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The map label for this gene is pgk [H]

Identifier: 148642978

GI number: 148642978

Start: 882283

End: 883515

Strand: Reverse

Name: pgk [H]

Synonym: Msm_0918

Alternate gene names: 148642978

Gene position: 883515-882283 (Counterclockwise)

Preceding gene: 148642979

Following gene: 148642975

Centisome position: 47.68

GC content: 35.2

Gene sequence:

>1233_bases
ATGATGGCTGATAAATTTAATACAATTGATGACTTTGATGTTGAAAATAAAACTGTGCTGGTTAGAATTGATATTAATTC
TCCGGTTGATCCTAGTTCTGGAATCATATTGGATGATACAAGATTAAAATTACATGCTAAAACAATTAAAGAATTGTCTA
ATAAAGGAGCTAAAGTTGTATTGCTTGCTCATCAAAGCCGTCCGGGTAAAAAAGATTTCACAGATTTGTCCCAGCATGCT
AATGCATTATCTGACCTTTTGAATTTAAGAGTAAAGTACATAGACTCTATTTTTTCAAAAGCTGCAAAAGAAGCTATTAA
ACAGTTAAAACCTCATGAAATTCTTTTACTTGAAAATGTAAGGTTCTTTTCTGAAGAATCTTTATCCAGACCTGCTGAAG
CTCAGGCAGAAACATTACTTGTTAGGCAACTGTCTCCTGTGATTGATTATTTTGTTAATGATGCATTTGCAGCAGCTCAT
AGATCTCAGGCATCTTTAGTAGGTTTTACTCTCAACACTCCTTCAGCTGCAGGCAGGGTAATGGAAAAAGAACTGACTGT
AATTCAAAATGCACTGGATAATGTGGAACATCCCTGTGTATTCCTGCTTGGAGGAATGAAACCTGATGATTCAATAGATG
TTATGGAAAATGTATTAAGTAACGGTACTGCAGATTCTATCTTAACAACAGGTATTGTAGGAAACATTGTTCTTTGGGCT
GCAGGTGTGGATATCGGTGAGGTAAACAAGAATTTCATAATAAGCAAAGGATATGAATCAATGGTTTCTAAAGCTGAGGA
ACTTATTGAAAAATTCGGAAATAAAGTAAAATACCCTATTGATGTAGCTATTGAAAAAGATGGTGAAAGAAAGGATGTTG
ACTGTGGAGAAATTCCTAATGAATCTATTTTTGACATTGGTGTAAAAACAATCAGTTATTATGCAAAAATAATTCGTGAT
TCAAAAACAGTATTTGCAAACGGTCCTGCAGGTGTATTTGAAGACCCTAAATTTGCAATGGGCACTGAAGATTTGATTAA
TGCTATGGCTAAATCAAATGCATTTACACTGATTGGCGGAGGCCATATTGCTGCGGCTACTTCAGCTTTAGGTTATGAAG
ATCAAATGAGTCATTTAAGTAGTGGTGGCGGAGCTTGCATCAGTATGCTTGCAGGTAAAAAACTGGCTGCCGTTCAGGCA
TTAAGGGATTCAGCAAAAAATAGTAAAAATTAA

Upstream 100 bases:

>100_bases
TGTTAAGGCTAAAAATCCTAAAGAAGCTTTACTTGATTTGGTTAGTAAATTATAAAAACTTGTATTTTTATAAATATCTA
TATTTAATATTTTGAGTGAA

Downstream 100 bases:

>100_bases
CTATTTTTCGCTTTAAATTCTTTTAATAAATTTTCACTAGCTATTTTTGGGTTTTTAGCTTCCATGATTGCACTAACAAC
AGATAATCCATCAATTCCGC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MMADKFNTIDDFDVENKTVLVRIDINSPVDPSSGIILDDTRLKLHAKTIKELSNKGAKVVLLAHQSRPGKKDFTDLSQHA
NALSDLLNLRVKYIDSIFSKAAKEAIKQLKPHEILLLENVRFFSEESLSRPAEAQAETLLVRQLSPVIDYFVNDAFAAAH
RSQASLVGFTLNTPSAAGRVMEKELTVIQNALDNVEHPCVFLLGGMKPDDSIDVMENVLSNGTADSILTTGIVGNIVLWA
AGVDIGEVNKNFIISKGYESMVSKAEELIEKFGNKVKYPIDVAIEKDGERKDVDCGEIPNESIFDIGVKTISYYAKIIRD
SKTVFANGPAGVFEDPKFAMGTEDLINAMAKSNAFTLIGGGHIAAATSALGYEDQMSHLSSGGGACISMLAGKKLAAVQA
LRDSAKNSKN

Sequences:

>Translated_410_residues
MMADKFNTIDDFDVENKTVLVRIDINSPVDPSSGIILDDTRLKLHAKTIKELSNKGAKVVLLAHQSRPGKKDFTDLSQHA
NALSDLLNLRVKYIDSIFSKAAKEAIKQLKPHEILLLENVRFFSEESLSRPAEAQAETLLVRQLSPVIDYFVNDAFAAAH
RSQASLVGFTLNTPSAAGRVMEKELTVIQNALDNVEHPCVFLLGGMKPDDSIDVMENVLSNGTADSILTTGIVGNIVLWA
AGVDIGEVNKNFIISKGYESMVSKAEELIEKFGNKVKYPIDVAIEKDGERKDVDCGEIPNESIFDIGVKTISYYAKIIRD
SKTVFANGPAGVFEDPKFAMGTEDLINAMAKSNAFTLIGGGHIAAATSALGYEDQMSHLSSGGGACISMLAGKKLAAVQA
LRDSAKNSKN
>Mature_410_residues
MMADKFNTIDDFDVENKTVLVRIDINSPVDPSSGIILDDTRLKLHAKTIKELSNKGAKVVLLAHQSRPGKKDFTDLSQHA
NALSDLLNLRVKYIDSIFSKAAKEAIKQLKPHEILLLENVRFFSEESLSRPAEAQAETLLVRQLSPVIDYFVNDAFAAAH
RSQASLVGFTLNTPSAAGRVMEKELTVIQNALDNVEHPCVFLLGGMKPDDSIDVMENVLSNGTADSILTTGIVGNIVLWA
AGVDIGEVNKNFIISKGYESMVSKAEELIEKFGNKVKYPIDVAIEKDGERKDVDCGEIPNESIFDIGVKTISYYAKIIRD
SKTVFANGPAGVFEDPKFAMGTEDLINAMAKSNAFTLIGGGHIAAATSALGYEDQMSHLSSGGGACISMLAGKKLAAVQA
LRDSAKNSKN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=418, Percent_Identity=31.3397129186603, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4505763, Length=421, Percent_Identity=31.8289786223278, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1789294, Length=409, Percent_Identity=31.7848410757946, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=33.1719128329298, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=33.0120481927711, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=33.0120481927711, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24581203, Length=422, Percent_Identity=32.9383886255924, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 44349; Mature: 44349

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMADKFNTIDDFDVENKTVLVRIDINSPVDPSSGIILDDTRLKLHAKTIKELSNKGAKVV
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEE
LLAHQSRPGKKDFTDLSQHANALSDLLNLRVKYIDSIFSKAAKEAIKQLKPHEILLLENV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
RFFSEESLSRPAEAQAETLLVRQLSPVIDYFVNDAFAAAHRSQASLVGFTLNTPSAAGRV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
MEKELTVIQNALDNVEHPCVFLLGGMKPDDSIDVMENVLSNGTADSILTTGIVGNIVLWA
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
AGVDIGEVNKNFIISKGYESMVSKAEELIEKFGNKVKYPIDVAIEKDGERKDVDCGEIPN
CCCCHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
ESIFDIGVKTISYYAKIIRDSKTVFANGPAGVFEDPKFAMGTEDLINAMAKSNAFTLIGG
CCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEC
GHIAAATSALGYEDQMSHLSSGGGACISMLAGKKLAAVQALRDSAKNSKN
CHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMADKFNTIDDFDVENKTVLVRIDINSPVDPSSGIILDDTRLKLHAKTIKELSNKGAKVV
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEE
LLAHQSRPGKKDFTDLSQHANALSDLLNLRVKYIDSIFSKAAKEAIKQLKPHEILLLENV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
RFFSEESLSRPAEAQAETLLVRQLSPVIDYFVNDAFAAAHRSQASLVGFTLNTPSAAGRV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
MEKELTVIQNALDNVEHPCVFLLGGMKPDDSIDVMENVLSNGTADSILTTGIVGNIVLWA
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
AGVDIGEVNKNFIISKGYESMVSKAEELIEKFGNKVKYPIDVAIEKDGERKDVDCGEIPN
CCCCHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
ESIFDIGVKTISYYAKIIRDSKTVFANGPAGVFEDPKFAMGTEDLINAMAKSNAFTLIGG
CCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEC
GHIAAATSALGYEDQMSHLSSGGGACISMLAGKKLAAVQALRDSAKNSKN
CHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]