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The map label for this gene is leuD [H]

Identifier: 148642907

GI number: 148642907

Start: 803847

End: 804332

Strand: Reverse

Name: leuD [H]

Synonym: Msm_0847

Alternate gene names: 148642907

Gene position: 804332-803847 (Counterclockwise)

Preceding gene: 148642908

Following gene: 148642906

Centisome position: 43.4

GC content: 31.48

Gene sequence:

>486_bases
ATGGATATAATTAAAGGAAAAACATGGACTTTTGGTGAAAATATTGATACGGATGTTATTATTCCCGGAAGATATTTGAG
AACCTTTAATCCTCAAGATTTGGCTGATCATGTACTTGAAGGAGAACGTCCAGATTTTACAAAGAATGTTAAAAAAGGAG
ACATAATAGTAGCTGATGAAAATTTTGGTTGCGGTTCATCAAGAGAGCAGGCTCCAGTAGCTATTAAAACTGCAGGTGTA
GATGCTATTGTGGCTAAATCTTTCGCCAGAATTTTTTACAGAAATGCAATTAACATTGGATTGCCAGTAATTGTTTGTGA
TATTCAGGCAAAAGACGGAGATATTATAAACATTGATTTGTCAAAAGGAATATTAACTAATGAAACTACTGGTGAATCAG
TTACTTTTGAACCTTTTAAAGAGTTTATGTTAGATATTTTAGAAGATAATGGTCTTGTTAATCATTATCTAAAAGAAAAA
CAATAA

Upstream 100 bases:

>100_bases
CATAGTTGAAGAGATTAAAGAGTATGTCGGAAACAGAGGCATAGTTCTTACAACTAAAGCACAAAATTATGGTCATGAAA
TCCAAAAATAAGTGGTATAT

Downstream 100 bases:

>100_bases
TTTTTATTTTTTGGAGGCATTAATAATGGAGTGTCCAATTTGCGGTTCTGATGATTTTGATATTTTAAATTCAAAGCAGA
AATCATCTAAAAAGAAGATT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MDIIKGKTWTFGENIDTDVIIPGRYLRTFNPQDLADHVLEGERPDFTKNVKKGDIIVADENFGCGSSREQAPVAIKTAGV
DAIVAKSFARIFYRNAINIGLPVIVCDIQAKDGDIINIDLSKGILTNETTGESVTFEPFKEFMLDILEDNGLVNHYLKEK
Q

Sequences:

>Translated_161_residues
MDIIKGKTWTFGENIDTDVIIPGRYLRTFNPQDLADHVLEGERPDFTKNVKKGDIIVADENFGCGSSREQAPVAIKTAGV
DAIVAKSFARIFYRNAINIGLPVIVCDIQAKDGDIINIDLSKGILTNETTGESVTFEPFKEFMLDILEDNGLVNHYLKEK
Q
>Mature_161_residues
MDIIKGKTWTFGENIDTDVIIPGRYLRTFNPQDLADHVLEGERPDFTKNVKKGDIIVADENFGCGSSREQAPVAIKTAGV
DAIVAKSFARIFYRNAINIGLPVIVCDIQAKDGDIINIDLSKGILTNETTGESVTFEPFKEFMLDILEDNGLVNHYLKEK
Q

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=110, Percent_Identity=39.0909090909091, Blast_Score=74, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=92, Percent_Identity=41.304347826087, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321429, Length=111, Percent_Identity=36.9369369369369, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 17891; Mature: 17891

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIKGKTWTFGENIDTDVIIPGRYLRTFNPQDLADHVLEGERPDFTKNVKKGDIIVADE
CCCCCCCCEECCCCCCCCEEECCHHEECCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
NFGCGSSREQAPVAIKTAGVDAIVAKSFARIFYRNAINIGLPVIVCDIQAKDGDIINIDL
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEC
SKGILTNETTGESVTFEPFKEFMLDILEDNGLVNHYLKEKQ
CCCEEECCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MDIIKGKTWTFGENIDTDVIIPGRYLRTFNPQDLADHVLEGERPDFTKNVKKGDIIVADE
CCCCCCCCEECCCCCCCCEEECCHHEECCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
NFGCGSSREQAPVAIKTAGVDAIVAKSFARIFYRNAINIGLPVIVCDIQAKDGDIINIDL
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEC
SKGILTNETTGESVTFEPFKEFMLDILEDNGLVNHYLKEKQ
CCCEEECCCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]