BacMap

Definition	Methanobrevibacter smithii ATCC 35061 chromosome, complete genome.
Accession	NC_009515
Length	1,853,160

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The map label for this gene is tuf [H]

Identifier: 148642958

GI number: 148642958

Start: 859258

End: 860538

Strand: Reverse

Name: tuf [H]

Synonym: Msm_0898

Alternate gene names: 148642958

Gene position: 860538-859258 (Counterclockwise)

Preceding gene: 148642959

Following gene: 148642957

Centisome position: 46.44

GC content: 38.1

Gene sequence:

>1281_bases
ATGAATGTTATTTCGATTTATACGAAAAGAGGTATTATTATGGCAAAAACTAAAGAACATATTAATTTAGCATTTATCGG
ACACGTTGACCACGGAAAATCCACATTAGTTGGACACTTGTTATTAAAAGCAGGTGCAATCGCTGAACAACAATTAGATG
ATGGAGAAAACAAATTCAGATTTGTTATGGATAAATTAGGAGAAGAAAGGGAAAGAGGAGTAACTATCGACCTTGCTCAC
CAAAAATTCTCCACCAAAAAATACGACTACACTGTTGTAGACTGTCCTGGACACAGAGACTTCGTTAAAAATATGATTAC
TGGTGCTTCCCAAGCAGATGCTGGTGTATTAGTAGTAGCTGCAGACGACGGTGTAATGCCACAAACCAAAGAACACGTTT
TCTTATCCAAAACTTTAGGTATTAACCAATTAATCGTTGCAATCAACAAAATCGATTTAGTAGATTATGATGAAGCAAAA
TTCAACGAATTAAAAGACGAAGTATCTGCTTTAATTAAAACTGTTGGATTCAACCCTGCTGATGTTCCTTTCATCCCTGT
TTCTGCATTTGAAGGAGACAACATTAAAGATGCAAGTCCTAACACCTCCTGGTACAAAGGTGACACTTTAATGCAAGCTT
TAGATAATTTAGCTGCTCCTGAAAAACCTGTATCCTTACCATTAAGGATCCCTATTCAAGATGTTTACTCCATTACTGGT
GTAGGTACTGTACCTGTAGGAAGAGTAGAAACCGGTGTTATGAAAAAAGGAGAAAACGTAATCTTCGAACCTGCTGGAGC
TTCTGGAGAAGTAAAATCTATCGAAATGCACCACGAAACCTTTGAAACTGCTGAACCTGGTGACAACATCGGATTCAATG
TAAGAGGTGTAGGTAAAAACGATATCAGGAGAGGAGACGTAGCTGGTCATGTAGACGATGCTCCTGCTGTAGCTAAAGAA
TTTGATGCACAAATTGTTGTTTTACAACACCCTGGTGTAATCACTGTTGGATACACTCCTGTATTCCACTGTCACACTTC
TCAAGTTGCATGTACTTTCTTAGAATTAACTGCAAAATTAGACCCTGCAACTGGTCAAGTAGCTGAAGAAAACCCTGACT
TCTTAAAAACTGGTAACGCAGCATTCGTAAAAGTTAAACCAACCAAACCTATGGTAATCGAAAACGCTAAAAAAATCCCT
CAAATGGGTAGATTTGCTATTAGGGATATGGGTCAAACTGTTGCTGCTGGATTATGTATTGACGTTACCCCAGCTAAATA
G

Upstream 100 bases:

>100_bases
TTTTAATTTTTTTATATTTTTTATTTAAAATTACGAAAAAAACATTACATATTTATATGTGAATAATTAAAATTTAAATT
AAGCTAAAATTATAAGCTTA

Downstream 100 bases:

>100_bases
ATTTGTAAGTAGTTAATTAAAAGAAAGGGGTTGTTCCTTTCTTTTTTATTTTTATTTTTGGAGGATAATAATGCATCAAG
CAAGAATTAAGCTTACAGGA

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNVISIYTKRGIIMAKTKEHINLAFIGHVDHGKSTLVGHLLLKAGAIAEQQLDDGENKFRFVMDKLGEERERGVTIDLAH
QKFSTKKYDYTVVDCPGHRDFVKNMITGASQADAGVLVVAADDGVMPQTKEHVFLSKTLGINQLIVAINKIDLVDYDEAK
FNELKDEVSALIKTVGFNPADVPFIPVSAFEGDNIKDASPNTSWYKGDTLMQALDNLAAPEKPVSLPLRIPIQDVYSITG
VGTVPVGRVETGVMKKGENVIFEPAGASGEVKSIEMHHETFETAEPGDNIGFNVRGVGKNDIRRGDVAGHVDDAPAVAKE
FDAQIVVLQHPGVITVGYTPVFHCHTSQVACTFLELTAKLDPATGQVAEENPDFLKTGNAAFVKVKPTKPMVIENAKKIP
QMGRFAIRDMGQTVAAGLCIDVTPAK

Sequences:

>Translated_426_residues
MNVISIYTKRGIIMAKTKEHINLAFIGHVDHGKSTLVGHLLLKAGAIAEQQLDDGENKFRFVMDKLGEERERGVTIDLAH
QKFSTKKYDYTVVDCPGHRDFVKNMITGASQADAGVLVVAADDGVMPQTKEHVFLSKTLGINQLIVAINKIDLVDYDEAK
FNELKDEVSALIKTVGFNPADVPFIPVSAFEGDNIKDASPNTSWYKGDTLMQALDNLAAPEKPVSLPLRIPIQDVYSITG
VGTVPVGRVETGVMKKGENVIFEPAGASGEVKSIEMHHETFETAEPGDNIGFNVRGVGKNDIRRGDVAGHVDDAPAVAKE
FDAQIVVLQHPGVITVGYTPVFHCHTSQVACTFLELTAKLDPATGQVAEENPDFLKTGNAAFVKVKPTKPMVIENAKKIP
QMGRFAIRDMGQTVAAGLCIDVTPAK
>Mature_426_residues
MNVISIYTKRGIIMAKTKEHINLAFIGHVDHGKSTLVGHLLLKAGAIAEQQLDDGENKFRFVMDKLGEERERGVTIDLAH
QKFSTKKYDYTVVDCPGHRDFVKNMITGASQADAGVLVVAADDGVMPQTKEHVFLSKTLGINQLIVAINKIDLVDYDEAK
FNELKDEVSALIKTVGFNPADVPFIPVSAFEGDNIKDASPNTSWYKGDTLMQALDNLAAPEKPVSLPLRIPIQDVYSITG
VGTVPVGRVETGVMKKGENVIFEPAGASGEVKSIEMHHETFETAEPGDNIGFNVRGVGKNDIRRGDVAGHVDDAPAVAKE
FDAQIVVLQHPGVITVGYTPVFHCHTSQVACTFLELTAKLDPATGQVAEENPDFLKTGNAAFVKVKPTKPMVIENAKKIP
QMGRFAIRDMGQTVAAGLCIDVTPAK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503475, Length=441, Percent_Identity=48.9795918367347, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI4503471, Length=437, Percent_Identity=48.9702517162471, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI194018522, Length=432, Percent_Identity=34.7222222222222, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI194018520, Length=432, Percent_Identity=34.7222222222222, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI194097354, Length=432, Percent_Identity=34.7222222222222, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=34.965034965035, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=34.965034965035, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI46094014, Length=432, Percent_Identity=33.7962962962963, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI34147630, Length=423, Percent_Identity=33.5697399527187, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=26.4615384615385, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI82546879, Length=412, Percent_Identity=25, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI169162126, Length=138, Percent_Identity=35.5072463768116, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI169162824, Length=138, Percent_Identity=35.5072463768116, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI239752724, Length=138, Percent_Identity=35.5072463768116, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI157426893, Length=258, Percent_Identity=29.0697674418605, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI45593140, Length=422, Percent_Identity=22.7488151658768, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4503507, Length=366, Percent_Identity=24.3169398907104, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI53729339, Length=240, Percent_Identity=29.5833333333333, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI53729337, Length=240, Percent_Identity=29.5833333333333, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=425, Percent_Identity=34.5882352941176, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789737, Length=425, Percent_Identity=34.5882352941176, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789108, Length=324, Percent_Identity=35.8024691358025, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI2367247, Length=288, Percent_Identity=31.9444444444444, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994988, Length=264, Percent_Identity=32.5757575757576, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788922, Length=244, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17552884, Length=437, Percent_Identity=49.8855835240275, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17569207, Length=437, Percent_Identity=49.8855835240275, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=47.4418604651163, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32566629, Length=428, Percent_Identity=32.7102803738318, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI115532067, Length=421, Percent_Identity=34.2042755344418, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI115532065, Length=421, Percent_Identity=34.2042755344418, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17556456, Length=426, Percent_Identity=32.6291079812207, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI25141371, Length=341, Percent_Identity=36.0703812316716, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=51.006711409396, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17557151, Length=240, Percent_Identity=31.6666666666667, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17565438, Length=409, Percent_Identity=23.7163814180929, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17506081, Length=280, Percent_Identity=24.2857142857143, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994658, Length=260, Percent_Identity=32.3076923076923, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509919, Length=373, Percent_Identity=22.7882037533512, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=435, Percent_Identity=50.5747126436782, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6319594, Length=435, Percent_Identity=50.5747126436782, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320377, Length=428, Percent_Identity=32.4766355140187, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324761, Length=418, Percent_Identity=32.5358851674641, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322937, Length=452, Percent_Identity=29.4247787610619, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323320, Length=259, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6324550, Length=181, Percent_Identity=29.8342541436464, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=31.8471337579618, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=31.8471337579618, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652838, Length=437, Percent_Identity=50.1144164759725, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17137572, Length=437, Percent_Identity=50.1144164759725, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45553807, Length=437, Percent_Identity=48.7414187643021, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45553816, Length=437, Percent_Identity=48.7414187643021, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651721, Length=437, Percent_Identity=48.7414187643021, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI17864154, Length=437, Percent_Identity=48.7414187643021, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI17137380, Length=429, Percent_Identity=34.2657342657343, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI45550900, Length=437, Percent_Identity=33.1807780320366, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI281363316, Length=423, Percent_Identity=33.806146572104, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI17864358, Length=423, Percent_Identity=33.806146572104, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI19921738, Length=416, Percent_Identity=30.2884615384615, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI19922690, Length=316, Percent_Identity=29.1139240506329, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19922512, Length=447, Percent_Identity=24.3847874720358, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654852, Length=447, Percent_Identity=24.3847874720358, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647046, Length=373, Percent_Identity=23.3243967828418, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647048, Length=373, Percent_Identity=23.3243967828418, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI78706572, Length=138, Percent_Identity=37.6811594202899, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28572034, Length=171, Percent_Identity=34.5029239766082, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=108, Percent_Identity=35.1851851851852, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 46125; Mature: 46125

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVISIYTKRGIIMAKTKEHINLAFIGHVDHGKSTLVGHLLLKAGAIAEQQLDDGENKFR
CCEEEEEECCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHH
FVMDKLGEERERGVTIDLAHQKFSTKKYDYTVVDCPGHRDFVKNMITGASQADAGVLVVA
HHHHHHCCHHHCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEE
ADDGVMPQTKEHVFLSKTLGINQLIVAINKIDLVDYDEAKFNELKDEVSALIKTVGFNPA
ECCCCCCCCCCEEEEEHHCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
DVPFIPVSAFEGDNIKDASPNTSWYKGDTLMQALDNLAAPEKPVSLPLRIPIQDVYSITG
CCCEEEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCCCCCEEECCHHHHHEECC
VGTVPVGRVETGVMKKGENVIFEPAGASGEVKSIEMHHETFETAEPGDNIGFNVRGVGKN
CCCCCCCCCCHHHHCCCCEEEEECCCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEECCCC
DIRRGDVAGHVDDAPAVAKEFDAQIVVLQHPGVITVGYTPVFHCHTSQVACTFLELTAKL
CCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCEEEECCCEEEEEECHHHHHEEEEHHHCC
DPATGQVAEENPDFLKTGNAAFVKVKPTKPMVIENAKKIPQMGRFAIRDMGQTVAAGLCI
CCCCCCCCCCCCCCEECCCEEEEEEECCCCCEECCHHHCHHHHHHHHHHHHHHHHCCEEE
DVTPAK
EECCCC
>Mature Secondary Structure
MNVISIYTKRGIIMAKTKEHINLAFIGHVDHGKSTLVGHLLLKAGAIAEQQLDDGENKFR
CCEEEEEECCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCHHHH
FVMDKLGEERERGVTIDLAHQKFSTKKYDYTVVDCPGHRDFVKNMITGASQADAGVLVVA
HHHHHHCCHHHCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEE
ADDGVMPQTKEHVFLSKTLGINQLIVAINKIDLVDYDEAKFNELKDEVSALIKTVGFNPA
ECCCCCCCCCCEEEEEHHCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
DVPFIPVSAFEGDNIKDASPNTSWYKGDTLMQALDNLAAPEKPVSLPLRIPIQDVYSITG
CCCEEEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCCCCCEEECCHHHHHEECC
VGTVPVGRVETGVMKKGENVIFEPAGASGEVKSIEMHHETFETAEPGDNIGFNVRGVGKN
CCCCCCCCCCHHHHCCCCEEEEECCCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEECCCC
DIRRGDVAGHVDDAPAVAKEFDAQIVVLQHPGVITVGYTPVFHCHTSQVACTFLELTAKL
CCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCEEEECCCEEEEEECHHHHHEEEEHHHCC
DPATGQVAEENPDFLKTGNAAFVKVKPTKPMVIENAKKIPQMGRFAIRDMGQTVAAGLCI
CCCCCCCCCCCCCCEECCCEEEEEEECCCCCEECCHHHCHHHHHHHHHHHHHHHHCCEEE
DVTPAK
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA