAATTGATCCTATTGCTGAAAGTATTGATACTGATGTTTTAA
TGTATCAAATCCCTGGAGGAATGCTTTCAAACTTAATTTCACAGCTTAAAGAACAAAATGCTTTAGATAGATATAATGAT
GTACTTGATGAAATGCCTCGTGTAAGAAAAGACATGGGTTACCCACCTCTTGTAACTCCAACTAGTCAAATTGTAGGTAT
CCAGGCTGTAATGAATGTATTAGGTGGAGAAAGGTACAAAACAGTTTCCAATGAAGTAAAAGAATATATGAAAGGTTTCT
ATGGTAAACCTCCTGCACCAGTTAATGCTAAAATAGCTAATAAAATTATTGGTGATGAAGAAGTTATTACCTGCAGGCCT
GCAGATTTACTTGAAGATGAATTTGACAAATATAAAACAGAAGGGGAACAAAAAGGTTTTGTCAAATCTGATGAAGATGC
ACTTACTTATGCCTTATATCCATCAATCGCTCCAAAATTCCTTAAAGGAGAAGCAGTTGAAGAAGAACTTAAAAGTGTTG
CTCTTCCAAGCGATAATGAAATTGGAATTCCAACTCAATATAATGTTGAAGTTGACGGAGACGTATATGAAGTTAAAATC
ATGCCTACCGGATTTATGGAAGTTGAAGAAACTGAAAAAGGACCATTCACTCCTGTTGAAGGTGGATTAATTTCAACTAT
GCAGGGTATGGTTATTAAACTTACCGTAAATGTTGGGGATAAAGTAACTAAAGGATCAACTGTGGCGGTCATTGAAGCTA
TGAAAATGGAAAATGATATTCAGGCTGAAGAAGACGGTGTTGTAAAAGAAATCTTTGTAGAAGCTGGAGATGCAGTTAAT
TCCGGTGATATTCTAATGGTTATTGAATAG

Upstream 100 bases:

>100_bases
AAACAAACATATTAATACTAGTTAAAATAATATATAATATTTGGTATAATTTATTCTACTTATCGATTTATAAAAAACAA
TTAATGAGAGGTGTTAATCT

Downstream 100 bases:

>100_bases
GATAACCATTTAATTTTTTTTCTTTTATAAGTTCACTAAATAAGAATATTTATATACTTAATGAGAAATAATCTTATTTG
ATGATGTGTTTTGGATAAGA

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MTKVRFTETALRDAHQSLLATRMRTRDMIPIAEEMDKIGFFSVEAWGGATFDTCIRYLNEDPWERLRSLKSEFTKTPIQM
LIRGQNLVGYKHYPDDIVTKFVEKSYENGVDIFRVFDALNDVRNMKQTIKVAKDQGAHVQGTISYTLSPVHTLDDFVDLA
KELEALDCDSVSIKDMAGLITPSAAYELVSRLKEETDLLVDLHCHCTSGMTQISYYAACQAGVDVLDTAISPLSGGTSQP
PTESMVAALQGTPYDTELDLKSLTAIKKYFDQIKEKYLALIDPIAESIDTDVLMYQIPGGMLSNLISQLKEQNALDRYND
VLDEMPRVRKDMGYPPLVTPTSQIVGIQAVMNVLGGERYKTVSNEVKEYMKGFYGKPPAPVNAKIANKIIGDEEVITCRP
ADLLEDEFDKYKTEGEQKGFVKSDEDALTYALYPSIAPKFLKGEAVEEELKSVALPSDNEIGIPTQYNVEVDGDVYEVKI
MPTGFMEVEETEKGPFTPVEGGLISTMQGMVIKLTVNVGDKVTKGSTVAVIEAMKMENDIQAEEDGVVKEIFVEAGDAVN
SGDILMVIE

Sequences:

>Translated_569_residues
MTKVRFTETALRDAHQSLLATRMRTRDMIPIAEEMDKIGFFSVEAWGGATFDTCIRYLNEDPWERLRSLKSEFTKTPIQM
LIRGQNLVGYKHYPDDIVTKFVEKSYENGVDIFRVFDALNDVRNMKQTIKVAKDQGAHVQGTISYTLSPVHTLDDFVDLA
KELEALDCDSVSIKDMAGLITPSAAYELVSRLKEETDLLVDLHCHCTSGMTQISYYAACQAGVDVLDTAISPLSGGTSQP
PTESMVAALQGTPYDTELDLKSLTAIKKYFDQIKEKYLALIDPIAESIDTDVLMYQIPGGMLSNLISQLKEQNALDRYND
VLDEMPRVRKDMGYPPLVTPTSQIVGIQAVMNVLGGERYKTVSNEVKEYMKGFYGKPPAPVNAKIANKIIGDEEVITCRP
ADLLEDEFDKYKTEGEQKGFVKSDEDALTYALYPSIAPKFLKGEAVEEELKSVALPSDNEIGIPTQYNVEVDGDVYEVKI
MPTGFMEVEETEKGPFTPVEGGLISTMQGMVIKLTVNVGDKVTKGSTVAVIEAMKMENDIQAEEDGVVKEIFVEAGDAVN
SGDILMVIE
>Mature_568_residues
TKVRFTETALRDAHQSLLATRMRTRDMIPIAEEMDKIGFFSVEAWGGATFDTCIRYLNEDPWERLRSLKSEFTKTPIQML
IRGQNLVGYKHYPDDIVTKFVEKSYENGVDIFRVFDALNDVRNMKQTIKVAKDQGAHVQGTISYTLSPVHTLDDFVDLAK
ELEALDCDSVSIKDMAGLITPSAAYELVSRLKEETDLLVDLHCHCTSGMTQISYYAACQAGVDVLDTAISPLSGGTSQPP
TESMVAALQGTPYDTELDLKSLTAIKKYFDQIKEKYLALIDPIAESIDTDVLMYQIPGGMLSNLISQLKEQNALDRYNDV
LDEMPRVRKDMGYPPLVTPTSQIVGIQAVMNVLGGERYKTVSNEVKEYMKGFYGKPPAPVNAKIANKIIGDEEVITCRPA
DLLEDEFDKYKTEGEQKGFVKSDEDALTYALYPSIAPKFLKGEAVEEELKSVALPSDNEIGIPTQYNVEVDGDVYEVKIM
PTGFMEVEETEKGPFTPVEGGLISTMQGMVIKLTVNVGDKVTKGSTVAVIEAMKMENDIQAEEDGVVKEIFVEAGDAVNS
GDILMVIE

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=619, Percent_Identity=33.1179321486268, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI106049295, Length=619, Percent_Identity=33.1179321486268, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI106049292, Length=619, Percent_Identity=33.1179321486268, Blast_Score=290, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17562816, Length=616, Percent_Identity=32.3051948051948, Blast_Score=284, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319695, Length=618, Percent_Identity=32.3624595469256, Blast_Score=297, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6321376, Length=617, Percent_Identity=32.7390599675851, Blast_Score=295, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24652212, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652210, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652214, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI19921944, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652216, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI281363050, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652224, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652222, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652220, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24652218, Length=612, Percent_Identity=34.1503267973856, Blast_Score=308, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 63082; Mature: 62950

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVRFTETALRDAHQSLLATRMRTRDMIPIAEEMDKIGFFSVEAWGGATFDTCIRYLNE
CCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHCC
DPWERLRSLKSEFTKTPIQMLIRGQNLVGYKHYPDDIVTKFVEKSYENGVDIFRVFDALN
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
DVRNMKQTIKVAKDQGAHVQGTISYTLSPVHTLDDFVDLAKELEALDCDSVSIKDMAGLI
HHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
TPSAAYELVSRLKEETDLLVDLHCHCTSGMTQISYYAACQAGVDVLDTAISPLSGGTSQP
CCHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PTESMVAALQGTPYDTELDLKSLTAIKKYFDQIKEKYLALIDPIAESIDTDVLMYQIPGG
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
MLSNLISQLKEQNALDRYNDVLDEMPRVRKDMGYPPLVTPTSQIVGIQAVMNVLGGERYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
TVSNEVKEYMKGFYGKPPAPVNAKIANKIIGDEEVITCRPADLLEDEFDKYKTEGEQKGF
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCC
VKSDEDALTYALYPSIAPKFLKGEAVEEELKSVALPSDNEIGIPTQYNVEVDGDVYEVKI
CCCCCCCEEEEECCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEE
MPTGFMEVEETEKGPFTPVEGGLISTMQGMVIKLTVNVGDKVTKGSTVAVIEAMKMENDI
ECCCCEEHHCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCC
QAEEDGVVKEIFVEAGDAVNSGDILMVIE
CCCCCCHHHHHHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure 
TKVRFTETALRDAHQSLLATRMRTRDMIPIAEEMDKIGFFSVEAWGGATFDTCIRYLNE
CCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHCC
DPWERLRSLKSEFTKTPIQMLIRGQNLVGYKHYPDDIVTKFVEKSYENGVDIFRVFDALN
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
DVRNMKQTIKVAKDQGAHVQGTISYTLSPVHTLDDFVDLAKELEALDCDSVSIKDMAGLI
HHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
TPSAAYELVSRLKEETDLLVDLHCHCTSGMTQISYYAACQAGVDVLDTAISPLSGGTSQP
CCHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PTESMVAALQGTPYDTELDLKSLTAIKKYFDQIKEKYLALIDPIAESIDTDVLMYQIPGG
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
MLSNLISQLKEQNALDRYNDVLDEMPRVRKDMGYPPLVTPTSQIVGIQAVMNVLGGERYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
TVSNEVKEYMKGFYGKPPAPVNAKIANKIIGDEEVITCRPADLLEDEFDKYKTEGEQKGF
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCC
VKSDEDALTYALYPSIAPKFLKGEAVEEELKSVALPSDNEIGIPTQYNVEVDGDVYEVKI
CCCCCCCEEEEECCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEE
MPTGFMEVEETEKGPFTPVEGGLISTMQGMVIKLTVNVGDKVTKGSTVAVIEAMKMENDI
ECCCCEEHHCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCC
QAEEDGVVKEIFVEAGDAVNSGDILMVIE
CCCCCCHHHHHHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]