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The map label for this gene is truB [H]

Identifier: 148642792

GI number: 148642792

Start: 705911

End: 706873

Strand: Reverse

Name: truB [H]

Synonym: Msm_0732

Alternate gene names: 148642792

Gene position: 706873-705911 (Counterclockwise)

Preceding gene: 148642793

Following gene: 148642791

Centisome position: 38.14

GC content: 35.1

Gene sequence:

>963_bases
ATGAAAGACTTTCTTACAAAATCCAAAAGTTTTACAAATCCTGAATATGGATGTGCTCCTGAAGAACGTGAAATTTCAGA
TTATATTTCTCATGGTGTTATTAATTTAGATAAACCGTCAGGACCAACTTCTCATGAAATTGATTCATGGGTAAAACGTA
TTTTAAATTTGGAGAAATCAGGACACGGCGGAACTTTGGATCCAAAAGTTACAGGTGTTTTGCCTGTAGGACTTGGAGAT
GCAACAAGAGCTATTCAACTTCTTTTAACAGCTCCTAAAGAATATGTTTGTGTAATGACTTTTCATCATGAAGTTGATGA
GGATAAAATTCGTGAAGTGTTTAATGAGTTTACAGGTAAAATTTTCCAACTTCCTCCGGTTAAATCAGCAGTTAAACGTG
AATTAAGATCACGTAATGTTTATTATTCCACTATCTATGAAATTAATGGTAGGGATGTCTTATTTAGAATAGGCTGTGAA
GCTGGAACTTACGTTAGAACTTACTGCCATAATATAGGTGAAGCAATTGGTGTTGGTGCACACATGGCAGAGCTTAGAAG
AACACAGGTAGGTTCATTTACAGAGGATAACCATTTATCCACTCTTCAGGATGTTACTGATGCATATCATTTCTACAAAG
AAGATGGTGACGAGTCATTTTTAAGAAAAGCTATTATGCCGATGGAGCGAGCTGCAGATTACCTGCCAAAAGTAGTTATT
AAAGATTCTGCAGTTGATGCAATATGTCACGGTGCCAACTTGGCCAGTGGGGGAATAGCTTATCTGTCTGACAATATTCA
AAAAAATTCCATAGTAGCTATTGAAACACTTAAAGGTGAACTGGTTGCTTCAGGTAATTCTTTATTTAGCAGTGATGAGA
TTTTAGATTTGGAATCAGGATTTGCTGTTAATGTTTCTAAAGTTTTTATGAAAACAGATATTTATCCTAAAATGTGGAAA
TAA

Upstream 100 bases:

>100_bases
AGCAGCTTTAGCTGATTTATAAATGGATAATTAATTAAATTATTCATTACTTATTTTTATTTACTTATTTTTTTTATTTA
TTGATTTTTAGTGATTTTTC

Downstream 100 bases:

>100_bases
ATTATTAAAAACTTTATTTTTTTATTTTTAATTAGATTTACTTTTTTTTGTAAGAAATATATTAATAACTTTCAAATTAC
TTATATACATTAAATAAGAA

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKDFLTKSKSFTNPEYGCAPEEREISDYISHGVINLDKPSGPTSHEIDSWVKRILNLEKSGHGGTLDPKVTGVLPVGLGD
ATRAIQLLLTAPKEYVCVMTFHHEVDEDKIREVFNEFTGKIFQLPPVKSAVKRELRSRNVYYSTIYEINGRDVLFRIGCE
AGTYVRTYCHNIGEAIGVGAHMAELRRTQVGSFTEDNHLSTLQDVTDAYHFYKEDGDESFLRKAIMPMERAADYLPKVVI
KDSAVDAICHGANLASGGIAYLSDNIQKNSIVAIETLKGELVASGNSLFSSDEILDLESGFAVNVSKVFMKTDIYPKMWK

Sequences:

>Translated_320_residues
MKDFLTKSKSFTNPEYGCAPEEREISDYISHGVINLDKPSGPTSHEIDSWVKRILNLEKSGHGGTLDPKVTGVLPVGLGD
ATRAIQLLLTAPKEYVCVMTFHHEVDEDKIREVFNEFTGKIFQLPPVKSAVKRELRSRNVYYSTIYEINGRDVLFRIGCE
AGTYVRTYCHNIGEAIGVGAHMAELRRTQVGSFTEDNHLSTLQDVTDAYHFYKEDGDESFLRKAIMPMERAADYLPKVVI
KDSAVDAICHGANLASGGIAYLSDNIQKNSIVAIETLKGELVASGNSLFSSDEILDLESGFAVNVSKVFMKTDIYPKMWK
>Mature_320_residues
MKDFLTKSKSFTNPEYGCAPEEREISDYISHGVINLDKPSGPTSHEIDSWVKRILNLEKSGHGGTLDPKVTGVLPVGLGD
ATRAIQLLLTAPKEYVCVMTFHHEVDEDKIREVFNEFTGKIFQLPPVKSAVKRELRSRNVYYSTIYEINGRDVLFRIGCE
AGTYVRTYCHNIGEAIGVGAHMAELRRTQVGSFTEDNHLSTLQDVTDAYHFYKEDGDESFLRKAIMPMERAADYLPKVVI
KDSAVDAICHGANLASGGIAYLSDNIQKNSIVAIETLKGELVASGNSLFSSDEILDLESGFAVNVSKVFMKTDIYPKMWK

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI215599015, Length=316, Percent_Identity=40.8227848101266, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI4503337, Length=316, Percent_Identity=40.8227848101266, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI21040257, Length=219, Percent_Identity=28.310502283105, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI2367200, Length=253, Percent_Identity=30.4347826086957, Blast_Score=104, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17553978, Length=316, Percent_Identity=39.2405063291139, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6323204, Length=319, Percent_Identity=42.6332288401254, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI281364189, Length=316, Percent_Identity=39.5569620253165, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI281364187, Length=316, Percent_Identity=39.5569620253165, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI281364185, Length=316, Percent_Identity=39.5569620253165, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI281364183, Length=316, Percent_Identity=39.5569620253165, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI62471759, Length=316, Percent_Identity=39.5569620253165, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17975520, Length=316, Percent_Identity=39.5569620253165, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35611; Mature: 35611

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFLTKSKSFTNPEYGCAPEEREISDYISHGVINLDKPSGPTSHEIDSWVKRILNLEKS
CCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCC
GHGGTLDPKVTGVLPVGLGDATRAIQLLLTAPKEYVCVMTFHHEVDEDKIREVFNEFTGK
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
IFQLPPVKSAVKRELRSRNVYYSTIYEINGRDVLFRIGCEAGTYVRTYCHNIGEAIGVGA
EEECCCHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHH
HMAELRRTQVGSFTEDNHLSTLQDVTDAYHFYKEDGDESFLRKAIMPMERAADYLPKVVI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCEEE
KDSAVDAICHGANLASGGIAYLSDNIQKNSIVAIETLKGELVASGNSLFSSDEILDLESG
CCCHHHHHHCCCCCCCCCCEEHCCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEECCCC
FAVNVSKVFMKTDIYPKMWK
CEEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKDFLTKSKSFTNPEYGCAPEEREISDYISHGVINLDKPSGPTSHEIDSWVKRILNLEKS
CCCHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCC
GHGGTLDPKVTGVLPVGLGDATRAIQLLLTAPKEYVCVMTFHHEVDEDKIREVFNEFTGK
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
IFQLPPVKSAVKRELRSRNVYYSTIYEINGRDVLFRIGCEAGTYVRTYCHNIGEAIGVGA
EEECCCHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHH
HMAELRRTQVGSFTEDNHLSTLQDVTDAYHFYKEDGDESFLRKAIMPMERAADYLPKVVI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCEEE
KDSAVDAICHGANLASGGIAYLSDNIQKNSIVAIETLKGELVASGNSLFSSDEILDLESG
CCCHHHHHHCCCCCCCCCCEEHCCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEECCCC
FAVNVSKVFMKTDIYPKMWK
CEEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]