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The map label for this gene is thsB [H]

Identifier: 148642886

GI number: 148642886

Start: 784085

End: 785704

Strand: Reverse

Name: thsB [H]

Synonym: Msm_0826

Alternate gene names: 148642886

Gene position: 785704-784085 (Counterclockwise)

Preceding gene: 148642888

Following gene: 148642885

Centisome position: 42.4

GC content: 37.16

Gene sequence:

>1620_bases
ATGGCACAAGGTGGACAACCAATTTTTATTTTACCTGAAGGAACAAACAGGTCTGTCGGAAGAGATGCTCAAAGAAACAA
TATTTTAGCAGGAAAAGTACTTGCTGAAACCGTAAGAACCACATTAGGTCCAAAAGGAATGGACAAAATGTTAGTGGACG
GACTTGGAGATATCGTTGTAACCAATGACGGTGTTACAATCTTAAAAGAAATGGATATCGAACATCCTGCAGCTAAAATG
TTAGTGGAAGTAGCTAAAACTCAAGAAGACGAAGTTGGAGATGGAACTACTACTGCAGTAATCATTGCTGGAGAATTATT
GAAAAAATCCGAATCTTTATTAGATCAAGATATTCACCCAACAATCATTGCAATGGGATACAGACAAGCAGCTGAAAAAG
CACAAGAAATCTTAGATGATATTGCAATCGATTCAGTTGATGAAGAAACTTTAATTAAAGTAGCTATGACTGCTATGACT
GGTAAAGGAACTGAAGCAGCACGTGAACCATTAGCAAAATTAATCGTAGATGCAGTTCAAAAAGTAGCTGAAGACGGTGC
AGTAGACACTGATAATATTAAAATTGAGAAAAAAGACGGTGCTGTTGTAGAAGACTCTACTTTAGTTGAAGGAGTAATTG
TTGACAAAGAAAGAGTACACCCAGGTATGCCTTCTGAAGTTAAAGATGCAAAAATCGCATTAGTTAACTCTCCTCTTGAA
GTAAAAGAAACTGAAGTTGATGCAGAAATCAGAATAACTGACCCTGCTCAAATGCAAGCTTTCATTGAACAAGAAGAAAA
AATGGTTAAAGACATGGTAGATAAAGTAGCTGAATCCGGTGCTAATGTATTATTCGCACAAAAAGGTATTGACGATTTAG
CACAACATTACTTATCTAAAGCAGGTATTTTAGCTGTAAGAAGGGTTAAAAAATCAGATATTGAAAAATTAGCTAGAGCT
ACTGGTGCTAATGTAGTAACTAACTTAGAAGACTTAACTGCTGACGATTTAGGTGAAGCAGGTATTGTAGAAGAAAGAAA
AGTATCTGGCGAAGAAATGATCTTTGTAGAAGAATGCAGTGTAGCTAAATCTGTAACCTTATTCGTAAGAGGAAGTACCA
AACACATTGTTGATGAAATCGTAAGAGCTATTGAAGACGCAATTGGTGTTGTAGCAGCTACTGTTGAAGATGACAAAGTT
GTAGCTGGTGGAGGAGCTCCTGAAATTGCAATGGCTAAAAAACTCAAAGACTATGCTGATTCAATCAGTGGAAGAGAACA
ATTAGCTGTAAATGCATTTGCAGAAGCTTTAGAAATTGTTCCTAAAACCTTAGCTGAAAATGCAGGTTTAGACAGTATAG
ATTCATTAGTAGATTTAAGAGCTGCTCATGAAAACAGTGCTGTAATGGGATTAGATGTATTTACCGGTAAAGTAGCAGAC
ATGAAAGAAGCTGGTGTAATTGAACCTAAACGTGTCAAAAAACAAGCTATCCAATCTGCTTCTGAAGCAGCTGAAATGAT
TTTAAGAATTGATGACGTAATCGCATCTAGCGGTAAAGGTGATGCTGACATGGGCGGTATGGATCCTGCTGCTATGGGCG
GTATGCCTCCAATGATGTAA

Upstream 100 bases:

>100_bases
TCACTATATGGTAGAAGGGTTATTCAACAATATAATTATTTTTTATGTTGATATTTAAACTGTTCTATAAATAATAAATT
AATTTTAAAAGGTGATTTTA

Downstream 100 bases:

>100_bases
ATCTAAAAGAAATTATTTAATTTCTTTTTTTCTTTTTATTTTTAAGGTATTATCTTGCAAGCTAAACGACTTATTTTTAA
AAATTCAACTGGAGACAGAG

Product: thermosome

Products: NA

Alternate protein names: Chaperonin subunit beta; Thermosome subunit 2 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAQGGQPIFILPEGTNRSVGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEMDIEHPAAKM
LVEVAKTQEDEVGDGTTTAVIIAGELLKKSESLLDQDIHPTIIAMGYRQAAEKAQEILDDIAIDSVDEETLIKVAMTAMT
GKGTEAAREPLAKLIVDAVQKVAEDGAVDTDNIKIEKKDGAVVEDSTLVEGVIVDKERVHPGMPSEVKDAKIALVNSPLE
VKETEVDAEIRITDPAQMQAFIEQEEKMVKDMVDKVAESGANVLFAQKGIDDLAQHYLSKAGILAVRRVKKSDIEKLARA
TGANVVTNLEDLTADDLGEAGIVEERKVSGEEMIFVEECSVAKSVTLFVRGSTKHIVDEIVRAIEDAIGVVAATVEDDKV
VAGGGAPEIAMAKKLKDYADSISGREQLAVNAFAEALEIVPKTLAENAGLDSIDSLVDLRAAHENSAVMGLDVFTGKVAD
MKEAGVIEPKRVKKQAIQSASEAAEMILRIDDVIASSGKGDADMGGMDPAAMGGMPPMM

Sequences:

>Translated_539_residues
MAQGGQPIFILPEGTNRSVGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEMDIEHPAAKM
LVEVAKTQEDEVGDGTTTAVIIAGELLKKSESLLDQDIHPTIIAMGYRQAAEKAQEILDDIAIDSVDEETLIKVAMTAMT
GKGTEAAREPLAKLIVDAVQKVAEDGAVDTDNIKIEKKDGAVVEDSTLVEGVIVDKERVHPGMPSEVKDAKIALVNSPLE
VKETEVDAEIRITDPAQMQAFIEQEEKMVKDMVDKVAESGANVLFAQKGIDDLAQHYLSKAGILAVRRVKKSDIEKLARA
TGANVVTNLEDLTADDLGEAGIVEERKVSGEEMIFVEECSVAKSVTLFVRGSTKHIVDEIVRAIEDAIGVVAATVEDDKV
VAGGGAPEIAMAKKLKDYADSISGREQLAVNAFAEALEIVPKTLAENAGLDSIDSLVDLRAAHENSAVMGLDVFTGKVAD
MKEAGVIEPKRVKKQAIQSASEAAEMILRIDDVIASSGKGDADMGGMDPAAMGGMPPMM
>Mature_538_residues
AQGGQPIFILPEGTNRSVGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVVTNDGVTILKEMDIEHPAAKML
VEVAKTQEDEVGDGTTTAVIIAGELLKKSESLLDQDIHPTIIAMGYRQAAEKAQEILDDIAIDSVDEETLIKVAMTAMTG
KGTEAAREPLAKLIVDAVQKVAEDGAVDTDNIKIEKKDGAVVEDSTLVEGVIVDKERVHPGMPSEVKDAKIALVNSPLEV
KETEVDAEIRITDPAQMQAFIEQEEKMVKDMVDKVAESGANVLFAQKGIDDLAQHYLSKAGILAVRRVKKSDIEKLARAT
GANVVTNLEDLTADDLGEAGIVEERKVSGEEMIFVEECSVAKSVTLFVRGSTKHIVDEIVRAIEDAIGVVAATVEDDKVV
AGGGAPEIAMAKKLKDYADSISGREQLAVNAFAEALEIVPKTLAENAGLDSIDSLVDLRAAHENSAVMGLDVFTGKVADM
KEAGVIEPKRVKKQAIQSASEAAEMILRIDDVIASSGKGDADMGGMDPAAMGGMPPMM

Specific function: Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TCP-1 chaperonin family [H]

Homologues:

Organism=Homo sapiens, GI63162572, Length=543, Percent_Identity=38.3057090239411, Blast_Score=397, Evalue=1e-111,
Organism=Homo sapiens, GI24307939, Length=521, Percent_Identity=41.0748560460653, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI38455427, Length=502, Percent_Identity=40.6374501992032, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI5453607, Length=518, Percent_Identity=38.996138996139, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI57863257, Length=522, Percent_Identity=37.9310344827586, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI58761484, Length=543, Percent_Identity=34.2541436464088, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI58331173, Length=500, Percent_Identity=38.4, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI4502643, Length=509, Percent_Identity=37.721021611002, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI261399877, Length=478, Percent_Identity=37.8661087866109, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI5453603, Length=520, Percent_Identity=33.2692307692308, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI302058290, Length=492, Percent_Identity=36.7886178861789, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI261399875, Length=433, Percent_Identity=35.796766743649, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI302058292, Length=500, Percent_Identity=34.4, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI58331171, Length=509, Percent_Identity=33.7917485265226, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI48762932, Length=536, Percent_Identity=34.7014925373134, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI57863259, Length=364, Percent_Identity=34.6153846153846, Blast_Score=215, Evalue=9e-56,
Organism=Homo sapiens, GI58331185, Length=314, Percent_Identity=36.624203821656, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI7657253, Length=491, Percent_Identity=25.8655804480652, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI41399285, Length=543, Percent_Identity=24.4935543278085, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI31542947, Length=543, Percent_Identity=24.4935543278085, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1790586, Length=556, Percent_Identity=24.2805755395683, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144674, Length=521, Percent_Identity=41.0748560460653, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17532601, Length=536, Percent_Identity=38.0597014925373, Blast_Score=354, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI25148561, Length=528, Percent_Identity=35.4166666666667, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17532603, Length=504, Percent_Identity=39.0873015873016, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI17564182, Length=516, Percent_Identity=35.2713178294574, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25147750, Length=527, Percent_Identity=36.4326375711575, Blast_Score=307, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI25144678, Length=518, Percent_Identity=36.6795366795367, Blast_Score=302, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI32566944, Length=440, Percent_Identity=34.0909090909091, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI25144680, Length=395, Percent_Identity=36.7088607594937, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71998178, Length=549, Percent_Identity=30.9653916211293, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71981457, Length=325, Percent_Identity=40.6153846153846, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17555558, Length=563, Percent_Identity=25.7548845470693, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322446, Length=525, Percent_Identity=35.8095238095238, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6322524, Length=532, Percent_Identity=37.0300751879699, Blast_Score=332, Evalue=8e-92,
Organism=Saccharomyces cerevisiae, GI6322350, Length=541, Percent_Identity=35.1201478743068, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6320418, Length=524, Percent_Identity=37.0229007633588, Blast_Score=326, Evalue=6e-90,
Organism=Saccharomyces cerevisiae, GI6320058, Length=515, Percent_Identity=36.1165048543689, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6322049, Length=486, Percent_Identity=34.156378600823, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320393, Length=523, Percent_Identity=31.3575525812619, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322452, Length=540, Percent_Identity=31.4814814814815, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6323288, Length=544, Percent_Identity=22.4264705882353, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17647245, Length=521, Percent_Identity=41.0748560460653, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24647512, Length=555, Percent_Identity=38.5585585585586, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24647510, Length=555, Percent_Identity=38.5585585585586, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24652903, Length=500, Percent_Identity=41.2, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24649027, Length=551, Percent_Identity=37.7495462794918, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24649029, Length=551, Percent_Identity=37.7495462794918, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24645179, Length=527, Percent_Identity=37.5711574952562, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24583944, Length=513, Percent_Identity=37.2319688109162, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI28571140, Length=521, Percent_Identity=35.1247600767754, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI18858175, Length=521, Percent_Identity=35.1247600767754, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI18859933, Length=502, Percent_Identity=36.2549800796813, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI19921848, Length=510, Percent_Identity=32.5490196078431, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24641193, Length=533, Percent_Identity=25.515947467167, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24641191, Length=533, Percent_Identity=25.515947467167, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45550936, Length=526, Percent_Identity=25.2851711026616, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550132, Length=526, Percent_Identity=25.2851711026616, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550935, Length=526, Percent_Identity=25.2851711026616, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17864606, Length=566, Percent_Identity=23.1448763250883, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584129, Length=530, Percent_Identity=22.0754716981132, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19921262, Length=530, Percent_Identity=22.0754716981132, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017998
- InterPro:   IPR002194
- InterPro:   IPR002423
- InterPro:   IPR012714 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57255; Mature: 57124

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00750 TCP1_1 ; PS00751 TCP1_2 ; PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGGQPIFILPEGTNRSVGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVV
CCCCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEE
TNDGVTILKEMDIEHPAAKMLVEVAKTQEDEVGDGTTTAVIIAGELLKKSESLLDQDIHP
ECCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCC
TIIAMGYRQAAEKAQEILDDIAIDSVDEETLIKVAMTAMTGKGTEAAREPLAKLIVDAVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KVAEDGAVDTDNIKIEKKDGAVVEDSTLVEGVIVDKERVHPGMPSEVKDAKIALVNSPLE
HHHHCCCCCCCCEEEECCCCCEEECCHHHHEEEECHHHCCCCCCCCCCCCEEEEECCCCC
VKETEVDAEIRITDPAQMQAFIEQEEKMVKDMVDKVAESGANVLFAQKGIDDLAQHYLSK
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
AGILAVRRVKKSDIEKLARATGANVVTNLEDLTADDLGEAGIVEERKVSGEEMIFVEECS
HHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHCHHHCCCCCCCHHHHCCCCCEEEEEHHC
VAKSVTLFVRGSTKHIVDEIVRAIEDAIGVVAATVEDDKVVAGGGAPEIAMAKKLKDYAD
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCHHHHHHHHHHHHH
SISGREQLAVNAFAEALEIVPKTLAENAGLDSIDSLVDLRAAHENSAVMGLDVFTGKVAD
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEHHHCCCHHC
MKEAGVIEPKRVKKQAIQSASEAAEMILRIDDVIASSGKGDADMGGMDPAAMGGMPPMM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
AQGGQPIFILPEGTNRSVGRDAQRNNILAGKVLAETVRTTLGPKGMDKMLVDGLGDIVV
CCCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEE
TNDGVTILKEMDIEHPAAKMLVEVAKTQEDEVGDGTTTAVIIAGELLKKSESLLDQDIHP
ECCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCC
TIIAMGYRQAAEKAQEILDDIAIDSVDEETLIKVAMTAMTGKGTEAAREPLAKLIVDAVQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KVAEDGAVDTDNIKIEKKDGAVVEDSTLVEGVIVDKERVHPGMPSEVKDAKIALVNSPLE
HHHHCCCCCCCCEEEECCCCCEEECCHHHHEEEECHHHCCCCCCCCCCCCEEEEECCCCC
VKETEVDAEIRITDPAQMQAFIEQEEKMVKDMVDKVAESGANVLFAQKGIDDLAQHYLSK
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
AGILAVRRVKKSDIEKLARATGANVVTNLEDLTADDLGEAGIVEERKVSGEEMIFVEECS
HHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHCHHHCCCCCCCHHHHCCCCCEEEEEHHC
VAKSVTLFVRGSTKHIVDEIVRAIEDAIGVVAATVEDDKVVAGGGAPEIAMAKKLKDYAD
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCHHHHHHHHHHHHH
SISGREQLAVNAFAEALEIVPKTLAENAGLDSIDSLVDLRAAHENSAVMGLDVFTGKVAD
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEHHHCCCHHC
MKEAGVIEPKRVKKQAIQSASEAAEMILRIDDVIASSGKGDADMGGMDPAAMGGMPPMM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]